1.A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice.
Victor Abou NEHMI ; Gilson Masahiro MURATA ; Ruan Carlos Macêdo de MORAES ; Gabriely Cristina Alves LIMA ; Danielle Araujo DE MIRANDA ; Katrin RADLOFF ; Raquel Galvão Figuerêdo COSTA ; Joyce de Cassia Rosa de JESUS ; Jéssica Alves DE FREITAS ; Nayara Izabel VIANA ; Ruan PIMENTA ; Katia Ramos Moreira LEITE ; José Pinhata OTOCH ; Ana Flávia Marçal PESSOA
Journal of Integrative Medicine 2021;19(5):439-450
OBJECTIVE:
To evaluate the synergic effects of a novel oral supplement formulation, containing prebiotics, yeast β-glucans, minerals and silymarin (Silybum marianum), on lipid and glycidic metabolism, inflammatory and mitochondrial proteins of the liver, in control and high-fat diet-induced obese mice.
METHODS:
After an acclimation period, 32 male C57BL/6 mice were divided into the following groups: nonfat diet (NFD) vehicle, NFD supplemented, high-fat diet (HFD) vehicle and HFD supplemented. The vehicle and experimental formulation were administered orally by gavage once a day during the last four weeks of the diet (28 consecutive days). We then evaluated energy homeostasis, inflammation, and mitochondrial protein expression in these groups of mice.
RESULTS:
After four weeks of supplementation, study groups experienced reduced glycemia, dyslipidemia, fat, and hepatic fibrosis levels. Additionally, proliferator-activated receptor-α, AMP-activated protein kinase-1α, peroxisome proliferator-activated receptor γ co-activator-1α, and mitochondrial transcription factor A expression levels were augmented; however, levels of inhibitor of nuclear factor-κB kinase subunit α and p65 nuclear factor-κB expression, and oxidative markers were reduced. Notably, the cortisol/C-reactive protein ratio, a well-characterized marker of the hypothalamic-pituitary-adrenal axis immune interface status, was found to be modulated by the supplement.
CONCLUSION
We discovered that the novel supplement was able to modify different antioxidant, metabolic and inflammatory pathways, improving the energy homeostasis and inflammatory status, and consequently alleviated hepatic steatosis.
Animals
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Antioxidants
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Dietary Supplements
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Glucans
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Hypothalamo-Hypophyseal System
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Liver
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Mice
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Mice, Inbred C57BL
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Mice, Obese
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Milk Thistle
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Minerals
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Pituitary-Adrenal System
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Prebiotics
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Saccharomyces cerevisiae