Objective:To approach the change of five serum markers such as CA15-3、CA125、CEA、CYFRA21-1 and TPS with breast cancer between pre-and post-neoadjuvant chemotherapy,to screen the better treatment effectiveness evaluation markers.Methods:The serum markers such as CA15-3、CA125、CEA、CYFRA21-1 and TPS of 48 cases with breast cancer were detected by enzyme linked immunosorbent assay respectively pre-and post-neoadjuvant chemotherapy.Results:In effective group,CA15-3、CYFRA21-1 and CEA were significantly decreased(P0.05).Through Logistic multiple factor regression analysis,CA15-3?CYFRA21-1and CEA were the better markers of among the five markers to evaluate the neoadjuvant chemotherapy efficacy of breast cancer,and evaluate role from strong to poor by turns.Conclusion:CA15-3 was the best marker of five serum markers to evaluate the neoadjuvant chemotherapy efficacy of breast cancer.

The results suggest that the root cause of formation of peritoneal adhesions is cong-cllation. produced by exudation of fibrin. Extensive adhesions are associated with multi-factors.The principal measures to prevent peritoneal adhesions are removing of exudate and foreign bodies during abdominal operations.Granulation composed mainly of spin die-shaped fibroblasts and capillaries forms by 72 hours, indicating that congelation caused by multi-factors can result in peritoneal adhesions at the initial stage.The effccient precautions against adhesions within three days need further research.There were no normal mesothelial cells, which have fibrinolytic activity, to be found in the study.The reason may be that plasminogen activating factor in the blood vessels beneath mesothelium is inhibited by ischemia,injury,infection,foreign bodies and hemorrhage. This is worth notice in the prevention and treatment.

Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

Objective To study the relationship between stabilization of patients with carotid artery atherosclerosis plaques and different traditional Chinese medicine (TCM)syndromes.Methods 60 patients with carotid artery atherosclerosis plaques were undertaken TCM syndrome differentiation and carotid Color Doppler ultrasonography tested.Results The cases of insufficiency of kidney essence and insufficiency of vital energy and blood had occupied a larger ration in stabilization carotid artery atherosclerosis plaques; while sputum and blood stasis locking collaterals syndrome and blood stasis due to Qi deficiency syndrome had statistical significance in unstabilization carotid artery atherosclerosis plaques.Conclusion Different TCM syndromes had different stabilization of carotid artery atherosclerosis.Sputum and blood stasis locking collaterals syndrome and blood stasis due to deficiency syndrome are unstable.These two syndromes are high risk syndromes of carotid artcry atherosclerosis plaques.

After many targeted drugs have entered into the treatment guideline or all stage clinical trial of non-small-cell lung cancer (NSCLC), many researchers undertake deep investigation on the role of molecular gene marker in targeted therapy of NSCLC. It is a good outset that mutation detection of epidermal growth factor receptor (EGFR) gene direct the selection of EFGR tyrosine kinase inhibitors (EGFR-TKI). To choose individualized therapy regimens according to molecular gene marker will be the important research direction, but also be the critical measure to improve the management level of NSCLC and prolong patient"s survival. Accompanied with deep investigation of targeted therapy, there will be more molecular gene markers to direct the formulation of individualized therapy regimens.

Objective To observe the clinical efficacy of acupuncture plus rehabilitation training in improving post-stroke dysphagia. Method A total of 60 patients with post-stroke dysphagia were randomized into a treatment group and a control group, 30 cases in each group. The two groups were intervened by basic treatment of internal medicine and swallowing rehabilitation training, and the treatment group was additionally given acupuncture treatment. The Standardized Swallowing Assessment (SSA), water swallowing test and Swallowing Quality of Life Questionnaire (SWAL-QOL) were evaluated in the two groups before and after the treatment. Result After the treatment, the SSA scores in the two groups were significantly decreased compared to those before the treatment (P<0.01), and the SWAL-QOL scores were significantly increased compared to those before the treatment (P<0.01); the SSA score in the treatment group was significantly lower than that in the control group (P<0.01), and the SWAL-QOL score was significantly higher than that in the control group (P<0.05). The water swallowing test classification after the treatment was significantly superior to that before the treatment in the treatment group (P<0.01), and there was a significant difference compared to that in the control group (P<0.05). Conclusion Acupuncture plus rehabilitation training can obviously improve the swallowing function of patients with post-stroke dysphagia, and the clinical efficacy was superior to single rehabilitation training.

OBJECTIVE To explore the correlative fators of nosocomial infections of preterm infants and decrease the infections rate of preterm infants.METHODS A retrospective analysis of nosocomial infection was made among preterm infants in our hospital from Jan 1998 to Dec 2005.RESULTS The incidence rate of nosocomial infections of preterm infants was 12.28%.The most infection site was respiratory tract.Small gestational age,low birth weight and birth asphyxia were the risk factors of nosocomial infections.CONCLUSIONS Intensive care of preterm infants and rational use of antibiotics are effective methods to decrease the rate of nosocomial infections.

The emergence of drug resistance mutations in human immunodeficiency virus (HIV) genome is the major reason for antiviral treatments failure.To investigate the drug-resistance viral quasispecies in HIV-infected individuals and to find out the new drug resistance mutations have significant effects on directing effective clinical treatment and developing novel antiviral drugs.HIV drug resistance tests mainly include genotypic drug susceptibility assay and phenotypic drug susceptibility assay.We reviewed in this article some novel development in the field.

Background and purpose:CD4+CD25+ regulatory T (Treg) cells are an important naturally accruing subpopulation of regulatory T cells. They not only prevent the spontaneous emergence of autoimmune diseases and contribute to the establishment of dominant tolerance on allogeneic transplantation, but also play an essential role in the generation, development and immunotherapy of many different kinds of malignant tumors. We studied the expression of CD4+CD25+ regulatory T cells(Tregs) in colorectal cancer patients and its significance. Methods:The tissues of tumors and counterpart normal tissue of 30 colorectal cancer patients were collected, the proportion of CD4+CD25+ Tregs as the percentage of the total CD4+ T cells were analyzed using flow cytometry and immunohistochemistry.Results:Compared with counterpart normal tissue(5.5?1.3)%, the percentage of CD4+CD25+ Tregs in tumor tissues was significantly higher (24.1?4.8)%(t=5.155,P=0.002). The proportion of CD4+CD25+ Tregs in the patients with lymph nodes metastasis was (27.9?3.6)% which was significantly higher than that in patients without lymph nodes metastasis[(20.3?1.3)% (t=3.489,P=0.025)].Conclusions:The proportion of CD4+CD25+ Tregs in tumor tissues of colorectal cancer patients was significantly increased, and they may play an essential role in the development of colorectal cancer.

Objective To establish a method for the determination of arsenic in urine by microwave digestion-oscillopolarographic method. Methods The urine samples were treated by microwave digestion. After being reduced to As3+,total arsenic in the samples was determined by oscillopolarographic method in the system of H2SO4-KBr-Se4+. The experiment conditions were optimized. Results The linear range of the method was 0-50 ?g/L(r=0.999 8),the limit of detection was 0.67 ?g/L,the relative standard deviation was 1.44%,and the rates of recovery were 95.0% -103.0%. Conclusion The method is simple,rapid,accurate and applicable to the determination of arsenic in human urine,which presents an advantage of low losing of arsenic in the treatment and determination.