Objective To observe the effect of salt-partitioned moxibustion at Zhongwan (CV12) in treating belching due to chemotherapy.Method Subjects with chemotherapy-induced belching were randomized into an observation group and a control group, 30 in each group. The control group was intervened by intramuscular injection with Ritalin 20 mg, while the observation group was by salt-partitioned moxibustion at Zhongwan for 30 min in addition to the intramuscular injection.Result The total effective rate was 93.3% in the observation group versus 76.7% in the control group, and the difference was statistically significant (P＜0.05); the action latency in the observation group was (1.7±1.8)min in the observation group versus (15.6±4.2)min, and the difference was statistically significant (P＜0.01).Conclusion Salt-partitioned moxibustion at Zhongwan is significantly effective in treating chemotherapy-induced belching, and it’s easy-to-operate, efficient, and safe.

Objective: To investigate the clinical significance of the monocyte count/high-density lipoprotein cholesterol ratio(MHR)in evaluating imperfect ST-segment resolution in elderly patients with acute ST-elevation myocardial infarction(STEMI)after percutaneous coronary intervention(PCI). Methods: This was a retrospective cohort study.A total of 274 elderly patients with STEMI underwent PCI in our hospital from December 2015 to December 2018 were enrolled.Based on the extent of the ST-segment resolution of the postoperative electrocardiogram, patients were divided into an imperfect ST-segment resolution group(observation group, n=79)and a favorable ST-segment resolution group(control group, n=195). General clinical data were compared between the two groups, and logistic regression equation was used to analyze the association of MHR with ST-segment resolution.Receiver operating characteristic(ROC)curve was performed to assess the predictive value of MHR for imperfect ST-segment resolution. Results: Compared with patients in the control group, patients in the observation group were associated with a significantly higher proportion of anterior wall myocardial infarction and heart failure(≥Killip 2), A longer duration of chest pain to balloon expansion, higher levels of creatine kinase isoenzyme, N-terminal pro-brain natriuretic peptide, hypersensitive C-reactive protein, blood sugar, blood uric acid, fibrinogen, triglyceride and mononuclear cell count, and lower levels of high density lipoprotein cholesterol and lymphocyte count(all P<0.05). Meanwhile, there was a significant difference in MHR between the observation group and the control group [(0.75±0.22)vs.(0.48±0.19), t=9.831, P=0.001]. Multivariate Logistic regression analysis showed that MHR was an independent risk factor for imperfect ST-segment resolution(OR=1.950, 95%CI: 1.646-5.430, P=0.003)and ROC curve showed the threshold value of MHR at 0.67, the area under the curve at 0.867, the sensitivity at 79.72%, and the specificity at 79.61%. Conclusions MHR may be an independent risk factor and a good predictive index for imperfect ST-segment resolution in elderly patients with STEMI after PCI.

The aim of this study is to establish a multiplex polymerase chain raction (PCR) to identify of four kinds of laboratory animal pathogens: Pasteurella multocida, Bordetella bronchiseptica, Mycoplasma pneumoniae and Klebsiella pneumoniae.Methods Specific primers were designed based on GenBank data.The multiplex PCR system was established through optimization of multiple PCR and detection of its specificity and sensitivity.This technique was used to test artificially infected samples and tracheal secretions of experimental animals (rat, mouse, guinea pig, rabbit, hamster), and comparing the detection results by this method and traditional detection test.Results Target bands of Pasteurella multocida (356 bp), Bordetella bronchiseptica (237 bp), Mycoplasma pneumoniae (266 bp), and Klebsiella pneumoniae (142 bp) were obtained, with a detection sensitivity of Klebsiella pneumoniae of 10 pg, and that of Pasteurella multocida, Bordetella bronchiseptica and Mycoplasma pneumoniae of 1 pg by this newly developed multiplex PCR assay.No target bands were observed from the non-specific pathogens of artificially infected samples.The tracheal secretions taken from 45 experimental animals (mice and rabbits) were tested with this new PCR assay, among which 15 cases of Klebsiella pneumonia and 9 cases of Pasteurella multocida were detected as positive, while all the results of traditional method and serological test were negative.Conclusions A simple, rapid, specific and highly sensitive multiplex PCR system has been successfully established.It is valuable for detection of Pasteurella multocida, Bordetella bronchiseptica, Mycoplasma pneumoniae, and Klebsiella pneumoniae in laboratory animals.

BACKGROUND:The low oxygen environment after femoral fracture and cerebral trauma wil induce series of related cytokines expression, including hypoxia-inducible factor 1αand core binding factorα1, which play key roles in regulating bone healing. However, whether the accelerated bone healing is correlated with the expression of hypoxia-inducible factor 1αand core binding factorα1 is stil unknown.
OBJECTIVE:To construct rat models of brain injury, to compare the expression level of hypoxia-inducible factor 1αand core binding factorα1 in femoral fracture combined with cerebral trauma rats and simple femoral fracture rats, and to assess the influence of cerebral trauma on bone healing.
METHODS:Rats were randomly divided into blank group, simple femoral fracture group and femoral fracture combined with cerebral trauma group. At 1, 2, 3 and 5 weeks after modeling, rats were executed. Bone healing was evaluated using femoral fracture end X-ray score and hematoxylin and eosin staining at cal us tissues. Besides, the expression levels of hypoxia-inducible factor 1αand core binding factorα1 of three groups were determined with immunohistochemistry.
RESULTS AND CONCLUSION:Bone healing in the femoral fracture combined with cerebral trauma group was better than that of simple femoral fracture group. There was significant difference in the expression level of hypoxia-inducible factor 1αand core binding factorα1 between the simple femoral fracture group and femoral fracture combined with cerebral trauma group (P<0.05). At the same time, the level of simple femoral fracture group and femoral fracture combined with cerebral trauma group was significantly higher than that of blank group, and that in femoral fracture combined with cerebral trauma group was significantly higher than that of simple femoral fracture group (P<0.05). Results verified that the expression levels of hypoxia-inducible factor 1αand core binding factorα1 of rats with femoral fracture combined with cerebral trauma were significantly high, which may be the major reason why the bone healing was accelerated after fracture combined with brain injury.

In this study we synthesized a micro- and mesoporous material, SBA-16. And later on we functionalized it with octyltrimethoxysilane and octadecyltrimethoxysilane, respectively. The materials of SBA-16 and its functionalized form were characterized by nitrogen adsorption isotherms at 77K, small angle X-ray scattering (SAXS), Fourier-transform infrared (FT-IR), thermal gravimetric analysis (TGA), and adsorption isotherms of single component n-heptane, toluene and water vapour. The data of FT-IR and TGA demonstrated the successful chemical modification of surface and porous wall of SBA-16 with different hydrocarbon chains. The results of SAXS, nitrogen adsorption at 77K, and adsorption isotherms of probe molecules revealed that the functionalized SBA-16 materials possessed relatively less regularity, smaller BET surface area and pore volumes, and lower adsorption capacities for the probe molecules compared to the original SBA-16. However, the functionalized SBA-16 materials showed much less affinity to polar molecules such as water. This work provides useful fundamental information for future study of novel mesoporous silica materials as potential drug delivery carriers.
Adsorption ; Drug Carriers ; chemistry ; Porosity ; Silicon Dioxide ; chemistry ; Surface Properties

Subcutaneous panniculitis-like T-cell lymphoma (SPTL) is an extremely rare, indolent skin malignancy that can be difficult to distinguish from autoimmune disease-associated panniculitides. Here, we describe a 12-year-old boy who was diagnosed at age 7 years with dermatomyositis with classical manifestations, including poikiloderma, Gottron’s sign, and symmetric muscle weakness. Recently, the boy presented multiple subcutaneous nodules and fever. Histopathological examination and immunohistochemical staining revealed coexistence of SPTL. To our knowledge, this is the first case of dermatomyositis accompanied with SPTL. This case alert clinical physicians of the possibility of SPTL should be considered when a patient with dermatomyositis has new lesions presenting as nodules and unknown fever.

Objective To investigate the efficacy of micrornas(miR)-27B-3P combined with miR-215 in predicting the recurrence of early gastric cancer after endoscopic submucosal dissection(ESD).Methods A total of 122 patients with early gastric cancer admitted to the hospital from January 2021 to February 2022 were selected.All patients received ESD surgery and were followed up for 10～15 months,average(12.57±1.83)months.Patients with early gastric cancer were divided into recurrence group and non-recurrence group according to whether they had recurrence after ESD surgery.The relative expression levels of miR-27b-3p and miR-215 and clinical data of the two groups were compared to analyze the factors affecting the recurrence of early gastric cancer patients after ESD surgery,and analyze the relative expression levels of miR-27b-3p and miR-215 and the predictive value of their combination for the recurrence of early gastric cancer patients after ESD surgery.Results After 10～15 months,average(12.57±1.83)months of follow-up,3 cases of 122 patients were lost to follow-up,the follow-up rate was 97.54％,of which 12 cases relapsed,and the remaining 107 cases did not relapse.The miR-27b-3p in the relapsed group was lower than that in the non-relapsed group(P＜0.05),and the relative expression of miR-215 in the relapsed group was higher than that in the non-relapsed group(P＜0.05).The proportion of patients with lesion size ≥3 cm and infiltration depth of SM2-SM3 in recurrence group was higher than that in non-recurrence group(P＜0.05),and the proportion of patients with R0 resection in recurrence group was lower than that in non-recurrence group(P＜0.05).Logistic multivariate regression analysis showed that the lesion size was ≥3 cm and the depth of invasion was SM2-SM3,the relative expression level of miR-27b-3p,the relative expression level of miR-215 were the influential factors for recurrence of early gastric cancer patients after ESD operation(P＜0.05).The relative expression levels of miR-27b-3p and miR-215 and their combined prediction of the area under the curve(AUC)of ESD postoperative recurrence in early gastric cancer patients were 0.783,0.845 and 0.935,respectively.Conclusion miR-27b-3p and miR-215 can be used to predict the recurrence risk of early gastric cancer patients after ESD surgery,and the combination of Mir-27B-3P and Mir-215 has higher predictive value.

Self-advocacy can improve cancer survivors' symptom burden, quality of life, and well-being, which also plays a pivotal role in promoting cancer survivors' participation in medical decision-making and self-management. Based on research in China and abroad, this article introduces the concept of cancer survivors' self-advocacy and its role in cancer survivors, expounds the relevant factors that affect self-advocacy, and analyzes the application status and limitations of assessment tools and intervention methods. This article puts forward a prospect for the future research on self-advocacy of cancer survivors in China, in order to promote the participation in medical decision-making and the quality of self-management of cancer survivors in China from the new perspective of self-advocacy.

Ferroptosis is regarded as a novel and regulatable form of cell death, with its main characteristics being abnormal iron metabolism and the accumulation of lipid peroxides. Although there have been breakthroughs in the research of ferroptosis in tumors and degenerative diseases, its research in skin diseases is relatively scarce. Starting from the development of the concept of ferroptosis, this article expounds the basic characteristics and regulatory mechanisms of ferroptosis. In the field of skin diseases, we emphasize the importance of ferroptosis and discuss in detail the research and application of ferroptosis in various skin diseases, aiming to provide effective guidance for the treatment and clinical practice of various skin diseases, and offer new ideas for the future research direction of skin diseases.

Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.
Apoptosis ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs ; biosynthesis ; genetics ; RNA, Neoplasm ; biosynthesis ; genetics ; Stomach Neoplasms ; genetics ; metabolism ; pathology ; Up-Regulation