Objective: To establish the model of inbred strain rat heart heterotopic transplantation.Methods:Male Lewis rats were used as recipients and Brown-Norway rats as donors,and the heterotopic transplants were done using ono's method with some modifications.The ascending aorta and pulmonary artery of donor were anastomosed end-to-side to the recipient abdominal aorta and inferior vena cava respectively.Results:48 heart transplantations were performed.We summarized surgical technique after sixteen rats of preliminary experiment.The successful rate was improved to 96.88% in 32 rats of formal transplantation.Time of cold ischemia,hot ischemia and rebeat were shorten significantly and operational time reduced to 60 min.Conclusion:To strengthen the protection of donor heart and perioperative treatment is the key point in the heart heterotopic transplantation in inbred strain rat.

Single incision laparoscopic surgery (SILS) has many advantages than standard multiport laparoscopic cholecystectomy (MLC),such as small trauma,less postoperative pain,shorter hospital stay,good cosmetic effect,and so on.Especially the satisfying cosmetic result of no abdominal scar is more important.But the SILS is also faced with many difficulties.The operation time of SILS were significantly longer in duration than MLC.The safety and the technical feasibility were lower for the SILS whose operation complications are more than the MLC.The main reason is that the operation field don't exposureis sufficient and the formation of surgical operation triangle is not easy.In order to overcome these difficulties,scholars have used the auxiliary methods of exposing the operative field in various operation,including the penetration of abdomen wall retraction and intraperitonealretractionand abdomen wall retraction.This paper with review the advantages and disadvantages of the above methods aiming to affer more values for clinical doctors in opperating SILS who get more knonledge abont it.

Objective To study the efficacy of complement depletion with CVF in preventing a-cute rejection after heart allotransplantation in inbred strain rats. Methods Inbred male Lewis rats were used as recipients and Brown-Norway rats as donors, and the heterotopic heart transplantation model was established. The allografts were divided into 2 groups (n=8 in each group). After infusing low-dose CVF 20?g/kg to the CVF-treated group, cardiac allograft survival time was observed on 4 rats of each group. The remaining 4 rats in each group were killed respectively at day 1,3,5 and 6, the pathological grade for acute rejection, the complement activity in serum, the deposition of C3 on tissue, and the extent of infiltration by CD3+ T cells were compared. Results The mean survival time of heart allograft was (11. 69?0. 72) days and (6. 65?0. 35) days in CVF-treated group and control group respectively (P

Adult ; Carcinoma, Small Cell ; pathology ; Female ; Humans ; Male ; Melanosis ; pathology ; Uterine Cervical Neoplasms ; pathology

OBJECTIVETo explore the molecular mechanisms of main active ingredients of Siwu decoction analogous formulae for treating primary dysmenorrhea of gynecology blood stasis syndrome by network pharmacology study, and to investigate the correlations between multi-compounds, multi targets and multi pathways.

METHODMajor active compounds from Siwu decoction analogous formulae, including ligustilide, butylidene phthalide, senkyunolide, ferulic acid, gallic acid, peoniflorin, jioglutin A, catalpol, transanethole, zingiberone, commiphoric acid, eugenol, isorhamnetin-3-O-neohesperidoside, wulingzhic acid, alpha-cyperone, cyperene, costunolide, costic acid, tetrahydropalmatine, protopine, amygdalin, 24-methylene cycloartanol, oleic acid, linoleic acid, 3-p-coumaroylquinic acid, hydroxysafflor yellow A, coptisine, berberine, jatrorrhizine, baicalein, baicalin, wogonin were collected to build component-protein networks based on PharmMapper database. The targets information access was used to construct and visualize components-targets-pathways network model using the kyoto encyclopedia of genes and genomes (KEGG) pathway database and Cytoscape software.

RESULT AND CONCLUSIONSerine threonine protein kinases play an important role in the process of cells. They were potential targets in the effect of Siwu decoction analogous formulae. The effect of main active ingredients involved 51 the pathway. Besides the same ones, Shaofu Zhuyu decoction had more effect on lipid metabolism, Xiangfu Siwu decoction on amino acid metabolism pathways, Taohong Siwu decoction on carbohydrate metabolism, while, Qinlian Siwu decoction on ErbB, VEGF signal transduction pathway. Siwu decoction and its derived formulae not only had common targets and pathways, but also had their own emphasis. This reflected the formulae effect mode of multi-ingredients, multi-targets and multi-pathways. It may provide clues to deeper study of molecular mechanism of Siwu decoction analogous formulae action.

Drugs, Chinese Herbal ; chemistry ; pharmacology ; Dysmenorrhea ; drug therapy ; genetics ; metabolism ; Female ; Gynecology ; Humans ; Medicine, Chinese Traditional ; methods ; Signal Transduction ; drug effects ; genetics

Adolescent ; Aged ; Aspergillosis ; microbiology ; pathology ; Aspergillus ; isolation & purification ; Brain Diseases ; drug therapy ; microbiology ; pathology ; surgery ; Diabetes Complications ; microbiology ; Diagnosis, Differential ; Female ; Humans ; Male ; Mucorales ; isolation & purification ; Mucormycosis ; drug therapy ; pathology ; surgery ; Nose Diseases ; drug therapy ; microbiology ; pathology ; surgery

Objective To investigate the response of astrocytes and neurons in the medullary visceral zone (MVZ) to neuropathic pain induced by the spared nerve injury (SNI, the tibial nerve and the common peroneal nerve were sectioned, while the sural nerve was intacted). Methods The SNI operation and sham operation (only incised the skin of thigh, but the tibial nerve and the common peroneal nerve did not cut) were performed in adult male Sprague-Dawley rats. The paw withdrawal mechanical threshold (PWMT) was measured at 10,20,30 days after operation. By using single or double immunofluorescent staining method,we investigated and measured that the mean fluorescent intensity (MFI) of anti-glial fibrillary acidic protein (GFAP, a marker of astrocytes) signal in the astrocytes, the mean member of Fos/ GFAP double labeled astrocytes and Fos / tyrosine hydorxylase (TH) double labeled neurons in MVZ after operation. Results As compared with control or sham groups, SNI induced that the PWMT became significant sensitivity and peaked at 20 days after SNI. The activated astrocytes revealed an activated morphology, the MFI of anti-GFAP signal markedly strengthened, the mean number of Fos/GFAP double labeled astrocytes and Fos/TH double labeled neurons in the nucleus of the tract solitarius (NTS) and the ventral lateral medulla (VLM) increased significantly and peaked at 20 days after SNI. Conclusion The neurons and astrocytes in MVZ were sensitively activated by SNI.

Objective To study whether the early continuous passive motion (CPM) can prevent the degeneration of articulation after the articular cartilage was experimentally defected, and the effect of the different durations of continuous passive motion therapy. Methods Fifteen New Zealand rabbits were used to establish the model of full-thickness defects of bilateral knee articular cartilage by operation. Then all the animals were divided into groups A, B and C: Group A served as the control group, and groups B and C served as the observation groups and were administered with 2 hours of continuous passive motion a day (group B) and 8 hours of continuous passive motion a day, respectively. All rabbits were sacrificed at the 12th week after operation, and their articular cartilage were observed. The cartilage and synovium were obtained to have the hematoxylin and eosin stain and immunohistochemistry stain. After that, the pathological assessment and calculation of the ratio of MMP-3 positive cells were conducted and compared among groups. Results Group C was lower than groups A and B in terms of the scores with cartilage pathological assessment and the ratio of MMP-3 positive cells(P

OBJECTIVE:To establish a method for the content determination of kaempferol in Kaempferia galanga. METH-ODS:HPLC was performed on the column of Diamonsil ODS2 C18 with mobile phase of methanol-0.4% Phosphoric acid solution at a flow rate of 1 ml/min,detection wavelength was 367 nm,column temperature was 30℃,and injection volume was 10 μl. RE-SULTS:The linear range of kaempferol was 0.001 58-0.158 mg/ml;RSDs of precision,stability and reproducibility tests were low-er than 3%;recovery was 95.52%-99.32%(RSD=1.47%,n=6). CONCLUSIONS:The method is simple,accurate and reproduc-ible,and can be used for the content determination of kaempferol in K. galanga.

Radiation therapy is one of the important comprehensive treatment strategies for thoracic neoplasms,but it also can cause radiation-induced heart disease (RIHD) which can affect the quality of life and even endanger the lives of patients while killing tumor.With the development of comprehensive treatment for cancer,the patients achieve better survival outcomes.And the study reported about RIHD increased as well,which made RIHD became an important complication of thoracic neoplasms' treatment.Though modem radiotherapy techniques and new therapeutic principles have significantly made the incidence of RIHD decreased,the harm of RIHD should not be ignored.In this article,we review the research progress of RIHD.