The hypoxic pulmonary vasoconstriction (HPV) in Yunan monkey has been observed. The results showed that acute alveolar hypoxia caused significant pulmonary hypertension, associated w~th art increase in pulmonary vascular resistance. The maximal response appeared 3 min after onset of hypoxia. Monkey may be proposed as moderate responder in HPV. The HPV in monkey was blocked by lipoxygenase inhibitor diethylcarbamazine and enhanced by cyclooxy genase blocker...indomethacin. It is suggested that lipoxygenase metabolites leukotrienes probably play the role of a mediator in HPV of monkey, whereas cyclooxygenase metabolites prostaglan dins act as medulator.

Modiastinoscopy is an invasive method with very high sensitivity and specificity, which has an unreplaceble role in diagnosis of indeterminate mediastinal masses and staging of lung neoplasms. Video me-diastinoscopy provides optimal visualization of mediasfinal structure and the possibility for the surgeon to operatewith both hands. And more operation can be performed by video mediastinoscopy. Now video mediastinoscopy has been replacing traditional mediastinoscopy gradually.

Objeetive To observe the effect of the fire and leopard-spot needling therapy in treating ankle sprain.Methods We randomly selected 59 ankle sprain cascs and divided them into 2 groups,with one contained 30 cases treated hy fire and leopard-spot needling therapy as the treatment group,and another contained 29 cases treated by computerized multa-function therapy apparatus as the control group.After 7 days'treatment,we observed the effects of each group.Results The results snowed that the effect of the treatment group was much better than the control group.Conclusion With the features of collateral vessel pricking and warming and activating meridians,fire and leopard-spot needling therapy can remove swelling and relieve pain rapidly.

Objective:To observe the effect of subtle adjustment of the cervical spine combined with Shu Jing Ding Xuan (Neck-relaxing and Dizziness-alleviating) Decoction for cervical vertigo.
Methods:An open-label, randomized and parallel controlled clinical study was designed. Subtle adjustment of the cervical spine combined with Shu Jing Ding Xuan (Neck-relaxing and Dizziness-alleviating) Decoction was used for patients in the treatment group, whereas subtle adjustment alone was used for patients in the control group. The results in the two groups were statistically analyzed after two courses of treatment.
Results:The recovery rate and total effective rate in the treatment group were 20.6%and 88.2%respectively, versus 14.7%and 73.5%in the control group. The inter-group differences were statistically significant (both P<0.01).
Conclusion: Subtle adjustment of the cervical spine combined with Shu Jing Ding Xuan (Neck-relaxing and Dizziness-alleviating) Decoction is better than subtle adjustment alone for cervical vertigo.

OBJECTIVE:To study the pharmacokinetic features of Fagopyrum tataricum total flavonoids. METHODS:6 Beagle dogs were intragastrically given single dose of 80 mg F.tataricum total flavonoids (with content of quercetin at 1.2% and the content of eldrin at 24.7%). HPLC-UV spectrophotometry was adopted to determine the content of total quercetin in the hydrolyzed plasma samples. RESULTS:Taking the total quercetin as the index of the pharmacokinetic features of the F. tataricum total flavonoids in the Beagle dogs,the main pharmacokinetic parameters were as follows:AUC0→t:(9.92?1.49)?g?h?mL-1;AUC0→∞:(10.81?1.86)?g?h?mL-1;Cmax:(1.07?0.19)?g?mL-1. CONCLUSION:The pharmacokinetics of the F.tataricum total flavonoids in the Beagle dogs conforms to two-compartment model,and the concentration-time curves are characterized by multiple peak pharmacokinetics.

The reproductive toxicity of environmental estrogens(EEs)has become an extensively studied subject in environmental science and reproductive medicine nowadays.Many epidemiology data and experimental evidences indicated that EEs may significantly cause the disorders of male sex differentiation such as microphallus,hypospadia,cryptorchidism and masculinization behavior disorder and so on.The mechanism includes estrogenic activity,anti-androgenic activity as well as other feedback regulation and signal transmission which influence hormones metabolism.

[Objective]To review the antitumor progress of Raddeanin A in nearly 10 years. [Method] Col ecting the antitumor research literature about Raddeanin A for nearly 10 years, describing its antitumor research mechanism, such as inducting of cellapoptosis blocking the cellcycle, blocking STATs signaling pathways and inhibiting the tumor angiogenesis. [Result] Raddeanin A could induce the H460 cellapoptosis, block LoVo cellcycle and STATs signaling pathways of LoVo cells, and inhibit HepG-2 cellangiogenesis. [Conclusion] The antitumor mechanism of Raddeanin A could be inducting of cellapoptosis, blocking the cellcycle, blocking STATs signaling pathways and inhibiting the tumor angiogenesis.

Objective To study the character of transformation of H.pylori into coccoid form under oxidative stress, and the changes in the biological charasteristics after transformation. Methods The coccoid form of H.pylori was studied with bacteriological, enymological, and genetical methods to identify its differences from the original form. Animal experiment was conducted to determine its implantation capacity. Results Morphological transformation from spiral to coccoid form occurred with the prolongation of culture under oxygen stress. The metabolic activity was reduced gradually, finally maintained at a lower level. The activities of urease, alkaline phosphatase and acid phosphatase were decreased markedly. The mRNA levels of main virulence factors were declined. Both mRNA and activities of enzyme of Sod B and Kat retained high levels. A part of the coccoid forms showed reversion to helical form, and colonization in mice stomach could be found after being inoculated to mice. Conclusions The metabolism and virulence of H.pylori were all lowered under oxidative stress, but a part of the coccoid form may be viable. Sod B and Kat may play an important role in the oxygen metabolism of H.pylori.

AIM: To study whether agonists of group II and III metabotropic glutamate receptors (mGluRs) exert effects on LPS-induced glutamate uptake inhibition in C6 glioma cells. METHODS: The glutamate uptake into C6 glioma cells was measured by uptake of [3H]-D,L-glutamate; and the apoptosis and the viability of C6 glioma cells were investigated by Hoechst33342 and MTT methods, respectively. RESULTS: LPS (4, 6 ?g/mL) inhibited glutamate uptake significantly compared with that in the control group without effect on the apoptosis and viability of C6 glioma cells. Pretreatment of C6 glioma cells with group II and III mGluRs agonists DCG-IV(100 ?mol/L) and L-AP4(100 ?mol/L) reversed LPS-induced glutamate uptake inhibition. These recovery effects were abolished by their respective antagonists APICA and MSOP. CONCLUSION: Activation of group II and III mGluRs recovers LPS-induced glutamate uptake inhibition in C6 glioma cells, suggesting the enhancement of glutamate uptake is involved in neuroprotective roles exerted by group II and III mGluRs agonists.

Objective To study the effects of deep hypothermia and L-arginine(NO precursor) pretreatment on cerebral protection after circulatory arrest and resuscitation in a canine model. Methods Ten healthy adult dogs of both sexes, weighing 14.45?2.3kg, were randomly divided into two groups(n=5): sham treatment group and L-arginine pretreatment group. Extracorporeal circulation was established routinely,circulatory arrest was induced when the nasopharyngeal temperature was reduced to 18℃, then the animals were resuscitated 90min later with extracorporeal circulation. SjvO2 was measured at 30min before circulatory arrest, 0min, 45min, 90min after circulatory arrest and at 60minutes after resuscitation. The ultrastructure changes in cerabral cortex were observed with transmission electron microscope (HITACHI 7500). The brain water content was also determined after the dogs were sacrificed. Results SjvO2 had decreased obviously after circulatory arrest, attaining the lowest level in CA 90min.After resuscitation it increased again in both groups. The levels of SjvO2 in dogs of L-arginine pretreatment group were markedly higher than that of control group at 45min and 90min after circulatory arrest (P