Objective To investigate the expression of RCAS1,which is a receptor-binding cancer antigen expressed on SiSo cells in many tumor cell lines. Methods Using real-time polymerase chain reaction(PCR),Northern blot,immunohistochemical examination and enzyme-linked immunosorbent assay,we detected the expre-(ssion) of RCAS1 in 10 different tumor cell lines and two normal cell lines. Results The expression of RCAS1 in the tumor cell lines and the fetal renal cell line(293 cell) was detected by real-time PCR and Northern blot,but not in the normal liver cell line(LO2 cell).The results of immunohistochemical examination on the tumor cell lines and the 293 cell showed that RCAS1 existed in cytoplasma and on cell surface.Except HuH-7 cell and LO2 cell,the soluble RCAS1 protein in supernatants of the other cells was significantly more than that in DMEM(P

Vascular Endothelial Growth Factor and its receptors may be the pivotal stimulants in the process of growth and metastasis of a neoplasm. They boost the genesis of the blood vessels of brain glioma. Their expression is correlated with the grading of glioma. They are ideal targets in the treatment of inhibiting the growth of the blood vessels of brain glioma.

To explore the opportunities and the challenges on continuing medical education of health technique cadres of armed police force,the article discussed the countermeasures of con-tinuing medical education of armed police force,in order to develop the work of continuing medical education of armed police force,which offered the methods and references for bringing up the ex-cellent health technique cadre of armed police force.

AIM: Using surface active agent that has wetting ability and solubilising to increase the extraction rate of curcumin. METHODS: Different surface active agents, such as span, span 60, tween 20, tween 85. SDS and CSBS, were added and then the curcumin was determined. RESULTS: When we added 0.5% sodium dodecyl sulfate (SDS), it could increase the extractive rate of curcumin by 16%. CONCLUSION: Different surface active agents have variable solubiitzation of different drugs. SDS can affect the extractive rate of curcumin.

Objective To investigate the in vitro effect of multi glycosidorum triptery (MGT) on cytokine production by splenocytes of oxazolone (OXZ) induced colitis in murine model. Methods Six mg of OXZ (in 50% of ethanol) was administered in male SJL/J mice intrarectally to induce colitis and mice were sacrificed 3 days later. Isolated splenocytes were cultured for 24 hours in the presence of PMA and ionomycin, MGT of 0.1 mg/ml or 0.01 mg/ml was added to the culture medium of splenocytes. Production of IFN ? and interleukin 4 (IL 4) in the supernatant was measured by ELISA. Results The production of IFN ? was suppressed by both 0.01 mg/ml and 0.1 mg/ml of MGT [normal control ( 1.24 ? 0.13 ) pg/ml→(0.97?0.26) pg/ml→(0.87?0.18) pg/ml, P

Objective To evaluate the myocardial protective effects of intracoronary(IC)adenosine infusion in patients with coronary heart disease during percutaneous coronary intervention(PCI).Methods A total of 111 enrolled patients were randomised to receive infusions of saline(control group,55 patients)or adenosine(adenosine group,56 patients).10 mL saline(control group)or 300 ?g adenosine(adenosine group)was given by intracoronary(IC)injection within 1 minute.The peak level of ST-segment elevation in IC electrocardiograms(IC-ECG)and the peak level of cardiac triponin I(cTnI)was analyzed and left ventricular ejection fraction(LVEF)was measured.For the patients with acute myocardial infarction(AMI),coronary flow of the infract-related artery was assessed by the method of thrombolysis in myocardial infarction grading.The peak level of sum ST-segment resolution(STR)was measured after one hour of PCI.Results The peak level of serum cTnI in adenosine group was lower than the control group(P

Objective To analyse the clinical manifestation of thoracostomach-airway fistula in order to improve the recognition.Methods The clinical information in 20 cases with thoracostomach-airway fistulas was analyzed retrospectively.Results The main clinical manifestations were choke after drinking or eating,inhalation pneumonia,fever,becoming thin ness,aridity,turbulence of water and electrolyte,et al.The stomach radiography showed thoracostomach-tracheal fistulas in six cases,thoracostomach-carina fistula in fwo cases,thoracostomach-left main bronchial fistulas in 10 cases and thoracostomach-right main bronchial fistula in two cases,vespectively.Conclusions Thoracostomach-airway fistula could be diagnosed according to the specific clinical significances and stomach radiography.

BACKGROUND: Dendritic cells play an important role in antigen present in vivo, and the mechanism of tumor cells in escaping the antigen presentation of dendritic cells existed in the patients with tumor.OBJECTIVE: To sensitize dendritic cells from human peripheral blood with apoptotic hepatoma cells induced by mitomycin.DESIGN: A randomized control trial by taking apoptotic hepatoma cell sensitized dendritic cells as the observed subjects.SETTINGS: Institute of Field Surgery, Daping Hospital, the Third Military Medical University of Chinese PLA; Institute of Radiation Medicine,Chinese PLA Academy of Military Medical Sciences.MATERIALS: The experiments were carried out in the Institute of Radiation Medicine, Chinese PLA Academy of Military Medical Sciences from April 1998 to May 1999. The cell strain was the QBC939 bile duct cancer cell strain, and mitomycin was used as the antitumor drug.METHODS: Mononuclear cells were isolated from the peripheral blood of normal people, 50μg/L granulocyte-macrophage colony stimulating factor(GM-CSF) and 1 000 U/mL interleukin-4 (IL-4) were added, once every other day for 4 times. On the 3rd day of culture, the apoptotic bile duct cancer cells induced by mitomycin was added, and then cultured in vitro for 4 days, finally the dendritic cells were collected.MAIN OUTCOME MEASURES: ① The identification of the cultured dendritic cells was observed; ② The dendritic cells were co-cultured with necrotic and normally cultured bile duct cancer cells respectively, and the phagocytized apoptotic body loaded antigens were observed; ③ The immunostimulatory activity of dendritic cells (1×103, 5×103 and 1×104/well)and that after loaded by antigen were detected, and the mononuclear cells were taken as controls.RESULTS: ① The cultured and amplified dendritic cells expressed high levels of costimulatory molecules of CD1a and B7, and there were typical irregular processes on the surface. ② The tumor cells formed apoptotic bodies when they were induced by mitomycin, which were arrested and phagocytized by dendritic cells. ③ The ability of the antigen loaded dendritic cells in stimulating the proliferation of allogenic lymphocyte T was further enhanced.CONCLUSION: The apoptotic tumor cells induced by mitomycin can induce the mononuclear cells from human peripheral blood differentiating into the dendritic cells with the concomitance of GM-CSF and recombinant IL-4 and amplify dendritic cells. Meanwhile, the dendritic cells can effectively present the antigens of apoptotic bile duct cancer cells, and it probably becomes a new effective approach for tumor antigen to sensitize dendritic cells.

Objective To study the clinical, imageological and pathologic characters of primary synovial chondromatosis of hip joint, and effect of open surgery. Method 12 cases of primary synovial chondromatosis of the hip joints admitted from May 1973 to May 2004 were retrospectively studied in respect to clinical manifestations, X-ray picture, and pathological findings. The effects of operation were followed up. Results Intermittent pain, swelling, limitation of hip joints were the main complaints. Mass and myatrophy near involved hip joint were discernible in a few patients. Although there was no obvious image of calcification, swelling of articular capsule was seen in X-ray film in 4 patients. CT scan showed arthroedema, loose bodies and bone erosion of the femoral head in the hip joint. The X-ray films of the other 8 patients showed obvious calcified loose bodies. All cases were diagnosed by pathology after operations. 8 cases were categorized Milgram Ⅱstage, and 4 cases Milgram Ⅲ stage. Eight patients were followed-up for a period of 1 to 7 years (mean 5 years). Recurrence occurred in 2 cases. Conclusion The diagnosis should be based on clinical characters, X-ray films and pathologic findings, and the pathology is most reliable. In order to decrease recurrence, CT or MRI examinations should be taken before the operation. X-ray monitoring should be carried out during the operation of the patient in whom loose bodies were found in X-ray film before the operation.

Objective To evaluate the feasibility and safety of percutaneous transluminal angioplasty(PTA)using ? 30 mm balloon catheter to dilate the obstructive lesion of inferior vena cava(IVC)in Budd-Chiari syndrome(BCS). Methods From January 2002 to July 2006,146 consecutive patients with BCS(94 men,52 women)underwent color Doppler sonography including enhanced multislice CT(n = 47),and were confirmed by IVC angiography. All patients but 1 microsomia case were treated by PTA using ? 30 mm balloon catheter for dilation. IVC angiography were immediately performed to show patency of IVC and occurrence of any rupture or not after PTA. Follow-up color Doppler sonography was taken to evaluate the IVC patency and the PTA-related complication about 1 week after PTA. Results The mean transversial and longitudinal diameters of IVC at the junction level of right atrium and IVC were(25.09 ? 6.09) mm and(17.57 ? 3.93) mmrespectively in 46 cases with 3 D multi-slice CT preoperatively(1 case without measurement was due to the closest site with right atrium). There were no IVC rupture during the procedure. Conclusions Treatment of BCS by PTA using ? 30 mm big balloon catheter is feasible and safe.