Objective To investigate the effect of adjuvant therapy of Shenfu Injection on NOD-like receptor protein 3(NLRP3)/cysteine-aspartic acid-specific protease 1(Caspase-1)mediated py-roptosis signaling pathway and inflammatory factor levels in rats with acute myocardial infarction(AMI).Methods A total of 40 rats were randomly divided into sham operation group,model group,betaloc group(0.9 mg/kg),and combination group(0.9 mg/kg betaloc combined with 6 mL/kg Shenfu Injection),with 10 rats in each group.The rats were treated by gavage continuously for 3 weeks.The levels of serum troponin Ⅰ(cTnⅠ),creatine kinase-MB(CK-MB),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in rats were detected before modeling,after modeling,and at 3 weeks of treatment.Echocardiographic parameters such as left ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),and myocardial infarction area were compared among groups after modeling and at 3 weeks of treatment.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and Western blot were used to detect the mRNA and protein expression levels of NLRP3 and Caspase-1 in the myocardium.Results Compared with the sham operation group,the levels of cTnⅠ,CK-MB,IL-6,IL-1 β,TNF-α,myocardial infarction area,and the expressions of NLRP3 mRNA and Caspase-1 mRNA in the model group were significantly increased after modeling and at 3 weeks of treatment(P＜0.05);compared with the model group,the levels of cTnⅠ,CK-MB,IL-6,IL-1β,TNF-α,myocardial infarction area,and the expressions of NLRP3 mRNA and Caspase-1 mRNA in the betaloc group and the combination group were significantly decreased at 3 weeks of treatment,and the above indicators in the combination group were significantly lower than those in the betaloc group(P＜0.05).Conclusion Adjuvant therapy of Shenfu Injection for AMI can fur-ther alleviate myocardial cell injury,shorten infarction size,and inhibit inflammatory response and pyroptosis activity.

Objective To investigate the effect of adjuvant therapy of Shenfu Injection on NOD-like receptor protein 3(NLRP3)/cysteine-aspartic acid-specific protease 1(Caspase-1)mediated py-roptosis signaling pathway and inflammatory factor levels in rats with acute myocardial infarction(AMI).Methods A total of 40 rats were randomly divided into sham operation group,model group,betaloc group(0.9 mg/kg),and combination group(0.9 mg/kg betaloc combined with 6 mL/kg Shenfu Injection),with 10 rats in each group.The rats were treated by gavage continuously for 3 weeks.The levels of serum troponin Ⅰ(cTnⅠ),creatine kinase-MB(CK-MB),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in rats were detected before modeling,after modeling,and at 3 weeks of treatment.Echocardiographic parameters such as left ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),and myocardial infarction area were compared among groups after modeling and at 3 weeks of treatment.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and Western blot were used to detect the mRNA and protein expression levels of NLRP3 and Caspase-1 in the myocardium.Results Compared with the sham operation group,the levels of cTnⅠ,CK-MB,IL-6,IL-1 β,TNF-α,myocardial infarction area,and the expressions of NLRP3 mRNA and Caspase-1 mRNA in the model group were significantly increased after modeling and at 3 weeks of treatment(P＜0.05);compared with the model group,the levels of cTnⅠ,CK-MB,IL-6,IL-1β,TNF-α,myocardial infarction area,and the expressions of NLRP3 mRNA and Caspase-1 mRNA in the betaloc group and the combination group were significantly decreased at 3 weeks of treatment,and the above indicators in the combination group were significantly lower than those in the betaloc group(P＜0.05).Conclusion Adjuvant therapy of Shenfu Injection for AMI can fur-ther alleviate myocardial cell injury,shorten infarction size,and inhibit inflammatory response and pyroptosis activity.

Extracorporeal cardiopulmonary resuscitation (ECPR) is a salvage therapy for patients suffering cardiac arrest refractory to conventional resuscitation, and provides circulatory support in patients who fail to achieve a sustained return of spontaneous circulation. ECPR serves as a bridge therapy that maintains organ perfusion whilst the underlying etiology of the cardiac arrest is determined and treated. Increasing recognition of the survival benefit associated with ECPR has led to increased use of ECPR during the past decade. Commonly used indications for ECPR are: age<70 years, initial rhythm of ventricular fibrillation or ventricular tachycardia, witnessed arrest, bystander cardiopulmonary resuscitation within 5 min, failure to achieve sustained return of spontaneous circulation within 15 min of beginning cardiopulmonary resuscitation. This review provides an overview of ECPR utilization, recent outcomes, risk factors, and complications of ECPR. Identifying ECPR indications, rapid deployment of extracorporeal life support equipment, and high-quality ECPR management strategies are of paramount importance to improve survival.

Anoectochilus roxburghii is a traditional Chinese medicine and natural health products. In the modern cultivation system, A. roxburghii is micropropagated in tissue culture, and the plants are transferred to soil cultivation for months. However, it remains unclear about the necessity of soil cultivation for the accumulation of health beneficial compounds. In this paper, we performed nontargeted metabolomic analysis using GC-TOF-MS and UPLC-Q-TOF-MS, on A. roxburghii plants at tissue culture stage or after 3 months of soil cultivation. The results showed that the primary metabolites such as alcohols and organic acids are abundant in the tissue culture plants. In contrast, polysaccharide, nucleoside, esters and secondary metabolites such as flavonoids, terpenoids were significantly accumulated in cultivated seedlings. Flavonoids and polysaccharides are considered as the principle effective components in A. roxburghii. Soil cultivation period is therefore essential for the accumulation of these metabolites.