Objective: To compare the effects on glucose tolerance and insulin sensitivity between equal isocaloric consumption moderate intensity continuous exercise and high-intensity intermittent exercise in healthy male adolescents, and to provide a reference for a reasonable exercise intervention program to maintain and improve the health level of adolescents. Methods: The 13 subjects from middle schools in Nantong were divided into control group, moderate intensity continuous exercise group (MICE) and high-intensity intermittent exercise group HIIE. The oral glucose tolerance tests were carried out on 13 healthy subjects in all conditions. Meanwhile, the insulin sensitivity index (ISI), total area under the curve (tAUC) and incremental area under the curve (iAUC) of glucose as well as insulin were calculated. Results: The tAUC of plasma glucose of MICE and HIIE group decreased by 5.99% (ES=-0.75) and 9.32% (ES=-0.86)(P<0.05) compared to C group, respectively. The tAUC of plasma glucose in HIIE group decreased by 3.54% (ES=-0.30, P<0.01) compared to MICE group. The tAUC of plasma insulin in MICE and HIIE group was attenuated 4.49% (ES=-0.64, P<0.01) and 6.79% (ES=-0.80, P<0.01) compared to C group, respectively. The tAUC of plasma glucose in HIIE group decreased by 2.42% (ES=-0.30，P<0.01) compared to MICE group. The iAUC of plasma glucose in MICE and HIIE group decreased by 25.52% (ES=-0.57, P<0.01) and 35.82% (ES=-0.73, P<0.01) compared to C group, respectively. The iAUC of plasma glucose in HIIE group decreased by 13.83% (ES=-0.16, P<0.05) compared to MICE group. The iAUC of plasma insulin in MICE and HIIE group descended by 7.14% (ES=-0.67, P<0.01) and 10.18%(ES=-0.75, P<0.01) compared to C group, respectively. The iAUC of plasma insulin in HIIE group decreased by 3.27% (ES=0.21, P<0.05) compared to MICE group. The ISI of MICE and HIIE group increased by 1.65% (ES=0.15, P>0.05) and 4.72% (ES=0.25, P<0.01) compared to C group, respectively. There was no significantly difference in ISI between MICE and HIIE group. Conclusion The intervention effects on the level of glucose, insulin and insulin sensitivity of high intensity intermittent exercise is better than moderate intensity continuous exercise among adolescents.