Objective To investigate the effect of lidocaine on the chemotherapy sensitivity of gastric cancer cells by regulating the Wnt/β-catenin axis.Methods Human gastric cancer cells SGC-7901 in logarithmic growth phase were inoculated into 96-well plates and treated with different concen-trations of lidocaine(0,10,50,100,150,200 μmol/L)for 24 h.The cell viability at different con-centrations was compared.The SGC-7901 cells in logarithmic growth phase were divided into control group,cisplatin group,low concentration lidocaine group(Lido-L group),medium concentration lido-caine group(Lido-M group),high concentration lidocaine group(Lido-H group),high concentration lidocaine+Wnt/β-catenin signal pathway activator SKL2001 group(Lido-H+SKL2001 group).The cell proliferation,invasion,and migration abilities of each group were compared by 5-acetylidene-2'de-oxyuracil nucleoside(EdU)cell proliferation detection,Transwell assay,and scratch healing experi-ment.The apoptosis of each group was detected by TUNEL assay.The expressions of apoptosis,epi-thelial-mesenchymal transition,and Wnt/β-catenin pathway-related proteins in each group were detec-ted.Results Compared with 0 μmol/L lidocaine,the cell viability of SGC-7901 cells treated with 50,100,150,and 200 μmol/L lidocaine was reduced(P＜0.05).Compared with the control group,the EdU positive rate,the number of cell invasion,scratch healing rate,and expressions of vimen-tin,N-cadherin,B-cell lymphoma-associated protein-2(Bcl-2),cyclin D1,scattered protein 2(DVL2),Wnt family member 3a(Wnt3a),β-catenin(β-catenin)were reduced in the cisplatin group,while the cell apoptosis rate was increased,the expressions of E-cadherin,B-cell lymphoma-2-associated X protein(Bax),Cleaved-caspase-3 were increased,and the differences were statisti-cally significant(P＜0.05).Compared with the cisplatin group,the EdU positive rate,the number of cell invasion,scratch healing rate,and expressions of vimentin,N-cadherin,Bcl-2,cyclin Dl,DVL2,Wnt3a,β-catenin were gradually reduced in the Lido-L group,Lido-M group,and Lido-H group,while the cell apoptosis rate,the expressions of E-cadherin,B-cell lymphoma-2-associated X protein(Bax),Cleaved-caspase-3 were increased(P＜0.05).Compared with the Lido-H group,the EdU positive rate,the number of cell invasion,scratch healing rate,and expressions of vimen-tin,N-cadherin,Bcl-2,cyclin D1,DVL2,Wnt3a,β-catenin were increased in the Lido-H+SKL2001 group,while the cell apoptosis rate and expressions of E-cadherin,Bax,Cleaved-caspase-3 were reduced,the differences were statistically significant(P＜0.05).Conclusion Lidocaine may enhance the chemotherapy sensitivity of gastric cancer cells by inhibiting the activation of the Wnt/β-catenin axis.

Objective To investigate the effect of lidocaine on the chemotherapy sensitivity of gastric cancer cells by regulating the Wnt/β-catenin axis.Methods Human gastric cancer cells SGC-7901 in logarithmic growth phase were inoculated into 96-well plates and treated with different concen-trations of lidocaine(0,10,50,100,150,200 μmol/L)for 24 h.The cell viability at different con-centrations was compared.The SGC-7901 cells in logarithmic growth phase were divided into control group,cisplatin group,low concentration lidocaine group(Lido-L group),medium concentration lido-caine group(Lido-M group),high concentration lidocaine group(Lido-H group),high concentration lidocaine+Wnt/β-catenin signal pathway activator SKL2001 group(Lido-H+SKL2001 group).The cell proliferation,invasion,and migration abilities of each group were compared by 5-acetylidene-2'de-oxyuracil nucleoside(EdU)cell proliferation detection,Transwell assay,and scratch healing experi-ment.The apoptosis of each group was detected by TUNEL assay.The expressions of apoptosis,epi-thelial-mesenchymal transition,and Wnt/β-catenin pathway-related proteins in each group were detec-ted.Results Compared with 0 μmol/L lidocaine,the cell viability of SGC-7901 cells treated with 50,100,150,and 200 μmol/L lidocaine was reduced(P＜0.05).Compared with the control group,the EdU positive rate,the number of cell invasion,scratch healing rate,and expressions of vimen-tin,N-cadherin,B-cell lymphoma-associated protein-2(Bcl-2),cyclin D1,scattered protein 2(DVL2),Wnt family member 3a(Wnt3a),β-catenin(β-catenin)were reduced in the cisplatin group,while the cell apoptosis rate was increased,the expressions of E-cadherin,B-cell lymphoma-2-associated X protein(Bax),Cleaved-caspase-3 were increased,and the differences were statisti-cally significant(P＜0.05).Compared with the cisplatin group,the EdU positive rate,the number of cell invasion,scratch healing rate,and expressions of vimentin,N-cadherin,Bcl-2,cyclin Dl,DVL2,Wnt3a,β-catenin were gradually reduced in the Lido-L group,Lido-M group,and Lido-H group,while the cell apoptosis rate,the expressions of E-cadherin,B-cell lymphoma-2-associated X protein(Bax),Cleaved-caspase-3 were increased(P＜0.05).Compared with the Lido-H group,the EdU positive rate,the number of cell invasion,scratch healing rate,and expressions of vimen-tin,N-cadherin,Bcl-2,cyclin D1,DVL2,Wnt3a,β-catenin were increased in the Lido-H+SKL2001 group,while the cell apoptosis rate and expressions of E-cadherin,Bax,Cleaved-caspase-3 were reduced,the differences were statistically significant(P＜0.05).Conclusion Lidocaine may enhance the chemotherapy sensitivity of gastric cancer cells by inhibiting the activation of the Wnt/β-catenin axis.

Objective: To understand the relations between high risk sexual behavior and HIV infection among MSM in ways of finding male partners in Ningbo. Methods: A cross-sectional study was conducted in Ningbo between April and November in 2018. Data related to socio-demographics, ways of finding male partners, adoption of gay apps and sexual behaviors were collected by snowball method. Blood samples were drawn for HIV antibody testing. Classified data was evaluated by chi-square test. Related factors on HIV infection were analyzed by multivariate logistic regression. Results: A total of 735 participants were included in this study. Ways of finding male partners would through gay apps (60.8%, 447/735), QQ/Wechat (32.3%, 237/735) and gay-places (6.9%, 51/735). Related information on high risk sexual behavior and HIV infection among gay apps users were found as: 16.8%(75) had sexual behavior once per week in the past 6 months, 41.8% (187/447) had multiple sexual partners, 12.1% (54/447) had unprotected anal intercourse in the last time, 52.3% (234/447) having had unprotected anal intercourse in the past 6 months. The overall HIV prevalence rate was 12.1%(54/447). Among the HIV cases who got infected within the two years, 68.6%(24/35) of them had used gay apps for less than two years. Results from the, multivariate logistic regression analysis showed that gay apps users were more susceptible to infected HIV than those who used the QQ/Wechat (OR=3.03, 95%CI: 1.30-7.07). Conclusions Gay apps was popularly known among the Ningbo MSM, and was associated with the high risk sexual behaviors and HIV infection. HIV control and prevention programs should be strengthened in the MSM population who used the gay apps. Related surveillance and intervention programs for MSM, who use the gay apps, need to be further reinforced.