The importance of designing experiments in the reform of experimental teaching was expounded.The achievements of reformation and exploration in the selection of experimental items,the improvement of teaching methods,the advancement of teachers' levels and the examination of students' grades in researching and designing experiments in pharmaceutical analysis were summarized comprehensively.

Objective To study the analgesic effect of sufentanil combined with Dezocine in endoscopic resection of fallopian.MethodsA total of 188 patients with tubal pregnancy from April 2013 to December 2014 in Haining central hospital were enrolled in the study, they were randomly divided into two groups (n=94).On the basis of conventional intravenous analgesia, the patients in control group was were received sufentan, the observation group were given sufentan combined with dezocine.The score of pain at different time points after operation, the Ramsay sedation score, and the level of IL-6 and IL-10 were compared.The adverse reactions were compared between the two groups.ResultsThe score of VAS in the observation group was significantly lower than that in the control at 4h, 8h, 12h and 24h after operation (P<0.05).The score of VAS in the two groups were similar at 48h after operation;the Ramsay score in the observation group was significantly higher than that in the control group(P<0.05) at five time points (4h, 8h, 12h, 24h and 48h after operation);when compared Compared with the indexes before operation, the level of IL-6 was significantly increased in the two groups at 24h and 48h, but the observation group increased less, the IL-6 in observation group was lower than that in the control group at the two points(P<0.05);while the IL-10 was higher than that beforeoperation, and the observation group was more higher(P<0.05).The adverse reactions in the two groups were mainly dizziness, nausea, pruritus and lethargy, and the incidence was only 12.8% in the observation group, which lower than that in the control of 29.8%(P<0.05).ConclusionIt which dazocine combined with sufentanil had a good analgesic effect in the process of analgesia in gynecological laparoscopic resection of fallopian tube after the operation.The VAS score and Ramsay sedation score was improved significantly.The levels of inflammatory factors were lower, and the incidence of adverse reaction was significantly reduced.It is a reliable method for clinical intravenous analgesia.

Objective To observe the effect of the first iodine-131 ablation of thyroid remnants in pa-tients with differentiated thyroid cancer (DTC) and discuss factors that may be influential in the treatment. Meth-ods All DTC patients confirmed by pathology received iodine-131 therapy after necessary preparation. Accord-ing different operation procedures, time interval between operation and iodine-131 treatment and dose of iodine - 131, the patients were divided into different groups. Therapy effects were observed in different groups. Results Ablation successful rate was 88.5% in total thyroidectomy group, compared with 44.4% in subtotal thyroidecto-my group. 92.7% ablation rate was seen in the group of I-2 months time interval between operation and iodine - 131 treatment, while in the group of over 1 year time interval, only 50% ablationrate was achieved. Ablation successful rate was 47.6% in the group of low dose of iodine-131 (2.96GBq), compared with 82.8% in the high dose group (4.44GBq). Conclusions Total thyroidectomy and cervical lymph nodes dissection should be recommended for the patients with DTC. The better interval time between operation and iodine-131 ablation thy-roid remnants is 1～2 months. The high dose of iodine-131 shows better result than low dose of iodine-131 treatment.

Aged ; Antigens, CD ; metabolism ; Antigens, Differentiation, Myelomonocytic ; metabolism ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Histiocytosis, Sinus ; pathology ; surgery ; Humans ; Immunoglobulin G ; blood ; Paranasal Sinus Diseases ; pathology ; surgery ; Paranasal Sinuses ; pathology ; S100 Proteins ; metabolism ; Sclerosis ; pathology

Adolescent ; Adult ; Amnion ; transplantation ; Blood Transfusion, Autologous ; Eye Burns ; therapy ; Humans ; Male ; Middle Aged ; Occupational Injuries ; therapy ; Young Adult

Objective To investigate the role of intraeellular Ca2+ and MERTK in the phagocytosis of human retinal pigment epithelial (RPE) cells, and reveal the relationship between MERTK and intraeellular Ca2+. Methods The euhured RPE cells were incubated with rod outer segments (ROS) at 37℃, the phagoeytosis was terminated at different incubation time points. The concentration of intraeellular Ca2+ was assayed by Fluro-3/AM loading methods combined with fluorescence microscope and CCD system, and the mRNA level of MERTK gene was measured by reverse transcription polymerase chain reaction (RT-PCR). Treating the RPE cells with stimulator (A23187)or inhibitor(verapamile)of intraeellular Ca2+ to observe the changes of MERTK gene expression. Results ROS adhered to hRPE cells at the 15th minute, and the ingestion saturated at the 24th hour. The concentration of intracellular Ca2+ increased at the 15th minute, and kept the high level in 24 hours. The level of MERTK mRNA increased at the 5th minute, and kept the high level duration the whole incubation. When RPE cells were treated by A23187, the expression of MERTK increased in a dose-dependent manner. After RPE cells was pretreated by A23187, the expression level of MERTK was higher in the proceeding incubation groups than which in the control group except at the 3rd hour. When RPE cells were treated by verapamil, the expression level of MERTK decreased in a dose-dependent manner. After RPE cells were pretreated by verapamil , the expression level of MERTK was lower in all the proceeding incubation groups than which in the control group (P<0.05). Conclusion MERTK gene and Ca2+ play an important role in sustaining RPE cells phagoeytizing ROS. As an up-stream regulator, the receptor tyrosine kinase MERTK keeps RPE cells phagocytizing ROS by starting the intracellular Ca2+.

OBJECTIVETo prepare matrine double-sensitive colon-specific pellets and study the factors affecting its quality and evaluateing the colon-specific effects of preparation.

METHODMatrine enzyme-sensitive pellets core were prepared by carboxymethyl konjac glucomannan as the main carrier material, and coated the core by acrylic resin II and III to prepare matrine double-sensitive colon-specific pellets. The prescription and technology of the matrine colon-specific pellets were studied by the single factor investigation, through the in vitro release test and coating rate determination.

RESULTThe optimized process conditions: FeCl3 concentration is 4.0 g x L(-1), chitosan concentration is 3.0 g x L(-1), carboxymethyl konjac glucomannan concentration is 20 g x L(-1), mixed gel solution pH value is 3. The release of matrine is less than 30% in the simulation of the upper gastrointestinal medium. The release of matrine is close to 100% in simulated full gastrointestinal medium, the coating weight is 7%.

CONCLUSIONThe prepared pellets have good colon positioning effect in vitro.

Acrylic Resins ; chemistry ; Administration, Oral ; Alkaloids ; administration & dosage ; chemistry ; pharmacokinetics ; Chitosan ; chemistry ; Chlorides ; chemistry ; Colon ; metabolism ; Delayed-Action Preparations ; administration & dosage ; chemistry ; pharmacokinetics ; Drug Compounding ; methods ; Drug Delivery Systems ; methods ; Ferric Compounds ; chemistry ; Humans ; Hydrogen-Ion Concentration ; Mannans ; chemistry ; Quinolizines ; administration & dosage ; chemistry ; pharmacokinetics ; Reproducibility of Results ; Tablets, Enteric-Coated ; Time Factors

Objective To study the effect of propofol on vascular reactivity and plasma malondialdehyde (MDA) and nitric oxide (NO) in septic shock.Methods Forty male SD rats weighing 200-250 g were randomly divided into 4 groups (n = 10 in each group) : group Ⅰ control; group Ⅱ septic shock; group Ⅲ septic shock + propofol and group Ⅳ septic shock + melatonin. The animals were anesthetized with intraperitoneal pentobarbital 40 mg?kg-1. The femoral artery and vein were connulated for MAP monitoring and drug administration. The animals were breathing spontaneously. Septic shock was induced by intravenous LPS 15 mg?kg-1 . In group Ⅲ a bolus of propofol 10 mg?kg-1 was given i.v. at 1 h after intravenous LPS followed by intravenous propofol infusion at 10 mg?kg-1?h-1. In group Ⅳ melatonin 10 mg was given intraperitoneally at 1h after LPS i.v. . Six hours after LPS administration 4 doses of phenylephrine (PE) 0.5, 1, 2, 2.5 ?g?kg-1 were given i.v. in succession. The next dose was given when MAP returned to the baseline level after previous PE. The percent change in MAP after each dose was recorded. Blood samples were taken at 6 h after LPS administration for determination of plasma MDA and NO concentrations. After the in vivo experiment the animals were sacrificed and thoracic aortas were removed and cut into segments of 3 mm in length which were bathed in Krebs buffer aerated with 95% O2 and 5% CO2 at 37℃. The aortic rings were stretched to a resting tension of 2.0 g. The segments were then exposed to increasing concentrations of PE (from 1 nmol?L-1 to 30 ?mol?L-1). The dose-response curves were obtained. Emax and EC50 were calculated. Results The percent increase in MAP induced by PE was significantly reduced by septic shock (groupⅡ,Ⅲ,Ⅳ) as compared with control group (Ⅰ), but was significantly larger in propofol and melatonin groups (Ⅲ,Ⅳ) than in group Ⅱ. In the in vitro experiment the maximum response to PE and EC50 were significantly reduced in rats with septic shock as compared with rats in control group (P

[Objective]To discuss the injury severity and treatment of 236 patients with upper limb injuries in Wenchuan Earthquake.[Method]The basic informations of patients with upper limb injures in Wenchuan Earthquake,and the injury mechanisms,clinical symptoms and treatment were collected and analyzed.[Result]In 236 cases of patients with upper limb injuries,there were 208 patients,378 cases with fracture,and 64 cases with open fracture.There were 35 cases of clavicle fracture,31 cases of scapula fracture,113 cases of humerus fracture,46 cases of ulna fracture,87 cases of radial fracture and 66 cases of carpometacarpai phalangeal fracture.Fifty cases received debridement and suture,60 cases received fracture reduction and internal fixation and 7 cases received external fixation.Totally,19 limbs were carried on amputation,and only one patient died of hyperkalemia and combined injury.[Conclusion]Analysis of patients with injury mechanisms,clinical symptoms and treatment can provide advices for rescuing and treating of patients with upper limb injuries in earthquake or other disaster cases.

Objective:To study the expression and clinical significance of hypoxia-inducible factor-1? (HIF-1?) in squamous cell carcinoma of buccal mucosa.Methods:45 cases of paraffin embedded samples of squamous cell carcinoma of buccal mucosa were examined with HIF-1? antibody by immunohistochemistrical method. The relationship between HIF-1? expression and clinico-pathological factors such as sex, age, duration of tumour, T classification, differentiation, lymph node metastasis in the neck, recurrence, clinic classification, tumour thickness, the number of mitosis and the degree of lymphocyte invasion was analyzed.Results:The positive expression of HIF-1? was found in 31 out of the 45 cases(69%). HIF-1? positive reaction was predominately located in the center of tumour nest. While analyzing clinico-pathological factors, only T classification (between T1+T2 and T3+T4, P=0.008), lymph node metastasis of the neck (P=0.037) and clinic classification (between stage I+II and III+IV, P=0.011) showed a significant correlation with HIF-1? expression. HIF-1? possitive expression is higher in T3+T4(d=4.87 mm) than that in T1+T2(3.81 mm) (P=0.046). Logistic analysis showed that the longer the patients lived after operation, the less the HIF-1? positive expression.Conclusions:HIF-1? positive expression in squamous cell carcinoma of the buccal mucosa correlates to T classification, lymph node metastasis of neck, clinical classification and survival