BACKGROUNDTo evaluate the efficacy of pulmonary artery infusion (PAI) via post-catheter connecting system in patients with lung cancer after lobectomy.

METHODSNinety patients were randomized into two groups after lobectomy and each group had 45 patients. The PAI group received chemotherapy via pulmonary artery, while the VI group received chemotherapy via peripheral vein.

RESULTSThe PAI group totally received 215 cycles of chemotherapy, 4.8 cycles in average. The VI group received 195 cycles, 4.3 cycles in average. The 1-, 3-, 5-year survival rates in PAI group were 88.9%, 77.8%, 47.4% respectively, and 82.2%, 53.3%, 20.5% in VI group. And there were significant differences in the 3- and 5-year survival rates between the two groups (P < 0.05). The 3-year local recurrence rate was 12.8% for radical lobectomy patients in PAI group, and 35.0% in VI group (P < 0.05). The 1-, 3-, 5-year metastatic rates after lobectomy were 17.8%, 20.0%, 26.3% respectively in PAI group, and 15.6%, 35.6%, 51.3% respectively in VI group. And there were significant differences in the 3- and 5-year metastatic rates between the two groups (P < 0.05).

CONCLUSIONSPulmonary artery infusion for lung cancer patients after lobectomy can reduce the post-operative recurrence and metastasis and improve the long-term survival rates.

Background and objectivehTe inhibitory ability of miR-424 on the proliferation of renal carcinoma cell and the migration and invasion of cancer cells has been widely explored and demonstrated. However, the effects of miR-424 on non-small cell lung cancer (NSCLC) have not been systematically examined. In this study, detected the growth and inva-sion effect of miR-424 in NSCLC A549 cell. hTe migration and molecular mechanism of this cell are also detected.Methods NSCLC A549 cell was transfected with miR-424 and its inhibitor. Atfer transfection, the proliferation ability of A549 cell was detectedby CCK8 assay. hTen, the migration ability in A549 cell was detected by migration assays. Furthermore, the expression level of MMP2 and MMP9 in A549 was detected by Western blot and immune lfuorescence. hTe 3'UTR of E2F6 was cloned into luciferase reporter vector and its enzymatic activitywas detected to verify whether miR-424 can target E2F6. hTe expres-sion level of E2F6 in a549 cell atfer transfecing with miR-424 was detected by Western blot.Results Atfer transfection of miR-424, the proliferation and migration abilities were remarkably decreased and the expression level of MMP-2 and MMP-9 were down-regulated in A549. Moreover, MiR-424 inhibited the enzymatic activity of luviferase reporter vector of E2F6. Spe-ciifcally, the expression level of E2F6 was down-regulated in A549.ConclusionmiR-424 can inhibit the proliferation and migration abilities of A549 by negatively regulating the expression of E2F6.