Objective: To evaluate the osteogenic activities of Bio-oss after combining with fibrin glue in reconstruction of canine mandibular defects. Methods: The second and fourth premolar teeth and the second molar teeth were extracted bilaterally, in 9 hibrid canines, resulting in 6 bone defects (1 cm × 1 cm) in each canine. Bio-oss, Bio-oss+FG and FG were implanted into the bone defects of the second, fourth premolar teeth and the second molar teeth, respectively. Canines were executed in group of 3 after 4, 8, and 12 weeks to observe the healing of soft tissues. The bone density was assessed by X-ray, the property of Bio-oss were observed via gross specimen, and the morphology of the newly-formed bone was observed by tissue sections. The proportion of newly-formed bone was obtained by computer image analysis (SAS software, analysis of variance). Results: Stage I healing of soft tissues was achieved in all animals. The bone densities were not significantly different between Bio-oss+FG and Bio-oss groups. The bone in FG group had transparent area. We also found that the bone in Bio-oss+FG group was closely combined and there were sccatered Bio-oss dusts in the soft tissues of the Bio-oss group. The newly-formed bone in the FG group was only found in the border between the defects and FG. The proportion of newly-formed bone was less in the Bio-oss+FG group than in the Bio-oss group at 4, 8, and 12 weeks after extraction(P<0.05). Conclusion: Fibrin glue can well shape the Bio-oss and has satisfactory biocompatibility, but it reduces the new bone forming.

Objective To study the analgesic effect of sufentanil combined with Dezocine in endoscopic resection of fallopian.MethodsA total of 188 patients with tubal pregnancy from April 2013 to December 2014 in Haining central hospital were enrolled in the study, they were randomly divided into two groups (n=94).On the basis of conventional intravenous analgesia, the patients in control group was were received sufentan, the observation group were given sufentan combined with dezocine.The score of pain at different time points after operation, the Ramsay sedation score, and the level of IL-6 and IL-10 were compared.The adverse reactions were compared between the two groups.ResultsThe score of VAS in the observation group was significantly lower than that in the control at 4h, 8h, 12h and 24h after operation (P<0.05).The score of VAS in the two groups were similar at 48h after operation;the Ramsay score in the observation group was significantly higher than that in the control group(P<0.05) at five time points (4h, 8h, 12h, 24h and 48h after operation);when compared Compared with the indexes before operation, the level of IL-6 was significantly increased in the two groups at 24h and 48h, but the observation group increased less, the IL-6 in observation group was lower than that in the control group at the two points(P<0.05);while the IL-10 was higher than that beforeoperation, and the observation group was more higher(P<0.05).The adverse reactions in the two groups were mainly dizziness, nausea, pruritus and lethargy, and the incidence was only 12.8% in the observation group, which lower than that in the control of 29.8%(P<0.05).ConclusionIt which dazocine combined with sufentanil had a good analgesic effect in the process of analgesia in gynecological laparoscopic resection of fallopian tube after the operation.The VAS score and Ramsay sedation score was improved significantly.The levels of inflammatory factors were lower, and the incidence of adverse reaction was significantly reduced.It is a reliable method for clinical intravenous analgesia.

Objective:To evaluate the osteogenic activities of Bio-oss after combining with fibrin glue in reconstruction of canine mandibular defects.Methods:The second and fourth premolar teeth and the second molar teeth were extracted bilaterally, in 9 hibrid canines,resulting in 6 bone defects(1 cm?1 cm)in each canine.Bio-oss,Bio-oss+FG and FG were implanted into the bone defects of the second,fourth premolar teeth and the second molar teeth,respectively.Canines were executed in group of 3 after 4,8,and 12 weeks to observe the healing of soft tissues.The bone density was assessed by X-ray,the property of Bio-oss were observed via gross specimen,and the morphology of the newly-formed bone was observed by tissue sections.The proportion of newly-formed bone was obtained by computer image analysis(SAS software,analysis of variance).Results:StageⅠhealing of soft tissues was achieved in all animals.The bone densities were not significantly different between Bio-oss+FG and Bio-oss groups.The bone in FG group had transparent area.We also found that the bone in Bio-oss+FG group was closely combined and there were sccatered Bio-oss dusts in the soft tissues of the Bio-oss group.The newly-formed bone in the FG group was only found in the border between the defects and FG.The proportion of newly-formed bone was less in the Bio-oss+ FG group than in the Bio-oss group at 4,8,and 12 weeks after extraction(P

Objective To study the protective role against cisplatin-induced ototoxicity by using radix astra-gali in rats. Methods Forty rats were randomly divided into four groups. Group A received saline as controls. Group B received radix astragali. Group C received injection of cisplatin(4 mg/kg) as experiments for 6 days. Group D received both radix astragali (5 g/kg) and cisplatin (4 mg/kg). Distortion product acoustic emission (DPOAE) was applied to each rat before and 7 days after cisplatin injection. All the animals were sacrificed on the 7th day. Half of the cochleas were observed by frozen section and the apoptosis of hair cells was detected by TUNEL meth-od. Scanning electron microscopy was utilized to evaluate the cochlear morphology of the other rats. Results The DPOAE amplitudes of Group C decreased significantly compared to the group A and group B(P<0.01). The dam-age and apoptosis of hair cells were noted in the group C and group D, while the hair cells of group A and group B showed no sign of apoptosis or damage. Compare to experimental group , the DPOAE amplitudes of group D were higher, and the damage and apoptosis of hair cells were significantly lower(P<0.05). Conclusion This study sug-gests that radix astragali can effectively reduce cisp[atin ototoxicity.

Protein tyrosine kinase genes are the largest family of oncogenes. This is not surprising since protein tyrosine kinases are important components of signal transduction pathways that control cell shapes, proliferation, differentiation, and migration. This review will address the recent progress in understanding the function of Eph receptor and ephrin ligands in normal development and how disregulation of these functions could promote tumorigenesis.

Objective To establish a method for the cultivation of hain follicle outer root sheath (ORS) cells of murine vibrissa and to study their expression of uPA/uPAR. Methods The ORS cells of murine vibrissa were cultured by combination of enzyme digestion and tissue culture with the feeder layer of dermal sheath cells of the hair follicle. The cells and their uPA/uPAR expression were identified by immunocytochemistry (ICC). Results The feeder layer of DS was suitable for the culture of ORS cells. ORS cells expressed uPA/uPAR protein. Conclusion The DS of hair follicle is a better feeder layer for the growth of ORS cells. ORS cells have the ability to migrate and proliferate.

Objective To compare the efficacy of retrograde cardioplegia for myocardial perfusion with that of antegrade cardioplegia at the same flow rate using magnetic resonance techniques. Methods Myocardial perfusion was evaluated with magnetic resonance imaging in isolated pig hearts during antegrade and retrograde cardioplegia. Phosphorus 31 magnetic resonance spectroscopy was used to determine the efficacies of both cardioplegic techniques in sustaining myocardial energy metabolism. Results Magnetic resonance images showed that contrast-enhanced signal-time courses obtained from different regions of the myocardium during retrograde cardioplegia varied significantly, whereas signal-time courses acquired during antegrade cardioplegia appeared more uniform. Localized phosphorus 31 spectra showed that retrograde cardioplegia resulted in myocardial ischemia at the flow rate of 10 ml/min. However, no significant changes were observed in myocardial energy metabolites until the flow rate was decreased to 3 ml/min during antegrade cardioplegia. Conclusion Microvascular perfusion provided by retrograde cardioplegia varies more significantly among various small areas of the myocardium than that did by antegrade cardioplegia. Moreover, the efficacy of retrograde cardioplegia in sustaining normal myocardial energy metabolism is lower than that of antegrade cardioplegia.

Objective To isolate and culture the hair follicle Bulge cells of rats and study their morphological, immunological characteristics. Methods Hair follicle Bulge obtained by micromanipulation and enzyme digestion was cultured in intro. The morphological features and numbers of Bulge cells were identified and counted with light microscopy. Immunocytochemical staining was used to detect expressing of K19 and ?_ 1integrin in cultured cells. Results Bulge cells after enzyme digestion pasted rapidly. Cells were small and round, paving stone-shape, and had a large ratio of nuclear to cytoplasm. The characteristics suggested a primitive morphologic feature. Cells growth well and could maintain low differentiation and higher reproductive activity, they co-expressing K19 and ?_ 1integrin, immunofluorescence staining showed the cells expressed ?6-integrin strongly, weakly or no expressing CD71. Conclusion This culture system can amplify a lot of pure hair follicle Bulge cells in short time. We successfully isolate and culture the hair follicle Bulge cells of rats in intro and can keeping its property for a long time.

Objective To investigate the diagnostic value of serum tumors M2 pyruvate kinase (TuM2-PK),carcino-embryonic antigen (CEA),CA199,CA724,CA125 and CA242 in colon cancer.Methods Serum levels of TuM2-PK,CEA,CA199,CA724,CA125 and CA242 in 231 patients with colon cancer,105 patients with colon benign lesion diseases and 166 normal controls were measured by the ELISA and electrochemiluminescence assays.The operation working characteristic curve (ROC) and the logistic regression-ROC were used to evaluate the diagnostic value of tumor markers for colon cancer individually and in combination.The models of logistic regression analysis and partial least-squares discriminant analysis (PLS-DA) were established to diagnose patients with colon cancer based on the optimal panel of serum tumor markers.Resalts Concentrations of serum TuM2-PK,CEA,CA199,CA125 and CA724 in the colon cancer group are higher than those in the colon benign diseases group and the normal controls (P＜0.05).The area under the operation working characteristic curve (AUC) of CA199 is the highest 0.79 [95％ confidence interval (95％CI),0.75-0.83] at the cutoff value of 69.5 U/L with 64.1％ of sensitivity and 89.7％ of specificity.The AUC of combined serum tumor markers based on logistic regression analysis is higher than those in individuals,of which serum (CEA+ CA199+TuM2-PK) is the optimal [AUC=0.89,95％ confidence interval(95％CI),0.86-0.92].The diagnostic accuracy of logistic analysis and PLS-DA model for colon cancer is 82.7％ and 77.5％,and for colon cancer is 93.7％ and 95.6％,respectively.Conclusion The combination of serum tumor markers CEA,CA199 and TuM2-PK is more suitable as a diagnostic model for the screening of colon cancer.

Objective To discuss the indications and complications of primary closure of bile duct incision in laparoscopic bile duct exploration and balloon dilatation catheter dilatation to treat the papillary stenosis and the intrahepatic bile duct stenosis. Methods A pospective study of 42 ptients of bile duct incision closure primary in laparoscopic bile duct exploration and balloon dilatation catheter dilatation, laparoscopic bile duct exploration and extraction of bile duct stones with choledochotomy was first adopted in order to clear the stones, then followed by the balloon dilatation catheter(explosive pressure reached 2020 kPa, used 505kPa) to dilate the papillary stenosis and the intrahepatic bile duct stenosis (CT-7542～ CT-75104) until the stenosis was released. Whether the primary closure of duct incision was selected or not, it was based on the situation of intraoperative choledochoscopic exploration, if it had been selected, the closure of bile duct incision would accepted by using absorbable suture 4-0 or 5-0, without placing bile duct drainage.It was routinely to place the drainage tube in the oriffice of the lesser omentum. Results 41 out of 42 patients had obtained successful duct clearance, the dilatation of the stenosis to reach the expected expansion and without bile leakage. One patient had bile leakage about 30-150 ml daily persisted for 4 days through cured conservatively. Conclusion Eventually it was safe and effective for some patients who had completed successful duct clearance and the dilatation of the stenosis to reach the expected expansion with the balloon dilatation catheter. They were adopted to the primary closure of duct incision using absorbable suture and did not need to place bile duct drainage.