Objective To investigate the clinical efficacy of auricular plaster therapy for depression in newly diagnosed type 2 diabetes.Methods Sixty patients with depression in newly diagnosed type 2 diabetes were randomly allocated to group A of 18 cases, broup B of 22 cases and group C of 20cases. In addition to conventional hypoglycemic treatment, group A received psychological intervention; group B, auricular plaster therapy; group C, oral administration of fluoxetine hydrochloride capsules. Pre-/post-treatment changes in fasting blood glucose, two-hour postprandial blood glucose, glycosylated hemoglobin and the Hamilton Depression Scale (HAMD) score were observed in the three groups after eight weeks of treatment. The clinical therapeutic effects were compared between the three groups.Results There were no statistically significant post-treatment differences in fasting blood glucose, two-hour postprandial blood glucose and glycosylated hemoglobin among the three groups (P>0.05). There was a statistically significant pre-/post-treatment difference in the HAMD score in the three groups (P<0.05). There was a statistically significant difference in the HAMD score between groups B and A at two, four, six and eight weeks after treatment (P<0.05), between groups B and C at two weeks after treatment (P<0.05) and between groups C and A at two, six and eight weeks after treatment (P<0.05). The total efficacy rate was 77.8% in group A, 90.9% in group B and 90.0% in the control group. There was a statistically significant difference in the total efficacy rate between group B or C and group A (P<0.05).Conclusion Auricular plaster therapy plus medication is an effective way to treat depression in newly diagnosed type 2 diabetes.

OBJECTIVETo optimize pre-coated multiple-probe fluorescence in situ hybridization (FISH) to improve its efficiency in cytogenetic diagnosis of acute leukemia.

METHODSThe original multiple-probe FISH techniques were optimized by adjusting the cell density and adding a process of protease digestion. Cytogenetic anomalies were detected in 141 patients with acute lymphocytic leukemia (ALL) or acute myeloid leukemia/ myelodysplastic syndromes (AML/MDS) using the modified technique, and 35 of the patients were also examined using the original technique. The successful detection rate and positive site detection rate were compared between the modified and original techniques.

RESULTSModification of the pre-coated multiple-probe FISH technique resulted in an significant increase of the successful detection rate (from 85.3% to 100%) and the positive site detection rate (from 5.1% to 8.6%) in ALL patients; in AML/MDS patients, the successful detection rate was significantly improved from 67.4% to 99.8% and the positive site detection rate from 3.5% to 6.0% (P<0.01).

CONCLUSIONThe modified pre-coated multiple-probe FISH technique can significantly increase the diagnostic efficiency of cytogenetic abnormalities in leukemic patients.

Chromosome Aberrations ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Karyotyping ; Leukemia, Myeloid, Acute ; diagnosis ; genetics ; Myelodysplastic Syndromes ; diagnosis ; genetics

Objective To investigate the efficacy and safety of single-channel endoscopic nylon rope combined with hemostatic clip suture in the treatment of peptic ulcer and bleeding.Methods Patients in our hospital due to peptic ulcer bleeding and hospitalization emergency electronic endoscopy from January 1st,2001 to July 1st,2017 were enrolled in the study.They were treated the single-channel endoscopic nylon rope combined with hemostatic clip suture.The basic information,clinical data,surgical records combined and retrospective analysed.Results 16 patients were included in the study.Among all the patients,peptic ulcer was located in 6 cases of gastric angle,3 cases of gastric antrum,3 cases of gastric body,2 cases of duodenal bulb and stomach.Ulcer maximum 40 mm × 25 mm,an average of (23.0 ± 6.0) mm.All ulcer wounds in patients underwent single-channel endoscopic nylon rope combined with hemostatic clip pouch suture were completely closed,including 1 case of duodenal ulcer patients after bleeding,achieved hemostasis successfully when turned into our hospital intervention.The remaining 15 cases were no longer bleeding.1 cases of gastric antral and 1 cases of stomach were diagnosed as moderately differentiated adenocarcinoma and they received endoscopic resuscitation after successful elective surgery gastric antral cancer radical surgery.Conclusions Single channel endoscopic nylon rope combined with hemostatic clip sacking is a safe and effective method for the treatment of giant peptic ulcer and bleeding.It is worthy of clinical manifestation,especially for those with deep and perforated ulcer.