1.Immuno-evaluation and immunotherapy, new strategy of clinical therapy for chronic hepatitis B
Chinese Journal of Laboratory Medicine 2009;32(7):730-734
The immunopathogenosis of chronic hepatitis B is complex. The host immune response plays an important role in determining the turnover of chronic hepatitis B, which is impacted by HBV, host immune system and liver microenvironment. In clinical practice, interferin-α (IFN-α) which has both anti-virus and immuno-regulatary effect wins the advantage over nucleoside analogue. During treatment with INF-α, quantitative HBsAg can predict therapy early which can make physicians modulate individual therapy strategy and achieve better curative effect. To better understand the anti-HBV immune responses to chronic hepatitis B, scientists hope to explore more effective and potential immunotherapeutic strategies against this disease.
2.Study on correlation between brain natriuretic peptide and anthracyclin-induced cardiac toxicity in patients with lymphoma
Journal of Leukemia & Lymphoma 2013;22(3):169-171
anthracycline-induced cardiotoxicity in patients with lymphoma.Methods Thirty-two adult patients with nonHodgkin' s lymphoma who received chemotherapy including anthracyclin were studied.After anthracyclin reached the cumulative doses of 200 and 400 mg/m-2,the changes in plasma BNP and echocardiography indices were investigated.Results After the cumulative anthracyclin reached doses of 200 and 400 mg/m2,serum BNP were respectively (292±7) ng/ml and (387±4) ng/ml,and were significantly increased when compared to the untreated (134±6) ng/ml (P < 0.05).The parameter of diastolic functions ratio of peak early to peak late low velocity (E/A ratio)were 1.14±0.37 and 0.90±0.06,both showing significant decreases compared to the control (1.33±0.27) (P < 0.05).In contrast,systolic function parameters left ventricular ejection fraction (EF) and fractional shortening (FS) remained unchanged (P > 0.05).After the cumulative anthracyclin reached 200 and 400 mg/m2,significant negative correlations were observed between the plasma BNP and the E/A ratio (r =-0.689,P=0.042; r=-0.557,P =0.006),but no associations between EF and FS were found (P > 0.05).Conclusion Plasma BNP concentration appears to be a sensitive parameter for the early assessment of anthracycline-induced cardiotoxicity.
3.The Internal Control Role of Ribosomal Protein S7 in the Defense of Anopheles dirus Against Plasmodium Infection
Wenyue XU ; Fusheng HUANG ; Jianhua DUAN
Chinese Journal of Parasitology and Parasitic Diseases 1997;0(05):-
Objective To investigate the role of ribosomal protein S7(rpS7) in the defense of Anopheles dirus against infection. Methods rpS7 was amplified from Anopheles dirus hemocytes with degenerated primers designed according to the conservative region of S7, rpS7 was then cloned using T/A cloning kit and the inserted fragment was sequenced. The difference of the transcript abundance of rpS7 from Anopheles dirus hemocyte among non-blood-fed (N), normal-blood-fed (B) and Plasmodium yoelii infected groups (I) was also analyzed by RT-PCR and gel scanning system at d1, d2, d3, d4, d7 and d11 after blood feeding. Results There is no significant difference of rpS7 signal between the three groups. Conclusion Anopheles dirus S7 can be used as an internal control to study the role of Anopheles dirus related immune factors in Plasmodium infection.
4.Research Advances on Triterpenoid Saponins Biosynthesis and Its Key Enzymes
Xiaoshuang XU ; Fusheng ZHANG ; Xuemei QIN
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2440-2448
As an important kind of plant secondary metabolites and widely distributed in the plant kingdom, triter-penoid saponins have a variety of biological activities. The constitution and content of triterpenoid saponin are deter-mined by some key enzymes and their expressing level in triterpenoid saponins biosynthesis pathway. So, it is very important to illuminate the molecular mechanism of triterpenoid saponins biosynthetic pathway. In recent years, illu-mination of the entire biosynthetic pathway especially the confirmation and cloning of the key enzymes, such as squa-lene synthase, squalene epoxidase, cytochrome P450 monooxygenase and UDP-glycosyltransferase, had become one of the hot spots by many scholars. In this paper, the entire biosynthetic pathway and some kinds of key enzymes in-volved in the synthesis of carbon skeleton, and its oxidation, and glycation were reviewed for further demonstrating the biosynthetic pathway of triterpenoid saponins and providing a theoretical basis for artificial biosynthesis.
5.Investigation of FoxP3 expression in peripheral blood and liver tissue of patients with hepatitis B
Dongping XU ; Junliang FU ; Fusheng WANG
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
Objective To investigate forkhead/winged helix transcription factor (FoxP3) expression in peripheral blood and liver-infiltrating lymphocytes (LIL) of patients with hepatitis B. Methods Flow cytometry, immunohistochemistry and real-time RT-PCR were employed for Fox P3 analysis of blood or live tissue obtained from patients with acute hepatitis B (AHB), chronic hepatitis B (CHB) and chronic severe hepatitis B (CSHB), as well as health controls. Results Fox P3 mRNA and protein expressions were specifically identified in CD4+CD25+ regulatory T cells. The level of Fox P3 mRNA in CD4+ T cells was identical with that in CD4+CD25+ regulatory T cell in peripheral blood. In CSHB patients a significant increase of Fox P3 mRNA expression was found in peripheral blood. The mean relative FoxP3 mRNA levels of CD4+ T cells in CSHB patients, AHB patients, CHB patients, and healthy controls were 0.199?0.174, 0.053?0.017, 0.059?0.053, and 0.056?0.021, respectively (CSHB group vs. other groups, P all
6.Dynamic detection of RNA of SARS-associated coronavirus in blood and excreta of SARS patients
Zheng ZHANG ; Dongping XU ; Fusheng WANG
Medical Journal of Chinese People's Liberation Army 1983;0(05):-
Objective To serially examine virus carrying condition of clinical specimens collected from SARS patients, in order to evaluate the dynamic changes of virus in the bady and its clinical significance. Methods A total of 494 samples, including plasma, urine, feces, sputum and throat swab, obtained from 84 cases of clinically diagnesed SARS patients at different time-points were examined for the study. Nested RT-PCR was employed for the detection of SARS-CoV using 2 pairs of primers targeting P and N region of the viral genome. The amplified viral genomic fragments were confirmed by DNA sequence analysis. Results Specific bands for SARS-CoV were amplified from various specimens of some SARS patients. Highest positive rate was found in sputum compared with the others. However, simultaneous examination of more than two specimens increased detectable rate of the virus. Moreover, dynamic examination revealed that the highest positive rate occurred in the 2nd week after the onset of the disease and next to the highest during the 3rd to 4th week. However, dynamic detectable rates for different specimens were not identical. Conclusion Nested RT-PCR is a practicable method for the detection of SARS-CoV and helpful for the early diagnosis or confirmation of the disease. Dynamic examinations for SARS-CoV by combined employment of the N and P primers and by involving multiple specimens may significantly increase the positive detectable rate, therefore would be helpful in diagnosis and eratuation of therapeutic effect of SARS.
7.The effects of different treatments of tooth extraction wound on alveolar bone healing
Xu SUN ; Fusheng DONG ; Guiyun REN ; Ning LI ; Xudong ZHANG
Journal of Practical Stomatology 2017;33(4):557-559
Bilateral mandibular first molars of 36 rabbits were extracted.The tooth extraction wounds were treated by:platelet-rich fibrin +acellular dermal matrix(group A),platelet-rich fibrin(group B),acellular dermal matrix (group C) and without treatment (group D,the control) (n =9) respectively.The measurments of alveolar bone height and width showed that there were no significant differences among groups at different times(P > 0.05).Bone histomorphometry showed that at the 2th and 4th week,the best result was found in group A(P<0.01).While,at the 8th week,the result of group A was still better than that of other 3 ones (P < 0.01),but group B and C showed no significant difference(P > 0.05).The combination of PRF and ADM shows the most significant effect.
8.Comparison of Performances of Ag/AgCl Coating Electrodes and Pulverized Electrodes in Cerebral Application of Electrical Impedance Tomography
Meng DAI ; Xiuzhen DONG ; Fusheng YOU ; Xuetao SHI ; Canhua XU
Chinese Medical Equipment Journal 2004;0(09):-
Objective To compare the performance of novel Ag/AgCl pulverized electrodes with conventional Ag/AgCl coating electrodes in cerebral application of Electrical Impedance Tomography(EIT).Methods Based on the EIT system developed by our group,raw data were measured on a phantom and an adult volunteer,with Ag/AgCl coating electrodes and pulverized electrodes,respectively.The performances of electrode systems were evaluated in contact impedance and two other figures(the high-frequency SNR,HFSNR;the low-frequency SNR,LFSNR).Results Ag/AgCl pulverized electrode plays a better performance in resisting noise and keeping stabilization.Conclusion Ag/AgCl pulverized electrode develops the needs of imaging and monitoring human brains in EIT more availably.
9.Inhibition of CpG Oligodeoxynucleotide on the Development of Pre-erythrocytic Stage of Plasmodium
Jide CHEN ; Taoli ZHOU ; Wenyue XU ; Yan DING ; Fusheng HUANG
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(01):-
Objective To study the role of cytidine-phosphate-guanosine oligodeoxynucleotide(CpG ODN) on the development of Plasmodium liver stage.Methods Plasmodium yoelii BY265 18S rRNA was cloned,and the TaqMan real-time PCR was established on P.yoelii BY265 18S rRNA and mouse GAPDH as quantitative analysis model.The model was tested by the level of liver Plasmodium load with the liver cDNA in BALB/c mice infected by salivary gland sporozoites for 42 hours.Twelve BALB/c mice were randomly divided into CpG group,CpG control group and PBS control group which were injected respectively by ODN1826 30 ?g,ODN1826 control 30 ?g and 0.01 mol/L PBS 200 ?l via vena caudalis.Twenty-four hours later,each mouse was inoculated with 100 sporozoites.Mice were sacrificed in 42 hours after infection,and the liver load of Plasmodium was analyzed by TaqMan real-time PCR.Results The cloned Py BY265 18S rRNA gene showed 98% similarity to Py 17XNL.The quantitative analysis model consisted by 18S rRNA and GAPDH showed positive correlation between the level of liver Plasmodium load and the sporozoite inoculation dose to mice.The Plasmodium load in CpG ODN pre-treated mice was reduced to one fifth of the control group(0.28/1.33)(P
10.Relationship between hemolymph phenol oxidase and melanization of Plasmodium yoelii oocysts in Anopheles dirus
Wenyue XU ; Fusheng HUANG ; Xilin ZHANG ; Mingshu KUANG ; Jianhua DUAN
Journal of Third Military Medical University 2001;23(4):440-442
Objective To explore the relationship between hemolymph phenol oxidase and the melanization of Plasmodium yoelii oocysts in Anopheles dirus. Methods An Anopheles dirus-Plasmodium yoelii system was used Anopheles dirus were divided into 3 groups, that is, non-blood-fedding (N), normal-blood-fedding (B) and infected-blood-fedding (I). The activities of MPO and o-DPO in hemolymph from 3 groups were determined with native polyacrylamide gel electrophoresis (PAGE) and density scanning at 5, 7, 11 and 15 d after blood feeding. Results Both MPO and o-DPO activity were significantly higher in group I than group N and B (P<0.05). But with the melanization of Plasmodium yoelii oocysts, both MPO and o-DPO activity in group I were decreased in comparison with group N, especially on the 15 th day after infected-blood feeding. MPO and o-DPO activity in group B were significantly stronger than those of group N. Conclusion Blood feeding and infection of Plasmodium yoelii both can activate the cascade. The heamolymph phenol oxidase may play an important role in the melanization of Plasmodium yoelii oocysts in Anopheles dirus.