Objective: This study aims to understand the awareness, attitude and parent-child communication of child sexual abuse (CSA) prevention among mothers of children with hearing loss. Methods: Data was collected using anonymous self-administered questionnaires among 188 mothers of children with hearing loss in 7 hearing rehabilitation institutes in Beijing, during April to June in 2018. Results: Mothers of children with hearing loss who participated in this study lacked knowledge. Only 3 participants (1.6%) could correctly answer all the 10 items. Besides, 71 participants (37.8%) held positive or supportive attitude towards all the 6 items of CSA prevention education. Most mothers thought CSA prevention education could help to prevent CSA, agreed with school-based relative education, and permitted their children to attend education activities. In addition, the participants lacked parent-child communication of CSA prevention, for only 12 mothers (6.4%) had talked with children about all the topics of the 10 items. Knowledge of mothers with high school or higher education was better than those with middle school or lower education(β=0.98, P<0.01). Scores of communication were significantly higher among mothers of elder children(β=0.33, P<0.01). Conclusion Further intervention should promote awareness and parent-child communication about CSA prevention among mothers of children with hearing loss.