Objective To investigate the expression of an inhibitor gene of apoptosis, clusterin, in prostate cancer and its relationship with the genesis and progression of prostate cancer and with the expressions of bcl 2 and p53. Methods The expressions of clusterin, bcl 2, and p53 in 10 cases of normal prostatic tissues, 15 cases of benign prostatic hyperplasia (BPH), and 49 cases of prostate cancer were examined by immunohistochemical staining. Results The rates of strong positive or weak positive of clusterin in normal prostatic tissues, BPH, and prostate cancer were 10% (1/10), 66.6% (10/15), and 91.8% (45/49), respectively. The expression level of clusterin in prostate cancer tissues was significantly higher than that in normal prostatic tissues ( P

The article summarizes the questions of clinical teaching of advanced urologists,and find out some measures to improve the clinical teaching quality of advanced urologists.

Total hip replacement (THR) is replacing the prosthesis stem similar to human bone that takes advantage of the material with both good mechanical properties and biocompatibility to the damaged articular surface. Thus it can not only alleviate or even eliminate the pain but also effectively maintain the joint stability and freedom and restore its normal performance. Finite element analysis was used in this study to establish a 3D model of artificial hip stem, and explore its fatigue properties of different materials to ensure the safety and reliability. The calculating obtained two results of different metal hip prosthesis, including lifetime and deformation. The minimum service life of titanium prosthesis reaches 568 million times, which satisfies ISO standards, while the stainless steel does not suit to be a prosthesis material.
Arthroplasty, Replacement, Hip ; Finite Element Analysis ; Hip Joint ; Hip Prosthesis ; Humans ; Materials Testing ; Models, Anatomic ; Prosthesis Failure ; Reproducibility of Results ; Stainless Steel ; Titanium

OBJECTIVE:To investigate the inhibitory effect of the total flavoniods of Ampelopsis grossedentata on human hepatoma carcinoma cells(HCC) in vitro.METHODS:The inhibitory effect of the total flavoniods of Ampelopsis grossedentata extract on human hepatoma carcinoma BEL7404 cells in vitro was analyzed by MTT,cell growth curve method and colony formation method,respectively.RESULTS:The total flavonoids of Ampelopsis grossedentata significantly inhibited the growth of BEL 7404 cells in vitro.MTT assay showed that the IC50 was 28.59 ?g?mL-1;the cell growth curve measurement showed that the total flavonoids of Ampelopsis grossedentata significantly inhibited the growth of BEL 7404 cells;the colony formation assay showed that the IC50 was 100% at a drug concentration of above 22.22 ?g?mL-1 and the IC50 was 58.05% at a drug concentration of 14.81 ?g?mL-1.CONCLUSION:The total flavonoids of Ampelopsis grossedentata have a remarkable inhibitory effects on the growth of BEL 7404 in vitro.

OBJECTIVE:To study the regulatory effect of lentinan (LTN) on immune function in ulcerative colitis (UC) rats and its mechanism. METHODS:64 SD rats were randomly divided into normal group(12 rats)and modeling group(52 rats). UC model was induced in modeling group by a compound method of 2,4-dinitrochlorobenzene(DNCB)acetone and 2,4-DNCB etha-nol. After modeling, rats were randomly divided into model group, positive group (sulfasalazine tablet, 300 mg/kg), LTN low-dose,medium-dose and high-dose groups(0.2,0.4,0.8 mg/kg),with 10 rats in each group. Treatment groups were given rele-vant medicine intragastrically,and normal group and model group were given normal saline intragastrically,once a day,for consec-utive 15 d. The levels of CD3+,CD4+,CD8+in peripheral blood lymphocytes(PBMC)and the levels of TNF-α,IL-2,IL-4 and IL-10 in colon were all determined,and the pathological changes of colon tissue were observed. RESULTS:Compared with normal group,the levels of CD3+,CD4+,CD8+,the ratio of CD4+/CD8+,and the levels of IL-4 and IL-10 decreased significantly,while the levels of TNF-α and IL-2 in colon tissue significantly increased(P<0.05);obvious ulcerative lesion was observed in colon tissue (concentrated in grade Ⅱ and Ⅲ). Compared with model group,the levels of CD3+,CD4+,CD8+,the ratio of CD4+/CD8+,and the levels of IL-4,IL-10 increased significantly,while the levels of TNF-α,IL-2 in colon tissue significantly decreased (P<0.05);the inflammation of colon tissue was significantly reduced,and the ulcer formation was significantly improved. CONCLUSIONS:LTN has a certain improvement effect on UC model rats,and its mechanism may be related to enhancing the immune function.

Functional dizziness is one of the most frequent functional disorders in adult patients.The termfunctional dizziness is not a nosological entity,but comprises several diagnoses.This study is aim to summarize the typical symptoms of functional dizziness and signs and symptoms of non-functional dizziness.

Objective To study the surgical repair mode of skin cancer in facies in aged patients. Methods The modes of surgical repair in 216 cases of aged patients with skin cancer in facies were analyzed. Results The special characteristics of physiology and pathology and the facies anatomy in the elderly determined the complexity of surgical repair. The surgical mode of excision and suture was the safest and the most efficacious. Skin flap transfer could mostly satisfy the function requirements of facies for the wound that was not able to be sutured after excision. Free skingrafting was applicable to the case that the deep tissue was not invaded by the tumor and the general condition of the patients was not much good. Conclusion The order of surgical repair modes for skin cancer in the facies in the elderly is excision and suture, skin flap transfer, and free skingrafting. Local skin flap must be the primary source as the selection of the skin flap is concerned. The suitable axial pattern skin flap or musculo-cutaneous flap can be selected according to the different section of the facies while there is no suitable local tissue.

Objective To analyze proliferating cell nuclear antigen(PCNA) index in invasive, proliferative,aged parts of keloid to explore the mechanism of keloid invasive growth characteristics. Methods PCNA in 10 cases of keloid and 10 cases of normal skin was detected by immunohistochemical staining, and the PCNA index of different parts in keloid and normal skin was compared. Results The PCNA index of invasive part in keloid was obviously higher than that of proliferative,aged parts of keloid and normal skin (P

Aged ; Endocarditis ; etiology ; therapy ; Humans ; Kidney Transplantation ; adverse effects ; Male

Medical clinical education is really the beginning of clinical work,and it should be reformed accordingly based on actual changes in health care.This article discusses that the clinical medical education should be reformed to face serious medical problems.