The practices of military hospital medical equipment operation training were introduced, and the problems were pointed out in training content, organization and support. Some suggestions were put forward from the aspects of field medical equipment development, training plan, post-based training and etc so as to enhance the supportability of field medical equipment.

Objective By studying the acute brucellosis patients' blood culture and the bacteria strains,we tried to confirm the bacteria strains,and to further explore the symptoms and the results of the treatment.Methods Totally 122 acute brucellosis patients were selected,who were in hospital in Heilongjiang Provincial Nongken General Hospital from 2012 to 2013.The patients aged 6-73 years old,whose average age was 36.5(including 83 males and 39 females).All of the subjects were detected in the aspects of blood culture and brucellosis serology,all of whom were treated with aminoglycoside,4-quinolones and cephalosporins.Results All the subjects were Brucella melitensis biovar,of which 104 were Brucella melitensis biovar 3,18 were Brucella melitensis biovar 1.All of them had positive results in rose bengal plate agglutination test (RBPT).In serum tube agglutination test (SAT),the valence of Brucella melitensis biovar 1 and 3 were uniformly distributed,Brucella melitensis biovar 1 was distributed mainly in 1∶800 valence zone,Brucella melitensis biovar 3 was distributed mainly in 1∶400 valence zone.The distribution trends in Coombs test and complement fixation test (CFT) were similar.In Coombs they were distributed mainly in 1∶800 valence zone,and in CFT were distributed mainly in 1∶40 zone.All of the subjects had typical symptoms of brucellosis,and had various kinds of complications (spondylitis,toxipathic hepatitis,et al).Totally 112 of them were recovered,and 10 were improved.Conclusion The human brucellosis in Heilongjiang Province is caused by Brucella melitensis biovar 1 and 3,with severe symptoms and various kinds of complications,earlier diagnose and treatment will have a better effect.

Objective Co-stimulation cells is a kind of natual killer (NK)-like T cells, which can kill tumor cells.Previous studies show that lupeol , an natural plant extracts , can change the growth of NK cells ,γδT cells and their effects on tumor cells .This study aimed to investigate the effects of lupeol on human co-stimulation cells and colonic cancer cell lines SW 480 . Methods The peripheral blood mononuclear cells ( PBMC) from healthy volunteers were induced in vitro by different cytokines and transferred into Co-simulation cells.After SW480 and Co-stimulation cells were incubated with different concentrations of lupeol for different durations , methyl thiazolyl tetrazolium (MTT) was used to detect the effects of lupeol on co-stimulation cells and colonic cancer cell lines SW480. Lactate dehydrogenase was used to determine the cell-killing activity of Co-simulation cells to colonic cancer lines SW 480 . Results The concentration of lupeol in 0.1-200.0 mg/L promoted the growth of Co-stimulation cells and inhibited the colonic cancer cell lines SW480.When the concentration of lupeol is at 12.5 mg/L, the cell-killing activity of Co-simulation cells to colonic cancer lines SW 480 was enhanced significantly compared with the controls (76%vs 40%, P<0.05). Conclusion Lupeol could promote the prolifera-tion of Co-stimulation cells, inhibit the growth of cancer lines SW480, and strengthen the cytotoxicity of Co-stimulation cells against co-lonic cell lines SW480.

Objective To study the influence situation of amoxicillin and clavulanate potassium for the postoperative infection and inflammatory indexes of patients with emergency intestinal obstruction operation by relaxation suture .Methods 80 patients with emergency intestinal obstruction operation by relaxation suture in department of general gurgery of our hospital from April 2014 to April 2016 were randomly divided into control group and observation group with 40 cases in each group, the control group were treated with routine treatment, the observation group were treated with amoxicillin and clavulanate potassium on the treatment of control group, then the postoperative infection rates, inflammatory related indexes before and after the treatment of two groups were compared.Results The postoperative infection rate of observation group was 5.00%,and it was obviously lower than the 17.50% of control group, the difference was significant(P<0.05); the serum inflammatory related indexes of two groups before the treatment were compared,the differences were not significant ;the serum anti-inflammatory indexes interleukin-4(IL-4), IL-10, IL-13 and pro-inflammatory indexes tumor necrosis factor-α(TNF-α), IL-6 at 1st, 2nd and 5th day after the treatment were all lower than those of control group,the pro-inflammatory index IL-2 were higher than those of control group, the differences were all significant(P<0.05).Conclusion The amoxicillin and clavulanate potassium can reduce the postoperative infection of patients with emergency intestinal obstruction operation by relaxation suture , and its control role for the related inflammatory indexes is better, so its application value is higher and is worthy of clinical promotion.

Development of small molecule drugs is important for targeted and personalized therapy for cancers.However,it is staggering to take new compounds from the research bench to clinical trial pipelines and eventually to the clinical practice,due to the time,effort and costs.Most drugs need five years of development after discovery in the laboratory before they are ready to be tested for efficacy and safety.Compared to development of new drugs from scratch, increasing research efforts have been made to turn existing drugs to new uses to treat cancers, which may bypass years of costly work.Metformin used in cancer care is a good example of giving old drugs a new life.The activity of metformin on anti-cancer has recently drawn significant attention.Epidemiological and experimental studies have shown that metformin can cut down the incidence of cancer in diabetic patients and reduce metabolism-related cancers.Meanwhile, recent study has found metformin can induce autophagy of esophageal cancer cells by inhibiting inflammatory signaling pathway.In recent years, increasing studies have shown that metformin plays a major role in suppressing cancers via multiple mechanisms.In this mini-review,we summarize the updates of the research on metformin in cancer prevention and treatment.

Objective:To study the effect of GABA and A receptor agonist THIP on proliferation and apoptosis of cytokine-induced killer (CIK) cells.Methods:CIK cells were cultured by routine method,and then treated with different concentrations of GABA and THIP.The proliferation of CIK cells were investigated by MTT assay.The apoptosis,cell cycle and immunophenotype were investigated by flow cytometry.Results:GABA could inhibit the proliferation of CIK cells in a dose-dependent manner and affect the distribution of cell cycle of CIK cells(P

Objective:To observe the effects of auto-dendritic cell vaccine on T cells of patients with condyloma acuminatum(CA) related human papilloma virus(HPV) and its clinical outcome.Methods:73 patients with condyloma acuminata(CA) in the present study were positive of HPV-DNA and have been unsuccessfully treated with freezing,laser,or electric therapy combined with interferon anti-virus treatment for half a year.The peripheral blood samples were harvested from patients and the monouclear cells were extracted to cultivate DCs in presence of cytokines.Auto DCs were sensitized with corresponding HPV virus antigen of a patient to prepare the auto-dendritic cell vaccine.Then the vaccine were cultivated with auto T-cells and the proliferation of T cells were examined.Meanwhile,the auto-DC vaccine(1-4)?107 was also injected into the patients' inguinal lymph glands and its therapeutic effectiveness was observed.Results:The prepared DC-vaccine significantly promoted the proliferation of auto T cells compared with control group(P

Objective To analyze the relationship of the lateral angulation with the incision level after anterior approach operation for thoracolumbar fractures by evaluating the Cobb' s angle at different approach levels.Methods A retrospective study was done on 154 patients with thoracolumbar fractures treated consecutively with anterior operation from May 2004 to January 2008.The preoperative,postoperative and follow-up angle of coronal Cobb and the postoperative angle between screws and endplates on the anteroposterior radiograph were measured.According to the relationship between the injury vertebrae and the incision level,the patients were divided into two groups,ie,incision level ≥2 vertebrae group and incision level ≤ 1 vertebra group.Results All patients were followed up for 6-47 months(mean 37 months),which showed significant postoperative lateral angulation(P ＜ 0.01)especially at follow-up (P ＞ 0.05).The coronal Cobb' s angle showed insignificant difference before operation between two groups but it was increased after operation and during the follow-up(P ＜0.01).The screws A or B were more parallel to the endplate in two groups(P ＜ 0.01),while the parallel of the screws C or D to the endplate showed no statistical difference(P ＞ 0.05).Conclusions Compared with the operation with incision level ≥2 vertebrae,the operation with incision level ≤ 1 vertebra can more easily induce postoperative lateral angulation and exert a significant impact on implantation orientation of the vertebral screws A and B,which may indirectly lead to postoperative lateral angulation.

ObjectiveTo observe the effect of betulinic acid(BetA) on the growth of human cytokine induced killer(CIK) cells and the killing activity of CIK cells on the gastric cancer cells in vitro before and after induced by betulinic acid,explore its mechanism.MethodsPeripheral blood mononuclear cell (PBMC) were separated form the healthy and were induced with various of cytokine to become CIK cells in vitro.CIK cells were collected on the tenth day and were induced with betulinic acid in different concentrations,followed by 48 h,the colorimetric methyl thiazolyl tetrazolium(MTT) method assay the proliferation rate of human CIK cells.Flow cytometry (FCM) was used to detect the expression changes of perforin,granzyme B and CD107a of human CIK cells before and after betulinic acid-induced.Lactate dehydrogenase (LDH) release assay was used to measure the influence on cytotoxic activity of CIK cells induced by betulinic acid against gastric cancer cell line SGC-7901 in vitro.Western blot assay was used to measure the extracellular signal-regulated kinase1/2 (ERK1/2),and adapter proteins SH2-domain containing leukocyte protein of 76KD(SLP-76) and linker for activative of T cells(LAT) expression changes of human CIK cells before and after drug-induced.ResultsBetulinic acid can promote CIK cells growth when the concentration were in 0.08-10 μg/ml,the expression of perforin,granzyme B and CD107a of CIK cells were significantly higher than control group(P＜0.05) when the concentration of betulinic acid were in 0.3 μg/ml.In the meanwhile,the cytotoxic activity of CIK cells in vitro against gastric cancer cell line SGC-7901 were also remarkably higher than the control group (P＜0.05).The expression of SLP-76,LAT and ERK1/2 were significantly increased to a certain extent than the control group( P＜0.05 ),when CIK cells were treated with betulinic acid.ConclusionThese results suggest that betulinic acid can promote CIK cells growth in some concentrations and increase the cytotoxic activity of CIK cells against gastric cancer cell line SGC-7901,its mechanism may related with two factors,on the one hand,enhancing the activity of SLP-76,LAT and ERK1/2,on the other hand,increasing the expression of perforin,granzyme B and CD107a on the surface of CIK cells.

Objective To investigate the in vitro effects of quercitrin on the proliferation and the cytotoxicity of human γδT cells.Methods Peripheral blood mononuclear cells (PBMCs) were isolated from healthy subjects and cultured with isopentenyl pyrophosphate and IL -2 to induce human γδT cells.The hu-manγδT cells were cultured with quercitrin at various concentrations for 48 hours.CCK-8 kits were used to analyze the in vitro proliferation and cytotoxic activities of γδT cells.Flow cytometry was performed to meas-ure the expression of granzyme B and perforin in γδT cells.The expression of p-ERK, p-Akt and Bcl-2 at protein level were detected by Western blot .Results The percentage of human γδT cells in PBMCs was in-creased from (2.96±1.83)%to (88.94±2.36)%after 10 days of culture.The quercitrin at concentrations of 10 to 80 μg/ml could promote the growth of γδT cells and up-regulate the expression of granzyme B , per-forin, p-ERK, p-Akt and Bcl-2 in a dose dependent manner .The cytolytic activities of γδT cells against co-lonic carcinoma cells ( HCT116 ) were enhanced by quercitrin .Conclusion Quercitrin could promote the proliferation of γδT cells and enhance the expression of granzyme B and perforin at certain concentrations in vitro.ERK1/2 and Akt signal transduction systems might be involved in the process .