Objective To evaluate the role of serum CA125 level in diagnosing the epithelial ovarian tumor.Methods Serum CA125 level of seventy-two patients who were operated for epithelial ovarian tumor between Apr 2005 and Jun 2007 in PUMC hospital were recruited and analyzed retrospectively.Using microparticle enzyme immunoassay(MEIA) to determine the serum CA125 level,and evaluate the serum CA125 level in diagnosing epithelial ovarian cancer.Results The CA125 was higher than 35?/ml in 35/39 ovarian serous cystadenocarcinoma cases with 89.7% positive rate,CA125 was higher than 35?/ml in 9/12 ovarian borderline serous cystadenoma with 75% positive rate,and CA125 was higher than 35?/ml in 4/21 ovarian serous cystadenoma with 19% positive rate.Conclusion The serum CA125 level before operation can predict benign epithelial tumor or epithelial ovarian cancer.

Objective To observe the occurring time of heparin-induced thrombocytopenia,resuming time and effects of treatment during continuous hemofiltration.Methods To select 9 cases of patients with continuous veno-venous hemofiltration(CVVH)hospitalized in Luohe centre hospital,70 cases of patients with hemoperfusion and 20 hemodiaglysis cases of patients with acute and chronic renal failure during 2005,1 to 2006,1,blood samples were collected from the patients,platelet was tested before and after the treatment,the bleeding symptoms and effects of treatment were observed.Results There was 1 case during the 27th hour of CVVH,the lowest platelet was 10?10～9/L,resumed 48h hours after stoping heparin;there was 1 case during the first hour of hemoperfusion,the lowest platelet was 67?10～9/L,resumed 60h hours after stoping heparin.Conclusions It is essential to observe the changes of disease condition closely,to test platelet and whenever necessary to give cortex hormone,hemostatics and interrupted using platelet transfusion.

Objective To explore the relationship between single nucleotide polymorphisms (rs12953-717) of SMAD7 and susceptibility of non-small cell lung cancer (NSCLC) in Chinese Han population.Methods A single nucleotide polymorphisms (rs12953717) from SMAD7 was detected via Sequenom system in 528 NSCLC cases and 762 healthy controls.Data was statistically analyzed by unconditional Logistic regression method.Results rs12953717 had significant differences between non-small cell lung cancer patients and the controls.Compared with CC/TT (CC combined with TT) genotype,the adjusted odds ratio for the CT genotype was 4.107 (95％ CI:3.206 ～ 5.260,P =0.000 1).Smokers had a 2.004 odds ratio (95 ％ CI =1.583 ～ 2.537,P =0.000 1) of NSCLC compared with the controls.There was a 10.074-fold increased risk of NSCLC among the subjects with CT genotype and smokers.Conclusion The polymorphism of rs12953717 may have relation with risk of NSCLC.Heterozygote (CT) is a susceptibility genotype of NSCLC.Smoking is one of the risk factors of NSCLC.Smoking and CT genotype have synergistic effects on NSCLC susceptibility.

OBJECTIVE To analyze the high incision infection rate in abdominal operation in order to take effective preventive measures.METHODS Totally 2302 cases of abdominal operation in general surgery were investigated retrospectively from Jan 2005 to May 2007.The diagnosis standard was based on the Diagnosis Standard of Hospital Infection(Draft) published by Ministry of Health of the People′s Republic of China in Jan 2001.RESULTS From them 113 cases suffered incision infection.The infection rate was 4.85%,accounted for 81.54% of all surgical operation incision infections.The pathogenic bacteria in the samples taken during the operation were the same as those in the secretion of postoperative incision in terms of species and source.CONCLUSIONS The key points of prevention of incision infection are sterile operation,flushing of abdominal cavity and enhanced detection of environment hygiene in operating room.The infection rate could be lowered through observation and nursing care of postoperative incision and proper use of antibiotics.

OBJECTIVE To strengthen the hospital infection management in community health service centers,in order to reduce the onset of hospital infection.METHODS According to Hospital infection administration regnltions and Shenzhen medical service hospital infection control quality evaluation management demands we had investigated and evaluated the hospital infection management in 9 community health service centers.RESULTS The monitoring,surveillance and management of hospital infection in community health service centers as a quality evaluation index of hospital medical service still had many problems,it should further enhance the quality in the field of administration management,sterilization and disinfection,surveillance and sevage treatment.CONCLUSIONS The hospital infection management in community health service centers is a part of whole hospital medical service,but their quality at present is weak.To strengthen the hospital infection management is important to guarantee the medical service quality and medical care safety.

OBJECTIVE To comprehend antimicrobial susceptibility distribution of the ?-lactamases products from Feb 2002 to Feb 2003 in our hospital.METHODS The Microscan-USA was used to identify pathogens and drug sensitivity tests,the results of examination were judged according to NCCLS 1999 standard.(RESULTS) Among the isolated 67 strains of ESBLs-producing bacteria(35.64%),31 strains of(Klebsiella) pneumoniae(36.05%),29 strains of Escherichia coli(43.28%), 6 strains of Enterobacter cloacae((22.22)%)and 1 of K.oxytoca(12.5%) were detected.The multi-resistance of ESBLs~+ bacteria was very usual,besides to the ?-lactam antibiotics.The pattern of being resistant to both of tobramycin and ciprofloxacin was relatively common,and amikacin was relatively less,even the complex antibiotics containing ?-lactamases inhibitor would lose their effect.(CONCLUSIONS)The ESBLs bacteria exist in our area at a high level and also be the main antimicrobial-resistant ones and have got the resistance to many kinds of antibiotics.To control the(ESBLs)~+(bacteria) infection,expanding and supervision management of antibiotics is a urged task and important for us.

Objective To study the relationship between quality of life and social support and ex-plore effective ways to improve quality of life in patients with cardiomyopathy. Methods In-hospital pa- tients (50 cases) with eardiomyopathy were randomly selected and inquired by applying general quality life inventory(GQOLI-74) and social support rating seale(SSRS),compared with 45 normal people as the con- trol group. Results Compared with the control group, quality of llfe (P < 0.05) and social support (P <0.01 ) in patients with eardiomyopathy decreased significantly.Social support was positively related with quality of life (r =0.614, P < 0.01 ). Conclusions Quality of life is closly related with social support in patients with cardiomyopathy. Improving quality of life by social support in patients with cardiomyopathy should be emphasized and make effective use of in the courses of nursing.

Objective To study the clinical characteristics of epidemic Japanese encephalitis in 4 adult patients and to improve the level of diagnosis.Methods The clinical characteristics, laboratory results and MRI features of 4 adult patients with epidemic Japanese encephalitis hospitalized in our hospital between 2007 and 2008 was retrospectively reviewed and analyzed.Results All patients were sporadic.Two were female and others were male with the age from 34 to 68 years old and onset in summer and autumn.One patient had diarrhea at onset while others had fever and headache at onset.All patients had high fever and psychiatric symptoms.Two patients had respiratory failure.Obvious elevation of white cells in cerebrospinal fluid was seen in all patients and Japanese viral antibody of IgM was all positive whereas the image of MRI was abnormal only in one patient.Two patients had neurological sequelae.Conclusions The clinical manifestations of epidemic Japanese encephalitis in adults are severe and untypical and the respiratory failure is likely to occur.

Objective:To investigate the effects of deep hypothermia on MAC,cardiac anesthetic index and myocardial stability of valotile anesthetics. Method: Forty New Zealand white rabbits were randomly divided into 4 groups:halothane, enflurane,isoflurane and sevoflurane. MAC of valotile agents were determined with the test of clamping tail at normal(38 C?0.5 C )and deep hypothermic(23 C?0.5 C )temperatures. The end-tidal valotile agent concentration at which ventricular arrhythmia appeared by increasing valotile agent concentration and stimulating the heart with extrathoracic electric shock(25V 50Hz)were recorded. Result:MAC of halothane, enflurane,isoflurane and sevoflurane were decreased by average percentage of 5.1,3.6,4.4 and 4.3 per degree from 38 C to 23 C respectively. Cardiac anesthetic indexes during deep hypothermia were 4.4,3.18.6.25 and 4.6. There was significantly higher incidence of ventricular fibrilation in halothane and enflurane groups (100％) than that in isoflurane and sevoflurane groups(40％)under 8 MAC anesthetics. Conclusion:Isoflurane is the most suitable valotile agent for deep hypothermic anesthesia, while enflurane is the least.

Objective To investigate the impacts of the left ventricular size for infants with total anomalous pulmonary venous connection(TAPVC) on the early results of anatomical correction.Methods From Jan 2010 to Jun 2013,103 cases of TAPVC children under 1 year of age received biventricular correction in our hospital,including 65 males and 38 females with the mean body weight of(5.3 ± 1.3) kg.Taking left ventricular end-diastolic volume index(LVEDVI) of 20 ml/m2 as a boundary,all the children were divided into two groups:Small LV group and the Near normal LVgroup.Various factors including age,body weight,pathological type,pulmonary venous obstruction and restricted atrial septal defect were compared between the two groups.The Z value were introduced to demonstrate the small extent of the left atrium and left ventricle of TAPVC patients in comparison with the normal children.TAPVC correction surgery were performed with conventional median sternotomy,moderate hypothermic cardiopulmonary bypass and combined malformations were treated simultaneously.Results 45 patients were classified to Small LV group and 58 patients were classified toNear normal LV group.71.1％ of all Small LV patients was diagnosed as the obstruction type of TAPVC,the ratio was significantly higher than that of theNear normal LV group.The Z value of left ventricular end-diastolic diameter in theSmall LV group was significantly lower than that of the Near normal LV group.The mean CPB and aortic clamping time of all patients were (96.6 ± 34.4) min and (58.0 ±21.1) min respectively.There were 4 early postoperative death and the overall mortality was 3.9％.No patient was dead of low cardiac output.The duration of postoperative mechanical ventilation,ICU stay and vasoactive drugs application in Small LV group was significantly longer than that of Near normal LV group.Conclusion TheSmall LV,which should be viewed as relative dysplasia of left ventricle ,is more common in obstructive type of TAPVC.As long as the sizes of mitral valve and aortic valve were not significantly reduced,anatomic correction can be implemented and need not to concern the reducing degree of left ventricle.Nevertheless,the prevention and treatment of low cardiac output in the operation and early postoperative period were still key points for small LV patients to achieve good surgical results.