Objective To investigate the efficacy of metholrexate combined with rmifepristone and laparoscopic surgery for treating ectopic pregnancy.Methods 85 patient with ectopic pregnancy were selected.41 cases treated with methotrexate and mifeprisione were considered as the control group,while 44 cases with laparoscopic surgery were considered as the observalion group on the voluntary basis.The clinical efficacy,serum β-HCG value and recovery time after 7d were compared between the two groups.Results After the treatment,the cure rate in the observation group was 100％ which was significantly higher than 90％ of the control group ( P ＜ 0.05 ),the serum β-HCG value of the observation group was lower and the restoration normal time was shorter than the control group ( P ＜0.05).Conclusions Laparoscopic surgery and conservative treatment of methotrexate combined with mifepristone are effective in the treatment of ectopic pregnancy.However,the clinical efficacy of the laparoscopic surgery is better and it has rapid recovery time,and it can completely cure the patients.

Objective:To observe the effect of the signaling pathway of protein kinase C (PKC)-nuclear factor-erythroid 2-related factor 2 (Nrf2) on the expression of heme oxygenase -1 (HO-1) induced by cigarette smoke extract in rat airway epithelial cells.Methods:The airway epithelial cells of 25 male SD rats were randomly divided into a control group, a CSE3h group, a RO318220 group (PKC inhibitor), a Nrf2 siRNA group and a Nrf2 siRNA+RO318220 group, 5 rats in each group. hTe control group was incubated with DMEM/F12 alone. hTe CSE3h group was treated with 10% CSE for 3 h. hTe RO318220 group was pretreated with 3 μmol/L RO318220 for 0.5 h and subsequently treated with 10% CSE for 3 h. hTe Nrf2 siRNA group was pretreated with Nrf2 siRNA, and then treated with 10% CSE for 3 h. hTe Nrf2 siRNA+RO318220 group was pretreated with Nrf2 siRNA and 3 μmol/L RO318220 for 0.5 h, and then treated with 10% CSE for 3 h. hTe protein levels of Nrf2 in the nucleus and cytoplasm, and HO-1 and PKC in the whole cells were semi-quantified by Western blot. The protein expression of HO-1 was measured by immunocytochemistry. HO-1 mRNA expression was detected by RT-PCR. Immunolfuorescence staining was used to observe the nuclear translocatin of Nrf2. Results: CSE markedly induced Nrf2 nuclear translocation in the rat airway epithelial cells, and RO318220 pretreatment blocked the CSE induced Nrf2 nuclear translocation. Immunocytochemistry showed that HO-1 protein expression was strongly positive in the CSE3h group, weakly positive in the other 4 groups. hTe expression of PKC protein, HO-1 mRNA and protein signiifcantly increased in the CSE3h group, and HO-1 activity markedly improved in the CSE group (P<0.05). hTe level of PKC protein expression was not signiifcantly different in the Nrf2 siRNA group compared with that in the CSE3h group (P>0.05). Conclusion: CSE induces the nuclear translocation of Nrf2 by PKC signaling pathway, thus upregulating the HO-1 expression in the rat airway epithelial cells.

[ABSTRACT]AIM:ToobservetheeffectofthesignalingpathwayofproteinkinaseC(PKC)-nuclearfactor-ery-throid 2-related factor 2 (Nrf2) on the expression of heme oxygenase-1 (HO-1) induced by cigarette smoke extract (CSE) in the rat airway epithelial cells .METHODS:After exposed to CSE , the airway epithelial cells of male SD rats were di-vided into control group , CSE3 group, RO318220 group, Nrf2 siRNA group and RO318220+Nrf2 siRNA group.The pro-tein levels of HO-1, Nrf2 and PKC were semi-quantified by Western blotting .The protein expression of HO-1 was assessed by immunocytochemistry .The mRNA expression of HO-1 was detected by reverse transcription-polymerase chain reaction ( RT-PCR) .Nrf2 protein location was observed by immunofluorescence staining .HO-1 activity was also determined .RE-SULTS:After exposure to CSE for 3 h, the Nrf2 protein was mainly located in the nucleus , and the expression level of Nrf2 protein in the nucleus was stronger than that in the control cells .CSE also significantly enhanced the levels of p-PKC protein, and HO-1 mRNA, protein and activity .Pretreatment with RO318220 significantly decreased the levels of PKC pro-tein, Nrf2 protein, and HO-1 mRNA, protein and activity.Meanwhile, Nrf2 protein significantly decreased , and the activ-ity, mRNA and protein levels of HO-1 were also significantly attenuated by pretreatment with siRNA to knock down Nrf2. Pretreatment with RO318220 and Nrf2 siRNA significantly decreased the PKC protein , Nrf2 protein, and HO-1 mRNA, protein and activity .CONCLUSION:CSE up-regulates the HO-1 expression through PKC-induced nuclear translocation of Nrf2 in the rat airway epithelial cells .

The course of audiology is both important and difficult to the undergraduate education of otolaryngology.Through a series of reforms in teaching contents and methods of audiology,the students'effect of study were greatlyimproved,and thus these reforms were generally appreciated by both teachers and students. In conclusion, the act of theteaching reform not only advanced the students' learning enthusiasm and gumption, but also helps the development andexercise in their clinical thinking ability.

Humans ; Liver Neoplasms ; surgery ; Perioperative Care

An artificial liver support system (ALSS) is a body circulatory system aims to blood purification, which consists of plasma exchange, hemodialysis, hemoperfusion, in combination with hemodiafiltration. The clinical application of non-biological artificial liver, especially plasma exchange technology, can solve the problem such as hyperbilirubinemia, endotoxemia, toxic substance accumulation and disorder of amino acid metabolism, which would save patients’ lives. ALSS can significantly improve the general physical condition, reduce operative risk, as well as relieve physiologic derangement after liver transplantation. Therefore, artificial liver serves as a bridge for liver transplantation. This paper analyzes the characteristics of ALSS, explores the methods of ALSS application in perioperative period of liver transplantation, and discusses its developing orientation.

Objective To study the influence of Mutouhui extracts on hemorheology of U14 cervical cancer mice.Methods The U14 cervical cancer mice were divided into Mutouhui extracts high,middle and low dose group,Cisplatin Injection group,model group and blank comparing group.The influence on hemorheology change was observed after being given the medicine.Results The high,middle dose group of Mutouhui extracts and Cisplatin injection group could reduce the blood viscosity of U14 cervical cancer mouse in different shear,and hemotcrit of the low did not obviously change,groups of Mutouhui extracts plasma viscosity changed slightly.Cisplatin Injection group could reduce the blood viscosity,but did not obviously affect to the plasma viscosity.Conclusion Mutouhui extracts can reduce the U14 cervical cancer mouse blood viscosity,and improve the indexes of hemorheology.

Gradient System is one of the most important parts of Magnetic Resonance Image System.It is a key factor in the image quality.Therefore,during the whole installation process,advanced gradient system is strictly needed.Firstly,this article gives a clear explanation about the theory and function of the gradient system.Then,it discusses how to make rational use of gradient system to acquire the best image quality.

Objective To design a handy labor monitor based on ARM hardware structure so that the condition of pregnant women can be reflected more accurately and completely.Methods The host-slave mode was adopted,which was composed of host machine based on ARM and embedded WinCE operating system.The slave machine used the 51 single chip to acquire data.Results This system was convenient and stable,and the interface is friendly.Conclusion The study of this system can be used for reference for the development of related data acquisition machine.[Chinese Medical Equipment Journal,2008,29(2):31-33]