1.The compared study of laparoscopic and open surgery efficiency for kidney cyst
Mongolian Medical Sciences 2016;178(4):34-37
Backgroundin 2009 we have been done the laparoscopic nephrectomy in the patient with left kidney cancer. In Urologyand Andrological center of State central hospital we have been done the urological laparoscopic surgerywhich is 0.32% of all surgery from 2010 to 2016. Following such increasing number of laparoscopicsurgery in urology we aimed to study surgical approach’s efficiency between open and laparoscopic forkidney cyst.Material and MethodsSince 2012 in surgery for kidney cysts studies case series models the center UNTE urology andandrology center based on clinical cases 34. Renal cysts with her open and laparoscopic surgicaladmissions bed days, wound size, surgery time, the average number of parameters such as size of thecyst value, standard deviation, median values, the high and low values expressed are compared.ResultIn our study includes 34 patients who was undergo the kidney cystectomy by open or laparoscopictechnique and ages between 18-68 (average age 48.1±12.07). 20 of them female and 14 of them malepatients. 18 of them were undergone laparoscopic cystectomy and 16 were undergone open surgery.Our study show that the cyst size is 2.5-10cm (average size 6±1.7) and there is no statistical correlationbetween cyst size and surgical types. The laparoscopic kidney cystectomy procedure lasts around 55-130min (average duration 93.1±23) which is 26 min longer than open cystectomy. The patient who wentto laparoscopic surgery their hospital staying duration is 2 fold less than the patient who went to opensurgery. Post operation surgical wound long is 4±0.8cm in laparoscopic surgery and 20.1±2.8 in opensurgery and therefore 5 times less in laparoscopic surgeryConclusionEven though the laparascopic kidney cystectomy’s procedure duration is longer than open technique, ithas less hospital staying duration, small surgical wound and fast rehabilation time.
2.Study of polymorphisms in autosomal microsatellite loci among mongolian population
Uyanga G ; Sarantuya J ; Purevdulam SH ; Ganbold S
Mongolian Medical Sciences 2013;165(3):7-15
IndroductionThe short tandem repeats (STR) are rich source of highly polymorphic markers in the human genome. In this study, we used a commercially available multiplex STR typing kit to study 15 STR systems (D3S1358, THO1, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, vWA, D8S1179, TPOX and FGA,) in the Mongolians population, and estimated the allele and genotype frequencies. These 15 STR loci include 2 new pentanucleotide repeat STR loci, Penta E and Penta D, so are not studied in Mongolians.GoalTo determine allele frequency of STR loci D3S1358, THO1,D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX, FGA Penta E, Penta D in Mongolian population.Materials and MethodsThe liquid blood, blood stain and saliva samples were taken from 165 unrelated individuals from Mongolian. Extraction DNA: Genomic DNA was extracted from whole blood samples by the standard method of phenol-chloroform-isoamyl alcohol and Wizard Genomic DNA Purification kit, Promega Corporation [21], from blood stain and saliva samples QIAamp DNA micro kit, Qiagen [25], AccuPrep Genomic DNA Extration kit, Bioneer, Koreans extraction method respectively.PCR: PCR amplification was performed using 10-15 ng genomic DNA template according to manufacturer’s protocol (PowerPlex® 16 and PowerPlex® 16HS kit, Promega Corporation, USA). Typing: DNA typing was performed on the ABI Prism 310 Genetic Analyzer (Applied Biosystems) using the recommended protocol. The results were analyzed by Data Collection (Version 1.1), GeneScan (Version 3.1), and Genotyper (Version 3.1) softwares (AppliedBiosystems).ResultsWe assessed forensic and population genetic studies using 15 STR loci included in s sample of 165 unrelated individuals from Mongolian. Allele frequency were listed in Table 2. Totally 20 alleles /5, 7-25/ were found from microsatellite Penta E locus and allele 11 has most frequent (0.1128). 6-16 alleles were found from Penta D locus and allele 9 has most frequent (0.3262). This result is interesting because allele 6 of Penta D locus was found rarely among other populations. But relatively higher frequency of allele 6 (0.0183) was found in Mongolian population. A population comparison based in genetic distance and genetic diversity calculated from allele frequencies of the 15 STR loci from obtained five different populations is shown the Table 3. Conclusions:1. Penta E locus was highly polymorphic, and 20 alleles were found in this Mongolians population and allele 11 was most frequent.2. Penta D locus was 20 alleles were found in this Mongolians population and allele 9 was most frequent.
3.The history of robot used in the urology and future trends
Ganbold G ; Bayan-Undur D ; Nyamsuren D
Mongolian Medical Sciences 2021;195(1):70-79
However, the use of robots in medicine has only 30 years of history. The application of robots in
surgery originates from the need of modern man to achieve two goals: the telepresence and the
performance of repetitive and accurate tasks. The first ‘‘robot surgeon’’ used on a human patient
was the PUMA 200 in 1985. In the 1990s, scientists developed the concept of ‘‘master–slave’’ robot,
which consisted of a robot with remote manipulators controlled by a surgeon at a surgical workstation.
Despite the lack of force and tactile feedback, technical advantages of robotic surgery, such as 3D
vision, stable and magnified image, Endo Wrist instruments, physiologic tremor filtering, and motion
scaling, have been considered fundamental to overcome many of the limitations of the laparoscopic
surgery. Since the approval of the da Vinci robot by international agencies, American, European, and
Asian surgeons have proved its factibility and safety for the performance of many different robot-assisted surgeries.
Comparative studies of robotic and laparoscopic surgical procedures in general surgery have shown
similar results with regard to perioperative, oncological, and functional outcomes. However, higher
costs and lack of haptic feedback represent the major limitations of current robotic technology to
become the standard technique of minimally invasive surgery worldwide. Therefore, the future of
robotic surgery involves cost reduction, development of new platforms and technologies, creation
and validation of curriculum and virtual simulators, and conduction of randomized clinical trials to
determine the best applications of robotics.
4.Minimally Invasive Approaches to Ureteropelvic Junction Obstruction
Ganbold G ; Bayan-Undur D ; Nyamsuren D ; Baasanjav N
Mongolian Medical Sciences 2019;190(4):52-59
There are many treatment options for the management of ureteropelvic junction obstruction (UPJO).
Open pyeloplasty has a high success rate and has been considered as a gold standard. Minimally
invasive surgical techniques are associated with reduced morbidity, improved cosmetic result and
better convalescence than open pyeloplasty. For endopyelotomy, these advantages for minimally
invasive surgery such as laparoscopic pyeloplasty and robot assisted pyeloplasty have superior
success rate than open pyeloplasty. However, the success rate for laparoscopic surgery could
potentially be improved by careful selection of patients, using the criteria of stricture <2 cm, renal
function >25% and the absence of severe hydronephrosis. Laparoscopic pyeloplasty and robot-assisted pyeloplasty have similar success rates to open pyeloplasty (>90%) and the best outcomes
have been reported for robot-assisted pyeloplasty although this treatment option is less readily
available than laparoscopic pyeloplasty. Retrograde endopyelotomy is a simple, safe, and effective
therapeutic option for primary and secondary symptomatic UPJO.
Retrograde endopyelotomy should be considered a viable first-line treatment option for the
management of patients with UPJO. These include balloon dilation, antegrade endopyelotomy,
retrograde endopyelotomy, Acucise endopyelotomy and laparoscopic pyeloplasty. During last decade,
advances in endourological techniques have resulted in significant progress in the development of
minimally invasive surgical procedures to treat UPJO.
Surgeons described their modification of Kusters dismembered procedure that involved anastomosis
of the spatulated ureter to a projection of the lower aspect of the pelvis after a redundant portion
was excised. Laparoscopic pyeloplasty was first reported in 1993 both by Schuessler and others
and by Kavoussi and Peters, who utilized dismembered pyeloplasty technique. During last decade,
advances in endourological techniques have resulted in significant progress in the development
of minimally invasive surgical procedures to treat UPJO. The combination of less postoperative
morbidity, improved cosmesis, shorter convalescence and comparable operative success rates has
lured many patients away from gold standard of open pyeloplasty. Only few retrospective studies have
been conducted regarding laparoscopic versus open pyeloplasty. Success rates are comparable for
laparoscopic pyeloplasty.
The number of minimally invasive surgeries performed by us increased from year to year. Therefore
the characteristics and performance of the surgeries should be studied in detail and based on the
finding the evidence based medicine should be placed in.
5.Results of laparoscopic pyeloplasty for ureteropelvic junctional stricture
Ganbold G ; Bayan-Undur D ; Nyamsuren D ; Baasanjav N
Mongolian Medical Sciences 2020;194(4):10-16
Background:
An aim of this study was to evaluate the long-term functional outcomes of laparoscopic
ureteropyeloplasty compared to that of open surgery at the Urology and Andrology Center of the First
Central Hospital of Mongolia. Ureteropelvic junction (UPJ) is the most common site for upper urinary
tract obstruction occurring 1 in 750 - 1500 births. Laparoscopic pyeloplasty was first reported in 1993
by Schuessler WW and its technique was dismembered pyeloplasty.
Material and Methods:
In the period from June 2018 to September 2019, we have operated 91 ureteropyeloplasty
cases. Patients were randomized into Group I (45 laparoscopy) operated by the laparoscopic
ureteropyeloplasty and Group II (46 open surgery) operated by the open ureteropyeloplasty. All the
patients had ureteropelvic junction obstruction and ureteropyeloplasty was performed. Both groups
were compared according to the operative time, and recovery duration. We studied restoration of
renal function and causes of conditions. Demographic data including age, gender and complications
were recorded. Renal diethylenetriamine penta-acetate scintigraphy was respectively performed 6
months after surgery.
Results:
Mean age was 32±12.05 ranging 16-62 in all the study population. A total of 91 (55 men and 36
women) were participated. Ureteropelvic junction stricture was occurred 75.66% in laparoscopic
cases and 84.78% in open cases which leads to hydronephrosis and it was statistically different
(p<0.028). Compared to that of open surgery, wound size was 6 time smaller, blood loss and hospital
stay less than 2 fold and wound healing is 5 days shorter than open surgery. There was statistical
different (p<0.001) between parameters of 2 groups. In laparoscopic and open group respectively,
renal function was 41.78±10.02ml/min, 42.15±11.34 ml/min (1.73м2). After intervention, renal function
was increased by (46±10.17ml/min, 46.09±11.50ml/min) and there was difference between 2 groups
(p<0.003). In laparoscopic group, renal function was more improved than open group (p=0.05).
Conclusion
Laparoscopic surgery had less blood, less analgesics usage, fewer hospital stays, and faster wound
healing. Renal function was improved 6 months after surgery.
6. ASSESSMENT OF EXTRACORPOREAL SHOCK WAVE LITHOTRIPSY (ESWL) THERAPEUTIC EFFICIENCY IN MONGOLIA
Sarantsetseg N ; Nyambayar N ; Erdenesaikhan M ; Javkhlantugs D ; Myagmarsuren P ; Sodgerel B ; Ganbold G ; Ariunaa S ; Bayan-Undur D
Journal of Surgery 2016;20(2):42-45
Introduction: Extracorporeal shockwave lithotripsy (ESWL) revolutionizedthe treatment of urolithiasis and graduallybecame the favorite treatment option sothat today it is considered to be the first lineof treatment for patients with urolithiasis.The purpose of this study was assessment oftherapeutic efficacy, complications of ESWLin urolithiasis in Mongolia.Material and methods: A total of46 patients harboring renal and ureteralstones underwent ESWL between March2016 and September 2016 at First CentralHospital of Mongolia. Karl Storz ModulithSLK electromagnetic machines were usedto impart shock waves. All collected stonefragments sent for biochemical analysis.Results: A total of 46 patients 23 weremales (50%). Patients were mean age of34. The stone size distribution was 0.5cmto 3.1cm. The average treatment time wasranging from 75-110 minutes. The averagenumber of shock waves per treatmentwas 3172±378 (range 1500-4000). Theoverall success rate was 75.73%. All calculidisintegrated satisfactorily except for 3stones, which is located lower 1/3rd ofureter. Stone composition analysis proved tobe composed entirely or predominantly ofcalcium oxalate monohydrate. These patientsrequired to have ureterolithoextraction. Calculicomposition for remaining patients 12 werecalcium oxalate monohydrate, 17 calciumoxalate dehydrate, 6 uric acid and 1 struvite.Complications were mostly minor and rare.Most of the patients (90.7%) developedmacroscopic hematuria after treatment; fewpatients developed mild bruising at the entryand exit sites of the shockwaves on the bodywall. Severe complications such as renalhematoma and steinstrasse were diagnosedfor one patient each and their managementwas non-surgical.Conclusion: ESWL is therefore the firstline treatment for urolithiasis with stonesize smaller than 2cm. It has an efficiencyrate above 75, low procedure time, highsafety and good tolerability and minimalcomplication.
7.Evaluation the renal status of kidney donors in light of biomarcers and eGFR
Khulan P ; Narantuguldur D ; Naran G ; Bayan-Undur D ; Ganbold G ; Sarantsetseg G
Health Laboratory 2019;10(2):10-19
Purpose:
Follow-up examinations in kidney donors is an essential yet necessary process in organ transplantation. In this study, we aimed to evaluate kidney function using biomarkers and biomarker based eGFR in kidney donors within 5 years of organ transplantation.
Materials and method:
91 donors enrolled in our study. We measured body weight and blood and urine samples for laboratory tests. eGFR was calculated using 6 estimations.
Result:
The mean serum creatinine in participants was 0.81±0.22 mg/dL, cystatin C was 1.11±0.19 mg/dL, urea was 31.44±8.02 mg/L. Systolic hypertension in subjects was 130.0±16.5 mmHg while diastolic hypertension was 78.4±10.8 mmHg. In all donors, 15.9% (n=14) had hematuria, 23.6% (n=21) had proteinuria, 24.7% (n=19) had albuminuria. Body weight, creatinine, cystatin C and urea measurements had gradually increased over the years. The average eGFR was 72.9±17.9 to 112.8±34.0 ml/min/1.73m2 showing 0.15%-35.22% before donation. Follow – up rate was 28.3-59.2% of total donors.Having health insurance and living far from Ulaanbaatar city influenced follow – up rate. Donor registration data should be updated regularly.
Conclusion
1. Serum creatinine, cystatin C, urea was increasing in living kidney donors. Hypertension and microalbuminuria was greater than other donor study results.
2. eGFR decreased 0.15-35.22% in donor. CKD EPI combined equation was best for donor.
3. Health insurance and living far from Ulaanbaatar city were the influencing follow – up rate. Registration data is missing in 25.5%-82.4% of total donors suggesting enhancement in data collection.
8.The evaluation of immunosuppressive regimens in kidney transplant Mongolian recipients
Sarantsetseg J ; Byambadorj B ; Byambadash B ; Munkhjargal B ; Tumurbaatar B ; Jambaljav L ; Bayan-Undur D ; Ganbold L ; Chuluunbaatar D ; Oyunbileg B ; Batbaatar G ; Munkhbat B ; Nyamsuren D
Health Laboratory 2019;9(1):21-27
Background:
However kidney transplantation has being performed in Mongolia since
2006, because of pre-transplant sensitization, ABO incompatibility, hepatitis B and C virus
activation many patients are taken kidney transplantation in abroad. The transplantation
centers use own immunosuppressive regimens.
Objective:
Our aim was to assess the immunosuppressive regimens efficacy and toxicity
in kidney transplant Mongolian recipients.
Methods:
We analyzed data from 96 adult kidney transplant recipients who had taken
kidney transplantation in different transplant centers from August 2006 through January
2014. There were 3 kinds of regimens Group I Simulect induction with standard triple
/FK506/CyA+MMF/AZA+steroid/, Group II Campath-1H induction with CNI monotherapy
and Group III Campath-1H induction with standard triple /FK506/CyA+MMF/AZA+steroid/.
We retrospectively collected the post-transplant first two years serum creatinine. The study
was performed in 2014. The questionnaire was taken and blood samples collected for
determination of tacrolimus through level and for other laboratory tests. The primary end
point was the first two years serum creatinine, the secondary end points included rejection
episodes, blood through level of tacrolimus and some laboratory findings.
Results:
The post-transplant first two years serum creatinine levels were significantly
different in 3 groups. Group III showed similar results compared to Group I. There was not
enough data of biopsy proven acute rejection episodes however group II said more
rejections occurred. However participants said that rejection occurred in 15 (15.6%) biopsy
was done only 3 (3.1%) cases. Blood through level of tacrolimus was significantly different
in three groups. Some laboratory findings showed different between three groups.
Conclusions
A regimen of Campath-1H induction with CNI monotherapy (Group II) may
be advantageous for short-term renal function and cost effective but there were more
rejection complications and increased creatinine. The regimen of Campath-1H induction
11 with standard triple (Group III) may be advantageous for long-term renal function, allograft
survival, but there should consider about infection complications and polycythemia.
Simulect induction with standard triple could be best choice but transplantations were
performed in experienced centers. The study enrolled few cases and cases which were
performed at the beginning of transplant program so many things could influence on the
result. The study was compared beginner transplant center with experienced centers.
Longitudinal cohort study needed in the future.