Objective: To explore the effects of Lycium barbarum polysaccharide (LBP-X) on IL-2 and TNF-? gene expressions in human peripheral blood mononuclear cells. Methods: RT-PCR (reverse transcription-polymerase chain reaction) was used to detect the gene expressions of IL-2 and TNF-?. Results: LBP-X at 5-40 mg/L upregulated the levels of IL-2 and TNF-? mRNA in human peripheral blood mononuclear cells after optimal time culture. Conclusion: The immunoenhancing effect of Lycium barbarum polysaccharide may be associated with its stimulation of IL-2 and TNF-? gene expressions.

Objective: To study the antitumor effect of Lycium barbarum polysaccharide (LBP X) and its immunity regulating effect on S 180 bearing mice. Methods: S 180 bearing mice were used as animal model. The effect of LBP X on tumor weight and immune function was observed. Results: LBP X could inhibit the growth of S 180 effectively and increase thymus index, the macrophage phagocytic function, anti SRBC antibody secreted by the spleen cells, lymphocyte proliferation and CTL activity and decrease LPO markedly in S 180 bearing mice. Conclusion: The mechanism of tumor inhibition by LBP X was due to enhance the immune function.

For decades, coping has undergone intensive investigation through different theoretical lens. In this paper, three theoretical standings in coping research were proposed:namely,the process theory, the trait theory and the situation theory. Relevant research and measurements were introduced. In the end, different perspectives were reevaluated and implication for further research was discussed.

Objective: To explore the effect of perfectionism on depression using anxiety as a controllng variable,and to find possible personality moderators.Methods: 407 undergraduate students from 8 universities in Beijing and Xi'an completed a battery of questionnaires,including Frost Multidimensional Perfectionism Scale(FMPS),Beck Depression Inventory(BDI),Personality Adjective Checklist(PACL) and Self-rated Anxiety Scale(SAS).Results: Hierarchical Regression Analysis confirmed the effects of two negative perfectionism dimensions hesitating over actions and concerning over mistakes,on depression after controlling anxiety.The effect of organization on depression was moderated by the personality trait of cooperation.Conclusion: Dimensions of perfectionism have diverse effects on depression.Psychological intervention should be carried out according to individual characteristics.

Objective: To study the influencing factors of the rural medical service demand. Methods: The gravity model of medical service demand was derived based on Individual utility function and profit objective function of medical institutions, and the data regression examine model of some county-level and district-level hospital were used. Results: According to the research, the main factors influenced the quantity of medical services come from medical supplies. With the increase of resident disposable income, the elasticity of demand for medical services decreased, while visiting distance of elasticity of demand for medical services increased. Conclusion: To increase the accessibility of medical service, it needs to set meeting the resident’s personal demand as the center, to promote the development of private hospitals. The scale of large hospitals should be limited so as to lead large-scale hospitals to separate low value-added medical services and to promote effective implementation of the treatment classification system.

Amyotrophic lateral sclerosis (ALS) is a rare neurodegenerative disease characterized by progressive loss of upper and lower motor neuron. Insomnia is one of the widespread but underappreciated non-motor symptoms in ALS patients, which may not only seriously affect patients′ quality of life, but also reflect the lesions of central nervous system in ALS. This review summarizes the insomnia symptoms reported in current ALS studies, and summarizes the somatic factors and central nervous system changes associated with insomnia in ALS patients, so as to provide ideas for the development of effective intervention measures and future research.

Objective: To examine the reliability and validity of MBI-SS and discuss the academic characteristics that affect burnout. Methods: 353 undergraduate students were administrated MBI-SS and Academic Characteristic Survey. Results: Confirmatory factor analyses showed that the expected three-factor structure of the adapted versions of the MBI- Students Survey (including Exhaustion, Cynicism, and Reduced Efficacy) fitted to the data. Exploratory factor analyses showed that the Academic Characteristic Survey includes six dimensions. The result of Regression showed that 44.4% variance of student burnout can be explained by Academic Characteristics. Conclusion: Student burnout includes three dimensions: Exhaustion, Cynicism and Reduced efficacy. Academic self-efficacy,academic stress,relationship with teachers and relationship with fellow students are significant predictors for student burnout.

Objective: To study the Ross procedure in surgery for aortic valve disease. Methods: From October, 1994 to May, 2002, 7 patients underwent Ross operation for aortic valve diseases. Pulmonary homografts were used for reconstruction of right ventricular outflow tract (RVOT). Among them, male 4, female 3, age from 11 to 44 years old [average (28?10) years]. All had severe aortic valve regurgitation, 2 associated with moderate aortic valve stenosis, 1 with bicuspid aortic valve, 1 with atrium septal defect, and 1 with ventricular septal defect. Pulmonary valve function was normal in all patients. Results: There was no operative death. One patient died 3 years later of SBE. Echocardiography indicated the normal function of aortic and pulmonary valve and excellent heart function. Conclusion: Ross operation provides is difficult but yields excellent clinical results.

OBJECTIVE: Researches on total hip replacements are always the hot spots in recent years. The replacements have obtained satisfactory outcomes in clinic. However, the long-term effect is limited because of the material and technical problems. This paper is to summarize the developmental trend and the prospect of clinical application for total hip replacements in 10 years.DATA SOURCES: A computer-based online search of Medline database was undertaken to identify relevant articles published in English from January 1996 to January 2006 by using the keywords of "total hip replacements, surgical robot, tissue engineering, virtual endoscopy". Meanwhile,Chinese relevant articles published from January 1990 to December 2005 were searched in Wanfang database, the keywords were "total hip replacements, material, virtual endoscopy, surgical robot, tissue engineering" in Chinese.STUDY SELECTION: The articles were primarily checked, and the references in each literature were reviewed. Articles about the study on total hip replacements were involved; Meanwhile, the repetitive studies or Meta analysis were excluded.DATA EXTRACTION: Totally 39 relevant articles were collected, and 22 of them accorded with the. Inclusive criteria, 17 old or repetitive ones were excluded. Of the 22 articles, 5 were about the influence of artificial joint material on prosthetic loosening and its carcinogenesis; 2 about the microinvasion of joint replacement induced by computer-aided surgery navigation; 1 about the introduction of surgical robot; 3 about the development of custom-made and specially made artificial joint; 9 about the possibility for the surface replacement of hip; and 2 about the combination of tissue engineering technique and artificial joint.DATA SYNTHESIS: Modern researches on total hip replacements are exploring wearing tolerant prosthesis to reduce the prosthetic loosening caused by osteolysis and bone resorption. The computer-aided design/made technique is used for special design and manufacture according to the need of disease, so as to solve the fracture around prosthesis and meet the need of limb salvage surgery. The computer-aided surgery navigation realizes the microinvasion of joint replacement and the fast recovery after surgery. The successful rate of total hip replacements is improved by developing localized surgical robot in China. By using the virtual endoscopy, we can accurately know the damaged conditions of intraarticular cartilage and sub-cartilage bone, which are also able to be quantifized, so that more suitable and reliable prosthesis can be selected to increase the accuracy. The tissue engineering technique in repairing defect can increase the synostosis of prosthesis, and perfect the technique of joint replacement.CONCLUSION: In the coming 10 years, total hip replacements will develop towards stability, harmlessness, microinvasion, mechanization, individualization and diversification, so that the total hip replacements will be much stander and safer with higher successful rate.

Objective　To prepare monoclonal antibody (McAb) pool against Bacteroides fragilis and Clostridium perfringens and evaluated its effect in rapid diagnosis in surgical infection. Methods　After the preparation of monoclonal antibodies against B. fragilis and C. perfringens, 191 specimens collected from infectious patients was detected with indirect immunofluorescent antibody (IFA) and enzyme labeled antibody (ELA). The results were compared with routine cultural method (CM). Results　Among the 191 specimens, 53 (27.7%), 55 (28.8%) and 25 (13.1%)strains of B. fragilis were detected with IFA, ELA and CM respectively, and for C.perfringens, 12(6.3%), 11(5.8%), and 6(3.1%) strains were found. The detectable rate of anaerobic bacteria by IFA and ELA was higher than by CM, but no difference was found between these 2. Conclusion　The self-made McAb pool against B.fagilis and C.perfringens might be a rapid, specific, sensitive and simple McAb pool for detecting B.fragilis in surgical infection and C.perfringens in wartime.