Objective To investigate the expression of an inhibitor gene of apoptosis, clusterin, in prostate cancer and its relationship with the genesis and progression of prostate cancer and with the expressions of bcl 2 and p53. Methods The expressions of clusterin, bcl 2, and p53 in 10 cases of normal prostatic tissues, 15 cases of benign prostatic hyperplasia (BPH), and 49 cases of prostate cancer were examined by immunohistochemical staining. Results The rates of strong positive or weak positive of clusterin in normal prostatic tissues, BPH, and prostate cancer were 10% (1/10), 66.6% (10/15), and 91.8% (45/49), respectively. The expression level of clusterin in prostate cancer tissues was significantly higher than that in normal prostatic tissues ( P

The article summarizes the questions of clinical teaching of advanced urologists,and find out some measures to improve the clinical teaching quality of advanced urologists.

Aged ; Endocarditis ; etiology ; therapy ; Humans ; Kidney Transplantation ; adverse effects ; Male

Total hip replacement (THR) is replacing the prosthesis stem similar to human bone that takes advantage of the material with both good mechanical properties and biocompatibility to the damaged articular surface. Thus it can not only alleviate or even eliminate the pain but also effectively maintain the joint stability and freedom and restore its normal performance. Finite element analysis was used in this study to establish a 3D model of artificial hip stem, and explore its fatigue properties of different materials to ensure the safety and reliability. The calculating obtained two results of different metal hip prosthesis, including lifetime and deformation. The minimum service life of titanium prosthesis reaches 568 million times, which satisfies ISO standards, while the stainless steel does not suit to be a prosthesis material.
Arthroplasty, Replacement, Hip ; Finite Element Analysis ; Hip Joint ; Hip Prosthesis ; Humans ; Materials Testing ; Models, Anatomic ; Prosthesis Failure ; Reproducibility of Results ; Stainless Steel ; Titanium

Objective To investigated the effect of somatostatin(SS)on cholecystokinin-induced alterations of exocrine function and the redistribution of the F-actin in the pancreatic acinar cells.MethodsIn vitro,isolated rat pancreatic acini were divided into 12 groups:normal control group,different concentrations ofcholecystokinin-octapeptide groups(CCK-8)of 10-12,10-11,10-10,10-9 or 10-8 mol/L,SS group(10-7mol/L),SS and different dose CCK-8 groups.The acinar cells were stimulated with different concentrations of CCK-8 for 30 min in the presence or absence of 10-7 mol/L SS.Amylase and trypsin activity were measured using corresponding assay kits,and F-actin distribution in pancreatic acinar cells were detected by laser confocal microscopy.ResultsSS stabilized the structure of cytoskeleton in acinar cells,and remarkably increased the ratio of subapical/basolateral F-actin(P

Objective To investigate the clinical characteristics,diagnosis and treatment of invasive pituitary adenoma.Methods The clinical presentations,imaging studies and surgical outcomes in 62 patients with invasive pituitary adenomas and 78 patients with noninvasive pituitary adenomas were analyzed retrospectively.Results Invasive pituitary adenomas were more common in patients with macroadenomas than those with microadenomas(P

Objective To construcr a phage display library of human single-chain Fv antibodies from peripheral blood lymphocytes of people with positive serum antibody against hepatitis B virus core protein. Methods The heavy chain and light chain variable region gene of human immunoglobulin were amplified individually by RT-PCR from peripheral blood Lymphocytes mRNA of patients with positive serum hepatitis B virus core protein antibody and randomly combined through a DNA linker encoding the peptide (Gly 4Ser) 3 to construct single-chain variable fragments (ScFv)gene. In the presence of helper phage M13KO7, the ScFv were displayed on the surface of recombinant phages. Results Repertoire antibody library was obtained with 10 6 clonies resistant to ampicillin and kanamycin. Conclusions A typical phage display library of human single-chain Fv antibodies has been constructed Using RT-polymerase chain reaction (RT-PCR) and phage display technique, human single-chain Fv antibodies can be screened from this library.

Medical clinical education is really the beginning of clinical work,and it should be reformed accordingly based on actual changes in health care.This article discusses that the clinical medical education should be reformed to face serious medical problems.

OBJECTIVE:To study the regulatory effect of lentinan (LTN) on immune function in ulcerative colitis (UC) rats and its mechanism. METHODS:64 SD rats were randomly divided into normal group(12 rats)and modeling group(52 rats). UC model was induced in modeling group by a compound method of 2,4-dinitrochlorobenzene(DNCB)acetone and 2,4-DNCB etha-nol. After modeling, rats were randomly divided into model group, positive group (sulfasalazine tablet, 300 mg/kg), LTN low-dose,medium-dose and high-dose groups(0.2,0.4,0.8 mg/kg),with 10 rats in each group. Treatment groups were given rele-vant medicine intragastrically,and normal group and model group were given normal saline intragastrically,once a day,for consec-utive 15 d. The levels of CD3+,CD4+,CD8+in peripheral blood lymphocytes(PBMC)and the levels of TNF-α,IL-2,IL-4 and IL-10 in colon were all determined,and the pathological changes of colon tissue were observed. RESULTS:Compared with normal group,the levels of CD3+,CD4+,CD8+,the ratio of CD4+/CD8+,and the levels of IL-4 and IL-10 decreased significantly,while the levels of TNF-α and IL-2 in colon tissue significantly increased(P<0.05);obvious ulcerative lesion was observed in colon tissue (concentrated in grade Ⅱ and Ⅲ). Compared with model group,the levels of CD3+,CD4+,CD8+,the ratio of CD4+/CD8+,and the levels of IL-4,IL-10 increased significantly,while the levels of TNF-α,IL-2 in colon tissue significantly decreased (P<0.05);the inflammation of colon tissue was significantly reduced,and the ulcer formation was significantly improved. CONCLUSIONS:LTN has a certain improvement effect on UC model rats,and its mechanism may be related to enhancing the immune function.

OBJECTIVE:To introduce positive response to the security and effectiveness issues on OTC cough and cold medicines for children in New Zealand in order to provide reference for drug risk-benefit evaluation and putting forward reasonable management measures in China. METHODS:The concept of OTC cough and cold medicines for children were introduced firstly. Then the evaluation and countermeasures of OTC cough and cold medicines for children in New Zealand were also introduced. RESULTS & CONCLUSIONS:It has significance for the evaluation of drugs for sale that are review and evaluation,study and countermea-sures and dynamic tracking for OTC cough and cold medicines for children in New Zealand and the realistic attitude of New Zealand Drug and Medical Instruments Security Bureau.