1.EFFECT OF HEMOPERFUSION WITH IMMOBILIZED SPECIFIC ABSORBENT ON SEPSIS IN RATS
Yang SHAO ; Xiang WANG ; Gang FENG
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
To observe the changes in hemorheology, endotoxin and TNF ? in blood after hemoperfusion(HP) with adsorbent immobilized polymyxin B (PMB) on sepsis in rats. Wistar rats were randomly divided into three groups:LPS+HP+PMB,LPS+HP and LPS. All the rats received intravenously injection of lipopolysaccharide (LPS, Escherichia coil O111:B4,1mg/kg). Plasma of the rats in the group of LPS+HP+PMB was passed through a column containing sepharose coated activated charcoal immobilized polymyxin B at the 4th hour after LPS injection. The treated plasma was transfused bak after being mixed with blood cells. In LPS+HP group, the column did not contain immobilized polymyxin B. The animals of LPS group received LPS only. Quantitative endotoxin determination in blood was done with limulus amebocyte lysate test,TNF ? of the plasma assayed with ELISA, and hemorheology parameters were also observed after hemoperfusion. In LPS+HP+PMB group, the concentration of plasma was significantly decreased after hemoperfusion, but it was still significantly higher than the baseline value, and there was a decrease of blood cell ratio after hemoperfusion. The results suggest that specific adsorbent could remove endotoxin in the circulation and improve hemorheology.
2.Application of swept-source optical coherence tomography in ophthalmology
Shuangshuang ZHOU ; Gang TAN ; Yi SHAO
Recent Advances in Ophthalmology 2017;37(8):788-792
Accurate qualitative and quantitative assessment of anterior or posterior segment structures is crucial,not only for proper diagnosis of different ocular diseases but also for optimal surgical planning and postoperative follow-up.As a noninvasive and noncontact method,optical coherence tomography (OCT) has become an essential tests for the diagnosis of retinal diseases.To a more recent technology,swept-source OCT (SS-OCT) improved the depth of imaging and the scan speed,which can perform anterior segment evaluation liking corneal graft examination.Meanwhile,this newly technology add multimodal approach such as EVV,OCTA and En face OCT,for angiography and choroid mapping.Thus,SS-OCT has become a valuable imaging strategy to understand the pathologies of retinal diseases such as PVD,diabetic maculopathy,neovascularization and macular telangiectasia.With the use of multimodal approach,SS-OCT is beneficial for optimal surgical planning and postoperative follow-up for the vitreoretinal diseases,such as epiretinal membrane,retinal detachment,and diabetic taction retinal detachment.Moreover,this technology can help us better understand the ophthalmic pathologies of glaucoma and tumors.This article reviews the technical principles of SS-OCT and the advances in clinical applications.
3.The Influence of anterior crossbite on the position and morphology of teeth
Gang WU ; Jinling SHAO ; Yan HE
Journal of Practical Stomatology 2001;0(03):-
Objective: To study the influence of anterior crossbite on the position and morphology of teeth. Methods: 81 teenagers with permanent dentition and anterior crossbite were in volved in group 1 and 95 teenagers in permanent dentition without anterior crossbite in group 2. The teeth in both groups were compared on their position and morphology. Results:① The percentage of ill-positioned teeth in maxilla in group 1 and in group 2 was 14.0% and 8.8% respectively (P
4.Advancement of breast cancer and angiogenesis
Hecheng LI ; Gang LIU ; Zhimin SHAO
China Oncology 1998;0(01):-
Angiogensis plays an important role in tumor growth and metastasis,and anti-angiogenic therapy is a promising treatment of cancer. We review the proceedings on angiogensis and breast cancer ,and the progress in the field of anti-angiogenic therapy for breast cancer.
5.Application of Leucocyte Adherence Inhibition Test in Determining Immunological Activity of Ribonucleic Acid
Wang CAN ; Shao HONG ; Chen GANG
China Pharmacist 2015;(11):1848-1850
Objective:To establish a method for determining the immunological activity of ribonucleic acid. Methods: Leucocyte adherence inhibition test ( LAI) was applied, and the important parameters of LAI including the mouse strain, drug concentration, treatment time, content of buffer solution and cell density were researched. The immunological activity of RNAⅠ, Ⅱand Ⅲ was re-spectively determined by the method. Results:Stable and reliable parameters were obtained: the sample concentration was 10 mg· ml-1 , the treatment time was 2 hours, Ca2+ and Mg2+ were necessary for the buffer solution, and the cell density was about 4 × 107 cell·ml-1 . The strain of mouse showed no effect on the results. As a result, the determination method for immunological activity was established. Using the method, the immunological activity of RNA Ⅰ,Ⅱand Ⅲ was determined 3 times, and the results met the re-quirements with RSD below 20%. Conclusion:The method is suitable for determining the immunological activity of RNA.
6.A 4-year follow-up of functional rehabilitation in a patient with allografted forearms
Zheng-Gang BI ; Ming SHAO ; Qing-Yang GUO ;
Chinese Journal of Orthopaedic Trauma 2004;0(12):-
Objective To report the functional reconstruction and rehabilitation for a patient who under- went allograft for both of his forearms and hands.Methods One male patient underwent allograft for both of his forearms and hands in October 2002 in our department to reconstruct his hand functions.The allografted hands were intervened with an integrated rehabilitation program,which involved administration of immunosuppressants,post- operative monitoring,postoperative functional training,massage,physiotherapy,orthosis,performance training, sensation training,secondary operation and mental rehabilitation.The patient was followed up for 4 years.Results The forearms and hands of the patient were in good shape and regained nearly normal sensation.The distance of two-point-discrimination was 2.5 cm to 4.0cm.The TAM (total active motion) of fingers was fine.The patient could look after himself well and were healthy in psychology.Conclusion An integrated rehabilitation program can yield satisfactory results in the management of allografted forearms and hands.
7.A Secretive Pichia pastoris Expression Vector for Direct PCR Product Cloning
Chao ZHANG ; Gang LIU ; Shao-Wen YU ; Miao XING ;
China Biotechnology 2006;0(01):-
A secretive expression vector of Pichia pastoris system which can be used for the direct cloning of PCR products was constructed,and was verified through the expression of recombinant cellobiohydrolase II in Pichia pastoris.A randomly selected fragment was amplified with properly designed primers by PCR.The XhoI and Eam1105Ⅰ restriction sites were included in the 5'end of the fragment,and the Eam1105Ⅰ and XbaI restriction sites were included in its 3'end.The PCR amplified product was inserted into the P.pastoris expression plasmid pPICZ?A through XhoI and XbaI restriction sites and the resultant plasmid was digested with Eam1105Ⅰ,and lastly the big fragment was recovered,generating the P.pastoris expressive Tvector pPICZ?T.Then the cellobiohydrolase II of T.reesei was successfully expressed in P.pastoris with this expressive Tvector.Such results indicated that the constructed expression Tvector was convenient for PCR product cloning,and was effective for heterologous protein expression in P.pastoris.On the other hand,the application of the expression Tvector avoided the introduction of additional amino acids at the Nterminus of the expressed protein,which generally occurred when normal expression vectors were used in secretive expression system.
8.Advances in the research of the effects of tea polyphenols in cancer prevention and treatment
Gang LIU ; Jinsong LU ; Zhimin SHAO ; Al ET
China Oncology 2001;0(03):-
The main component of the tea is tea polyphenols, which can inhibit the processes of carcinogenesis and development of a variety of human cancers by diverse mechanisms. Further research may provide us with more information about the anticancer activities of these promising compounds and facilitate the usage in cancer prevention and treatment.
9.Blood pressure is associated with body fat and insulin resistance in non-hypertensive population
Zeyuan LU ; Yihao LIN ; Hao SHAO ; Gang ZOU
Chinese Journal of Endocrinology and Metabolism 1985;0(01):-
Relationship between blood pressure and insulin resistance was analyzed in 839 non hypertensive subjects (fasting plasma glucose
10.Study on blood lipids of patients with ischemic cerebral vascular disease
Cheng-Guo ZHANG ; Jian XIE ; Yan SHAO ; Yi-Gang XING ;
Chinese Journal of Neurology 2001;0(01):-
Objective To study blood lipids levels of patients with ischemic cerebral vascular disease (ICVD).Methods The blood lipids levels were measured in 2886 subjects with ICVD enrolled from 1991 to 2004,in which 1430 subjects from 2000 to 2004 were classified into a sub-group;400 healthy persons receiving physical examination were enrolled as controls.Results The blood TC,TG and LDL-C levels were significantly higher in the sub-group than those in the control group.Both in males and females, the morbidity of abnormal blood lipid metabolism and blood lipids level gradually increased with the age and the lapse of decades.Total cholesterol level of male younger than fifty,averaging (4.43?0.51) mmol/L, increased to (4.96?0.85) mmol/L after fifty years old.The same thing happened to females,from (4.30?0.49) mmol/L to (5.01?0.90) mmol/L.TG and LDL-C increased in patients older than fifty compared with those younger than fifty.In the groups above 50 years old,the blood lipids levels of females were significantly higher than those of males.The morbidity of abnormal blood lipid metabolism of male increased from 24.5% in (1991 to 1994) to 38.1% (2001 to 2004) and of female from 22.3% (1991 to 1994) to 38.5% (2001 to 2004).Conclusions Abnormal blood lipid in Foshan is one of the most important reasons to ICVD.