Objective:To evaluate the effect of NF-? B inhibitor-PDTC on TNF-? induced cytotoxicity in breast cancer cells.Methods:The cell survivals of breast cancer cell lines MCF-7 and MDA-MB-435s were measured by MTT assay in vitro.MDA-MB-435s cells were implanted into BALB/C mice. Animal tumor models were treated with TNF-?, PDTC and TNF-? plus PDTC.Results:The growth of two breast cancer cell lines was not inhibited by either TNF-?(2000U/mi or less)or PDTC.However,both breast cancer cells treated with TNF-?(2000U/mi)plus PDTC(50? mol)showed significant growth inhibition as compared with controls. In vivo, the growth of implanted tumor was significantly reduced in volume in mice treated with TNF-?(7.5?10 6U/kg,ip,bid) plus PDTC(50mg/kg,ip,bid) as compared with controls( P

Objective:To investigate the antiinflammatory effect of Chai Ge Oral Liquid.Methods:The antiinflammatory effect on mice feet swelling caused by carrageena and ear swelling caused by xylene was observed.Results:The inflammation of mice feet and ear swelling apparently was refrained by Chai Ge Oral Liquid,The median effective dose(ED 50 ) was 0.41ml/kg.Conclusion:Chai Ge Oral Liquid has antiinflammatory effect.

Objective:To observe the effect of hepatic function in hyperthyroidism patients with serum levels of hyaluronic acid(HA),laminin,type Ⅲ procollagen peptide and type IV collagen.Methods:We measured them in 140 patients with Graves' disease and 33 healthy subjects by radioimmunoassay.Graves' disease patients were divided into two groups,one including 59 patients with primary diagnosis and not treated with antithyroid drug(ATD),the other group including 81 patients were once treated with ATD.Results:HA concentration was significantly higher( P

Objective:To determine urinary N-acetyl-beta-d-glucosaminidase (NAG) and products of fibrin degradation (FDP) in patients with lupus nephritis and to discuss its significance.Methods:We measured the levels of urinary NAG,FDP in 36 cases of patients with lupus nephritis and 20 cases in the control group using auto-biochemical-analyzer,colorimetry and latex coagulation test.Results:The levels of urinary NAG, FDP in patients with lupus nephritis with normal renal function were significantly higher than those in the control group.The levels of urinary NAG,FDP in patients with lupus nephritis with abnormal renal function were significantly higher than those normal ones.The levels of urinary NAG with lupus nephritis were significantly higher than those with negative FDP in the urine.Conclusion:The change of urinary NAG in the lupus nephritis patients suggests that the damage in renal tubular interstitum has taken place in LN patients when they are on the renal sufficiency stage.With the development of the coagulation in the glomeruli,the degree of renal tubular interstitum damage in LN also increases.

Objective:To explore the clinical features of perimesencephalic nonaneurysmal subarachnoid hemorrhage (PNSH).Methods:The clinical manifestation,CT,treatment and prognosis of eighteen cases were analyzed.Results:All patients were conscious,whose Hunt-Hess scale were Grade Ⅰ to Ⅱ.In 18 cases headache developed,15 (83.3%) vomiting,14(77.8%)meningeal irritation,and 2(11.1%) focal neurologic deficits.All patients recovered completely.Conclusion:PNSH is a distinct clinical entity with few manifestations,good prognosis and low rate of severe complications.Repeated angiography should be avoided for PNSH.

Objective:To evaluate the effectiveness of intratumoral and intraperitoneal injection with retrovirus HSVtk gene on mouse bladder cancer.Methods:Subcutaneous and intraperitoneal bladder tumor was respectively established with mouse cancer cell line(T739) in syngeneic mice.Direct intratumor injection of retroviral HSVtk into the subcutaneous tumor was performed.In peritoneum cavity tumor model,HSVtk gene was transferred by intraperitoneal injection of retrovirus vector carrying HSVtk gene.After GCV therapy the tumor volume and animal survival time were monitored.Results:Both animal models were successfully induced in all mice within one week following subcutaneous and intraperitoneal injection of T739 cells.Compared with control animals,the tumor growth in both models was significantly inhibited with HSVtk/GCV system,and also the animal survival time was prolonged with administration of GCV(P

Objective:To explore the changes of serum leptin in chronic congestive heart failure(CHF).Methods:The serum levels of leptin in 46 patients with NYHA cardiac function class Ⅰ and in 39 patients with CHF(14 in class Ⅱ,21 in class Ⅲ and 4 in class Ⅳ)were assessed by radioimmunoassay.Results:The serum contents of leptin were 11.674?6.911?g/l in NYHA class Ⅰ group,11.492?5.649 ?g/l in classⅡ group,7.763?3.321 ?g/l in class Ⅲ group,6.100? 2.657 ?g/l in class Ⅳ group and 9.018?4.519 ?g/l in CHF group.The serum levels of leptin were significantly lower in class Ⅲ group,class Ⅳ group and CHF group,compared with class Ⅰ group,respectively( t =2.185～2.786, P

Objective:To evaluate the feasibility of argon laser-induced choroidal neovascularization(CNV)model in the pigmental rabbits in order to establish the foundation for studying the mechanism of CNV and for the development of treatment for CNV.Methods:Experimental eyes in 20 rabbits were received a series of 20 argon green laser(514.5nm) lesions per eye (700 mW,50um,0.1s).Fundus fluorescein angiography(FFA) and indocyanine green angiography (ICGA) were performed,then the rabbits were sacrificed immediately,the eyes were enucleated and processed for histopathologic and immunohistochemistry examination on days 3,7,14,21,30.Results:CNV first appeared on day 7 after photocoagulation,reaching the peak on day 21.The incidence of CNV was 63.0%.Disciform leakage staining appeared in the FFA.ICGA showed that the disciform area was filled with CNV.CNV was ascertained by light microscopy.The immunohistochemistry examination of VEGF was positive in ganglion cell layer and inner nuclear layer of retina from 2w after photocoagulation.Conclusion:The present studies demonstrated that argon laser photocoagulation could be used to produce choroidal neovascularization experimental model in the pigmental rabbits.CNV can be induced rapidly by the method,persists for a long period and at a high successful rate.VEGF plays a role in the formation of CNV.

Objective:To investigate the expression and significance of an inhibitor gene of apoptosis,Clusterin,in prostate cancer,and its relationship with the expression of bcl-2,FasL.Methods:The expression of Clusterin,bcl-2 and FasL in sample of 10 normal prostate,15 BPH and 49 prostate cancer were examined by immunohistochemical stain.Results:The rate of positive or weak positive for normal prostate,BPH and prostate cancer was 10%(1/10),66.6%(10/15)and 91.8%(45/49) respectively.The expression level of Clusterin in prostate cancer was much higher than in normal prostate(P

Objective: To study predictive factors of chronic hydrocephalus among patients with aneurysmal subarachnoid hemorrhage. Methods;Thirty two patients with aneurysmal subarachnoid hemorrhage were retrospectively studied. Results;The morbidities of chronic hydrocephalus between patients were more than 60 years old and less than 60 years old, Hunt-Hess grade III - V and I -II , posterior circulation aneurisms and anterior communicating artery aneurisms and the other aneurisms, time of bleeding were significantly different statistically ( P