Objective Twelve cases with small advanced cole-rectal cancer less than 10 mm in diameter were examined. Methods The endoscopic findings and clinicopathologic data of 12 cases with advanced colorectal cancer less than 10 mm in diameter were compared with those larger than 10 mm in diameter. Results Approximated estimation of the macroscopic types were Ⅱ c in 5 cases, Ⅱ a in 3 cases, and I s in 4 cases respectively. As for the distribution of these lesions, there were 5 in sigmoid colon, 4 in transverse colon, 1 in cecum, and 2 in descending colon. No lesions were detected in rectum. Histologic grade at the deepest invasive portion was shown well, moderately, and poorly differentiated in 2, 8 and 1 case respectively and another case with mucinous adenocarcinomas. Lymphnode metastasis was present in 4 of 12 lesions (33% ). This incidence is rather high, as regards to the small size of each lesion. Endoscopi-cally, all lesions accompanied with converging folds and 7 lesions examined by magnifying colonoscope showed V N pit pattern. Conclusion These results indicated that the advanced colorectal cancers less than 10mm in diameter showing similar characteristics with those of superficial depressed type cancer with likely is the precurser of advanced cancer. The findings of converging folds and pit pattern are considered to be the useful indicators for estimating the depth 'of invasion.

[Objective]To investigate the pathological change of articular cartilage after impacting injury.[Method]Fifty-six New Zealand adult rabbits were divided randomly into low-energy and high-energy groups,with 28 rabbits in each group.One side of hind limb was designed as experimental side and the opposite one as control side.Four rabbits were killed at 4 days,1,2,4,8,16 weeks after impacting injury.Histological sections of the specimens were observed and stained by HE and Safranin O.the values of gray scale were obtained by imaging analysis.[Result]In low-energy group,the Mankin's score and the value of gray scale of Safranin O in experiment side was not significantly different from the control side.In high-energy group,the Mankin's score and the value of gray scale of Safranin O in experiment side increased gradually,and was significantly different from the control side.[Conclusion]In the low-energy group,the cartilage returned to the normal level at 4 weeks.In the high-energy group,the cartilage failed to be restored.The degeneration of articular cartilage was very obvious after 4 weeks.

Objective:To observe the effects of auto-dendritic cell vaccine on T cells of patients with condyloma acuminatum(CA) related human papilloma virus(HPV) and its clinical outcome.Methods:73 patients with condyloma acuminata(CA) in the present study were positive of HPV-DNA and have been unsuccessfully treated with freezing,laser,or electric therapy combined with interferon anti-virus treatment for half a year.The peripheral blood samples were harvested from patients and the monouclear cells were extracted to cultivate DCs in presence of cytokines.Auto DCs were sensitized with corresponding HPV virus antigen of a patient to prepare the auto-dendritic cell vaccine.Then the vaccine were cultivated with auto T-cells and the proliferation of T cells were examined.Meanwhile,the auto-DC vaccine(1-4)?107 was also injected into the patients' inguinal lymph glands and its therapeutic effectiveness was observed.Results:The prepared DC-vaccine significantly promoted the proliferation of auto T cells compared with control group(P

Objective:To study the effect of GABA and A receptor agonist THIP on proliferation and apoptosis of cytokine-induced killer (CIK) cells.Methods:CIK cells were cultured by routine method,and then treated with different concentrations of GABA and THIP.The proliferation of CIK cells were investigated by MTT assay.The apoptosis,cell cycle and immunophenotype were investigated by flow cytometry.Results:GABA could inhibit the proliferation of CIK cells in a dose-dependent manner and affect the distribution of cell cycle of CIK cells(P

Objective To investigate the bioactivities of controlled release bFGF microspheres (Ms) and their effects on the cultured osteoblasts. Methods The secondary cultured osteoblasts were divided into four groups according to the different ingredients being added to the DMEM culture medium, ie, control group,bFGF group, bFGF-PLGA-Ms group and bFGF-PELA-Ms group. The proliferation of the cultured osteoblasts was measured with cell counting method, MTT method and flow cytometry. The content of bone BGP secreted by osteoblast was also measured with RIA method. Results The in vitro cellular study showed no significant difference in the cell number and cell viability of four groups one day after plate culture.The cell number and cell viability in the bFGF-PLGA -Ms group were more than those in other three groups four and six days after plate culture. The cell number and cell viabilitythose in the bFGF group were more than those in the bFGF-PELA-Ms group six and eight days after plate culture with insignificant difference. The flow cytometrical examination showed that the G 2/M+S percentage in the bFGF group reached the highest two days after plate culture and the G 2/M+S percentage in the bFGF-PLGA-Ms group went the highest four and eight days after plate culture. Among four groups, the content of BGP in the bFGF-PLGA-Ms group was the highest and the bFGF-PELA-Ms group the next. Conclusions The effect of bFGF-PELA-Ms is not satisfactory,as indicates that the manufacturing method needs improving. However,the bFGF-PLGA-Ms can promote the proliferation and differentiation of the osteoblasts through a long period of controlled release of bFGF.

OBJECTIVE:To establish UV-spectrophotometry method in the determination of metformin hydrochloride in compound metformin hydrochloride tablets.METHODS:The determination was carried out with water as the solvent under the detection wavelength of233nm.RESULTS:There was a good linear relationship between metformin hydrochloride and its absorbability(r=0.9999)within the concentration range of1～10?g/ml.The average recovery was99.3%with RSD at0.24%(n=9).CONCLUSION:The method is accurate,fast,convenient,and which can be used to determine the contents of metformin hydrochloride in compound metformin hydrochloride tablets directly without isolation.

Objective To investigate the efficacy and safety of tranexamic acid on bleeding in rheumatoid arthritis (RA) patients undergoing total hip arthroplasty (THA). Methods A retrospective study was performed in 197 RA patients (Steinbrock?er III-IV) following primary unilateral THA from June 2012 to June 2014. The patients were divided to three groups based on the regimen of tranexamic acid:68 patients received a single intravenous dosage of 15 mg/kg tranexamic acid 20 min prior to opera?tion (single dose group);74 patients received an intravenous dosage of 15 mg/kg preoperatively and a second dosage of 10 mg/kg 3 hours postoperatively (repeated dose group);the other 55 patients didn't receive tranexamic acid (control group). The primary out?comes were total blood loss, transfusion rate, the incidence of deep vein thrombosis (DVT) and pulmonary embolism (PE). The sec?ondary outcomes were postoperative drainage, hemoglobin (Hb) drop on third day postoperatively and other wound related compli?cations. Results There was less total blood loss (816.80 ± 245.09 ml vs 975.15 ± 216.33 ml vs 1 295.68 ± 263.85 ml), drainage (221.60 ± 70.05 ml vs 337.20 ± 113.10 ml vs 479.74 ± 120.66 ml), transfusion requirement (5.41%vs 10.29%vs 25.45%) and Hb drop (2.71±0.74 g/dl vs 3.18±0.62 g/dl vs 3.83±0.70 g/dl) in experimental group when compared with control group. And the effect was better in repeated dose group, with less total blood loss (816.80 ± 245.09 ml), less transfusion requirement (5.41%) and less postoperative drainage (221.60±70.05 ml). No episode of DVT or PE occurred in either group. There were 8 wound complications in single dose group, 6 in repeated group, and 8 in control group, and there were no statistically difference. Conclusion Intrave?nous administration of tranexamic acid was effective and safe on decreasing blood loss and transfusion requirement in RA patients following THA. Compared with a single dosage of tranexamic acid preoperatively, a second dosage of tranexamic acid 3 hours post?operatively was recommended.

Objective By studying the acute brucellosis patients' blood culture and the bacteria strains,we tried to confirm the bacteria strains,and to further explore the symptoms and the results of the treatment.Methods Totally 122 acute brucellosis patients were selected,who were in hospital in Heilongjiang Provincial Nongken General Hospital from 2012 to 2013.The patients aged 6-73 years old,whose average age was 36.5(including 83 males and 39 females).All of the subjects were detected in the aspects of blood culture and brucellosis serology,all of whom were treated with aminoglycoside,4-quinolones and cephalosporins.Results All the subjects were Brucella melitensis biovar,of which 104 were Brucella melitensis biovar 3,18 were Brucella melitensis biovar 1.All of them had positive results in rose bengal plate agglutination test (RBPT).In serum tube agglutination test (SAT),the valence of Brucella melitensis biovar 1 and 3 were uniformly distributed,Brucella melitensis biovar 1 was distributed mainly in 1∶800 valence zone,Brucella melitensis biovar 3 was distributed mainly in 1∶400 valence zone.The distribution trends in Coombs test and complement fixation test (CFT) were similar.In Coombs they were distributed mainly in 1∶800 valence zone,and in CFT were distributed mainly in 1∶40 zone.All of the subjects had typical symptoms of brucellosis,and had various kinds of complications (spondylitis,toxipathic hepatitis,et al).Totally 112 of them were recovered,and 10 were improved.Conclusion The human brucellosis in Heilongjiang Province is caused by Brucella melitensis biovar 1 and 3,with severe symptoms and various kinds of complications,earlier diagnose and treatment will have a better effect.

Objective To investigate the association between the nodular gastritis and the eradication of Helicobacter pylori .Methods To the patients with nodular gastritis discovered by gastroendoscopy during 2001～2002, detection of Helicobacter pylori and eradication of it if positive, were performed then followed up after 6 months by endoscopies.Results From 4939 cases of endoscopy thirteen cases of female nodular gastritis were diagnosed, accounted to 0 26% of the total cases examined. The age of patients ranged from 14～49 years, with mean age 29 years.The main symptom is epigastralgia. All cases were found affected with H.pylori . After H.pylori eradication, there is disappearance of the nodular appearance of the gastric antrum under endoscopies and disappearance of the gastric submucosal lymphoid follicles under histological examination.Conclusion Nodular gastritis is the very important manifestation having a high positive predictive value in H.pylori infection.

Objective To investigate the relationship between brucellosis arthritis of the hip and femoral head necrosis.Methods In August 2007 to August 2013,16 diagnosed and hospitalized patients with brucellosis complicated with femoral head necrosis in Heilongjiang Land Reclamation Bureau General Hospital were chosen as the objects of observation,and the patients' hospitalization information was analyzed retrospectively.Patients' medical records were investigated and analyzed.Observations included general condition of the patient,clinical symptoms and signs,imaging,pathology,treatment and curative effect.Results All the 16 patients had definite clinical manifestations of hip arthritis and femoral head necrosis including hip pain,limited mobility,positive 4 test,limp or bedridden,and sick limb muscle atrophy.Imaging examination:X-ray computer tomography (CT) showed cystic change and deformation collapse of femoral head.Magnetic resonance imaging(MRI) showed low signal on weighted T1 images,high signal on weighted T2 and soft tissue granulation hyperplasia could be seen in the hip joint.Pathological examination:most femoral bone tissue necrosis,dissolved,bone cells disappeared,a few bone cells present,and some fibrous tissue hyperplasia.Patients were treated with antibiotics according to the treatment principles of brucellosis,and the effect was obvious.Conclusions There is a causal relationship between brucellosis arthritis of the hip and femoral head necrosis.Therefore,positive and effective treatment of brucellosis arthritis of the hip in the early stages is crucial to prevent the occurrence of femoral head necrosis.