Objective Established the model of atherosclerosis (AS) cells.To explore Hydrogen sulfide donor (NaHS) in atherosclerosis model by oxidized low density lipoprotein (ox-LDL) induced of the NF-κBp65 factor expression.MethodsApplication concentration of 80ug/mL ox-LDL induced liver cancer cell HepG2 cells AS model, set up a control group and treatment group (10μmol/L、15μmol/L、30μmol/L、100μmol/L、500μmol/L、1000μmol/L), to observe hydrogen sulfide this cell model of the cell proliferation activity and the determination of TNF-α and IL-10 factors of the expression, through the RT-qPCR and Western-blot method for determining the expression of the NF-κBp65 expression situation.ResultsAt 24h the ox-LDL induced a large number of red-stained granules, free cholesterol and total cholesterol in HepG2 cells increased, and free cholesterol content was more than 50% of total cholesterol in HepG2 cells, indicating that AS cell model was established successfully.CCK8 method of cell proliferation activity test results showed that the NaHS in 30umol/L, duration of 48 h when the largest cell proliferation activity.The TNF-α cytokine by ELISA method for testing the results showed that the treatment group in 15umol/L and 30umol/L concentration compared with the control group decreased significantly (P<0.05), while the expression of IL-10 increased significantly (P<0.05), with statistical significance.RT-qPCR and Western-blot method results show that the NF-κBp65 factor expression significantly reduced compared with control group (P<0.05), with statistical significance.ConclusionH2S play a protective role in atherosclerotic lesions by reducing the NF-κBp65 factor expression, and provide a valuable reference for the development and clinical treatment of H2S donor-type drugs.

ObjectiveTo make known the role of family health worker in the prevention and treatment of chronic diseases.MethodsIn accordance with the inclusion criteria strictly,146 residents were recruited as samples by convenience sampling.These residents were trained with 《chronic disease family prevention guide》 provided by Beijing City Service Center,special topic discussion,peer education,and self-study.Family health worker certificate were granted for those qualified..SPSS software was used for data analysis.Results There was statistical significance,when the data was compared before and after the training program.ConclusionThe training program improved chronic disease prevention knowledge level on different extent.

OBJECTIVE: To investigate the changes of neuropeptides content in a rat model of spleen-deficiency syndrome. METHODS: Rats were randomly divided into three groups: normal group, untreated group and treatment group. There were ten rats in each group. Spleen-deficiency syndrome was induced by intragastrically administration of rhubarb concentrate in rats of the untreated and treatment group. The rats in the treatment group were intragastrically administered with Sijunzi decoction, a traditional Chinese compound herbal medicine for strengthening spleen and supporting qi. Normal saline was intragastrically administered to the rats in the normal and untreated groups. The somatostatin (SS) and cholecystokinin-8 (CCK-8) content in hypothalamus and colons was detected by the method of radioimmunoassay and the D-xylose content in urine was also detected. RESULTS: The CCK-8 content in hypothalamus of the untreated group was higher than that of the normal group (P<0.05). SS and CCK-8 content in colons of the untreated group was higher than that of the normal group (P<0.05). The CCK-8 content in colons of the untreated group was lower than that of the treatment group (P<0.05). The excretion rate of D-xylose in the untreated group was lower than that of the normal group (P<0.05). CONCLUSION: The disorder of neuroendocrine regulation in rats with spleen-deficiency syndrome may be chiefly responsible for "spleen-deficency and dysfunction of spleen" in traditional Chinese medicine.

Stathmin, a ubiquitously expressed cytosolic protein(Mr=19×10~3), is also called oncogene protein 18 (Op18). Stathmin is involved in the assembly of microtubule (MT) and spindle by binding the tubulin protein. It plays a key role in cell proliferation, differentiation, regeneration, and migration and has regulatory effects on the signal transduction. Recently, it is reported that stathmin is overexpressed in a variety of human malignancies. It induces tumor cell migration and invasion by regulating MT depolymeri-zation. Its post-translational modification influences the interaction with p53 protein and is involved in the initiation and progression of malignant tumor. Stathmin alone or in combination with chemotherapeutics has been used for tumor therapy. The internal association of stathmin with cancer etiology is still unknown. So, further studies are needed to determine the role of stathmin as potential tumor biomarker and a drug target in tumor therapy.

Objective To summarize the nursing of infants with cavernous transformation of the portal vein (CTPV) and gastrointestinal bleeding undergoing distal splenorenal shunt.Methods 3 cases of infants with CTPV and gastrointestinal bleeding were given with preoperative nursing for upper gastrointestinal bleeding,strengthening nutritional support; strengthening prevention measures of thrombosis and bleeding complications,monitoring of urine and urine gravity,completing the artery piezometer tube care,paying attention to the discharge guidance and follow-up work.Results The platelet increased in 3 cases post-operation.1 cases occurred abdominal bleeding,but improved after symptomatic treatment.1 case occurred leakage on the site of venous indwelling needle during intravenous infusion of high nutrient solution.3 cases of children received 3 months to 3 years follow-up after discharge,no complications such as liver,kidney and blood clots occurred.Conclusions Infants with CTPV and gastrointestinal bleeding undergoing distal splenorenal shunt is very rare in clinic.Good perioperative care and strengthening the observation of the postoperative complications,attaching great importance to the discharge guidance and follow-up work,are the assurances of successful treatment.

BACKGROUND: Mental disorders of college students, especially medical college students, deteriorate year by year.Mental disorders can not only restrict healthy development of body and mind, but also cause social crisis.OBJECTIVE: To survey the prevalence of mental status and the correlation between mental status and family dynamics among medical college students.DESIGN: Randomized cluster sampling.SETTING: Center of Mental Health, the First Affiliated Hospital, Chongqing University of Medical Science.PARTICIPANTS: A total of 801 sophomores were selected from a medical university of Chongqing from November 2004 to June 2005.METHODS: ① Symptom Checklist-90 (SCL-90) was used to evaluate mental status among college students. If those students whose total scores were ≥ 200 and/or the score of any factor in SCL-90 was ≥ 3, they were regarded as having mental problem. The higher the scores were, the severer the mental problemwas. ② Family dynamics questionnaires, including family atmosphere, individuation, systemic logic and disease opinion, were used to evaluate characteristics of family dynamics. There were 4 items: family atmosphere, individuation, systemic logic and disease opinion. The more comfortable and cheerful the family atmosphere was, the lower the scores were; the greater the differentiated degrees were, the lower the scores of individuation were; the more objective the disposal of problems was,the lower the scores of systemic logic were; the more subjective the duty of recovery was, the lower the scores of disease opinion were.MAIN OUTCOME MEASURES: ① Prevalence of mental disorder; ② comparing scores of family dynamics between disorder group and normal group; ③ results of correlation analysis and step regression analysis between mental disorder and family dynamics.RESULTS: Among 801 questionnaires, 747 were involved in the final analysis. ① Prevalence of mental problem was 19.4%. ② The scores of family atmosphere, Individuation, systemic logic and disease opinion were all higher in disorder group than those in normal group, with significant differences (3.10±0.49, 2.66±0.64; 2.92±0.54, 2.77±0.58; 2.79±0.55,2.69±0.54; 2.58±0.67, 2.36±0.62, P ＜ 0.05). ③ The total score of SCL-90 was positive correlation with points of family atmosphere and disease opinion (r=0,184, 0,156, P ＜ 0.05). Family atmosphere, individuation and disease opinion were analyzed by regression equation.C0NCLUSION: Mental status of college students is closely related to family dynamics. Family atmosphere and disease opinion play a great role in mental disorder.

Objective To evaluate the impact of family intervention on the quality of life in the patients after total laryngectomy and their family members. Methods Family intervention was implemented for the patients after total laryngectomy and their family members from 2008 to 2009,and only patients received intervention from 2005 to 2007. The patients and their family members were investigated with Quality of Life Instrument for Cancer Patients-Head and Neck Cancer (QLICP-HN) and Quality of Life Scale-Family Version (QOL Scale-FAMILY) respectively when the patients were admitted to hospital and one year after discharged. Results The quality of life in patients after total laryngectomy and their family members was significantly improved after family intervention was implemented (P<0.05). Conclusion Family intervention is practicable and helpful to improve the quality of life in patients after total laryngectomy and their family members.

There are increasing experimental evidences and clinical data showing that inflammatory reaction plays an important role in the production and development of atherosclerosis. Inflammatory process promotes the production and release of inflammatory factors by activation of monocytes and stimulation of endothelial cells, which stimulate the migration and proliferation of vascular smooth muscle cells. Hydrolases and cytokines are released from the activated and stimulated cells to increase local lesion and form atherosclerotic plaque with the development of inflammation. So, on the basis of the new pathogenesis of atherosclerosis,scientists try to interfere with atherosclerosis by anti-inflammatory pathway. This paper reviews the progress of drugs against atherosclerosis by the modulation of C reaction protein ,CD40-CD40L,infection and NF-?B.

AIM To observe the protective effect of quercetin on vascular endothelial cells injured by homocysteine in rabbits. METHODS The model of the vascular endothelial cells of rabbits injured by homocysteine was established; 50 mg?kg -1, 100 mg?kg -1 and 200 mg?kg -1 of quercetin were orally adminstered. After 30 days, the number of CEC, levels of MDA and NO,SOD activity in the serum were determined; ET concentration in the plasma was detected. The thoracic aorta was isolated to observe the vascular reactivity to nitroglycerin and ACh and tissue change. RESULTS The number of CEC, levels of MDA and ET were decreased and levels of SOD and NO were significantly increased in the quercetin-administered group compared with the injured group.Non-endothelium dependent relaxation of vessel caused by nitroglycerin was similar in all groups. Endothelium dependent relaxation caused by ACh was reduced in the injured group,whileas the relaxation trended to the normal degree in the quercetin-administered group. Pathological examination showed that quercetin was effective on the injured endothelial cells. CONCLUSION Quercetin produces protective effect on the injured vascular endothelial cells by homocysteine in rabbits.

Objective To explore the effects of psychological health education on mental health status of middle school students. Methods All middle school students were randomly divided into 2 groups by cluster sampling, a interventional group and a control group. 28 students in the intervention group received group psychological consultation for two months, 30 students in the control group received no therapies.Changes of active respond and negative response subscales(ARNRS), communication anxiety scale (IAS),accommodate others scale(AOS), self-rating depression scale(SDS)were compared before and after the intervention in both groups. Results After group counseling, ARNRS, IAS, AOS, SDS in the interventional group was significantly changed compared with both pre-counseling and the control group, with P<0.05～0.0001 and P<0.05～0.0001, respectively. Conclusion Community mental health education can enhance middle-school student's psychological health level.