Air Pollutants ; Cardiovascular Diseases ; epidemiology ; Humans ; Particulate Matter

Objective: To establish an HPLC method for determination of baicalin and wogonoside contents in Xiaochaihu decoction. Methods: Decoction pieces were mixed and decocted with water. The chromatographic column was Agilent Zorbax XDB-C18 (150 mmX4.6 mm, 5 μm); the mobile phase was composed of 0.2% phosphate acid-water(A) and acetonitrile (B) with gradient elution at a flow rate of 1 ml/min. The detection wavelength was set at 275 nm, the temperature of column was 25°C, and the injection volume was 15 μL. Results: Baicalin and wogonoside were separated at baseline within 30 min with good linearity; the standard curves for baicalin and wogonoside were Y=44.16X-36.22 (r=0.999,9) and Y=52.08X-28.69(r= 0.999,9), respectively. The intra-day and inter-day precisions were both less than 1%, and the limits of qualification were 0.615,6 μg/ml for baicalin and 0.220,8 μg/ml for wogonoside. The recovery rates (n = 6) were 95.73% (RSD = 0.8%) for baicalin and 97.02% (RSD= 1.56%) for wogonoside. Conclusion: The method is simple, accurate, stable, and reliable in determining the contents of baicalin and wogonoside in Xiaochaihu decoction, and it can be used for the quality control of this preparation.

Objective: To determine the contents of calycosin-7-O-β-D-glucoside and formononetin in Astragalus membranaceus (Fisch.) Bge. by high performance liquid chromatography (HPLC). Methods: The HPLC condition was as follows: column: Hypersil ODS 2 column (4.6 mm×250 mm, 5 μm); mobile phase: A was ACN-MeOH (9 : 1,V /V), B was H2O, with gradient elution; flow speed: 1.0 ml/min; detection: 260 nm; temperature of column: room temperature; injection volume: 20 μl. Astragalus membranaceus (Fisch.) Bge. was extracted with methanol solution twice, each time 20 min. Results: The theoretical plate numbers of calycosin-7-O-β-D-glucoside and formononetin were 50 134 and 25 258, respectively. The calibration curves were linear within the range of (2.022-101.1) μg/ml for calycosin-7-O-β-D-glucoside and (38.04-1522) μg/ml for formononetin, with their regression function being Y=58 924X - 12 352, r=0.999 9 and Y=9 237X - 124 447, r=0.999 9, respectively. The intra-day and inter-day precisions (RSD) at low, middle and high injection volume were all less than 2.0%. The limits of detection were 0.202 2 mg/ml for calycosin-7-O-β-D-glucoside and 1.522 mg/ml for formononetin. The recovery rates were 98.34% (RSD=1.33%, n=3) for calycosin-7-O-β-D-glucoside and 98.84% (RSD=0.12%, n=3) for formononetin. The contents of calycosin-7-O-β-D-glucoside and formononetin in 10 different batch of Astragalus membranaceus (Fisch.) Bge. were determined. Conclusion: HPLC is a simple and reliable method for determining the contents of calycosin-7-O-β-D-glucoside and formononetin in Astragalus membranaceus (Fisch.) Bge.

Air Pollution ; Atherosclerosis ; etiology ; Humans ; Particulate Matter

Concern on antibiotics contamination has been raised because of its serious environmental impact in recent years.The application of antibiotics,measurement method for antibiotics,mechanism of antibiotics degradation,and impact of antibiotics contamination on environment and human health were reviewed and commented.The advisable solution and future researches were prospected in this review,too.

Objective To investigate the expression of CD2 in peripheral blood T lymphocytes in acute rejection after small bowel transplantation (SBT) in rats.Methods Three groups were set up: Group A (n=18), receiving sham operation and feeding of common feedstuff; Group B (n=18) receiving SD rat to SD rat iso-series SBT and the treatment of routine liquid infusion and antibiotics after operation; Group C (n=18) receiving SD rat to Wistar rat taniso-series SBT and the same post- operation treatments as in group B. Samples of grafts and peripheral blood in the three groups were collected on the day 3, 5 and 7 respectively after SBT for detection of flow cytometry. The intestinal grafts were obtained for pathological examination.Results The survival time of rats in group C, group B and group A was 7.0? 2.1, 33.3? 2.3 and more than 90 days, respectively. There was significant difference between group C and groups A, B (P

Objective To evaluate and compare 3 kinds of cecocolon urinary reservoir. Methods Penn pouch,indiana pouch or detenial cecocolon reservoir has been performed for 37 patients.The out-come was evaluated by urodynamic and clinically. Results Good continence has been achieved in all patients with an appendiceal continence mechanism.The capacity and intrareservoir pressure of detenial cecocolon was significantly different from penn or indiana pouch 3 months postoperatively (P0.05).The intrareservoir pressure on contraction was lower with penn pouch. Conclusions The penn or modified indiana pouch is better and more ideal as an urinary reservoir whereas the detenial cecocolon pouch is technically simpler.

This paper presents a high voltage generator for the defibrillator.Its principle and design method are introduced.The high voltage generator is the core part of the defibrillator which is necessary toany hospital.There is nodomestic-patented defibrillator by now and published paper specialized in the principle and design method of the high voltage generator for the defibrillator.

Objective To summarize the practical experience of 2 cases of anemia following living related intestinal transplantation.Methods The clinical data of 2 cases of anemia following living related intestinal transplantation were studied retrospectively. After the surgical procedure regiments of blood transfusion, hemostasia, to cut down the dosage of some drug, to fill up raw material of hematopoientic system and requisite nutrition support were used to treat the patients. Results Four weeks later, the diseased conditions of the two patients became better and better. The index of hemoglobin were increased remarkably, to normal level 100 to 120?g/L.Conclusion Anemia is one of the common syndrome in living related intestinal transplantation. The correction of anemia is very important in treating such patients.

Objective To explore the anatomy and the adjacent of the medial malleolus,the operative approach of soluting the tibial nerve in endoscopy and the significance of making use of it in operation. Methods The five cadaver feet were dissected,the tibial nerve and the fiber diaphragm in the middle of me- dial and lateral plantar tunnels in the ten cadaver feet were soluted in endoscopic.Results The operative approach was designed on the basis of the anatomy data (the three-portal approach:The perpendicular bisetor which was the medial malleolus pointed to the medial margin of tendon calcaneus insertion was 4.5cm;The midpoint of the medial malleolus pointed to the calcaneus tubercle;The midpoint of the calcaneus tubercle to the medial margin of the first metatarsophalangeal joint).It was succeed that soluted the tibial nerve and the fiber diaphragm in the middle of medial and lateral plantar tunnels.Conclusion The operative approach on the basis of the anatomy data is design,and testfy the feasibility of exploring curved tunnel in endoscopic.