Objective: To study the quenchable effect of Selenium to Luminol-H2O2-KMnO4 Chemiluminescence system and to establish a method for the determination of Selenium in serum.Methods: Chemiluminescence system was used to determine the Chemiluminogenic strength of Luminol-H2O2-KMnO4 and the detection of Selenium in serum was realized.Results:A detection limit with 6.5?10-4?g/ml and a 1.5?10-3～6.5?10-1?g/ml linear range were obtained.The average recovery fraction was 94.21% and the relative standard deviation was 2.97%.Conclusion: A satisfactory result for the determination of Selenium in serum by this method is obtained.

Objective:To establish a flow injection chemiluminescence determination method with micropipe-chip for speedy determination of uric acid in clinical samples.Methods:Basing on the rule that uric acid can greatly enhance the chemiluminescence emission intensity of K4Fe(CN)6-K3Fe(CN)6-luminol system,the prepared micropipe-chip was used to analyze the uric acid in serum under the selective conditions.Results:The enhancing chemiluminescence intensity was proportional to uric acid concentration over a range 2.0?10-5～1.0?10-2 g/L.The detection limit(3?) was 4.6?10-7 g/L.The relative standard deviation(RSD) was 3.09% and the recovery test results were 94.0%,99.7%,and 107.8%.Conclusion:The manufacture technique of micropipr-chip is simple but the proposed determination method of uric acid in human serum is sensitive,fast and convenient.

Purpose:To investigate the clinical characteristics, diagnosis and treatment of accessory breast tumors.Methods:Clinical and pathological data of 16 cases of accessory breast tumors were analyzed retrospectively. Results:There were 5 cases of accessory breast fibroadenoma, 2 cases of intraductal papilloma, 9 cases of accessory breast carcinoma. Local mass resection and radical resection were carried out respectively. All these patients had post operative chemotherapy and radiotherapy. 13 cases (81.3%0 were followed up for 1—10 year. As to results, 3 cases died of metastasis in 2,2.5 and 4 years respectively after operation;one case, reoperated because of local recurrence, still survives (over 3 years):and the others recovered well.Conclusions:It is possible for benign or malignant tumor to occur in the accessory breast,and the manifestation of the accessory breast tumors is similar to that of the primary breast tumor. Operation is the first choice for all treatment benign tumor with local mass resection including the whole accessory breast while accessory breast carcinoma should be treated by radical dissection.

Purpose: To investigate the diagnosis, pathological characteristics and treatment of primary malignant lymphoma of the breast (PMLB). Methods: Clinical and pathological data of 16 cases of PMLB were analyzed retrospectively. Results: No case was correctly diagnosed before operation. All the 12 cases were operated and identified by pathology, in which total mastectomy, radical mastectomy and modified radical mastectomy were carried out in 5, 3 and 4 patients respectively. Moreover, all cases were combined with post-operative chemotherapy or radiotherapy. 10 cases were followed up 1 - 10 years. 4 cases who survived 22 months on the average died of the tumor spread and metastasis during follows-up, and the others are still alive. Especially, 1 case, recurring 2 times locally, has survived for 4. 5 years after reoperation. Conclusions: The final diagnosis of this disease depends on the pathological examination. The majority of PMLB is non-Hodgkins lymphoma ( NHL), and mostly of B cell origin and are mostly related to the mucosa-associated lymphoid tissue ( MALT). Total mastectomy or mastectomy plus axillary nodes dissection is the preferred method of operation and the adequate post-operative chemotherapy is very important.

Enophthalmos ; etiology ; surgery ; Humans ; Orbital Fractures ; complications ; surgery ; Polyethylenes ; Prostheses and Implants ; Reconstructive Surgical Procedures ; methods ; Surgery, Computer-Assisted

This paper reports a method for direct determination of ascorbic acid by ultra-violet differential spectrometry and its application to analysis of ascorbic acid contents in vegetables, fruits and tablets. All the four (1st to 4th) derivatives of spectrometry were available for determination, and the better results were obtained from the 2nd derivative spectrometry. The results showed that this method was simple, rapid and accurate.

Arrhythmias, Cardiac ; pathology ; physiopathology ; Humans ; Purkinje Fibers

At the time of phage’s discovery, phage therapy was regarded as a possible treatment method against bacterial infection. Although phage therapy was used to treat and prevent bacterial infection in the former Soviet Union and Eastern Europe, it was abandoned by the West in the 1940s with the arrival of the antibiotic era. However, the ongoing evolution of bacterial multidrug-resistance has recently motivated the Western scientific community to reevaluate phage therapy for bacterial infections that are incurable by conventional chemotherapy. With the indepth study of phages, it’s increasingly acknowledged that phages, as the medicine to cure bacterial infection, are convenient, safe and efficient therapeutics. This paper summarizes the recent years’ advanced researches in this area.

Objective To investigate the concentration of blood uric acid and the prevalence of hyperuricemia in staff of a univer-sity so as to provide scientific basis for prevention and treatment of hyperuricemia.Methods Uric acid(UA)of 4 893 persons were detected by enzymatic method.For male whose UA>428 μmol/L,For female whose UA>357μmol/L were considered as hyperuri-cemia.People enrolled in the study were grouped according to age and analyzed.Results The average UA of males was (357.23± 75.17)μmol/L and was significantly higher than that of females(P <0.05).The average UA concentration and hyperuricemia rati-os in males of 20-<30,30-<40 years old groups were the highest,that of 60 -<70 group was the lowest,the difference was statistically significant(P <0.05).The average UA and hyperuricemia ratios of the male group decreased with age before 70 year old.But The average UA and hyperuricemia ratios of 70-<80 and ≥80 groups were higher than that of the 60-<70 group,the difference was statistically significant(P <0.05).The average UA and of hyperuricemia ratio of the female group increased with age.Conclusion The average UA concentration of males was significantly higher than that of female,in addition to that,hyperuri-cemia patients become younger and younger in male population.

Objective To study the resistance status of helicobacter pylori (HP) to commonly used antibacterial drugs as well as clinical effects of quadruple therapy in the patients with chronic obstructive pulmonary disease (COPD) infected by Hp.Methods A total of 180 cases of patients with Hp infection in the inpatient department and outpatient department of our hospital from August 2012 to December 2014 were collected,including 90 cases of COPD (COPD group) and 90 cases of non-COPD (control group).The gastric antrum mucosal tissues in all cases were taken for conducting Hp culture and identification.The resistance situation to clarithromycin,metronidazole,amoxicillin,levofloxacin and furazolidone in each group was observed.In addition,the two groups were equally divided into subgroup A,B and C according to different medication schemes:which were given 3 kinds of quadruple medication scheme containing amoxicillin and clarithromycin (group A),amoxicillin and levofloxacin (group B) and amoxicillin and furazolidone (group C).Then the clinical effects were compared.Results The resistance rate of infected Hp strains to clarithromycin in the control group was 21.11%;which to metronidazole was 55.56%;which to amoxicillin was 3.33%;which to levofloxacin was 5.56%,while which in the COPD group were in turn 26.67%,57.78%,7.78% and 16.67% respectively.No strain was found to be resistant to furazolidone in the two groups.There was statistically significant difference in the resistance to levofloxacin and double antibiotic resistance between the two groups (P<0.05).The eradication rates for using 3 kinds of quadruple therapy in 3 subgroups of control group were 90.0% in the group A,96.7% in the group B and 100.0% in the group C,while which in the COPD group were 86.7%,80.0% and 96.7% respectively;the Hp eradication rate for adopting the group B scheme had statistical difference between the two groups (P<0.05).Conclusion The resistance rate of COPD patient affected by Hp to levofloxacin has an increasing trend,so its use should be cautious.The quadruple scheme containing amoxicillin+furazolidone can serve as the preferred scheme for the initial Hp eradication therapy in COPD patients affected by Hp.