Periostin, a extracellular matrix protein structurally similar to fasciclin proteins, is expressed specifically in various tumor tissues and correlates with tumor progression and prognosis. Periostin prevents the apoptosis of tumor cells, promotes tumor growth and can induce angiogenic signaling cascades through αvβ3-FAK and Flk-1/KDR. It also contributes to epithelial-mesenchymal-transformation promoting tumor invasion and metastasis. Periostin maybe a potential therapeutic target of cancer treatment.

Objective To clone, locate and differentially analyze the transcription factor Relish gene from Anopheles stephensi, and to examine its signals-modulating action on prophenoloxidase cascade and melanization of Plasmodium yoelii oocysts. Methods Relish cDNA of total mosquitoes was amplified by RT-PCR with degenerated primers. Target PCR product was purified,cloned,sequenced and identified. Special Relish gene was amplified with specific primers from hemocytes or midgut,respectively. Semi-quantitative analysis was made under different feeding conditions. Relish message ribonucleic acid was identified with hybridization in situ. Results One cDNA segment of Relish similar to An.gambiae was acquired from An. stephensi. The same Relish gene was also manifested in the hemocytes and midgut. Marked up-regulation expression of Relish was observed at 6,12,24 or 48 h of Plasmodium yoelii infection and at 12 and 24 h after sucking nitroquine-acetate sucrose solution,that was before inducible oocyst melanization. Relish was also expressed in the hemocytes and midguts by ISH. Conclusion Transcription factor Relish of An. stephensi might play a role in signal modulation of Plasmodium yoelii infection and oocyst melanization.

Objective: To compare the effects of three fluoride-containing agents on the remineralization of deciduous teeth in vitro. Methods: 36 deciduous teeth were randomly divided into four groups with 9 in each. The teeth were fenestrated on the labial middle surface and the rest parts of the teeth were covered with red nail polish.Every exposed area was etched,rinsed and puffed.Then the exposed areas were treated with 100 g/L(NH 4) 2MoO 2F 4(group ①) , 380 g/L Ag(NH 3) 2F (group ②) and APF-LaCl 3(group ③) for 3 minutes respectively, those in group④ were treated with deionized H 2O as the controls.Then,the teeth were placed into a bottle containing 5 ml remineralization solutions. 4 days later, the concentration of Ca 2+ in the remineralization solutions was measured. Results: The Ca 2+ concentrations (mmol/L) in the remineralization solution of group ①,②,③ and ④ were 0.338 9?0.116 5,0.570 0?0.145 5,0.517 8?0.084 8 and 0.120 4?0.046 8 respectively (P0.05:②vs③). Conclusion: The results indicate that all of the three fluoride-containing agents can promote the remineralization of deciduous teeth in vitro, and that both APF-LaCl 3 and 380 g/L Ag(NH 3) 2F are more effective than 100 g/L(NH 4) 2, MoO 2 F 4.

Objective: To develop a PCR method for detecting S treptococcus sobrinus (S.s) in conventional culture. Method: A pair of specific primers were designed from the dexA gene of S.s , the genome DNA of 12 strains of Mutans Streptococci (8 serotypes from a ～h) and 23 species of the bacteria which were commonly found in oral cavity wer e tested by PCR amplification, the PCR products were identified by electrophore sis. Result: Only S.s of serotype d and g could produ ce 277 bp DNA fragments, and the PCR method could detect less than 1 000 copies of S.s. Conclusion: PCR method is specific and sen sitive in the detection of S.s.

BACKGROUND: Both proximal femoral nail and hemiarthroplasty have been applied in the treatment of unstable intertrochanteric fractures in osteoporotic patients. However, which has better curative efficacy is still under discussion.OBJECTIVE: To investigate the clinical effectiveness of proximal femoral nail versus hemiarthroplasty in the treatment of unstable intertrochanteric fracture in osteoporotic patients.METHODS: Sixty-seven patients diagnosised with unstable intertrochanteric fractures and osteoporosis were selected,and were randomly divided into two groups, followed by treated with hemiarthroplasty (group 1, n=35)) and proximal femoral nail (group 2, n=32), respectively. The operation time, intraoperative blood loss, length of incision, postoperative time in bed, hospitalization time and complications were recorded and compared between two groups. Harris hip scores were used to evaluate the preoperative and postoperative hip function.RESULTS AND CONCLUSION: (1) In the group 1, the operation time, intraoperative blood loss and length of incision were more than those in the group 1, and the postoperative time in bed was less than that in the group 2 (P < 0.05).However, the healing and hospitalization time did not differ significantly between two groups (P > 0.05). (2) No significant difference was observed in the excellent and good rate in Harris hip scores between two groups at the last follow-up (P >0.05). The postoperative pain scores in the group 1 were significantly superior to those in the group 2 (P < 0.05). (3)There were 1 case of delayed union and 1 case of lateral hip appearing with pain in the group 2. At the last follow-up, all patients healed completely, and no prosthesis dislocation, loosening or infection was found. (4) To condude,hemiarthroplasty provides early functional recovery and fewer complications, but with much surgical trauma. Proximal femoral nail shows similar treatment outcomes with hemiarthroplasty; therefore, it is a kind of alternative treatment strategy for unstable intertrochanteric fractures.

The protective function of five traditional herb medicines, namely anisodamine,escin,salvia miltiorrhizae radix,astragalus root and ecdysterone, in cultured HUVECs challenged by LPS. ATP,ADP,AMP and energy charge were measured in cultured HUVECs challenged by LPS after treatment with five herb medicines. The results showed that energy charge of HUVECs decreased after LPS challenge,and increased significiantly more afer the addition of escin,radix astragali and ecdysterone than other herbs.It suggested that escin, astragalus and ecdysterone can protect HUVECs from injury induced by LPS.

Objective To investigate the prevalence of bladder hyperactivity in Huzhou city,Zhejiang prov-ince.Methods 1 872 patients aged over 50 years old male residents were selected as research subjects in Huzhou. To investigate the prevalence of bladder hyperactivity in the elderly male population in the local area.The correlation between age,IPSS score,body mass index,diabetes and the bladder was investigated.Results In 1 872 cases,the data were complete and in accordance with the requirements of this study were 1 863 cases.1 863 cases,505 patients with bladder hyperactivity,bladder disease prevalence was 27.1%.Bladder hyperactivity patients with IPSS score (17.9 ±8.1 )points,prostate volume (36.6 ±18.5 )mL,QOL score (3.6 ±1.1 )points,residual urine volume (26.9 ±3.5 )mL in the bladder,were higher than those with non bladder hyperactivity,IPSS score (3.2 ± 16.8)points,prostate volume (28.1 ±1.3)mL,QOL score (2.3 ±1.8)points,the differences were statistically sig-nificant (t =7.277,5.910,19.814,2.406,P <0.01 -0.05).Qmax(12.6 ±6.3)mL/s in the patients with bladder hyperactivity was significantly lower than Qmax(17.1 ±7.4)mL/s of non bladder hyperactivity,and the difference was statistically significant (t =3.577,P <0.01).With the increasing age of male population,the prevalence of blad-der diseases was increased.With the increase of IPSS score,the incidence rate of bladder excessive activity increased. Diabetes,higher body mass index,the incidence of bladder excessive activity was higher.Conclusion Men with blad-der hyperactivity disorder has higher prevalence in Huzhou city,Zhejiang province.Age,lower urinary tract symptoms, diabetes,obesity are the risk factors in the incidence of bladder disease.

Students are often apt to make mistakes in operative dentistry and endodontics experiments because of the complex experiment procedures and various instruments.Practice shows that it can effectively prevent and correct many mistakes by reasonably using mistake-demonstration method in experiment teaching of operative dentistry and endodontics.

OBJECTIVE:To probe into the drug utilization data in the second half year of 2007 in our hospital.METHODS:By using ABC analytic method,the consumption amount,consumption sum and the varieties of drugs used in the second half year of 2007 in our hospital were analyzed statistically.RESULTS:Type A,B and C drugs accounted for 16.34%,18.76% and 65.03%,respectively,in terms of the consumption amount.CONCLUSION:The drug utilization structure in our hospital is rational on the whole,but the use of antibiotics is somewhat irrational and thus need to be standardized further.

Objective To explore primarily the differentially expressed proteins in the hemolymph from adult female Anopheles stephensi ( An stephensi ) infected with Plasmodium yoelii ( P yoelii ) after being fed with sucrose solution containing nitroquine or not at different time points Methods Hemolymph of 2 groups of adult female An stephensi was collected with the expulsion method from the first day to the fifth day after the feeding Hemolymph samples were examined with SDS PAGE The protein gels were visualized by either Coomassie brilliant blue or silver staining, scanned and automatically analyzed by the BioRad1000 gel image analysis system for differential proteins bands Results On the second day of feeding with nitroquine, a few oocysts were partially melanized Furthermore, during the period from the fifth day to the ninth day, the number of mosquitoes with malanized oocysts and the number of melanized oocysts gradually increased The number of hemolymph protein binds in the treatment group was markedly more than that in the control Many different bands, mainly located at the molecular weight of (20～40)?10 3 and (60～80)?10 3, were visualized in the 2 groups The number of protein bands stained by the silver staining was more than that by the Coomassie brilliant blue staining Conclusion There are differentially expressed proteins in the hemolymph in An stephensi infected with P yoelii after being fed with sucrose solution containing nitroquine These differential proteins may be the melanization engaging proteins