OBJECTIVE:To investigate the variation degrees of the active components in the same batch No.GuanxinⅡdecoctions under the same decoction technics.METHODS:Crude drug of the same batch No.of the same formula was decocted,every decoction detail was tried to be kept under control,the contents of the active components in 5 batches of GuanxinⅡdecoctions were determined and the mean value of which was calculated as well.RESULTS:In terms of the contents of crude drugs,those of tanshinol,protocatechualdehyde,peoniflorin and ferulic acid were respectively(0.773?0.0 656)mg/g,(36.591?3.0 590)?g/g,(2.655?0.2 454)mg/g,(85.052?7.5 469)?g/g.CONCLUSION:Referenced by the criterion that the content variation coefficient among different batches of decoctions should be less than 10%,the contents of active components in each decoction were all up to the standards.Referenced by the criterion that there should be no statistical difference among different batches of decoctions,more influential factors on the active components of decoctions should be brought into consideration in the quality control.

OBJECTIVE:To study the presence of the chemical compositions of Guan XinⅡ(GXEH)oral decoction in human serum.METHODS:The analysis of finger prints of the medicated serum sample obtained from the volunteers treated with GXEH decoction was conducted with3different pretreatment procedures and2different HPLC methods,and which were compared with decoction.RESULTS:The numbers of the discrepant chromatographic peaks detected from the decoction and the medicated serum were respectively less than30and10,danshensu,protocatechuic aldehyde,ligustrazine,paeoniflorin were failed to be traced in the latter.CONCLUSION:The numbers of chemical compositions of the compound preparation that can be traced in serum were limited and the active components that entered into body may be relatively limited in numbers.After oral administration of compound preparation,a part of chemical compositions of which may not presented as its original form in serum.

OBJECTIVE: To investigate the concentration- time curve of ferulic acid(FA) in serum of healthy male volunteers after oral administration of Guanxin II (GXEH) and to study its pharmacokinetic parameters.METHODS: Serum FA level was determined by HPLC in healthy male volunteers after single oral administration of GXEH(4.5g?kg-1) decoction. RESULTS: No double - peak occurred in the concentration - time curve of FA.6 -fold interindividual variation was noted in maximum blood drug level.The pharmacokinetic parameters were stated as follows: Ka: (0.26?0.350) min-1; Ke: (0.013?0.004 17)min-1;t1/2Ke: (63.45?32.288)min;tmax:(24.117?14.631) min, AUC:(13 263.51?6 478.275)ng?min-1?mL-1; CL/F(s):(0.000 44?0.000 268)L?g-1?min-1.CONCLUSION:There is a significant individual FA level difference in healthy male volunteers after oral oral administration of GXEH decoction,and both the absorption and the elemination of FA are fast.

Objective To study the arterial stiffness in maintenance hemodialysis (MHD)patients and to explore the associated factors. Methods Ninety MHD patients and 36 healthy controls were enrolled in the study.Automatic pulse wave velocity (PWV) measuring system was applied to examine carotid-femoral pulse wave velocity(CFPWV)and carotid-radial PWV(CRPWV)as the parameters reflecting central elastic large arterial and peripheral muscular medium-sized arterial elasticity respectively.Blood pressure and biochemical parameters were detected.Backward muhiple linear regression analysis was used to assess the influencing factors of arterial stiffness.Results CFPWV and CRPWV in MHD patients were greater than those of healthy controls [(13.22±3.23)m/s vs(10.67±2.11)m/s and(9.58±1.87)m/s vs(8.81±1.09)m/s,all P＜0.05].Backward multiple regression analysis demonstrated that age and pulse pressure were positively correlated with CFPWV,while dialysis duration,calcium phosphorus product,parathyroid hormone,low density lipoprotein and mean arterial blood pressure were signiticantly correlated with CRPWV.Volume reduction after single dialysis session could not improve arterial stiffness ofMIlD patients.Conclusions Arterial stiffness of large artery and peripheral muscular medium-sized artery increases in MHD patients.Age and pulse pressure are major determinants of large artery stiffness.Peripheral muscular medium-sized arterial stillness is more easily influenced by dialysis-associated factors.

Objective To explore the effects of ingestion of purified drinking water from water treatment devices on health of rats. Methods Under the same feeding conditions, the Wistar rats were divided into different exposure groups exposed to different kinds of drinking water such as electrolytic ion water (alkaline water), reverse osmosis water, middle hollow fabric filtered water, copper-zinc ally (KDF) filtered water, municipal water and source water respectively. The changes of the indexes including organ coefficient, routine blood test, blood biochemistry and electrolyte were observed at the 3rd and the 6th month of the exposure. Results Higher levels of the count of white blood cells and neutrophil, and higher concentrations of seralbumin and total albumin in serum were observed in rats of tap water group compared with those in control group (P

Objective To investigate the influence of abnormal lipid metabolism on prognosis and quality of life in patients with severe COPD. Methods 134 patients with severe COPD in our hospital from September 2013 to January 2015 were selected as research objects, and their blood lipid levels were detected. The patients were divided into the normal group (n=61) and the low level group (n=73) according to the test results of lipid levels. 1-year mortality rate was statistically analyzed in both groups , and the WHOQOL-BREF health survey profile was applied to assess quality of life before and after treatment. Results 1-year survival rate in the normal group was 90.16%(55/61), which was higher than 73.97%(54/73) in the low level group (χ2=5.741, P=0.017). As compared with the normal group, the scores on physiological function, environment, social relationship, mental state, spirit / religious / belief, and total quality of lifewere markedly lower in the low level group after treatment (P < 0.05). The correlation analysis showed that plasma levels of TC, TG, and HDL-C were negatively correlated with 1-year mortality rate in patients with severe COPD (P < 0.05) but positively related with all dimensions in quality of life; plasma HDL-C level was positively related with 1-year mortality rate (P < 0.05), but negatively related with all dimensions in quality of life in patients with severe COPD (P < 0.05). Conclusions For patients with severe COPD, abnormal lipid metabolism is closely related with prognosis and quality of life. We should pay attention to nutritional support and control of abnormal lipid metabolism in those patients , leading to improvement of prognosis and quality of life.

Adult ; Biopsy ; Follow-Up Studies ; Humans ; Lithiasis ; diagnostic imaging ; pathology ; surgery ; Lung ; diagnostic imaging ; pathology ; surgery ; Lung Diseases ; diagnostic imaging ; pathology ; surgery ; Male ; Pulmonary Alveoli ; diagnostic imaging ; pathology ; surgery ; Tomography, X-Ray Computed

Objective To explore the thyroid function changes of episodes period and remissions period in children with primary nephrotic syndrome (NS).Methods The urinary protein quantitation and serum albumin,total cholesterol,thyroid hormone level [total triiodothyronine (TT3),total thyroxine (TT4),free triiodothyronine (FT3),free thyroxine (FT4) and thyroid stimulating hormone (TSH)] of episodes period and remissions period in 15 children with primary NS were retrospectively analyzed.Results There were statistical differences in urinary protein quantitation and serum albumin,total cholesterol in children with NS between episodes period and remissions period (P ＜ 0.05).The TT3,TT4,FT3,FT4 in episodes period were significantly lower than those in remissions period [(0.6 ± 0.3) μ g/L vs.(1.3 ± 0.3) μ g/L,(40.0 ± 23.0)μ g/L vs.(68.0 ± 15.0) μ g/L,(1.6 ± 0.3) pmol/L vs.(3.3 ± 0.3) pmol/L,(8.6 ± 2.5) pmol/L vs.(12.2 ± 2.0) pmol/L],TSH was significantly higher than that in remissions period [(5.6 ± 2.1) mU/L vs.(2.5 ± 1.1) mU/L],there were statistical differences (P ＜ 0.05).Conclusions Thyroid function of episodes period in children with primary NS is decreased temporarily,associated with heavy proteinuria leads to thyroid hormone missing from urine.Detecting thyroid function plays an important role in estimating the state and guiding the treatment of primary NS.

BACKGROUND: The use of bone graft materials can promote bone fusion and enhance the stability of the spine during the spinal fusion. OBJECTIVE: To investigate the effect of autologous bone marrow mesenchymal stem cel s with hydroxyapatite/ tricalcium phosphate in the spinal fusion. METHODS: A retrospective analysis of clinical data of 64 patients with spinal fusion was carried out, and these patients were divided into two groups (n=32 per group): control group undergoing autogenous iliac bone grafting and observation group undergoing autologous bone marrow mesenchymal stem cel s combined with hydroxyapatite/tricalcium phosphate. Al patients were fol owed up for 12 months, and their recovery conditions about low back pain, spinal fusion and vertebral reset were assessed. RESULTS AND CONCLUSION: The low-back outcome scale scores and excel ent rate, Lenke grading and Cobb angle had insignificant differences between the two groups after treatment (P > 0.05). No infection, inflammation and skin irritation occurred in the two groups. The coagulation function, renal function and inflammatory factor levels were at normal levels in al the patients, and there was no difference between the two groups (P > 0.05). These findings indicate that autologous bone marrow mesenchymal stem cel s combined with hydroxyapatite/tricalcium phosphate can achieve clinical outcomes equivalent to the autologous iliac bone grafting.

Actins ; metabolism ; Adult ; Antigens, Neoplasm ; metabolism ; Diagnosis, Differential ; Epithelioid Cells ; chemistry ; pathology ; Female ; Humans ; Hysterectomy ; Immunohistochemistry ; Melanoma-Specific Antigens ; Mesenchymoma ; metabolism ; pathology ; surgery ; Neoplasm Proteins ; metabolism ; Uterine Neoplasms ; metabolism ; pathology ; surgery