Objective To study the effect of CO 2 pneumoperitoneum and enteric disturbance on serum ?-endorphin (?-EP) in SD rats, in order to investigate their influence on peri-operative stress responses in SD rats. Methods A total of 120 SPF-grade male SD rats were randomly divided into four groups with 30 rats in each group. The four groups received CO 2 pneumoperitoneum (Group A), a 5 cm abdominal incision (Group B), a 5 cm abdominal incision with gastroenteric disturbance (Group C), and intraperitoneal anesthesia (Group D or Control Group), respectively. Concentrations of serum ?-EP of these groups were measured 10 min, 20 min, and 40 min after the beginning of surgery, respectively. Results Concentrations of serum ?-EP in the Group A 10 min, 20 min, and 40 min after the beginning of surgery were 274 7?66 6 pg/dl, 157 3?63 8 pg/dl, and 163 9?74 5 pg/dl, respectively, which were all extremely significantly higher than those in the Control Group ( P

Cerebral Infarction ; drug therapy ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Humans ; Phytotherapy ; methods

Objective To analyse and conclude the MRI features of intracranial germ cell tumours so that to improve the diagnosis of this disease.Methods 15 cases of germ cell tumours verified by operation and pathology were reviewed and analyzed. All cases were undergone plain and contrast-enhanced MRI,the fat restrained sequence was performed if necessary. Results The location of tumors,six were in pineal region, six were in sellar region (germinoma, teratoma and choriocarcinoma in pineal and sellar region were all separately 3, 2 and 1), two were in basal ganglia (germinoma), and one was in right lateral ventricle (teratoma).Conclusion MRI is very valuable in the diagnosis and differential diagnosis of intracranial germ cell tumours.

The heptadecatungstodiphosphate substituted by L a(Ⅲ) with the formula　α2-K7P2W17O61(La.OH2 ) (P2W 17La) was prepared by vacant refilling method. The anion of single substitu ted Dawson-type tungstophosphate salt was doped into the polypyrrole (PPY) film on a glassy carbon electrode (GC) by electrochemical method to prepare the chem ically modified electrode (P2W17La/PPY/GC). It maintains not only t he electrochemical activity and electrocatalytic property of P2W17L a, but also has good stability and sensitivity. In 0.50 mol/L HCl solution, th e first cathodic peak of P2W17La doping in polypyrrole film can cat alyze well the electroreduction of nitrite. The catalytic current was proporti onal to the concentration of nitrite between 6.0×10-5 and 1.0×10-2 　mol/L.

Objective To study protein variation between PtenL/LMEFs and Pten△/△MEFs cells.Methods Two-dimensional electrophoresis(2-DE) was employed to compare the differential expression proteins between PtenL/LMEFs and Pten△/△MEFs cells.Six differential expression proteins were digested in gel by enzyme and the mass of generated peptides was measured by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS).The data obtained from peptide mass fingerprinting(PMF) were searched using the Internet available database and five proteins were identified.Results Compared with that of PtenL/LMEFs,expression level of proteins including phosphoglycerate mutase 1(PGAM1) and peptidyl-prolyl cis-trans isomerase C(PPIC) was up-regulated,whereas expression level of Transgelin 2 was down-regulated in Pten△/△MEFs cells.B and F proteins were both identified to be peroxiredoxin-6.They had similar molecular weight but different PI which might be caused by post-translation modification.B protein was only expressed in Pten△/△MEFs cells.Conclusion The protein profile of Pten△/△MEFs cells displayed obvious difference compared to that of PtenL/LMEFs cells.The results implied that various distinct different proteins might lead to cancer.

Objective:To observe the clinical efficacy and safety of compound Bletilla Striata cream in the treatment of patients with second degree hand rhagadia. Methods: Totally 146 patients with second degree hand rhagadia were randomly divided into the treatment group and the control group with 73 cases in each. The treatment group was treated with compound Bletilla Striata cream, while the control group was treated with urea ointment. The treatment course was 10 days. The time of paln disappearance, keratoder-ma softening and rhagadia healing were compared between the two groups, and the efficacy and adverse drug reactions of the two groups were also observed and compared. Results:After the first treatment course, the curative rate of the treatment group was 94. 5%, which was higher than that of the control group (82. 2%) with statistical significance (P<0. 05). The total effective rate of the two groups was both 100% without statistical significance (P>0. 05). The time of keratoderma softening and rhagadia healing in the treatment group was significant shorter than that in the control group (P<0. 05), while the time of paln disappearance showed no significant difference between the two groups(P>0. 05). Conclusion: Compound Bletilla Striata cream in the treatment of second degree hand rhagadia is effective and safe, which shows good prospect for clinical application.

Objective To observe the effects of the Dachaihu decoction on intra-abdominal pressure in severe acute pancreatitis. Methods 70 patients with SAP from China-Japan Friendship Hospital were randomly divided into two groups. 35 patients in a conventional treatment group were treated with conventional treatment of severe acute pancreatitis, and 35 patients in a Dachaihu decoction treatment group were treated with the decoction through nasogastric tube based on conventional treatment. The changes of intra-abdominal pressure and the morbidity of IAH and ACS in both groups were observed in acute stage. Results The intra-abdominal pressure in both groups increased. But the pressure in the Dachaihu decoction treatment group (13.31 ± 4.42 mmHg, 13.02 ± 5.23 mmHg, 12.35 ± 3.34 mmHg, 11.26 ± 4.46 mmHg, 9.89 ± 3.44 mmHg) was lower than the conventional treatment group (16.89 ± 5.71 mmHg, 17.52 ± 3.37 mmHg, 16.21 ± 2.15 mmHg, 14.57 ± 5.56 mmHg, 12.11 ± 1.28 mmHg) from the third day on (P<0.05). And the morbidity of intra-abdominal hypertension and abdominal compartment syndrome in the Dachaihu decoction treatment group were lower than the conventional treatment group in the whole acut stage (40%vs. 8.75%).The differences between two groups were statistically significant (P<0.05). Conclusion The Dachaihu decoction may significantly reduce the intra-abdominal pressure and the morbidity of intra-abdominal hypertension and abdominal compartment syndrome among patients with severe acute pancreatitis.

Obesity is an independent risk factor for acute pancreatitis (AP),and the morbidity risk and severity of AP in obese patients were significantly increased than those in non-obese patients.However,the exact mechanism has not been fully elucidated.In recent years,the researches on the role of adipose tissue (AT) in AP have gradually attracted wide attention of scholars both at home and abroad.To better understand the pathogenesis and new therapeutic targets of AP,in this paper,we overviewed the recent research progress on the role of AT in AP.

Objective:To investigate the clinical effect of endoscopic sinus surgery in the treatment of sinusitis and nasal polyps and its effect on nasal ventilation and olfactory function.Methods:80 patients with sinusitis and nasal polyps were selected in our hospital from January 2014 to June 2016,who were randomly divided into observation group and control group,each group of 40 cases.The control group was treated with traditional enucleation,while the observation group was treated with endoscopic sinus surgery.The clinical efficacy,the symptom scores,nasal airway resistance and olfactory function score before treatment and 3 months after treatment were compared between the two groups.Results:The total effective rate of the observation group was 95.00％,which was significantly higher than that of the control group 77.50％ (P＜0.05).There was no significant difference in nasal congestion,purulent nasal discharge,olfactory dysfunction,pain and total symptom score between the two groups before treatment (P＞0.05),nasal congestion,purulent nasal discharge,olfactory dysfunction,pain and total symptom score between the two groups at 3 months after treatment were lower than before treatment,and nasal pus,smell disorder,pain and total symptom scores of the patients in the observation group were lower than the control group (P＜0.05).There was no significant difference in nasal airway resistance and olfactory function score between the two groups before treatment and 3 months after treatment (P＞0.05),nasal airway resistance,olfactory function score of patients in two groups at 3 months after treatment were lower than before treatment,and the observation group was lower than that ofthe control group (P＜0.05).Conclusion:Endoscopic sinus surgery for sinusitis and nasal polyps is helpful to improve the clinical symptoms,promote the olfactory function and recovery of nasal ventilation,which is an effective method for treatment of sinusitis and nasal polyps.

Acinar cell cystadenoma (ACA),also referred to as acinar cystic transformation,is a rare and newly recognized cystic lesion of the pancreas.This cystic lesion displaying apparent acinar cell differentiation and lacking of distinct cellular atypia was accepted as the benign counterpart of acinar cystadenocarcinoma.However,the exact etiopathogenesis of ACA is still not clear.