Anti-Infective Agents ; therapeutic use ; Humans ; Sinusitis ; drug therapy

Based on the theory of tumor angiogenesis,the vascular system is necessary for the advance and metastasis of the tumor.To inhibit or even cure the tumor,the researchers all over the world have made a great many of studies on the mechanism of tumor angiogenesis and have acquired some achievements.Based on the findings,a lot of factors are involved in tumor angiogenesis,and they constitute the complex regulating network through interaction at the molecular and cellular level.

China ; Chronic Disease ; Humans ; Phenotype ; Rhinitis ; genetics ; immunology ; Sinusitis ; genetics ; immunology

Asthma ; immunology ; Humans ; Rhinitis ; immunology

Allergens ; immunology ; Child ; Desensitization, Immunologic ; Humans ; Rhinitis, Allergic, Perennial ; immunology ; therapy ; Treatment Outcome

Objective: This study aims to investigate the protein expressions of cyclooxygenase-2 (Cox-2) and hypoxia-inducible factor 1 alpha (HIF-1α) and their correlations with the clinicopathological features of osteosarcoma and tries to reveal the potential mechanisms for the formation and progression of osteosarcoma. Methods: We screened 59 typical samples of osteosarcoma tissues without os and decalcification treatment. They were grouped according to the clinicopathologic characteristics and were followed up post surgery. Expression of Cox-2 and HIF-1 alpha were detected by immunohistochemistry. The results were evaluated by X2 analysis and Spearman rank correlation analysis. The postoperative survival rates were calculated by Kaplan-Meier survival curve and the differences between groups were analyzed by Log-Rank method. Results: The positive expression rates of COX-2 and HIF-1α in osteosarcoma were 69.49% and 38.98%, respectively, which were significantly different compared with negative expression in control group (P<0.01 and 0.05). The expression of Cox-2 in osteosarcoma positively correlated with the clinical stage (P<0.05) and the expression of HIF-1α correlated with the age of patients (P<0.01). The expressions of Cox-2 and HIF-1α were closely associated with the histological classifications. In COX-2- and HIF-1α-positive groups, the total positive rates (16.67% and 0%) of intraosseous well-differentiated and parosteaosteosarcoma were significantly lower than that of any others (73.33%, and 43.18%), respectively. The difference was significant (and P<0.01 and P<0.05). The expression of Cox-2 had positive correlation with HIF-1alpha. The positive co-expression of both HIF-1α and Cox-2 was 38.98%. There was 30.50% osteosarcoma tissues had no expression of both Cox-2 and HIF-1 alpha. The positive expression rates of HIF-1α in Cox-2 - positive and Cox-2 - negative groups were 56.10% and 0%, respectively. The difference was significant (P<0.01). The postoperative survival period was negatively associated with the expression of Cox-2 and HIF-1α. The postoperative accumulative survival rates were significantly lower in Cox-2 - and HIF-1 alpha - positive patients compared with those in Cox-2 - and HIF-1 alpha-negative patients (P<0.05 and P<0.01, respectively). Conclusion: Cox-2 and HIF-1α were over-expressed in osteosarcoma. Their expressions are related with the formation and progression of tumor and may have synergistic effects in prompting the malignant development of osteosarcoma. The patients with positive expression of Cox-2 and HIF-1α have lower postoperative accumulative survival rates. Their expressions may be useful prognostic factors for osteosarcoma.

Asthma ; immunology ; Congresses as Topic ; Humans ; Hypersensitivity ; immunology ; United States

Objective To investigate the value of intermediated cutting approach in the total thyroidectomy.Methods The clinical data of patients with total thyroidectomy from January 2009 to December 2011 were analyzed retrospectively.Patients in group A(n =134) were underwent thyroidectomy through intermediate approach,whereas patients in Group B (n =187) were underwent traditional total thyroidectomy.The analysis index included operation periods,operative bleeding amount,the incidence of hypocalcemia and rate of laryngeal nerve injure.Results The operation periods in Group A was shorter than that in group B ((65.4 ± 8.7) second vs.(90.1 ± 11.3) min,t =22.195,P ＜ 0.01).The amount of operative bleeding in group A was,less than that in group B ((20.5 ± 5.7) ml vs.(50.8 ± 11.4) ml,t =31.294,P ＜ 0.01).The incidence rates of temporary recurrent laryngeal nerve injure and the temporary hypocalcemia rate in group A and group B were (0.74％ vs.5.88％,10.44％ vs.24.59％ respectively,x2 =5.72,10.28 respectively,P ＜ 0.05).No permanent hypoparathyroidism or permanent recurrent laryngeal nerve injure case occurred in both groups.Conclusion Intermediate cutting approach was an effective and safe method in terms of total thyroidectomy.

Objective To describe the special ramp lesion, which involves the capsulomeniscal region of posterior horn of medial meniscus and most commonly occurs in association with anterior cruciate ligament(ACL) injury, pre- and intra-operative diagnosis methods, arthroscopy repair technique and follow-up results of arthroscopy second-look & MR review. Methods From April 2002 to April 2005, 89 cases of ACL injury with ramp lesions are enrolled in this series, among them 85 need operation repairing. Arthroscopic ACL reconstruction with hamstring or B-PT-B autograft or allograft and all-inside medial meniscus repair through 2 posteromedial portals and transcondylar portal have been performed. Generally, the ramp lesion accounts for 26.7% of ACL injury, 30.5% for chronic and 19.5% for acute injury respectively. Results Of the repaired ramp lesion cases, 75 cases(88.2%) are available for follow-up, with the duration from 5 to 41 months (mean, 20.2 months). 25 cases underwent arthroscopic second-look and 21 cases received MR evaluation. The study include subjective symptom, clinical examination, and arthroscopic second-look as well as MR review. All of the repaired cases present normal subjective and objective evaluations, and all the 25 second-look cases show completely healing. Of the 21 MR follow-up cases, 18 completely healed, 3 partial healed. Conclusion About 1/3 ACL injuries are associated with medial meniscus ramp lesion, and the morbidity is higher in old injuries than acute injuries. For this combined lesion, one stage ACL reconstruction and meniscus repair was recommended. All-inside meniscus repair is a satisfactory technique for ramp lesion and can get high healing rate.

Objective:To comparatively observe the effects of 20(R)-ginsenoside Rg3 and PEG-PLGA-Rg3 nanoparticles on the Lewis lung cancer mice and to explore the mechanisms of Rg3 and PEG-PLGA-Rg3 nanoparticle anti-cancer in vivo.Methods:Lewis lung cancer mouse model was established and 60 mice were randomly divided into 5 groups with twelve in each group:PEG-PLGA-Rg3 nanoparticles group (Rg3-N),PEG-PLGA group (PEG),Rg3 group (Rg3 ),normal control group (C),saline control group(NS),and received intragastric administration for 1 4 days.The weights of the mice were measured every 2 days and the weight curves were obtained.At the same time,the color pattern,activity and men-tal status were observed.The mice were sacrificed when the administration was over,and the effects of 20 (R)-ginsenoside Rg3 and PEG-PLGA-Rg3 nanoparticles on tumor weight,and the tumor:weight ratios were analysed.In addition,the tumor microvessel density (MVD)was measured by immunohistochemi-cal staining with anti-CD31 antibody to compare the effects of Rg3 and PEG-PLGA-Rg3 nanoparticles on the tumor angiogenesis in vivo.Furthermore,the levels of such angiogenesis and proliferation factors as MMP-9,HIF-1 α,VEGF,Ki-67 were examined by RT-PCR,Western blot and immunohistochemistry to explore the internal molecular mechanisms of anti-tumor effects in vivo.Results:The trends of variation of the mice weights in NS group and PEG group were rising early but declining later.In contrast,the trends of the other three groups were rising early and became stable later.In comparison with NS group, the mice of Rg3 group and Rg3-N group had better general status:brighter color,more active and better spirit.Compared with NS group,the tumor weight in PEG group,Rg3 group and Rg3-N group showed no significant difference but the tumor:weight ratio and MVD in Rg3 group and Rg3-N group declined signi-ficantly (P <0.01 ).Besides,there was no significant difference between Rg3 group and Rg3-N group. At the same time,the level of VEGF mRNA,the protein expression of MMP-9,HIF-1 α,VEGF in Rg3 group and Rg3-N group decreased compared with NS group.Furthermore,the level of each index above-mentioned in Rg3-N group was lower than that in Rg3 group.The expression of Ki-67 in PEG group,Rg3 group and Rg3-N group showed no significant difference compared with NS group.Conclusion:Rg3 and PEG-PLGA-Rg3 nanoparticle may suppress the expression of VEGF,MMP-9 and HIF-1 αin Lewis lung cancer mice,thereby indirectly contributing to their antitumor effects and alleviating the mice’s general status.In addition,PEG-PLGA nanoparticles embedding can promote Rg3 antitumor effect in vivo.