Objective To preliminary evaluate the efficacy and safety of Lev therapy in infant with epilepsy .Methods To chil-dren with infant epilepsy in the Department of Neurology of Chongqing Children′s Hospital from May 2010 to February 2012 were enrolled .All of them took Lev mono-therapy or adjunctive therapy .Investigation was done by self-controlled and retrospective re-search .There were 70 patients received Lev therapy ,aging from one month to one year old ,and 39 patients received mono-therapy , 31 patients received adjunctive therapy .All subjects were divided into 2 groups ,12 patients in partial seizure group and 58 patients in generalized seizure group .Then all patients took a 7 months Lev therapy after initial medication .The seizure frequency change , seizure situation ,side effect were observed .Results During the follow-up ,5 patients lost their follow-up(3 case in mono-therapy group ,2 case in adjunctive therapy group)and 3 patients died .In mono-therapy group ,83 .33% of patients got responder and 16 . 67% of patients unmodified or worsen ,while those in adjunctive therapy group were 58 .62% and 41 .38% ,respectively .And there was significant difference between two groups(P<0 .05) .In the partial seizure group ,81 .81% of patients got responder and the 18 .18% of patients unmodified or worsen ,while those in generalized seizure group were 70 .37% and 29 .63% .And there was no significant difference between partial and generalized seizure group(P>0 .05) .During the study ,22 patients ended Lev therapy for some reasons .77 .27% of patients discontinued in 1-6 months and 22 .73% of patients in 7-34 months .Number of the adverse reac-tions was 14 .Main adverse reactions observed were anorexia and sleep disorders in early .3(4 .61% )of 65 patients discontinued ther-apies for the adverse reaction ,but there was no death caused by adverse reaction .Conclusion The Lev mono-therapy and adjunctive therapy are both effective in infant with epilepsy ,and Lev therapy is effective in infant epilepsy .

OBJECTIVE:To provide practical standard for sampling the crude drugs.METHODS:To look up and analyse the relevent literature to the subject.RESULTS:The condition of sampling the cruds drugs for research reflected by literature is confused without standard given.CONCLUSION:It is a practical way to pay attention to the growing area,the channel to be obtained and authentication and analysis of crude drugs in research to control the quality.

Objective To investigate the effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-?. Methods Flow cytometry was used to analyze the apoptosis. The caspase-8 fluorescent kits were used to detect the activity of caspase-8. And the expression of caspase-8 enzymogen was examined by Western blot. Results Procyanidin (5, 10, and 20 mg/L) significantly inhibited the apoptosis of HeLa cells induced by rhTNF-? (100 ?g/L) (P

To study the changes in serum immunosuppressive acidic protein (IAP) and T lymphocytes rDNA transcription activity in peripheral blood of patients with pancreatic cancer before and after operation. The level of IAP and T lymphocytes rDNA transcription activity in peripheral blood of 58 patients with pancreatic cancer were measured before and after opreration by one directional immunodiffusion test and a cell image analysis of Ag NORs, respectively, and the results were compared with that of healthy controls. The level of IAP was higher while T lymphocytes rDNA transcription activity was lower before operation compared with healthy controls( P 0 05). The levels of IAP and T lymphocytes rDNA transcription activity were closely related to tumor invasion, metastasis and clinical stages (TNM). Serial determination of the levels of IAP and T lymphocytes rDNA transcription activity might serve as an important index for evalution of tumor invasion and metatasis, and also prognosis for the patients with pancreatic cancer

Objective To optimize the radioligand assays for glutamic acid decarboxylase antibody (GAD-Ab), tyrosine phosphatase antibody (IA-2A) and insulin autoantibody (IAA) and to facilitate their application in clinical practice. Methods The radioligand assays established in our laboratory were optimized in several aspects, including the mode and time of antigen-antibody incubation, pH value of TBST buffer, times of washing, and type of centrifuge. The sensitivity and specificity of the optimized assays were calculated by the Diabetes Antibody Standardization Program (DASP, 2003). And the results were compared to those measured with domestic radioimmune assay kits. Results The optimal condition for radiligand assay were as followed: incubating the antigens of GAD-Ab and IA-2A with serum by rotating slowly for 24 hours and IAA for 72 hours respectively; washing the precipitation complex of GAD-Ab and IA-2A with TBST buffer (pH value 7 2～7 4) 4 times and IAA 5 times respectively; and applying the horizontal type of centrifuge. The results from DASP demonstrated that sensitivity and specificity of the optimized assays were 82% and 98% respectively for GAD-Ab, and 64% and 100% for IA-2A. The proportion of consistency between our results for 82 IAA gradient index of optimized assay and those measured with domestic radioimmune kits was 89%. The extensive analysis showed that the consistency reached 100% for those with IAA index less than 0 04 or more than 1 00, and the inconsistency was for the bordline positive ones (index 0 04～1 0). Conclusion The optimized radioligand assays for islet autoantibodies are high sensitivity and specificity, and are applicable in clinical practice.

Objective To study the relationship between serum high sensitive C-reactive protein(Im-CRP) and acute coronary syndrome(ACS). Methods We detected and analyzed the levels of hs-CRP in 38 clinical deft-trite patients with ACS. Results Patients with ACS had significantly higher serum hs-CRP levels than the control group(P < 0.05). Conclusion Serum hs-CRP level has high correlation with ACS, and the hs-CRP maybe a arthero-sclerosis marker,which reflected the process of coronary artery disease.

Oral anti-viral agents nucleos (t)ide analogues (NAs) are widely used in treatment of chronic hepatitis B (CHB) since they are well-tolerated in most patients.But clinical relapse occurs in a considerable part of patients after the cessation of NAs therapy.This paper reviews factors related to clinical relapse, including time for drug withdrawal, cccDNA level, age, baseline alanine aminotransferase (ALT) and HBV DNA levels, type of NAs, serum level of HBsAg and the immune status of patients.

Objective To investigate the feasibility and clinical effect of hip joint arthrodesis with the application of Kirschner wire and reconstruction plate in the treatment of advanced tuberculosis in total hip joint.Methods A total of 30 patients with advanced tuberculosis of the hip in our hospital from March 2014 to April 2016 were divided as Kirschner wire group and reconstruction plate according to different surgical methods,with 15 cases in each group.The operation time,bleeding volume during operation (intraoperative and postoperative),fusion time,postoperative complications of two groups were observed and compared.Results Patients of two groups reached bony fusion.Compared with reconstruction plate group,Kirschner wire group had less blood loss,shorter operative time,longer fusion time,the differences were statistically significant (P ＜ 0.05).Conclusion Both Kirschner wire fixation and reconstruction plate arthrodesis are effective for patients with advanced tuberculosis of total hip joint,but the clinical efficacy of Kirschner wire fixation in hip arthrodesis was significantly better than the reconstruction plate.

OBJECTIVE: To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS). METHODS: The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively. RESULTS: PPS could elevate the iNOS activity with dose-dependence and stimulate the iNOS mRNA expression of peritoneal macrophages in mice. CONCLUSION: The regulation of PPS on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS. This indicates that the mechanism of PPS's antitumor and immunoregulation functions may be related to increasing NO output of macrophages through stimulating iNOS's denovo synthesis.