[Objective] To investigate the effect of electroacupuncture (EA) of Neiguan (PC6) poinl on rnyocardial fibrosis and myocardial contents of collagen type Ⅰ and type Ⅲ in rats with pressure overload. [ Methods ] Rats were randomized to four groups: pseudo-operation group ( group A), model group ( group B) , EA group ( group C) and western medicine group (group D). Pressure-overloaded rat models were established by restriction of abdominal aortic artery. Left ventricular mass index (LVMI) and blood pressure (BP) in the four groups were observed; the plasma level of angiotensin Ⅱ (Ang Ⅱ ) was detected by radioimmunoassay and myocardial contents of collagen type Ⅰ and type Ⅲ by immunohistochemical staining method. [Results] BP, LVMI, expression levels of Ang If and collagen type Ⅰ and type Ⅲ were increased in group B as compared with those in group A ( P 0.05) . [Conclusion] EA of Neiguan point is effective in preventing and treating myocardial fibrosis induced by pressure overload.

Medicine, Chinese Traditional ; Translations

Objective To investigate the expression of RCAS1,which is a receptor-binding cancer antigen expressed on SiSo cells in many tumor cell lines. Methods Using real-time polymerase chain reaction(PCR),Northern blot,immunohistochemical examination and enzyme-linked immunosorbent assay,we detected the expre-(ssion) of RCAS1 in 10 different tumor cell lines and two normal cell lines. Results The expression of RCAS1 in the tumor cell lines and the fetal renal cell line(293 cell) was detected by real-time PCR and Northern blot,but not in the normal liver cell line(LO2 cell).The results of immunohistochemical examination on the tumor cell lines and the 293 cell showed that RCAS1 existed in cytoplasma and on cell surface.Except HuH-7 cell and LO2 cell,the soluble RCAS1 protein in supernatants of the other cells was significantly more than that in DMEM(P

This paper introduces the risk classification and listing way of medical devices in the United States, and according to the contents in various situations, FDA provides the requirements for clinical evaluation. At the same time, through the comparative study on the similarities and differences between USA and our country of the clinical evaluation, the paper puts forward some suggestions.
Equipment and Supplies ; standards ; Risk Assessment ; United States ; United States Food and Drug Administration

Air Pollutants ; Cardiovascular Diseases ; epidemiology ; Humans ; Particulate Matter

Objective To design a new kind of wireless monitoring thermometer which can help the doctor to monitor the patient's temperature without touching them. Methods The system is based on a new kind of single chip MSP430F413,the thermistor was used in temperature detect part; the single chip's comparator A and timer A was used in A/D converter part to realize slop A/D; the NRF2401 chip was used in wireless transmission part; the system is connected with PC in the way of serial communications. Results The thermometer can work in -40 to 85 ℃ when the temperature is near animal heat,the thermometer is most accurate which precision is 0.1 ℃. Conclusion The system can measure the patient's temperature accurately,at the same time,the temperature data is sent to the receiving end in the way of wireless communication,and then data is sent to PC,which PC can record the temperature curve data and help the doctors to diagnose.

Objective: To investigate the expression of KAI1 gene in the urothelial cancer tissues and its relationship with the invasion and metastasis of urothelial cancer. Methods: The expression of KAI1 mRNA was detected by real-time fluorescent quantitative(RFQ-PCR) in urothelial cancer tissues and normal mucosa of urinary tract. The KAI1 protein expression was detected by immunohistochemistry(IHC) method in bladder transitional cell carcinoma tissues and the paired normal mucosal tissues. Results: QRT-PCR showed that the average level of KAI1 mRNA in the urothelial cancer tissues was significantly lower than that in the normal bladder tissues (P<0.01); moreover, the increase of pathological grades and clinical stages and the development of lymphatic metastasis were associated with the decrease of KAI1 expression, with significant difference found between the different groups(P<0.05 or P<0.01). The protein expression of KAI1 in the urothelial cancer tissues was significantly lower than that in the normal bladder tissues(P<0.01). The protein expression of KAI1 was decreased with the increase of pathological grades (P<0.05 or P<0.01). We also found that higher expression of KAI1 was associated with superficial invasion (P<0.05) and the presence of lymphatic metastasis (P<0.05). Conclusion: The down-regulation of KAI1 gene is associated with differentiation, infiltration, and lymphatic metastasis of urothelial cancer, which might serve as an effective indicator for malignancy, metastasis and prognosis of urothelial cancer.

Air Pollution ; Atherosclerosis ; etiology ; Humans ; Particulate Matter

Objective To understand the control of medical waste disposal in Changsha. Methods The kinds, output, collection and disposal of medical wastes were investigated in three general hospitals, one tumor hospital and one paediatric hospital in Changsha. Results The main components of medical wastes were glass, plastic and cotton, which took 55%, 20% and 12% of the total output respectively. The daily outputs of medical wastes were 0.48?0.63 and 0.43 kg per bed in three general hospitals, 0.58 and 0.71 kg per bed in paediatric hospital and tumor hospital respectively. Based on these data, the daily output of medical wastes in Changsha was estimated about 9.390 t. The recovery rate of medical wastes mainly including glass and plastic was 49.32%. The disposal rate of medical wastes only was 1.34%. The rest of medical wastes of 49.34% were mixed into domestic wastes or landfilled. Conclusion The management of medical wastes wasn't perfect in Changsha, which presented potential environmental pollution.

Concern on antibiotics contamination has been raised because of its serious environmental impact in recent years.The application of antibiotics,measurement method for antibiotics,mechanism of antibiotics degradation,and impact of antibiotics contamination on environment and human health were reviewed and commented.The advisable solution and future researches were prospected in this review,too.