Objective To explore the relationship between N-acetyltransferase-2(NAT2)alleles polymorphism and type 2 diabetes mellitus(T2DM).Methods Polymerase chain reaction and sequence determination technique were used to identify NAT2 alleles in 174 patients with T2DM(T2DM group)and 174 unrelated healthy individuals(control group).Results Compared with control group,the allelic frequency of NAT2 mutation was no significant difference in T21DM group(x2=7.38,P＞0.05).The frequencies of the NAT2 genotypes(Wt/Wt,Wt/M1,Wt/M2,Wt/M3,M1/M1,M1/M2,M1/M3,M2/M2,M2/M3,M3/M3)were 39.08％(68/174),3.45％(6/174),22.99％(40/174),14.94％(26/174),0.57％(1/174),2.30％(4/174),1.15％(2/174),4.60％(8/174),9.20％(16/174),1.72％(3/174)and 44.83％(78/174),2.60％(8/174),27.59％(48/174),17.24％(30/174),0.57％(1/174),0.57％(1/174),0.57％(1/174),1.15％(21174),2.30％(4/174),0.57％(1/174)respectively,and significant differences showed between T2DM group and control group(x2=13.92,P＜0.01).The proportion of fast acetylate in T2DM group was significantly higher than that in control group[94.25％(164/174)vs.80.46％(140/174)],and the propoaion of slow acetylate in T2DM group was significantly lower than that in control group[5.75％(10/174)vs.19.54％(34/174)](P＜0.05),The risk of T2DM in people who were fast acetylate had 3.98 times(95％ CI:1.90-8.35)more than that in people who were slow acetylate.Conclusion It shows that the fast acetylate may be one of the genetic factors that influencing the susceptibility to T2DM,and slow acetylate may be one of the protecting factors.

AIM: To explore the effect of acute and chronic hypoxia on the chemotaxis of polymorphonuclear leukocytes(PMNs) of rats.METHODS: Serum and PMNs were separated from rats under normoxia, acute hypoxia and chronic hypoxia condition,and cell aspiration technology system was used to determine the chemotactic migration of pseudopod of PMNs induced by serum as chemotactic factor.RESULTS: Induced by autoserum,the length of pseudopod of PMNs migrated into micropipette [(1.200?0.178)?m,(1.094?0.164)?m ]under acute hypoxic and chronic hypoxic condition was significantly longer than the corresponding value for control[(0.914?0.156)?m,P

Treatment of sacral fractures,unstable ones in particular,has attracted more and more attention.Despite numerous classification systems for sacral fractures,the commonly used ones are Denis and Tile classifications.Stable sacral fractures deserve conservative treatment,but unstable ones should be treated surgically,by operative reduction,internal fixation to reconstruct stability of the pelvis,and prevention or relief of nerve compression.There are many methods of internal fixation for unstable sacral fractures,and each has its own advantages and disadvantages.This review summarizes the progress in surgical treatment of unstable sacral fractures and makes comments on the latest surgical strategies.

Adolescent ; Child ; Child, Preschool ; Female ; Homeodomain Proteins ; genetics ; Humans ; Infant ; Male ; Oncogene Proteins, Fusion ; genetics ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; genetics

Child ; Gene Rearrangement, T-Lymphocyte ; Humans ; Neoplasm, Residual ; diagnosis ; Polymerase Chain Reaction ; methods ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; diagnosis ; Receptors, Antigen, T-Cell ; genetics

0.05). The cell cycles of the two groups were similar, and heteroploid cells were not found out in both groups. CONCLUSION: Xenogeneic deproteinized cancellous bone has a good biocompatibility to bone mesenchymal stem cells and promotes cell growth and differentiation.

Objective To observe the influence of Edaravone on the level of serum gastrin and efficacy in patients with craniocerebral injury.Methods 60 patients with craniocerebral injury were randomly divided into Edaravone group(30 cases) and Dexamethasone group(30 cases).Then each group received corresponding treatment.The level of serum gastrin was detected at 1st d,3rd d,5th d and 7th d after treatment.The curative effect was evaluated based on the neuroligical deficit score(NDS) and Barthel index(BI) one month after treatment.Results The level of serum gastrin in Edaravone group began to decrease at the 3rd d after treatment,at the 5th d and 7th d became to normal value.While,in Dexamethasone group 5th d after treatment,the level of serum gastrin began to decrease,but at the 7th d was still significantly higher than the normal value.The levels of serum gastim at 3 d,5 d and 7 d after treatment were significant difference between the two groups(P

Objective To summarize the technique and evaluate the effect of anesthesia for off-pump coronary artery bypass surgery (OPCAB). Methods From 1997 through March of 2005, five hundred and forty-three consecutive patients undergoing OPCAB were retrospectively studied. Anesthesia was induced with midazolam 0.05～0.12mg/kg, ketamine 0～0.5mg/kg and fentanyl 5～15?g/?kg. Anesthesia was maintained with isoflurane 0.3%～1.5% and oxygen, combined with propofol 1～3mg/(kg?h). Intraoperative hypotension resulting from either surgical procedure or anesthetics should be dealt with inotropes immediately. Results Intraoperative hemodynamics deviation was tolerated with the support of inotropics. The mean extubation time was 5.8?1.6h. The ICU stay was 0.5 to 2.0 day. The patients were discharged in 8.0?1.2 days. Two patients met sudden death in the second postoperative day. Conclusion Rational use of anesthetics and inotropics to stabilize hemodynamics during operation plays a key role in successful OPCAB.

Objective To analyse the clinical manifestation of thoracostomach-airway fistula in order to improve the recognition.Methods The clinical information in 20 cases with thoracostomach-airway fistulas was analyzed retrospectively.Results The main clinical manifestations were choke after drinking or eating,inhalation pneumonia,fever,becoming thin ness,aridity,turbulence of water and electrolyte,et al.The stomach radiography showed thoracostomach-tracheal fistulas in six cases,thoracostomach-carina fistula in fwo cases,thoracostomach-left main bronchial fistulas in 10 cases and thoracostomach-right main bronchial fistula in two cases,vespectively.Conclusions Thoracostomach-airway fistula could be diagnosed according to the specific clinical significances and stomach radiography.

Objective To investigate the effects of hypoxia on the rheologic properties of leukocytes in microcirculation of rats, and its relationship with the expressive changes of CD18 of neutrophils. Methods Hypobaric chamber was used to simulate the plateau condition for the establishment of animal hypoxic model. Adult Wistar rats were randomized in three groups: control group, short-term hypoxia group (Hypobaric chamber simulated 5000m altitude for 3days) and long-term hypoxia group (Hypobaric chamber simulated 5000m altitude for 30 days). Intravital microscopy and computer image analysis system were used to evaluate the rheologic properties of leuckcytes in mesentery microcirculation. Neutrophils of the three experimental groups were purified and the expression of CD18 was determined by flow cytometer. Results Compared with control group, the rheologic properties of leukocytes were changed obviously at both short-term hypoxia and long-term hypoxia groups, including the number of leukocytes rolling and adherenced on endothelium increased, TLECT (total leukocyte-endothelium contact time) obviously extended (P