Objective It was studied that the effects of the serum of rats administered rutin on the proliferation, differentiation and maturation of rat calvarial osteoblasts in Vitro. Methods Osteoblasts were obtained from the newborn calvaria of Wistar rats younger than 24 hrs. After the bone pieces were digested with collagenase-trypsin, osteoblasts were released and were fed with α-MEM containing 10% fetal bovine serum. 12 adult Wistar rats were given rutin intragastrically at 25 mg/100 g body weight and their serum was obtained after 1hour. The control serum was obtained by giving equal volume of physiological saline. The serums were supplemented into the culture media of rat calvarial osteoblasts by 2.5%, 5% and 10% respectively. The proliferation of ROB was analyzed by MTT reduction assay. The osteogenic differentiation and maturation were assayed by measuring alkaline phosphatase activity(ALP)and numbering the mineralized bone nodules. Results The value of A570 in 2.5% and 5% SR group were Significantly [(0.678±0.242)%、(0.796±0.155)%]higher than those of the control. 10% SR was toxic to ROB. 5% SR was stronger (63.35±5.20)μg/μl than 2.5% SR in increasing ALP activity and the number of mineralized bone nodules which were significantly higher in SR group than in the control. Conclusion The metabolites of rutin after oral administration stimulate the proliferation and osteogenic differentiation of osteoblasts. Because so far, the vast majority of the Chinese herbal medicine containing Rutin are oral. The metabolites of rutin after oral administration are likely to be the effective in anti-osteoporosis. However, the role of specific forms and modalities have yet to be studied in depth.

It was an dideal model of respiratory distress syndyomeinduccd in 59 rabbits by Ⅳ oleic acid injection. Pulmonary hemodynamics, cardiac impedance paramcters were measured, microemboli and leukocytcs were abtained directly from the pulmonary microcirculation by use of the retrograde perfusion method. The results showed that pulmonary hemodynamics were changed significantly, but did not correlate with development of pulmonary edema. It was found that basic impedance (Zo) increased, stroke volume (SV), cardiac output (CO) and cardiac index (CI) all decreased (P

Objective To establish a HPLC method for determination of benzylacetone in the rhododendron.Methods The HPLC analysis was carried out on Luna C18(250 mm×4.6 mm,5 μm) at 30℃temperature.The mobile phase was acetonitrile-water (64 ∶ 36),the flow rate was 1 ml/min,and the detection wavelength was 220 nm.Results The linear range of benzylacetone was 17.08～170.8 μg/ml (R=0.9998),the average recovery of this method was 99.81％,and the RSD was 1.46％.Conclusion The method is convenient,quickly and accurate,can be used for the quality control of rhododendron oil and their preparations.

OBJECTIVE:To preparation Ru'an mixture,and establish its quality standard and observe its therapeutic efficacy.METHODS:Ru'an mixture was prepared with Radix Bupleuri and Ramulus Cinnamomi and Radix Angelicae Sinensis as raw material.Radix Bupleuri,Radix Paeoniae Alba,and Radix Angelicae Sinensis were identified by TLC and the content of Tanshinol was determined by HPLC.RESULTS:The TLC spots of Radix Bupleuri,Radix Paeoniae Alba,and Radix Angelicae Sinensis were all clear.The linear range of Tanshinol was 0.203～2.030?g(r=0.999 8).The total effect rate in Ru' CONCLUSION:Ru'an mixture is reasonable in preparation technique,controllable in quality.

The paper probed into the building and application of the evaluation system for the target responsibility system centering on nursing units, in line with recent development hospital practices. It discussed the design of the target-based quantitative assessment standards for nursing units, and the methods of target-based evaluation for nursing units, which are used in the target responsibility evaluation of 21 nursing units of the hospital. Results of the practice prove that such responsibility system raises the nursing quality and efficiency, improves nurses' incentives and cooperativeness, as well as elevates the safety and creativity of nursing and its management efficiency.

Objective To construct a RhoA-siRNA expression vector and determine its role on the malig-nant behavior of HepG2 cells.Methods A RhoA-siRNA DNA fragment was synthesized and cloned into the expression vector of pGenesil-1.The constructed Rhon-siRNA DNA plasmid was stably transfected into HerG2 cells by lipofectamine,and then HepG2 cells were divided into the HepG2/RhoA-siRNA group (HepG2 cells were transfected with pGenesil-1-RhoA-siRNA),HepG2/control group(HepG2 cells were transfected with control plasmid) and HepG2 group (without plasmid transfection).The inbibitory effect of RhoA-siRNA on RhoA protein expression was shown by Western blot.The proliferation,migration,growth potentiality and cell cycle of transfected HepG2 cells were evaluated by MTT assay,wounded healing,the plate cloning formation test and flow cytometry,respectively.All data were analyzed by one-way analysis of variance (ANOVA) and chi-square test.Results The expression of RhoA protein in the HepG2/RhoA-siRNA group was,significantly decreased compared with that in the other two groups (F=178.19,P<0.05).Scratched cells were healed within 48 hours in the HepG2/control group and HepG2 group,but not in the HepG2/RhoA-siRNA group.The clone formation rates in the HepG2/RhoA-siRNA group,HepG2 group and HepG2/control group were 39%±3%,67%±5%and 70%±6%,respectively,with a significant difference among the three groups(χ2=33.34,38.69,P<0.05).Flow cytometry showed that the number of cells transfected with RhoA-siRNA was highest in the G0/G1 phase and lowest in the S phase(F=70.46,76.57.P<0.05).Conclusion The RhoA-siRNA expression vector can effectively suppress the proliferation and migration of HepG2 cells,which may provide a novel gene therapy for hepatocellular carcinoma.

Wearable devices bring us an innovative human-computer interaction which plays an irreplaceable role in enhancing the users' ability in environmental awareness, acquirements of their own state and "ubiquitous" computing power. Since 2013, wearable devices have quickly appeared around us. In this article we classify most of the wearable devices which have been appeared in the markets or reported in the literature according to their functions and the positions where they are worn. Furthermore, we review the technologies related to wearable devices, such as sensing technology, wireless communication, power manager, display technology and big data. At last, we analyze the challenges which the wearable devices will face in near future, and look forward to development trends of wearable devices.
Biomedical Engineering ; Biomedical Technology ; Clothing ; Humans ; Monitoring, Ambulatory ; instrumentation ; Wireless Technology

OBJECTIVE: To explore the blood supply of the pedicle nasal septum mucosa flap of rabbit, in order to supply the theory and experiment basis for making the pedicle nasal septum mucosa flap to repair nasal cavity and skull base defect. METHOD: Twenty rabbits corpses were induced into the experimental subject, and inject 5 ml blue ink into the external carotid artery, then longitudinal cut apart the middle head of rabbit, finally observe the blood supply of the pedicle nasal septum mucosa flap. RESULT: The blood supply of the pedicle nasal septum mucosa flap mostly come from the vessels of extremitas anterior part of nasal septum. CONCLUSION Keep the he vessels of extremitas anterior part of nasal septum can guarantee the blood supply of mucous membrane, enhance the survival rate of nasal septum mucous membrane.
Animals ; Graft Survival ; Male ; Nasal Cavity ; surgery ; Nasal Mucosa ; blood supply ; Nasal Septum ; Rabbits ; Skull Base ; surgery ; Surgical Flaps ; blood supply ; Wound Healing

ObjectiveTo observe and compare the efficacy of two types of microencapsulated rat islets xenotransplanted into diabetic mice. MethodsThe mice diabetic model made with injecting 3% Streptozotosin through tail vein. Four groups were assigned: control group, naked islet transplantation group, alginate-BaCl2 microencapsulated islet transplantation group, agarose-PSSa microencapsulated islet transplantation group.300 islets were transplanted under the renal envelope of each diabetic mice respectively. ResultsThere were no significant difference in mean level of the blood glucose before transplantation among four groups. One week after transplantation, the respective mean level of the blood glucose in four groups were (7.26±1.56) mmol/L in alginate-BaCl2 microencapsulated islet transplantation group, (7.14±1.04) mmol/L in agarose-PSSa microencapsulated islet transplantation group, (7.42±1.52) mmol/L in naked islet transplantation group and (22.54±1.24) mmol/L in control. There were significant difference between the two encapsulated islet groups and the other two groups. The survived period of the two encapsulated islet transplantation groups were longer than that of the other two groups. The survived period of the alginate-BaCl2 microencapsulated islet transplantation group was longer than that of the agarose-PSSa microencapsulated islet transplantation group (92 d vs 56 d),the same as the time of keeping nomal blood glucose level (76 d vs 41 d). ConclusionMicroencapsulated rat islets with this two materials can survive in diabetic mice with their biological activity, and the alginate-BaCl2 microcapsules are better than the agarose-PSSa microcapsules.

Objective To develop a rapid donor liver harvesting method in orthotopic liver transplantation in rats,and to study the stability and the success rate of the model.Methods A total of 200 healthy adult male SD rats were randomly divided into two groups: the experimental group(rapid donor liver harvesting transplant group),and the control group(conventional liver transplant group).The operation time,cold(ischemic) time and warm ischemic time of the donor liver, recipient post-transplation liver function and the success rate of the operation between the 2 grous were cpmpared.Results In the control group,the(operation) time of donor harvesting was(33.8?4.2) min,warm ischemic time and cold ischemic time of donor liver was(3.5?1.2) min and(62.0?4.2) min,with a 80% rate of success.The new method reduced the time for donor surgery to(13.1?2.2)min,and reduced the warm ischemic time and cold(ischemic) time of donor liver to 0 min and(38.0?3.1)min respectively,and with a 94% rate of success(all P