Objective:To study the expression of cbf?1 on mouse MDPC-23 cell line,and discuss the role of cbf?1 in the regulation of dental embryo development. Methods:By transient transfection,immunofluorescence,and western blot,the expression of cbf?1 on mouse MDPC-23 cells were studied. Results:The control MDPC-23 cells didn't express cbf?1.After transient transfected for 48 h,cbf?l expression was significantlly increased.BMP_(2)could induce low level expression of cbf?1. Conclusion:cbf?l expression was significantly increased 48h after transient transfection.

Damaged articular cartilage has a limited intrinsic capacity to heal itself,especially in adults,It represents a clinical challenge.Novel gene therapy can introduce particular beneficial gene into the seeded sells and express growth factors or other therapy proteins at the repair site.Gene therapy focuses on selecting proper gene,target cells and the transferring systems. The tissue engineering cartilage with gene-modified seeding cells and transferring objective gene to target sells locally present new therapeutic regimens for repairing defects in articular cartilage.

Cartilage lesions resulting from acute or chronic injury are one of the major factors leading to joint disease and disability ,and eventually osteoarthritis. It is well known that articular cartilage in adults has a limited ability for self-repair,and represent a clinical management challenge. Numerous methods have been devised to augment its natural healing response. The most appropriate treatment option for an individual patient should be based on the pathologic characteristics of the lesion and the patient's symptoms, age and expectations. This review presents the current articular cartilage management and the direction in future therapeutic regimens.

Limb allotransplantation is an important procedure for reconstruction of limb defects.This review describes the history and development of this method,and expects to bring extremity transplantation closer to being a realistic possibility.

OBJECTIVETo study cellular and humoral immune status on prophase of severe hepatitis B (PSHB).

METHODS56 cases of PSHB patients, 40 cases of chronic hepatitis B (CHB) patients and 20 cases of healthy volunteers were enrolled for detection of CD3+, CD4+, CD8+ and CD3-/CD19+ (B cells) lymphocyte subsets in peripheral blood by flow cytometry. Serum IgG and complement C3 was detected by immunoturbidimetry and analyzed statistically.

RESULTSCompared with CHB group and healthy control group, percentage of lymphocyte subsets CD8+ were significantly lower in PSHB group (P < 0.01 or P < 0.05). While the percentage of lymphocyte subsets CD4+ and ratio of CD4+/CD8+ in PSHB group was obviously higher than those in CHB group (P < 0.+01 or P < 0.05). In addition, There was no significant difference on the percentage of B cell and level of serum IgG between PSHB group and CHB group (P > 0.05, while the level of serum complement C3 in PSHB group were significantly lower than those in CHB group and healthy control group (P < 0.01, P < 0.05).

CONCLUSIONPSHB has a certain degree of cellular immune dysfunction, which characterized by cellular immune function hyperfunction and humoral immune suppression.

Adult ; Antibodies, Viral ; immunology ; Complement C3 ; immunology ; Female ; Flow Cytometry ; Hepatitis B, Chronic ; immunology ; Humans ; Lymphocyte Count ; Male ; Middle Aged ; T-Lymphocyte Subsets ; cytology ; immunology ; Young Adult

The effect of Lycii Cortex on the PCOS rat model and the mechanism of action were investigated in the present study. The PCOS rat model was induced with Poretsky methods. Then the rats were randomly divided into four groups: the model group, melbine group (0.45 g x kg(-1)), low (2.5 g x kg(-1) and high (10 g x kg(-1)) dosage group of Lycii Cortex. The animals were orally administrated with the drugs for 14 days. In addition, another control group was added in this study. The rats were weighted before and after drug treatment. After 14 days treatment, oestrous cycle of rats were detected; blood serum was separated to determine T and FINS and rat's uteri were isolated. The mRNA and protein (total and phosphorylated) expressions of PI3K and PKB in uteri were measured with Real-time RT-PCR and Western blot, respectively. Compared with the control rats, the body weight gain and serum level of T and FINS were significantly increased. While, the mRNA and protein (phosphorylated) levels of PI3K and PKB were markedly decreased in PCOS group. Lycii Cortex treatment significantly decreased the body weight gain and serum level of T and FINS in a dose-dependant manner. It also markedly increased the mRNA and protein (phosphorylated) expressions of PI3K and PKB. Meanwhile, the melbine treatment also showed the curative effect. Lycii Cortex can relieve the symptoms of PCOS and the mechanism might be related to PI3K/PKB pathway.
Animals ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Humans ; Lycium ; chemistry ; Phosphatidylinositol 3-Kinases ; genetics ; metabolism ; Phosphorylation ; drug effects ; Polycystic Ovary Syndrome ; drug therapy ; enzymology ; genetics ; Proto-Oncogene Proteins c-akt ; genetics ; metabolism ; Rats ; Rats, Sprague-Dawley ; Signal Transduction ; drug effects

The stress resistance of Saccharomyces cerevisiae industrial strains for molasses to high-concentration ethanol,high temperature,high osmotic pressure,furfural toxicity,phenol toxicity,acetic acid toxicity and G418 toxicity were analyzed by the spot dilution growth assays in this paper. The results showed that the stress resistances among these industrial strains were obviously different. The strains AS2.1189 and AS2.1190 are more resistant to the tested stress factors than any others .The strain 396 is the most resistant to the acetic acid toxicity and G418 toxicity,and the strain 2610 is the most resistant to the high temperature.

Objective: To observe effects of different sulfonylurea compounds on expression of sulfonylurea receptor 2 (SUR2) in myocardium of the Goto-Kakizaki (GK) rats. Methods: Spontaneous diabetic GK rat models were divided into 6 groups: the diabetes model group, the Glibenclamide group, the Glipizide group, the Gliclazide group, the Glimepiride group and the positive control group(treated with insulin), with 12 rats in each group. A normal control group was also set up for comparison. The expression of SUR2 in myocardium of the GK rats was investigated by radioligand binding assay. SUR2 mRNA expression in the myocardial cells of rats was detected through reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Twelve weeks later, no significant difference was found in the SUR2 receptor density(Bmax)and affinity(Kd) between the sulfonylurea treated groups and the other 3 groups (P>0.05). There was no significant difference in SUR2 mRNA expression between the diabetic, insulin-treated diabetic and control groups(P>0.05). Cardiac SUR2 mRNA levels were not significantly different between sulfonylureas-treated diabetic and non-treated diabetic rats (P>0.05). Conclusion: The diabetes itself does not affect the sulfonylurea receptor(SUR2) expression in myocardial tissues. Sulfonylureas at treatment dosage have no effect on receptor expression of SUR2.

Anti-Inflammatory Agents ; pharmacology ; Child, Preschool ; Humans ; Infant ; Infant, Newborn ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; complications ; pathology ; Prednisone ; pharmacology

Animals ; Antibodies, Monoclonal ; metabolism ; therapeutic use ; Biomarkers, Tumor ; metabolism ; Breast Neoplasms ; metabolism ; pathology ; therapy ; Drug Delivery Systems ; Epidermal Growth Factor ; immunology ; metabolism ; physiology ; Epithelial-Mesenchymal Transition ; GPI-Linked Proteins ; Humans ; Immunotherapy ; methods ; Intercellular Signaling Peptides and Proteins ; Membrane Glycoproteins ; immunology ; metabolism ; physiology ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Proteins ; immunology ; metabolism ; physiology