OBJECTIVE:To provide reference for the enhancing of the quality of hospital preparations.METHODS:The disqualified reports on Shanghai hospital preparations published in "Shanghai drug and medical equipment quality bulletin" between 2004 and 2007 were collected to investigate the main quality problems.RESULTS:A total of 105 batches(19 dosage forms were covered)of unqualified preparations were reported in spot checks,of which,74(70.5%)were external preparations,17(16.2%)oral preparations,and 14(13.3%)sterility preparations.The common unqualified items include package amount(34.5%),description(15.4%),content(15.4%),pH value(13.6%),and microbial limit(8.2%).The major quality problems were related to package containers and technology.CONCLUSIONS:It is suggested that the concerned department to tighten control on quality of hospital preparations aimed at the above-mentioned problems.

Aim To investigate the in vivo effects of cilostazol,a novel phosphodiesterase III inhibitor,on signaling regulation,that is PPARs and NF-?B on vascular cell adhesion molecule-1(VCAM-1) in aorta of diabetic rats.Methods Diabetes was induced by intraperitoneally injecting fasted SD rats with streptozocin(STZ) at the dose of 65 mg? kg~(-1).32 diabetic rats were randomly divided into three groups,diabetes mellitus group(DM,n=12),high dosage of cilostazol group(GX,27 mg?kg~(-1)?d~(-1),n=10) and low dosage of cilostazol(DX,9 mg?kg~(-1)?d~(-1),n=10),plus normal control group(NC,n=8).After treatment for 8 weeks,using immunohistochemitry(IHC),in situ hybridyzation(ISH) and electrophoresis mobility shift assay(EMSA),we determined expression of VCAM-1,translocation of P65 subunit and activity of NF-?B in aorta tissues.PPAR? and PPAR? were examined by RT-PCR or Real-Time PCR.Results ①Compared with NC group,diabetic groups had elevated VCAM-1 expression of protein and mRNA(P

Objective To understand patients with gestational diabetes mellitus (GDM)in postpartum dia-betes incidence,and analyze risk factors of postpartum diabetes.Methods 144 cases with GDMwere selected as the research objects,according to the 5 year follow -up results,they were divided into the abnormal glucose tolerance group,diabetes group and normal blood glucose group,blood glucose during pregnancy,HbA1c,before the number of pregnancy,the changes of BMI and age were compared,the risk factors of postpartum diabetes was observed.Results Gestational diabetes mellitus patients 5 years postpartum diabetes morbidity rate was 25%,Postpartum diabetes gesta-tional fasting blood glucose(5.6 ±1.2)mmol/L and HbA1c(4.3 ±1.6)% were higher than those of the abnormal glucose tolerance group(6.6 ±1.3)mmol/L,(6.7 ±2.4)% and normal blood glucose group (7.3 ±1.2)mmol/L, (8.2 ±2.9)%.The difference among the three groups had statistical significance(P <0.001);HOMA -beta index (82.4 ±39.5)were lower than the other two groups(P =0.001),Pregnancy HbA1c(OR =6.37,P =0.001)and fasting blood glucose(OR =4.32,P =0.003)as well as previous pregnancies (OR =5.51,P =0.006)were inde-pendent predictors of postpartum diabetesindications.Conclusion Pregnancy HbA1c and fasting blood glucose level can determine the risk of gestational diabetes mellitus patients with postpartum diabetes;Have a family history of dia-betes and previous history of pregnancy patients.The risk of postpartum diabetes mellitus increase.

Objective To optimize physical examination processes,to manage physical examination scientifically in order to meet the needs of medical staff.Methods Medical guidelines were updated by using modern information systems.Meanwhile,the internal structure of medical examination center was rearranged reasonably.The examination process was stuck on the wall which integrated people oriented idea.Results After process reengineering,the return rate of examination menu was 98.55％,project completion rate was 99.16％,one time completion rate was 98.41％,physical examination time was reduced to (1.80±1.50) h.The satisfaction degree of the physical examinees was also increased.All parameters had significant differences compared with the former.Conclusions Scientific process reengineering of health examination plays a scientific and informatization management role.It can improve physical examination quality,increase physical examination capacity,and achieve good social benefit.

Objective To investigate the effect and safety of the recombinant tissue plasminogen activator ( rt-PA) combined with reduced glutathione ( RGSH ) in the treatment of acute ST-elevation myocardial infarction ( ASTEMI) .Methods 110 cases of ASTEMI were randomly divided into the control group and the observation group,55 cases in each group.The control group was intravenously injected 50mg rt-PA once a day,while the observa-tion group was injected 1200 mg RGSH freeze-dried powder once a day based on the control group .The vascular reca-nalization rate,the changes of myocardial enzymes ,cardiac structure and function parameters before and after treat-ment and adverse reactions in two groups were compared .Results The recanalization rate of the observation group and the control group reached 92.73%and 89.09% respectively,the difference between two groups was not signifi-cant (P>0.05).After treatment,the levels of CK,AST,cTnT,cTnI and CK-MB in the observation group were signifi-cantly lower than those of the control group (P<0.05),while the level of SOD was significantly higher than the con-trol group(P<0.05).The LVEDd and LVESd in the observation group were (52.24 ±5.83)mm and (39.95 ± 7.06)mm respectively,which were significantly lower than those in the control group (P <0.05),while the EF [(63.25 ±9.38)%] was significantly higher than that of the control group (P<0.05).The total adverse reaction rate in the observation group was 10.91%,which was significantly lower than 21.82% in the control group ( P<0.05).The reinfarction rate in the observation group was 3.63%,which was significantly lower than 12.73% in the control group(P<0.05).Conclusion The rt-PA routine thrombolysis therapy combined with RGSH could improve the recanalization rate and left ventricular structure and function of the ASTEMI patients and has less adverse reac -tion.The curative effect is better than single rt-PA.

Objective:To Compare the two-dimensional gel electrophoresis(2D-PAGE)with cerebrospinal fluid(CSF)of Parkinson’s disease patients and control group(patients with headache),and to find out the disease-specific proteins(DSPs)which are closely correlated with Parkinson’s Disease. Methods:proteins extracted from CSF were run in immobilized pH gradient isoelectic focusing(IPG-IEF)at the first dimension,and then run in vertical flat sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PA GE)at the second dimension. The protein spots in gels were visualized by silver staining protocol followed by gel scanning and analysised by Pdquest 8.0 software.Then differentially displayed protein spots were searched and identifiyed by MALDI-TOF-MS.Results:Three DSPs were found and they were Protein-tyrosine phosphatase 1B,Apolipoprotein E and E3 ubiquitin-protein ligase. Conclusion:There were different protein expressing in 2D-PAGE of Parkinson’s disease and control group.These proteins may be the DSPs of Parkinson’s disease and may be taked as biomarkers of Parkinson’s disease.

Objective To investigate the effect of montelukast sodium chewable tablets joint Tongqiaobiyan particles in the treatment of children with allergic rhinitis.Methods 108 children with allergic rhinitis according to the methods were divided into A group and B group,54 cases in each group.Both A and B group were given Tongqiaobiyan particles,weight <25kg patients,each half bag(1g),3 times a day;weight >25kg patients,each bag, 3 times a day.At the same time the A group was given montelukast sodium chewable tablets:2 to 5 years old,each 4mg,once daily.6 to 14 years old,each 5mg,once daily.Period of treatment for two weeks.Total efficiency and snee-zing,nasal congestion,runny nose,itchy nose symptoms and dry cough improve time,the recurrence rate after one month,two months of the two groups were compared.Results After the treatment,94 cases were accomplished,falled off in 14 cases.Among them,the A group completed in 48 cases,falled off in 6 cases;The B group completed in 46 patients from,falled off in 8 cases.The total effective rate of the A group was 95.8%,which was significantly high-er than 78.3% in the B group(χ2 =6.513,P <0.05).The sneezing,nasal congestion,runny nose,nasal itching and dry caugh of the A group were relieved more significantly than the B group(t =2.628,3.30,2.74,3.58,3.09,all P <0.05).Followed up 1 month and 2 months,the recurrence rates of A group and B group were 6.5% and 20%, 15.2% and 25%,respectively,there were no significant difference between with the two groups(χ2 =1.122,1.338, all P >0.05).Conclusion Tongqiaobiyan particles jointly montelukast sodium chewable tablets integrative curative in treatment of allergic rhinitis can significantly improve clinical symptoms,and has no significant adverse reactions. This method should be popularized and applied.

Short stature refers to the individual of the same race,gender and age,whose height is lower than the average height for about 2 standard deviations or under the 3rd percentile in the similar living environment.Its etiology mainly includes growth hormone deficiency,hypothyroidism,idiopathic short stature,Turner syndrome and so on.Short stature can be treated in childhood with recombinant human growth hormone (rhGH).Clinical evidence suggests that doctors should pay attention to thyroid hormone levels before and after rhGH therapy,so that they can offer thyroxine timely and maintain normal thyroid function for the children with hypothyroidism,which can improve the efficacy of rhGH in children with short stature.

Objective To investigate the effect of tissue adhesive glue in the repairment of eyelid lacerations and surgical incisions in children.Methods Retrospective comparison trial in paediatric ophthalmology department.Children who presented for laceration and surgical incision repair between November 2015 and October 2016 were evaluated.Patients from 11 months old to 12 years old with a total of 458 patients were randomly allocated to either glue (258 cases) or sutures (200 controls) to repair their wound.Cases and controls were similar in age,wound length and width involved.Those with wound seriously infected and longer than 5 cm were excluded.Length of time to perform procedure,pain assessment of procedure by doctor,parent and child and cosmetic outcome in 3 months and 1 year were measured.Results Time taken to repair the wound was faster in the glue group (P<0.01).Doctors and parents and the children themselves rated glue repair as less distressing (P<0.01).Complications (wound dehiscence,infection and corneal damage) were the same in both groups (P>0.05).Cosmetic outcome was better in the glue group in 3 months and 1 year (P<0.05).Conclusion Tissue adhesive glue is faster and easier and less painful than suturing.Tissue adhesive glue has better cosmetic outcomes than suturing when used for the repair of eyelid lacerations and surgical incisions in children.

Objective To investigate the effect of lipoteichoic acid of bifidobacterium (BLTA) on the expressions of Fas/FasL in bearing melanoma B16 cancer mice. Methods Forty C57BL/6 mice were randomly divided into 4 groups (n=10),normal saline group (NS group),low-dose BLTA group (50 mg/L),middle-dose BLTA group (100 mg/L),and high-dose BLTA group (150 mg/L). Melanoma B16 cells (0.2 ml 5?109/L) were subcutaneously injected into the mice of the later 3 groups,and then,0.2 ml BLTA at corresponding doses was injected subcutaneously at the sites around the mass once a day for 10 d when the tumor (at least 2 mm?2 mm) was palpable. The NS group was given normal saline at same volume as in BLTA group. The changes of the killing activity of CTL was examined by MTT assay. The expression of the tumor-infiltrating CD4+ and CD8+ lymphocytes in tumor tissue were detected by immunohistochemistry. RT-PCR was used to detect the mRNA expression variance of the Fas and FasL in tumor and spleen tissue. The protein expression of Fas,FasL in tumor tissue and lymphocytes of spleen were detected by immunohistochemistry and Western blotting. Results Compared with the control group,BLTA significantly improved the CTL killing activity (P0.05). Conclusion BLTA enhances the killing activity of CTL by regulating Fas/FasL system,and thus,promotes apoptosis of tumor cells to suppress tumor immune escape in tumor-bearing mice,thereby it exerts anti-tumor effect further.