1. Effect of Beclin1 overexpression on biological behaviors of SK-MEL-2 human malignant melanoma cells
Yanyan XU ; JiuJiang WANG ; Ling ZHANG ; Zhao HAN ; Liang NIU ; Jianzhong ZHANG ; Zhao LIU ; Gaijing LI ; Xiaobing LI ; Qing LIU ; Zhijun LIU ; Xiaojing LI
Chinese Journal of Dermatology 2020;53(1):40-44
Objective:
To evaluate the effect of overexpression of the autophagy marker gene Beclin1 on biological behaviors of SK-MEL-2 human malignant melanoma cells.
Methods:
Western blot analysis was performed to determine the protein expression of Beclin1 in melanoma cell lines A375 and SK-MEL-2. SK-MEL-2 cells with low Beclin1 protein expression were selected as research objects, and divided into 3 groups: blank group receiving no treatment, negative control group transfected with pcDNA.3.1/myc-His (-) A, and experimental group transfected with pcDNA3.1-Beclin1 plasmid. After 2-week culture, cell counting kit-8 (CCK-8) assay was conducted to evaluate the effect of Beclin1 on cell proliferation at 24, 48 and 72 hours, and Transwell assay and wound-healing assay were performed to assess the effect of Beclin1 overexpression on the invasion and migration abilities of SK-MEL-2 cells. Repeated measures analysis of variance and completely randomized analysis of variance were used to analyze differences in indices among groups, and least significant difference (LSD) -