Stathmin, a ubiquitously expressed cytosolic protein(Mr=19×10~3), is also called oncogene protein 18 (Op18). Stathmin is involved in the assembly of microtubule (MT) and spindle by binding the tubulin protein. It plays a key role in cell proliferation, differentiation, regeneration, and migration and has regulatory effects on the signal transduction. Recently, it is reported that stathmin is overexpressed in a variety of human malignancies. It induces tumor cell migration and invasion by regulating MT depolymeri-zation. Its post-translational modification influences the interaction with p53 protein and is involved in the initiation and progression of malignant tumor. Stathmin alone or in combination with chemotherapeutics has been used for tumor therapy. The internal association of stathmin with cancer etiology is still unknown. So, further studies are needed to determine the role of stathmin as potential tumor biomarker and a drug target in tumor therapy.

OBJECTIVE: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome. METHODS: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected. RESULTS: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05). CONCLUSION: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Objective Established the model of atherosclerosis (AS) cells.To explore Hydrogen sulfide donor (NaHS) in atherosclerosis model by oxidized low density lipoprotein (ox-LDL) induced of the NF-κBp65 factor expression.MethodsApplication concentration of 80ug/mL ox-LDL induced liver cancer cell HepG2 cells AS model, set up a control group and treatment group (10μmol/L、15μmol/L、30μmol/L、100μmol/L、500μmol/L、1000μmol/L), to observe hydrogen sulfide this cell model of the cell proliferation activity and the determination of TNF-α and IL-10 factors of the expression, through the RT-qPCR and Western-blot method for determining the expression of the NF-κBp65 expression situation.ResultsAt 24h the ox-LDL induced a large number of red-stained granules, free cholesterol and total cholesterol in HepG2 cells increased, and free cholesterol content was more than 50% of total cholesterol in HepG2 cells, indicating that AS cell model was established successfully.CCK8 method of cell proliferation activity test results showed that the NaHS in 30umol/L, duration of 48 h when the largest cell proliferation activity.The TNF-α cytokine by ELISA method for testing the results showed that the treatment group in 15umol/L and 30umol/L concentration compared with the control group decreased significantly (P<0.05), while the expression of IL-10 increased significantly (P<0.05), with statistical significance.RT-qPCR and Western-blot method results show that the NF-κBp65 factor expression significantly reduced compared with control group (P<0.05), with statistical significance.ConclusionH2S play a protective role in atherosclerotic lesions by reducing the NF-κBp65 factor expression, and provide a valuable reference for the development and clinical treatment of H2S donor-type drugs.

ObjectiveTo make known the role of family health worker in the prevention and treatment of chronic diseases.MethodsIn accordance with the inclusion criteria strictly,146 residents were recruited as samples by convenience sampling.These residents were trained with 《chronic disease family prevention guide》 provided by Beijing City Service Center,special topic discussion,peer education,and self-study.Family health worker certificate were granted for those qualified..SPSS software was used for data analysis.Results There was statistical significance,when the data was compared before and after the training program.ConclusionThe training program improved chronic disease prevention knowledge level on different extent.

Objective To explore the changes of the cardiac enzymes in the critical patients after operation and to analyze their relationship with the patient's condition level and the prognostic.Methods Fittyseven critical patients after operation were enrolled to observation group,and 55 small operation patients acted as control group.Their cardiac enzymes in three days after operation were detected and compared.Results The cardiac enzymes of the observation group increased significantly when compared with the control group(aspartate aminotransferase (AST) (153.1 ± 211.0) U/L vs (54.7 ± 39.0) U/L,t =3.404,P ＜ 0.01) ; lactic acid dehydrogenase(LDH) (381.0 ± 216.1) U/L vs (194.6 ± 75.3) U/L,t =6.050,P ＜ 0.01) ; creatine kinase (CK) (882.9 ±630.7) U/L vs (130.1 ± 71.8) U/L,t =8.796,P ＜0.01) ;creatinine kinase,MB isoenzyme (CK-MB)(314.3 ± 164.2) U/L vs (164.9 ± 87.7) U/L,t =5.979,P ＜ 0.01) ; α-hydroxybutyrate dehydrogenas(α-HBDH) (410.1 ± 327.6) U/L vs (27.8 ± 36.3) U/L,t =8.605,P ＜ 0.01) ; The fatality ratio was 24.6％ (14/57) in the observation group.The cardiac enzymes of the survival group existed significant difference compared with the death group(AST:(81.3 ± 40.5) U/L vs (373.6 ±342.1) U/L,t =5.585,P ＜0.01) ;LDH:(303.6±134.0) U/Lvs (618.6±250.1) U/L,t=6.064,P＜0.01) ;CK:(658.5±328.0)U/Lvs (1572.0±829.4) U/L,t =6.001 P＜0.01) ;CK-MB:(303.2±184.3) U/Lvs (738.6±445.6)U/L,t=5.242,P＜0.01) ;α-HBDH:(254.1±118.6) U/Lvs (499.3 ±147.6) U/L,t=6.320,P＜0.01).Conclusion Elevated myocardiac enzymes are common in the critical patients after operation.The change of the cardiac enzymes is related with the degree of disease and the wound.

Fifteen cases of malignant melanoma,17 cases of acquired nevus,18 cases of congenital small nevus,and 3 cases of congenital giant nevus were labeled with anti-human prolifera-tive cell antibody Ki-67 with ABC labeling method.It was found that 13 out of the 15 cases of malignant melanoma were positive and the positive rate of the tumor cells was ranged between 2% to 33%.After culture,the positive rate of the melanoma cells was 88%.But all the 3.types of melanocytic nevi were negative.The findings suggest that the labeling of anti-human proliferative cell antibody Ki-67 is a sensitive and specific method to identify melanocytic tumors and it is not sensitive enough to differentiate the minor changes of the 3 types of nevi.

To construct a cDNA subtractive library of genes transactivated by hepatitis C virus core protein with suppression subtractive hybridization technique. mRNA was isolated from HepG2 cells transfected with pcDNA3 1(-)-core and pcDNA3 1(-) empty vector,respectively, then cDNA was synthesized. After restriction enzyme RsaI digestion, small sized cDNA were obtained. Then tester cDNA was divided into two groups and ligated to the specific adaptor 1 and adaptor 2, respectively. After tester cDNA was hybridized with driver cDNA twice and underwent two times of nested PCR, and then it was subcloned into T/A plasmid vectors to set up the subtractive library. Amplification of the library was carried out with E.coli strain JM109. The cDNA were sequenced and analyzed in GenBank with Blast search after PCR. The subtractive library of genes transactivated by HCV core protein was constructed successfully. The amplified library contained 233 positive clones. Colony PCR product showed that 213 clones contained 100~ 1 000 bp inserts. Sequence analysis was performed in 63 clones. Six of the sequences were unknown genes before. The full length sequences were obtained with bioinformatics method,which had been accepted by GenBank. It suggested that six novel cDNA sequences might be target genes transactivated by HCV core protein.

There are increasing experimental evidences and clinical data showing that inflammatory reaction plays an important role in the production and development of atherosclerosis. Inflammatory process promotes the production and release of inflammatory factors by activation of monocytes and stimulation of endothelial cells, which stimulate the migration and proliferation of vascular smooth muscle cells. Hydrolases and cytokines are released from the activated and stimulated cells to increase local lesion and form atherosclerotic plaque with the development of inflammation. So, on the basis of the new pathogenesis of atherosclerosis,scientists try to interfere with atherosclerosis by anti-inflammatory pathway. This paper reviews the progress of drugs against atherosclerosis by the modulation of C reaction protein ,CD40-CD40L,infection and NF-?B.

AIM To observe the protective effect of quercetin on vascular endothelial cells injured by homocysteine in rabbits. METHODS The model of the vascular endothelial cells of rabbits injured by homocysteine was established; 50 mg?kg -1, 100 mg?kg -1 and 200 mg?kg -1 of quercetin were orally adminstered. After 30 days, the number of CEC, levels of MDA and NO,SOD activity in the serum were determined; ET concentration in the plasma was detected. The thoracic aorta was isolated to observe the vascular reactivity to nitroglycerin and ACh and tissue change. RESULTS The number of CEC, levels of MDA and ET were decreased and levels of SOD and NO were significantly increased in the quercetin-administered group compared with the injured group.Non-endothelium dependent relaxation of vessel caused by nitroglycerin was similar in all groups. Endothelium dependent relaxation caused by ACh was reduced in the injured group,whileas the relaxation trended to the normal degree in the quercetin-administered group. Pathological examination showed that quercetin was effective on the injured endothelial cells. CONCLUSION Quercetin produces protective effect on the injured vascular endothelial cells by homocysteine in rabbits.

Objective To investigate the value of video-assisted thoracoscopic surgery(VATS)in the treatment of coagulated hemothorax.Methods A prospective randomized controlled study.Between July 2005 and July 2007,62 patients with coagulated hemothorax were enrolled in this study.The patients were randomly divided into VATS and traditional thoracotomy groups(31 in each).The pathophysiological parameters of the two grups were compared.Results No significant difference in the sex,age,property and location of the wound,and rate of complicated injuries was noticed between the two groups.The mean operation time,duration of postoperative drainage,and postoperative hospital stay of the VATS group were significantly shorter than those in the traditional group [(52.1?24.4)min vs(120.2?47.2)min,t=-7.136,P=0.000;(1.7?0.7)d vs(4.8?1.8)d,t=-8.937,P=0.000;and(12.6?2.4)d vs(18.0?8.9)d,t=-3.262,P=0.002;respectively];and the mean intraoperative blood loss and the volume of thoracic drainage of the VATS group were significantly lower than those in the traditional group [(137.1?14.6)ml vs(203.2?53.4)ml,t=-6.648,P=0.000;and(181.3?37.9)ml vs(253.9?64.0)ml,t=-5.435,P=0.000,respectively].No significant difference in the rate of complications existed between the two groups(0 vs 1 case,?2=0.000,P=0.000).Conclusion Video-assisted thoracoscopy is feasible and safe for coagulated hemothorax and is worth being widely used.