Objective:To evaluate the effect of NF-? B inhibitor-PDTC on TNF-? induced cytotoxicity in breast cancer cells.Methods:The cell survivals of breast cancer cell lines MCF-7 and MDA-MB-435s were measured by MTT assay in vitro.MDA-MB-435s cells were implanted into BALB/C mice. Animal tumor models were treated with TNF-?, PDTC and TNF-? plus PDTC.Results:The growth of two breast cancer cell lines was not inhibited by either TNF-?(2000U/mi or less)or PDTC.However,both breast cancer cells treated with TNF-?(2000U/mi)plus PDTC(50? mol)showed significant growth inhibition as compared with controls. In vivo, the growth of implanted tumor was significantly reduced in volume in mice treated with TNF-?(7.5?10 6U/kg,ip,bid) plus PDTC(50mg/kg,ip,bid) as compared with controls( P

G protein-coupled receptor 30(GPR30) was a novel estrogen receptor identified as membrane associated receptor in the late 1990s.This new member of estrogen receptors was independent of the classic nuclear estrogen receptor ? and ? due to the low homology and signifi cant difference between them.It was reported that GPR30 localized endoplasmic reticulum predominantly,which was expressed in diverse cancer cells and a wide range of systems throughout the body.The rapid non-genetic response,partially at least,transcription regulation of estrogenic effects were mediated by the novel receptor via transactivation of epidermal growth factor receptor and modulation of second messengers such as cyclic adenosine monophosphate(cAMP) and Ca2+.These pathways,possibly coordinate with ER?,were involved in various physiological,physiopathological and carcinogenesis process.Theoretically,GPR30 would be a novel therapeutic target in estrogen-related diseases such as breast carcinoma.

Objective To evaluate the clinical application of 3 D ultrasound in the diagnosis of bladder diseases. Methods In 21 patients with bladder disease,3 D images were studied with a Voluson 530D diagnostic system.At first,the two dimensional images of lesions were collected,then defined 3 D volumes of interest were stored,reconstructed and analyzed. Results 3 D ultrasound provided a clear stereoscopic view and depicted the appearance of the 21 bladder diseases,displayed the internal structures,and showed invasive depth within and outside the bladder wall and also metastasis of the adjacent organs. Conclusions 3 D ultrasound can clearly demonstrate the morphologic characteristics and internal structures of the bladder disease and provide much more reliable information for the diagnosis of diseases.

Objective To denoise digital radiographic images well.Methods A technique was presented that used the Anscombe's transformation to adjust the original image to a Gaussian noise model based upon the wavelet denoising method and the wavelet-domain Hidden Markov Tree(HMT) model.Wavelet domain HMT models were used to determine the dependencies of multiscale wavelet coefficients through the state probabilities of the wavelet coefficients,whose sedistribution densities could be approximated by Gaussian mixture model.Results The proposed method could keep natural images edges from damaging and increase PSNR.Conclusion Quantitative and qualitative DR images assessment shows that the proposed algorithm outperforms the traditional Gaussian filter in terms of noise reduction,quality of details and bone sharpness.

OBJECTIVE:To provide reference for rational use of opioids analgesics. METHODS:1 882 prescriptions of opi-oids analgesics for cancer pain collected from our hospital in 2014 were analyzed by defined daily dose(DDD)and drug utilization index(DUI). RESULTS:The diseases of opioids analgesics prescriptions for cancer pain in our hospital in 2014 were mainly lung cancer,accounting for 42.19%. Commonly used opioids analgesics included Morphine sulfate sustained-release tablets,Oxycodone Hydrochloride sustained-release tablets and so on,and their DUI were all below 1.0. Top one drug in the list of amount and con-sumption sum was Morphine sulfate sustained-release tablets,and its main dosage form were tablets,injection and patches,in which tablets occupied the largest proportion,reaching 97.30%. CONCLUSIONS:The application of opioids analgesics for cancer pain in our hospital is basically rational in terms of drug types,dosage form and route of administration,but the dose of opioids an-algesics is small and their DUI is lower than 1;at the same time,there are a few irrational prescription.

Objective To explore the expression and activation of nuclear transcription factor ?B(NF ?B) in human breast cancer (BC), and the relationship between the activity of NF ?B and malignant potential of BC. Methods The protein expression of NF ?B p65 in BC tissue and paratumor tissue were measured by Western blot; NF ?B DNA binding activity was examined by electrophoretic motility shift assay(EMSA)in 26 BC tissues and 12 paratumor tissues of BC.Results The levels of NF ?B p65 protein and NF ?B DNA binding activity in the tumor tissue were higher than those in the paratumor tissues(P

Combined Modality Therapy ; Consensus ; Head and Neck Neoplasms ; therapy ; Humans

Disease Outbreaks ; Humans ; Influenza A Virus, H1N1 Subtype ; Influenza, Human ; epidemiology ; virology

Objective To study expression enhancement and significance of Y14 and Upf1 in human breast cancer cell lines and tissue. Methods Immuocytochemistry and laser scanning confocal microscope(LSCM) were applied. Y14 and Upf1 were determined in human breast cancer cell lines(MCF-7,ZR-75-30,T47D,MDA-MB435s,MDA-MB-453, MDA-MB-231) and breast epithelial cell line ( HBL-100). Results (1) Y14 and Upf1 level of breast cancer cells are obviously higher than that in breast epithelial cell line (P < 0. 05 ). (2)Y14 and Upf1 level of MDA-MB-231 are obviously higher than that in MCF-7. (3)The expression enhancement of Y14 and Upf1 level are obviously higher in human breast cancer tissue. Conclusion The expression level of Y14 and Upf1 in breast cancer cells and tissue enhance obviously.

Objective To evaluate the roles of signal transducer and activator of transcription 3(STAT3) played in delayed xenograft rejection.Method Mice-to-rats cardiac xenograft model was established and recipients were administrated by inhibitor of phosphorylation of STAT3,5,15-Diphenylporphyrin(DPP),and immunosuppressive agent,ciclosporin A (CsA) alone or both.The survival of graft was analyzed.Immunohistochemistry and immunoblotting were utilized to detect the phosphorylated STAT3 (p-STAT3),and real-time polymerase chain reaction was used to assess STAT3-targeted genes in grafts.Result The expression of p-STAT3 was increased significantly with the prolonged survival in grafts (P ＜ 0.05).Administration of DPP and CsA both significantly prolonged survival of the grafts (P＜0.05),and decreased the expressions of STAT3-targeted genes including Bcl-xl,Bcl-2,Cyclin D1,C-myc and VEGF (P＜0.05).DPP and CsA exerted the synergistic effects.Conclusion STAT3,which is persistently activated in cardiac xenografts,probably up-regulates downstream genes to promote proliferation and to inhibit apoptosis of endothelial cells,leading to the activation of endothelial cells and delayed xenograft rejection.