Vascular Endothelial Growth Factor and its receptors may be the pivotal stimulants in the process of growth and metastasis of a neoplasm. They boost the genesis of the blood vessels of brain glioma. Their expression is correlated with the grading of glioma. They are ideal targets in the treatment of inhibiting the growth of the blood vessels of brain glioma.

Objective To investigate the expression of RCAS1,which is a receptor-binding cancer antigen expressed on SiSo cells in many tumor cell lines. Methods Using real-time polymerase chain reaction(PCR),Northern blot,immunohistochemical examination and enzyme-linked immunosorbent assay,we detected the expre-(ssion) of RCAS1 in 10 different tumor cell lines and two normal cell lines. Results The expression of RCAS1 in the tumor cell lines and the fetal renal cell line(293 cell) was detected by real-time PCR and Northern blot,but not in the normal liver cell line(LO2 cell).The results of immunohistochemical examination on the tumor cell lines and the 293 cell showed that RCAS1 existed in cytoplasma and on cell surface.Except HuH-7 cell and LO2 cell,the soluble RCAS1 protein in supernatants of the other cells was significantly more than that in DMEM(P

AIM: Using surface active agent that has wetting ability and solubilising to increase the extraction rate of curcumin. METHODS: Different surface active agents, such as span, span 60, tween 20, tween 85. SDS and CSBS, were added and then the curcumin was determined. RESULTS: When we added 0.5% sodium dodecyl sulfate (SDS), it could increase the extractive rate of curcumin by 16%. CONCLUSION: Different surface active agents have variable solubiitzation of different drugs. SDS can affect the extractive rate of curcumin.

To explore the opportunities and the challenges on continuing medical education of health technique cadres of armed police force,the article discussed the countermeasures of con-tinuing medical education of armed police force,in order to develop the work of continuing medical education of armed police force,which offered the methods and references for bringing up the ex-cellent health technique cadre of armed police force.

Humans ; Laryngeal Neoplasms ; surgery ; Larynx ; surgery ; Laser Therapy ; Microsurgery

Objective To investigate the clinical efficacy of off-pump coronary artery bypass grafting (OPCABG) comparing with the conventional coronary artery bypass grafting (CCABG) for treating the patients with coronary heart disease (CHD) and its impact on brain natriuretic peptide (BNP).Methods One hundred and twenty-two patients undergoing elective coronary artery bypass grafting were divided into CCABG group and OPCABG group according to the surgical method.The operative condition and postoperative clinical data,postoperative complications and death of the two groups were observed.The level of BNP was detected before surgery,immediately after surgery,postoperative 6,24,72 h and 1 week.Results The operative time,postoperative mechanical ventilation time,ICU monitoring time,24 h after drainage and blood transfusion,hospital stay in OPCABG group was(210.08 ± 60.02) min,(9.01 ± 2.57) h,(32.08 ±9.17) h,(343.43 ± 98.12) ml,(341.75 ±97.64) ml,(9.70 ±2.77) d,significantly lower than those in CCABG group [(309.38 ± 88.39) min,( 15.25 ±4.36) h,(45.14 ± 12.90) h,(530.24 ± 151.50) ml,(752.90 ± 215.11 ) ml,( 15.44 ± 4.41 ) d] ( P ＜ 0.05 ).The incidence of postoperative complication of OPCABG group and CCABG group was 15.9％(10/63) and 47.5％(28/59),there was significant difference ( χ2 =14.172,P ＜ 0.01).The mortality rate of OPCABG group and CCABG group was 1.6％(1/63) and 8.5％(5/59),there was no significant difference ( x2 =3.091,P ＞ 0.05 ).The level of BNP in CCABG group before surgery,immediately after surgery,postoperative 6 h was (104.54 ±29.87),(114.74 ±32.36),( 129.10 ± 36.15 ) ng/L,and in OPCABG group was ( 103.46 ± 29.56 ),( 109.49 ± 31.28 ),( 126.42 ± 36.12 )ng/L respectively,there was no significant difference (P ＞ 0.05).The level of BNP in CCABG group postoperative 24,72 h and 1 week [(335.57 ± 95.83 ),(429.98 ± 122.85 ),(350.92 ± 100.26) ng/L] were significantly higher than those in OPCABG group [(241.22 ± 68.92 ),( 317.49 ± 90.71 ),(256.86 ± 73.39)ng/L] (P ＜ 0.05).The levels of BNP in both groups postoperative 24,72 h and 1 week were significantly higher than those before surgery (P＜ 0.05).Conclusion The OPCABG surgery is safe and effective,and has certain advantages for maintenance of cardiac function.

Objective To investigate the diagnostic value and feasibility of PTC diagnosis on pancreaticobiliary maljunction (IBM), and to summarize the PTC radiological characteristics of PBM. Methods Clinical findings and cholangiopancreatographic results were analyzed retrospectively for a group of consecutive 363 patients with obstructive jaundice receiving the PTCD therapy. Meanwhile the standard selected for cases and diagnostic conditions were established. The length and diameter of pancreaticobiliary common duct, the diameter of pancreatic duct and common bile duct and the confluence angle were measured respectively. The t test and rank sum test were used to analyze the result statistically. Results Thirty-eight cases were radiologicaUy diagnosed as PBM owing to the reference standard and the detection rate was 10.5% (38/363). The length of common duct was (12.6±7.9)mm. The significant difference existed between it and normal value (6ram) (t=5.15 , P <0.05). The site of duodenal papilla had influence on the length of common duct. The diameter of common bile duct, pancreatic duct and common duct near the confluence are (3.7±1.9 ) mm, (2.4±1.3) mm, (3.3±1.4 ) mm, respectively. There was no statistical difference between them and the normal value (t=1.79,2.85,5.72, P>0.05). Fifteen patients' duodenal papilla located the middle of descending duodenum. The length of common duct was (10.6±9.1)mm , the confluence angle was 51.1°±28.0°, the number of the duodenal papilla locating in the inferior 1/3 of descending duodenum, juncture , horizontal part of duodenum was 10, 8,5, respectively. The length of common duet were (9.9±3.7), ( 18.6±8.9), ( 13.9±3.5 ) mm, respectively. The confluence angle were 54.0°±18.6°、48.7°±12.6°、74.4°±18.5°, respectively . The site of duodenal papilla had significant influence on the length of common duct(X2=14.51, P <0.05). Conclusion PTC is a safe, feasible, method to diagnose PBM, and it demonstrates the characteristic findings of PBM.

Objective To investigate the neonatal death causes and the relationship between the causes and the neonatal age,fetus age and the year in order to reduce neonatal mortality.Methods Data of neonatal autopsy and pathological records from 107 neonates in our hospital from January 1998 to May 2008 were collected,analyzed and summarized.Results 40.2%of the cases died within 24 hours and 72.9%died within 7 days after birth.The main death cause for those who died within 7 days was asphyxia(48.6%),and the main death causes ior those who died after 7 days were deformity(34.5%)and hemorrhage(34.5%).The most common death cause for premature bahies Was hyaline membrane disease(29.7%),and the most common one for term infants was congenital heart disease(23.3%).In the past 5 years.asphyxia Was still the most common death causein our country,those who died of infection were on the rise and the deformed babies increased too;on the contrary.those who died of obstetric causes and hemorrhage decreased.Conclusion Neonatal mortality could be reduced by prenatal management,prevention of premature delivery,improvement of the cooperation between pediatrics and obstetrics,and better management of NICU.

BACKGROUND: Hippocampal formation of brain, a cerebral area related with learning and memory, is closely related to spatial cognitive activity.Peroxidative stress following the onset of cerebral ischemia can induce DNA injury in hippocampal 'area and dentate gyrus, the fall of the ability of DNA plerosis and correspondingly a decline in the function of learning and memory.OBJECTIVE: To investigate the effect of hongjingtian on the expression of nucleic acid of hippocampal area and dentate gyrus, the learning and memory area of rats with complete cerebral ischemia-reperfusion injury.DESIGN: Randomized controlled study.SETTING: Central Laboratory of Armed Police Medical College.PARTICIPANTS: The experiment was completed at the Central Laboratory of Armed Police Medical College from April 2002 to April 2004. Totally 60 Wistar male rats were selected and divided randomly into 5 groups Model group: Rats were perfused daily with distilled water of a volume the perfused daily with distilled water of a volume the same as that in medication group.METHODS: Rats in each group were perfused incessantly for 7 days.Modified Pulsinelli-4 vessel blocking method was used to reproduce the rat model of acute complete cerebral ischemia-reperfusion. Rats in sham-operation group were not treated with the operation of burning vertebral artery and clipping common carotid artery. The changes of DNA and RNA expressions in cerebral hippocampal area and dentate gyrus were observed with acridine orange staining method after model establishment.MAIN OUTCOME MEASURES: Expression of DNA and RNA in cerebral hippocampal area and dentate gyrus of rats in each group.RESULTS: All 60 rats entered the final analysis. DNA and RNA in shamoperation group distributed evenly, border of fluorescent reflex was legible and response intensity was strong. Border of DNA and RNA fluorescent reflex was illegible and response intensity was obviously weak in model ly, border of fluorescent reflex was legible and response intensity was group was not of obvious difference as compared with that in model group.CONCLUSION: The illegibility of DNA and RNA fluorescent response in operation group is related with the breakage of DNA chain induced by oxidative stress during cerebral ischemia-reperfusion injury. Border of DNA and RNA fluorescent reflex in hippocampal area and dentate gyrus is legihibit the breakage of DNA chain induced by oxidative stress and protect learning and memory function in hippocampal area and dentate gyrus of rats with complete cerebral ischemia-reperfusion.

OBJECTIVE: To prepare ofloxacin liposomes for increasing bacteria sensitivity, and lo evaluate its in vitro properly. METHODS: Ofloxacin liposomes were prepared by pH gradient method using phospholipid and cholesterol as materials. The effects of pH value and the warming temperature and time on encapsulation efficiency were investigated. The particle size of liposomes was determined and the morphology was investigated by transmission electric microscope (TEM). The in vitro release experiment was carried out vising physiological saline as the medium. The bacteriostatic action against Staphylococcus aureus was investigated using disc diffusion test and dilution test. RESULTS: The maximum encapsulation efficiency of 82.40% could be achieved when pH value was 7.0, warming temperature was 50°C and warming time was 5 min. The average particle size of the liposomes was 174nm, and the ofloxacin liposomes showed good morphology under TEM. The liposomes could release ofloxacin in a sustained manner in vitro. The ofloxacin liposomes and solution showed same inhibition zones in disc diffusion test, while their minimal inhibitory concentrations determined by dilution test were 0.39 and 0.78μg · mL-1, respectively. CONCLUSION: Ofloxacin liposomes can be prepared by pH gradient method with high encapsulation efficiency, and the bacteriostatic effect of ofloxacin against Staphylococcus aureus can be improved by incorporating it into liposomes.