Hepatitis A is one of the global public health problems,and China has high prevalence. In order to decrease the incidence of hepatitis A,China has integrated Hepatitis A vaccine into Expanded Program on Immunization (EPI) in 2007. The paper will provide some ideas on the immunogenicity and impact factors of the different types of hepatitis A vaccines that are used in China.

This paper discusses the brachytherapy with tumour interventional radiology technology.The structure and security of the intravascular iridium-192 sources afterloader are reviewed in comparison with general afterloader.

ERP system is introduced. Combined with the deficiencies of management of medical consumable materials in supply chain and high-value materials, the application of ERP to workflow, fundamental material, operation and high-value material management are explored. It indicates that although with some deficiencies, this ERP system optimizes business process and then improves work efficiency of management of medical consumable materials greatly.

Objective To collect the information about types and clinically-used materials for the dentures, and evaluate the current conditions and characteristics of prosthodontic treatment in Shanghai. Methods Three municipal public hospitals and two private commercial dental labs in Shanghai were served as sample units. The application information on fixed partial dentures, removable partial dentures and complete dentures from January 2005 to December 2007 was collected. The number of dentures in each sample unit, the total proportion of each type of dentures, and the proportion of each type of dentures in public hospitals or private labs were retrospectively analysed. Results From 2005 to 2007, the number of dentures increased by approximately 20% annually. The number of dentures made in private labs increased by 43.58% in 2005 to 2006 and 23.23% in 2006 to 2007.As to the types of dentures, fixed partial dentures constituted the largest proportion (74.49%-76.19% for public hospitals and more than 80% for private labs), while complete dentures accounted for the smallest proportion (5.07% to6.24% for public hospitals and 1.02% to 1.38% for private labs) . Conclusion With an ever-growing awareness of oral health, prosthodontics has been developing rapidly in Shanghai. The rapid increase trend of private dental clinics is noticeable. Fixed partial dentures constitute the largest proportion, while the proportion of complete denture gradually decreases year by year.

Objective To evaluate the left ventricular diastolic dysfunction by tissue Doppler imaging (TDI)in a suprarenal abdominal aortic coarctation model of Lewis rats. Methods Thirty male Lewis rats at age of 8 weeks were used. Myocardial hypertrophy model was obtained by suprarenal abdominal aorta banding with ligation. Mitral annular velocity measurement by TDI and regular two-dimensional echocardiography with M mode were examined at baseline and at 10-,20-,30-and 40-day post banding.Results Compared with baseline,the left ventricular end diastolic and end systolic wall thickness and ventricular dimension were progressively increased,while ejection fraction(EF)and fraction shortening(FS)were decreased(P＜0.0 1).The left ventricle demonstrated centrifugal myocardial hypertrophy.Peak myocardial systolic velocity(Sa)at the mitral annulus did not change significantly throughout this study and there was no significant correlation with EF(r=0.216,P=0.071),and peak myocardial early diastolic (Ea),late diastolic(Aa)at the mitral annulus and Ea/Aa indices also were not stable after aortic banding and there were no significant difference in repeated measures at different time(P＞0.05).But the E/Ea wasincreased and the index had a statistically significant difference(P＜0.001).Conclusions The left ventricleshowed centrifugal myocardial hypertrophy in Lewis rats with abdominal aortic banding.E/Ea radio was a sensitive and stable index to evaluate left ventricular diastolic dysfunction.

Objective To investigate the levels and clinical significance of monocyte chemoattractant protein (MCP)-1 and tissue factor (TF) in patients with acute exacerbation of chronic obstructive pulmonary disease(AECOPD).Methods Forty-nine elderly patients with AECOPD(AECOPD group) and 30 healthy controls (control group) were selected,and AECOPD group was divided into procalcitonin (PCT) increased group (PCT ≥ 0.5 μ g/L,19 cases) and PCT normal group (PCT ＜ 0.5 μ g/L,30 cases) according to the level of serum PCT.The levels of plasma TF,serum MCP-1 and PCT were determined by enzyme linked immunosorbent assay.Results The levels of plasma TF and serum MCP-1 were (203.6 ± 92.9),(152.8 ±99.9) ng/L in AECOPD group,and (136.9 ±24.3),(87.5 ±41.5) ng/L in control group.There were significant differences in the levels of plasma TF and serum MCP-1 between AECOPD group and control group (P＜0.01).The level of plasma TF was positively correlated with serum MCP-1 (r =0.673,P=0.029).The levels of plasma TF and serum MCP-1 were (215.3 ±71.2),(181.1 ±61.6) ng/L in PCT increased group,and (192.4 ±79.7),(137.3 ±74.4) ng/L in PCT normal group.There were significant differences in the levels of plasma TF and serum MCP-1 between PCT increased group and PCT normal group (P＜0.05).Conclusions In patients with AECOPD,hypercoagulability state is activated,and it is more severe in the patients with increased PCT.The level of plasma TF is positively correlated with serum MCP-1 in patients with AECOPD.To monitor the levels of plasma TF and serum MCP-1 is particularly important for elderly patients of AECOPD with hypercoagulability state to prevent cardiovascular,lungs and cerebrovascular thrombotic disease.

In our laboratory, injection of HGF, prepared by the modified Labrecque's method, was used for the treatment of experimental hepatic injury in rats. It was found that the serum estradiol level was significantly higher in the group of HGF treatment than that in the control group (P

Objective To explore the significance of peritubular capillary C4d deposition in histopathological changes, renal function and prognosis of the patients with antibody-mediated chronic rejection (AMCR). Methods Deposition of C4d in the kidney was examined by irnmunohistochemistry on routine paraffin-embedded sections using anti-C4d polyclonal antibody. Seventy-seven patients were divided into C4d+ group (n = 35) and C4d- group (n = 42). The relationship of C4d and renal function,histopathological changes and prognoses of allografts were analyzed. Results The number of patients with tubular atrophy and glomerular basement membrane proliferation in C4d+ group was significantly more than that in C4d group (P＜0.05). Mean serum creatinine level was significantly higher in C4d+ group than in C4d- group 12 months after renal transplantation [(379.1 + 260.2)μmol/L vs (260.5 + 175.3) μmol/L, P＜0.05]. According to Kaplan-Meier analysis, the one-year graft survival rate was lower in the C4d+ group (62.9% ) than in the C4d- group (83.3% ) (logrank P＜0.05). Conclusion Patients with C4d deposition are associated with tubular atrophy and glomerular basement membrane proliferation. The serum creatinine level in C4d+ patients was significantly higher than in C4d- group at the 12th month after transplantation. More patients with C4d deposition lost their grafts during the study period.

Glutathione S-transferase (GST) gene, PcgstA was cloned from the penicillin producing strain Penicillium chrysogenum,which is important for understanding the industrial fermentation process. PcgstA gene has an open-reading-frame of 840 bp in length,which is interrupted by two introns. The deduced amino acid sequence shows about 50% identity to several characterized filamentous fungi GSTs. The recombinant PcGSTA in Escherichia coli were overexpressed and purified. Enzymatic assays showed that the recombinant PcGSTA had a specific activity with 1-chloro-2, 4-dinitrobenzene of (0.159±0.031) μmol/(min· mg). It was found that the expression level of PcgstA in the penicillin producing medium supplemented with phenylacetic acid, the side chain precursor of penicillin G, was significant down regulated than that in medium without phenylacetic acid. This result suggested that PcGST may be related to phenylacetic acid metabolism in the penicillin producing strain.

ObjectiveTo investigate the efficacy of rapamycin combined with CsA/Tacrolimus (Tac) in chronic allograft nephropathy (CAN).MethodsFifty-three cases of CAN accepted the quadruple immunosuppressive drug program,which contained rapamycin combined with CsA/Tac and MMF and prednisone,and CsA/Tac and MMF were reduced to the original amount of 25％ to 50％.After treatment for 12 months,more relevant indicators,including serum creatinine,glomerular filtration rate,serum cholesterol,triglycerides,urinary protein,GPT and bilirubin and other changes were observed.ResultsIn the patients receiving quadruple regimen of rapamycin during 12 months,the blood Ccr was decreased from (161.51 ± 106.48)μmol/L before treatment to (126.51 ± 56.2)μmol/L after treatment for 6 months (P＜0.05) and to (123.43 ± 54.18)μmol/L after for 12 months (P＜0.01).The GFR was increased from (0.754 ± 0.302) ml/s before treatment to (0.952 ± 0.347)ml/s after treatment for 6 months (P＜0.05) and to (1.007 ± 0.394) ml/s after treatment for 12 months (P＜0.01).Cholesterol and triglycerides in patients had no significant change before and after treatment.The positive rate of proteinuria after treatment showed an increasing trend from 9.4％ before treatment to 26.4％ after treatment for 12 months.ConclusionThe quadruple program of rapamycin combined with CsA/FK506 and MMF can significantly improve Ccr and GFR in patients with CAN,but it can increase the incidence of proteinuria in patients: