Objective Identification of viral replication in patients with HB without HBeAg is significant for estimating course and adopting appropriate therapy.This research aimed to clarify clinical signification of detecting Pre-S1Ag for identifing viral replication in patients with HB without HBeAg.Methods Sera samples were collected from 65 outpatients with HB confirmed by liver biopsy. HBV markers ,Pre-S1 antigen in sera were detected by ELISA;HBV-DNA by PCR;HBsAg, HBcAg and Pre-S1Ag in tissue by immunohistochemistry method.Results Intracellular HBsAg,Pre-S1Ag mainly expressing in the plasma account for 78.4% (51/65) ,69.2%(45/65) respectively, HBcAg partly in the plasma and the nucleus account for 67.6(44/65).Positive rate of expressing HBcAg,Pre-S1Ag are little less than HBsAg, but very near between Pre-S1Ag and HBcAg.There are no significant difference by ?~2 test (P=0.736). There are no significant difference between HBV-DNA (≥10~3copies/ml) and Pre-S1Ag(P=0.059),but there are significant difference HBV-DNA-positive are 18, in which 14 are Pre-S1Ag-positive.It demonstrates that there are viral replication in 18 of 30 HBeAg-negative patients and Pre-S1Ag corresponds considerably with HBV-DNA. Among 14 HBeAg-negative patients there are 4 cases with Pre-S1Ag without HBeAg because of Pre-C mutation.Conclusion Pre-S1 is a valuably serological marker for HBV replication without HBeAg.

Objective Study the relationship between the component of medium and spore formation of Bacillus sp WTFY. Methods Alter the carbon source、nitrogen source、inorganic concentration and kinds in turns, observe the sporulation circumstances of Bacillus sp WTFY.Results When glucose concentration was higher over 0.1%, spore did not emerge;Nitrogen source、phosphorus、magnesiumions concentration and kinds have some degree influence,but not significantly.Conclusion Carbon source concentration is a key factor that influence spore formation.

Objective To study the role of caspase 3 in ischemic brain damage of rats, and further understand the molecular mechanisms of ischemic cerebral vascular disease.Methods Rat models of the left middle cerebral artery (MCA) occlusion/reperfusion were made using a modification of the intraluminal sature method of Longa established by Belayev, infarct zones were confirmed by 2,3,5 triphenyltetrazolium chloride (TTC) staining, and caspase 3 expression on brain sections at the mRNA and active protein level was detected with in situ hybridization and immunohistochemistry technique, respectively.Results After 2 hours of left MCA ischemia followed by 24 hours of reperfusion, obvious infarct in the MCA dominate regions was confirmed by TTC staining; low levels of caspase 3 mRNA, and fewer of its active protein expression was found in normal brains, sham brains and contralateral brains of MCAO rats; both caspase 3 mRNA and activated protein expression in ipsilateral region were increased after 24 hours of recirculation, and even higher levels were detected at 48 hours of reperfusion.Conclusion Apoptotic mechanism might involve in delayed neuronal death after cerebral ischemia, and caspase 3 might play an important role in ischemic neuronal injury.

Objective To observe the changes of specific proteins in serum of acute stage patients with SARS and its clinical application. Methods The acute stage patients with SARS group included 42 cases who had clinical symptoms less than five days. In normal group: 30 healthy adults were as controls. Tests for specific proteins were immunoglobin G ( IgG ) , immunoglobin A ( IgA) , immunoglobin M ( IgM) , complement C3( C3),complement C4(C4),C-reaction protein (CRP), al acid glycoprotein( a1-AG) , a1 antitrypsin(a1-AT) ,transferrin(TRF), ceruloplasmin( CER) in sera by Ratenephelometer. Results The level of CRP, a1-AG, a1-AT in sera of patients with SARS were higher than that in control group ( P 0. 05). Conclusion It suggested that specific proteins in serum of acute stage patients with SARS change in a particular way. There were some help for the diagnosis of the phlogistic degree of acute stage patients with SARS by detecting CRP, a1-AG and TRF simultaneously.

Objective To explore the microsurgical treatment of the fourth ventricular tumors. Methods A total of 29 cases of the fourth ventricular tumors were given microsurgical treatment from January 2000 to June 2003 in this hospital. In 5 cases the tumor was located close to the cortex with the superior vermis involvement and the microsurgical operation was performed through the cerebellar vermis. The remaining 24 cases underwent microsurgical resection of tumor through the cerebellomedullary fissure approach. Results A total resection was achieved in 23 cases, and a subtotal resection in 6 cases. Postoperative complications included 2 cases of hemorrhage of the upper digestive tract, 1 case of mutism, 1 case of hematoma of the fourth ventricular, 1 case of epidural hematoma remote from surgical site, and 1 case of irregular breath. Follow-up surveys in 19 cases for 3~12 months (mean, 5.5 months) revealed no tumor recurrence. Conclusions Preoperative proper differentiating characters of tumors, clarifying the position of tumors' basal part, selecting appropriate surgical approach, and understanding microanatomy of the fourth ventricular are the key to successfully complete the procedure.

Objective To explore the nutrition condition improvement function of Jianpihuazhuoshengxue mixture to the long-term maintenance hemodialysis patient. Methods Before the treatment, to determine the nutrition condition of 43 cases dialysis patient that are divided to treatment group and control group. The two groups are all dialyze, the treatment group are added of Jianpihuazhuoshengxue mixture. To measure nutrition condition of the two groups again after one treatment course. Results and Conclusion Jianpihuazhuoshengxue mixture can remarkably raise the dialysis patient's nutritional level, especially the plasma albumin level. Possibly the mixture improvement the nutrition condition of dialysis patient mainly through raising the plasma albumin level, thus improve quality of life of the dialysis patient.

Objective:To explore the functional mechanism for therapeutic effects of angelica amylose in mice and rats with adjuvant-induced arthritis (AIA) and its effect of abirritation.Methods:Adjuvant arthritis was induced in either mice or rats.The effect of angelica amylose on rats’ arthritis and on mouse writhing response caused by acetic acid were observed.Tripterygium wilfordii was used as the positive drug for control.Results:Angelica amylose had obvious inhibition on mice writhing response and rats’ adjuvant-induced arthritis.Conclusion:Angelica amylose has effects of abirritation and anti-AIA.

Objective To investigate 1762T、1764A double position mutation in the Basic Core Promoter(BCP) of hepatitis B virus and reveal its relation to clinical symptoms and HBeAg phenotype. Methods microplate sandwich hybridization technique was used to detect BCP double position mutation. One common capture probe and one mutant specific detector probe as well as one wild type detector probe were synthesized and hybridized with amplified HBV DNA from the sera of hepatitis B patients. The results of hybridization were exhibited with ELISA. Results 147 hepatitis B patients who had been confirmed HBV DNA positive were screened. 51 patients were BCP double position mutation, 42 of which were BCP single position mutation and, 9 were mix mutation(both mutation and wild type were positive). BCP mutant was detected in 36 of 117 with chronic hepatitis and, 8 of which were mix mutants. Moreover, BCP mutant was detected in 7 of 30 with acute hepatitis in 25 of 78 with HBeAg positive were mutant and in 26 of 65 with HBeAg negative were mutant.Conclusions (1) The rate of BCP double position mutation in chronic hepatitis B patients is higher than that in acute hepatitis B patients. (2) BCP mutation may impair HBeAg expression. (3) PCR microplate sandwich hybridization ELISA is a sensitive and efficient method for detecting gene mutations.

0.05), while those of HBV DNA and ALT coincided poorly (P

Objective: To study caspase-3 activities and the neuroprotective effect of caspase-3 inhibitor Ac-DEVD-CMK in primary cultures of rat hippocampal neurons during hypoxia/reoxygenation (H/R). Methods: After pretreated with Ac-DEVD-CMK or the vector DMSO fo 1 h, primary cultures of rat hippocampal neurons were induced to hypoxia for 3 h, followed by 12 to 48 h of reoxygenation. The neuronal viability was estimated by MTT assay, and caspase-3 cellular activities were measured by a colorimetric method using Ac-DEVD-pNA as a substrate. Results: During H/R, the cultured rat hippocampal neuronal viability gradually decreased, and caspase-3 activities in the primary cultures of rat hippocampal neurons were significantly increased, and peaked at 24 h of reoxygenation. Caspase-3 activities were decreased in the neurons pretreated with Ac-DEVD-CMK in a dose dependent manner. Conclusion: Delayed neuronal death is detected in cultured hippocampal neurons during H/R, and apoptosis mediated by caspase-3 may play an important role in it, and caspase-3 inhibitor has a neuroprotective effect on them.