1.Prior Immunization with Fusobacterium Nucleatum Interferes with Opsonophagocytosis Function of Sera against Porphyromonas Gingivalis.
Jeom Il CHOI ; Melinda A BORRELLO ; Christopher W CUTLER ; Maurice ZAUDERER
The Journal of the Korean Academy of Periodontology 2000;30(1):105-110
No abstract available.
Fusobacterium nucleatum*
;
Fusobacterium*
;
Immunization*
;
Porphyromonas gingivalis*
;
Porphyromonas*
2.Fusobacterium nucleatum modulates serum binding to Porphyromonas gingivalis biofilm.
Jeomil CHOI ; Soo Jin KIM ; Sung Jo KIM
The Journal of the Korean Academy of Periodontology 2001;31(4):661-668
No abstract available.
Biofilms*
;
Fusobacterium nucleatum*
;
Fusobacterium*
;
Porphyromonas gingivalis*
;
Porphyromonas*
4.Isolation of Fusobacterium nucleatum from subgingival plaque in Korean.
Hyun Seon JANG ; Seo Hoon KIM ; Hwa Sook KIM ; Joong Ki KOOK ; Mi Kwang KIM ; So Young YOO ; Byung Ock KIM
The Journal of the Korean Academy of Periodontology 2003;33(2):149-158
The purpose of this study was to isolate and characterize the Fusobacterium nucleatum (F. nucleatum) from subgingival plaque in Korean periodontitis patients. The subgingival plaque samples of periodontitis patient were collected with sterilized paper point. The paper point was put into reduced transfer medium and then immediately transferred to laboratory. The subgingival samples were diluted by 10,000 folds and plated on F. nucleatum-selective media agar plate. The plates were incubated at 37degrees C in an anaerobic chamber for 3 days. The violet-colored colonies were selected and subjected to further verification whether those are F. nucleatum or not. For further confirmation, 16S rRNA genes (rDNA) were cloned from each of bacterial clones and determined sequence of 16S rDNA. In this study, we found 17 distinct clinical isolates of F. nucleatum from subgingival plaque. The clinical isolates will be a useful in various studies in periodontology.
Agar
;
Clone Cells
;
DNA, Ribosomal
;
Fusobacterium nucleatum*
;
Fusobacterium*
;
Genes, rRNA
;
Humans
;
Periodontitis
5.Necrotizing Fasciitis on the Right Side of the Neck with Internal Jugular Vein Thrombophlebitis and Septic Emboli: A Case of Lemierre’s-Like Syndrome
Dang Nguyen ; Yazmin Yaacob ; Hamzaini Hamid ; Sobri Muda
Malaysian Journal of Medical Sciences 2013;20(5):70-78
Lemierre’s syndrome (LS) is a rare life-threatening infective condition typically starting with an oropharyngeal infection causing thrombophlebitis and metastatic abscesses. The most common aetiology of LS is Fusobacterium necrophorum; however, it can also occur after infection with other organisms. LS mainly affects young healthy adults. The initial infection site can be in the head and neck or in the abdomen. The morbidity rate of this disease is high despite aggressive treatments. In this article, we report a 63-year-old male patient with uncontrolled diabetes mellitus, presenting with Klebsiella pneumoniae infection-induced necrotizing fasciitis on the right side of the neck, leading to LS.
Fasciitis, Necrotizing
;
Lemierre Syndrome
;
Fusobacterium necrophorum
;
Klebsiella pneumoniae
6.Prior Exposure of Mice to Fusobacterium Nucleatum Modulates Host Response to Porphyromonas Gingivalis.
Han Yong SON ; Sung Jo KIM ; Jeom Il CHOI
The Journal of the Korean Academy of Periodontology 2000;30(3):675-685
Multiple periodontal pathogens sequentially colonize the subgingival niche during the conversion from gingivitis to destructive periodontal disease. An animal model of sequential immunization with key periodontal pathogens has been developed to determine whether T and B lymppocyte effector functions are skewed and fail to protect the host from pathogenic challenge. The present study was performed to evaluate immunomodulatory effect of exposure to Fusobacterium nucleatum(F. nucleatum) prior to Porphyromonas gingivalis(P.gingivalis). Group 1(control) mice were immunized with phosphate-buffered saline, Group 2 were immunized with F. nucleatum prior to P. gingivalis, while Group 3 were immunized P. gingivalis alone. All the T cell clones derived from Group 2 demonstrated type 2 helper T cell clone(Th2 subsets), while those from Group 3 mice demonstrated Th1 subsets. Exposure of mice to F. nucleatum prior to P. gingivalis interfered with opsonophagocytosis function of sera against P. gingivalis. In adoptive T cell transfer experiments, in vivo protective capacity type 2 helper T cell clones(Th2) from Group 2 was significantly lower than type 1 helper T cell clones(Th1) from Group 3 against the lethal dose infection of P. gingivalis. Western blot analysis indicated the different pattern of recognition of P. gingivalis fimbrial proteins between sera from Group 2 and Group 3. In conclusion, these study suggest that colonization of the subgingival niche by F. nucleatum prior to the periodontal pathogen, P. gingivalis, modulates the host immune responses to P. gingivalis at humoral, cellular and molecular levels.
Animals
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Blotting, Western
;
Clone Cells
;
Colon
;
Fusobacterium nucleatum*
;
Fusobacterium*
;
Gingivitis
;
Immunization
;
Mice*
;
Models, Animal
;
Periodontal Diseases
;
Porphyromonas gingivalis*
;
Porphyromonas*
7.An in vitro model of Fusobacterium nucleatum and Porphyromonas gingivalis in single- and dual-species biofilms
Lívia Jacovassi TAVARES ; Marlise Inêz KLEIN ; Beatriz Helena Dias PANARIELLO ; Erica DORIGATTI DE AVILA ; Ana Cláudia PAVARINA
Journal of Periodontal & Implant Science 2018;48(1):12-21
PURPOSE: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. METHODS: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. RESULTS: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. CONCLUSIONS: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.
Bacterial Adhesion
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Biofilms
;
Biomass
;
Fusobacterium nucleatum
;
Fusobacterium
;
Gentian Violet
;
In Vitro Techniques
;
Microscopy, Electron, Scanning
;
Polystyrenes
;
Porphyromonas gingivalis
;
Porphyromonas
8.Study of the inflammatory activating process in the early stage of Fusobacterium nucleatum infected PDLSCs.
Yushang WANG ; Lihua WANG ; Tianyong SUN ; Song SHEN ; Zixuan LI ; Xiaomei MA ; Xiufeng GU ; Xiumei ZHANG ; Ai PENG ; Xin XU ; Qiang FENG
International Journal of Oral Science 2023;15(1):8-8
Fusobacterium nucleatum (F. nucleatum) is an early pathogenic colonizer in periodontitis, but the host response to infection with this pathogen remains unclear. In this study, we built an F. nucleatum infectious model with human periodontal ligament stem cells (PDLSCs) and showed that F. nucleatum could inhibit proliferation, and facilitate apoptosis, ferroptosis, and inflammatory cytokine production in a dose-dependent manner. The F. nucleatum adhesin FadA acted as a proinflammatory virulence factor and increased the expression of interleukin(IL)-1β, IL-6 and IL-8. Further study showed that FadA could bind with PEBP1 to activate the Raf1-MAPK and IKK-NF-κB signaling pathways. Time-course RNA-sequencing analyses showed the cascade of gene activation process in PDLSCs with increasing durations of F. nucleatum infection. NFκB1 and NFκB2 upregulated after 3 h of F. nucleatum-infection, and the inflammatory-related genes in the NF-κB signaling pathway were serially elevated with time. Using computational drug repositioning analysis, we predicted and validated that two potential drugs (piperlongumine and fisetin) could attenuate the negative effects of F. nucleatum-infection. Collectively, this study unveils the potential pathogenic mechanisms of F. nucleatum and the host inflammatory response at the early stage of F. nucleatum infection.
Humans
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Fusobacterium nucleatum/metabolism*
;
NF-kappa B/metabolism*
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Periodontal Ligament/metabolism*
;
Signal Transduction
;
Fusobacterium Infections/pathology*
;
Stem Cells/metabolism*
9.Lemierre syndrome with thrombosis of sigmoid sinus following dental extraction: a case report.
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2013;39(2):85-89
Lemierre syndrome is caused by an infection in the oropharyngeal region with subsequent thrombophlebitis in the internal jugular vein. The thrombus from the thrombophlebitis can invade other vital organs, such as liver, lungs, or joints, resulting in secondary infection, which further exacerbates the fatal prognosis of this syndrome. Lemierre syndrome, also called postanginal sepsis or necrobacillosis, was first reported by Dr. Lemierre in 1936. In his report, Lemierre mentioned that out of 20 patients who suffered from this syndrome, only two survived. He also stated that all of the 20 patients complained of infections in the palatine tonsils and developed sepsis and thrombophlebitis in the internal jugular vein. Once called a "forgotten disease," this syndrome showed a very high mortality rate until usage of antibiotics became prevalent. In this case report, the authors present a 71-year-old female patient who suffered from Lemierre syndrome with thrombosis extended to the right sigmoid sinus.
Anti-Bacterial Agents
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Coinfection
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Colon, Sigmoid
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Female
;
Fusobacterium Infections
;
Fusobacterium necrophorum
;
Humans
;
Joints
;
Jugular Veins
;
Lemierre Syndrome
;
Liver
;
Lung
;
Palatine Tonsil
;
Prognosis
;
Sepsis
;
Thrombophlebitis
;
Thrombosis
10.Effect of Weissella cibaria on Fusobacterium nucleatum-induced Interleukin-6 and Interleukin-8 Production in KB Cells.
Mi Sun KANG ; Hoi Soon LIM ; Seon Mi KIM ; Hyun Chul LEE ; Jong Suk OH
Journal of Bacteriology and Virology 2011;41(1):9-18
Oral microorganisms, including pathogens together with commensals, interact with oral epithelial cells, which can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. Fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. Our previous data suggest that Weissella cibaria, an oral commensal, inhibits the proliferation of periodontopathic bacteria including F. nucleatum. The aim of this study was to examine the effects of W. cibaria on the inflammatory mediators, interleukin (IL)-6 and IL-8, in KB cells stimulated by F. nucleatum. In a reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, live F. nucleatum alone induced high levels of gene expression and protein release of IL-6 and IL-8, whereas W. cibaria alone did not induce IL-6 and IL-8 responses in KB cells. W. cibaria dose-dependently inhibited the increases of the IL-6 and IL-8 gene expression as well as IL-6 protein level in KB cells which was induced by F. nucleatum. Bacterial viability and its coaggregation with F. nucleatum are not essential in the inhibitory effect of W. cibaria. Visible effects of W. cibaria on the attachment and invasion of KB cells by F. nucleatum were observed. In conclusion, W. cibaria may exert immunomodulatory effects on the IL-6 and IL-8 responses to F. nucleatum-activated KB cells.
Bacteria
;
Enzyme-Linked Immunosorbent Assay
;
Epithelial Cells
;
Fusobacterium
;
Fusobacterium nucleatum
;
Gene Expression
;
Humans
;
Interleukin-6
;
Interleukin-8
;
Interleukins
;
KB Cells
;
Microbial Viability
;
Periodontal Diseases
;
Weissella