Aim To investigate the effect of asiatic acid on apoptosis and autophagy in human glioblastoma T98G cells. Methods MTT colorimetry was employed to assay the cellular proliferating activity. The fluores-cence microscope and Hoechst 33258 staining were used to detect the morphological changes. The cell ap-optosis and autophagy were analyzed by flow cytometry with Annexin-V/7-AAD and MDC staining respective-ly. The expressions of associated proteins were detected by Western blot to analyze the mechanism of apoptosis and autophagy. Results MTT assay showed that the growth of T 9 8 G cells was inhibited by asiatic acid ( IC50 =46. 3 μmol · L-1 ) . Annexin V/7-AAD stai-ning and Western blot revealed that asiatic acid in-duced apoptosis in T98 G cells by reducing the expres-sion of Akt, decreasing the mitochondrial membrane potential, and increasing the expression of Caspase-3. MDC staining and Western blot showed that the per-centage of MDC-positive cells was decreased and the expressions of Beclin-1 , LC3-II and Atgs were inhibi-ted by asiatic acid treatment. 5 μmol·L-1 chloroquine was used to up-regulate the expressions of LC3-Ⅱand Beclin-1 . Asiatic acid-inhibited autophagy was blocked and the total apoptotic rate was reduced remarkably. Conclusion Asiatic acid suppresses T98 G cells pro-liferation by inducing apoptosis and inhibiting cell au-tophagy, and the very role of inhibiting autophagy could promote apoptosis to a certain extent.

BACKGROUND: With conversion of medical patterns and revolution of healthy concept, temperament, psychological health and behavior become key topics for child care and are paid more and more attention nowadays.OBJECTIVE: To survey the status quo of psychological and behavior problems and distribution of temperamental types through questionnaire for children in Guiyang.DESIGN: Randomized cluster sampling and cross-sectional study.SETTING: Guiyang Institute for Mother and Child Health.PARTICIPANTS: Twelve settings were randomly selected from 33 duty units for child care in Guiyang. Children aged 1-6 years suffered from health check at survey stage, and those with severe body diseases and abnormal intelligence were excluded.METHODS: Problems of psychological health and behavior and distribution of temperamental types for children were surveyed with self-made psychological health scale, Chinese temperament scale for 1-3-year children,Chinese temperament scale for 3-7-year children, behavior scale for 2-3-year children and Achenbach's children behavior scale standardized by Shanghai Mental Health Center. All questionnaires were answered by parents and taken back on the spot. After 2-4 weeks, several children were randomly selected to refill in the questionnaires mentioned above to measure reliability again.MAIN OUTCOME MEASURES: Status quo of psychological and behavior problems, distribution of temperamental types and effect of social environment on temperament and behavior.RESULTS: ① Distribution of temperamental types was divided into easy nurture, difficult nurture, nearly easy nurture, nearly difficult nurture and slow development, and the percentage was 35.58%, 4.21%, 39.70%, 7.37%and 13.14%. ② Total ratio of psychological problems was 94.50%, and ratios of children in various temperamental types were 98.91%, 97.26%,96.19%, 94.05% and 93.13% (P ＜ 0.01). ③ Total ratio of behavior problems was 19.57%, and ratios of children in various temperamental types were 31.76%, 26.69%, 22.58%, 21.33% and 12.72% (P ＜ 0.01). Temperamental types and behavior problems were related to living condition, educational level of parents, marriage, supervised attitude, paternity, pregnant nutrien, illness and infirmarian, especially temperament was closely related with psychological and behavior problems.CONCLUSION: Psychological and behavior problems wildly exist among children in Guiyang and should be paid much attention. Fine personality of children can be molded through reforming relevant factors so as to decrease the incidence of psychological and behavior problems.

Aim To investigate the effect of Bigelovii A on HepG2 cell apoptosis and its possible mechanism. Methods MTT assay was used to determine the inhibitory effect of Bigelovii A on HepG2 cells. Flow cytometry was performed to detect apoptosis. Western blot was applied to detect the expression of proteins of STAT pathway. Results BA (20 μmol L