Objective:To investigate effects of dendritic cells(DCs) pulsed with autologous hepatoma cell lysates on the immune function of the postoperative patients with hepatocellular carcinoma(HCC).Methods:DCs isolated from peripheral blood mononuclear cell of HCC patients were cultured and proliferated in vitro by using rhGM-CSF and rhIL-4,and then were pulsed with autologous hepatoma cell lysates.30 postoperative patients with HCC were randomly divided into 2 groups.15 cases were treated with dendritic cell vaccine.15 cases received only chemotherapy and were established as control group.Immune function、clinical effects in two groups of patients were compared and observed.Results: The levels of DC3~+、DC4~+/CD8~+ and NK cell in the DC vaccine group significantly increased after vaccination,while those of the control group had no significant changes.The levels of IgG、IgM、IgA in the DC vaccine group increased after vaccination,but didn't reach statistical significance.The concentration of IL-10 in the DC vaccine group significantly decreased after vaccination(P

Interferons(IFNs) are a family of glycoproteins with antiviral, antitumor, and immunomodulatory activities, classi-fied into type Ⅰ, Ⅱ, and Ⅲ based on structural and receptor specificity. Among these, type Ⅲ IFNs(IFNλs), including IFNλ1(IL-29),IFNλ2(IL-28a),andIFNλ3(IL-28b),exhibittissue-specificreceptordistribution,predominantlyexpressedinepithelialcells and select immune cells, thereby demonstrating reduced systemic side effects compared to typeⅠIFNs. Mechanistically,IFNλs exert their antiviral effects through JAK/STAT signaling pathway inducing the expression of interferon-stimulatedgenes(ISGs), and inhibit tumor cell proliferation, promote apoptosis and regulate immune responses. Recent advances inrecombinant IFNλs therapeutic development include polyethylene glycol(PEG) modified IFNλ1, a longacting formulationcurrently in phaseⅢclinical trials for hepatitis C, hepatitis D, and COVID-19. In this paper, the structures and receptors,signaling pathway and regulation, biological functions and research progress of IFNλs are summarized in order to provideinsights for the development of IFNλs related products.
10.13200/j.cnki.cjb.004486

Fatty Liver ; Humans

Alcoholism ; complications ; Antiviral Agents ; therapeutic use ; Fatty Liver ; diagnosis ; epidemiology ; etiology ; therapy ; Hepatitis, Viral, Human ; complications ; diagnosis ; drug therapy ; Humans ; Insulin Resistance ; Interferons ; therapeutic use ; Obesity ; complications ; Risk Factors

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; prevention & control ; Humans ; Phytotherapy

Fatty Liver ; diagnosis ; epidemiology ; Prognosis

The new,rapid,accurate and convenient tests for tuberculosis diagnosis and drug resistance are urgently needed for effective tuberculosis control.In this review,two novel,inexpensive diagnosis methods of active TB were introduced.They can detect tuberculosis disease and drug resistances directly form sputum specimens and might have greater applicability in developing countries.Several concerns about the research and development of innovative diagnosis technology in our country also are described.

This paper presents a portable neurogenic bladder curer,which can produce a series of pulses according to human's physiology characteristic to stimulate bladder's points.Such bladder-related diseases can benefit from this curer as increased residual urine in the bladder,urine retention and urine incontinence.Being low-cost,small and portable,it can satisfy the needs of different patients.

In the course of the establishment of the basic medical insurance system, medical and health institutions at various levels are going to face the readjustment of functional orientation and transformation of the current pattern of medical setups will produce a far reaching effect on medical institutions. While “reducing burdens”, big hospitals must try actively to suit the needs of the medical market under the new circumstances, give full play to their strong points, such as high concentration of talents, knowledge and resources, and provide quality, high efficiency and high value added medical service. It must also be brought to peoples awareness that third tier hospitals are not just the supplement and extension of community medical service but should also serve as its guarantee and backing.

Purpose:To explore the in vitro effects of apoptosis induction of 2-methoxyestradiol(2ME2),an estrogen derivative on primary myeloma cells and hematopoietic precursors from multiple myeloma patients. Methods:The primary myeloma cells and hematopoietic precursors from 14 multiple myeloma patients were examined determine apoptosis cells in situ by terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL)assay. Results:After co-cultured with different concentrations (1,4,16?mol/L) 2 ME2 for 48h respectively,apoptotic ratios of myeloma cells were 10.5? 1.2%,21.2?2.3%,36.8?3.6% respectively ,which shows a concentration- dependence.In comparision with the control group,the difference is of statistical significance(P0.05). Conclusions:2ME2 could electively induce apoptosis of primary myeloma cells from myeloma patients and has little apoptotic effect on granulocytic,erythrocytic and lymphocytic cells in the bone marrow.