Objective To observe the effect of different doses of atorvastatin on the plasma hydrogen sulfide (H2S) and nitric oxide (NO) levels in patients with essential hypertensive. Methods One hundred patients with essential hypertension in the People’s Hospital of Tunliu County from 2010-2012 were selected as research subjects. Thirty healthy patients were chosen as the control. The patients with essential hypertension were randomly divided into atorvastatin 40 mg/d group (n=38), atorvastatin 20 mg/d group (n=42) and control group (without atorvastatin, n=20) . The plasma H2S level of was detected by ELISA and serum NO by nitrate reductase assay. Results The plasma H2S and NO levels were lower in patients with essential hypertension than those in healthy subjects. The levels of H2S and NO in patients given atorvastatin (40 mg/d and 20 mg/d) were higher after treatment than before treatment. The differences of plasma H2S and NO levels between before and after treatment in atorvastatin 40 mg/d group and atorvastatin 20 mg/d group were higher than those in control group, and the differences were also higher in atorvastatin 40 mg/d group than those in atorvastatin 20 mg/d group. Conclusion The plasma H2S and NO levels were lower in patients with essential hypertension. Atorvastatin could increase the plasma H2S and NO levels in patients with essential hypertension.

Objective:To develop a safety protection device for lead cable of equipment in order to reduce or avoid the skin burn of patient and the invalidation of monitoring data caused by the short circuited of lead cable, and enhance the comfortable level of patient, at the same time, and reduce the pain of patient and potential adverse events.Methods: The device was consisted of the fixed sleeve of cylinder-shape (protective shell) and fixed sleeve of packaged cable (fixed sleeve of wire and cable), and its component was carbon fiber reinforced polycarbonate and silicone rubber. The fragile, repaired part or the rupture rubber of shell in upside and bottom of device were clamped in fixed sleeve. And then, the upside and bottom were fixed in one device, and take them socket with lead cable of device.Results: After the safety protection device of leading cable were used, the cable were fixed in the interior of the device, and could be protected. Therefore, some risks of adverse event were reduced and avoided.Conclusion: The device is stable in performance, is simple and convenient in operation and installation. In the application, it avoids the risk which caused by short circuit of cable in therapy monitoring, and could enhance the comfortable feel. This device is appropriate to various installation and application of lead cable, and it can be repeated to apply. It has got national patent for utility models due to its favourable application value.

ObjectiveTo investigate the clinical efficacy of hydroxyethyl starch 130/0.4 and sodium chloride injection in the treatment of patients with early hemorrhagic shock (HS).MethodsFifty-two cases of early HS were divided into compound sodium chloride injection group(control group,26 cases) and hydroxyethyl starch 130/0.4 sodium chloride injection group (treatment group,26 cases) by random digits table.The clinical effect,mean arterial pressure (MAP),respiratory rate (RR) and heart rate (HR) and serum sodium,serum chloride and prothrombin time(PT) were statistically analyzed before and after treatment for 60 min.ResultsCure rates in treatment group and control group were 84.6％ (22/26) and 38.5％ (10/26),and mortality rates were 15.4％ (4/26) and 61.5％ (16/26),respectively.The difference showed statistical significant(P ＜0.05).MAP,HR and RR in treatment group were obviously improved compared with control group[ (91.5 ± 8.6 )mm Hg( 1 mm Hg =0.133 kPa) vs.(78.5 ± 7.9 )mm H8,( 81.5 ±8.6) times/min vs.( 108.4 ± 10.2) times/min,(21.4 ± 5.3) times/min vs.(25.5 ± 4.6) times/min,P ＜ 0.05 ].Compared with control group,serum sodium,serum chloride and PT in treatment group were no significant difference(P ＞0.05).ConclusionHydroxyethyl starch 130/0.4 and sodium chloride injection could increase the effective circulating blood volume,and improve tissue and organ perfusion and reduce mortality during the patients with early HS.

Objective To understand thoroughly the effect of water improvement by investigating fluoride contents of drinking water in Meizhou fluorosis prevalent regions, the prevalence of fluorosis and the problems in water supply improvement projects. Methods In 2004, fluoride contents in drinking water were determined, 5 082 native pupils were examined for dental fluorosis according to Dean's method and the running status of water supply improvement projects was investigated also. Results After water supply improvement, the prevalence rate of the dental fluorosis in the fluorosis region(28.43 %) decreased significantly compared with that(56.99%) before the water supply improvement, the rate of fluoride content in drinking water that was up to standard reached 83.33%, the good running rate of water supply improvement projects was 68.42%. Conclusion The effect of water supply improvement is remarkable and satisfactory. In some regions little attention was paid to the management and daily maintenance of water supply but water source, which may cause water supply improvement projects damaged, in addition, water insufficiency and the alternate consumption of high fluoride water and normal water may be the main factors for fluorosis prevalence in some areas where although the water supply improvement has been finished.

Objective To observe the effect of retrograde sinus coronary perfusion on the function of myocardial cell membrane following retrograde sinus coronary perfusion Methods Thirty two isolated rabbit hearts were randomized into 4 groups: Group A, hearts were kept beating by retrograde perfusion with normothermic oxygenated blood; Group B, hearts received retrograde coronary sinus perfusion with warm blood cardioplegia; Group C, hearts received retrograde coronary sinus perfusion with cold blood cardioplegia; Group D, hearts received retrograde coronary sinus perfusion with cold crystalloid cardioplegia After 60 min retrograde perfusion, the hearts were switched on Langendorff Neely antegrade perfused working heart model for other 60 min Free calcium in myocardial cell, myocardial membrane MDA, ATPase and myocardial cell membrane fluidity were measured Results After retrograde coronary sinus perfusion, activity of ATPase and fluidity of the myocardial cell membrane in group A were higher than those in groups B, C and D The contents of myocardial cell membrane MDA and calcium in myocardial cell following retrograde perfusion were much lower in group A than those in groups B and C There was no remarkable difference between group C and group D Conclusion Retrograde perfusion of beating heart is superior to those perfused with warm blood cardioplegia in reducing free radical production, ameliorating paradoxical calcium accumulation in myocardial cell and maintaining activity of ATPase and fluidity of the myocardial cell membrane Warm blood cardioplegia is superior to cold blood cardioplegia

Objective To assess the effect of xiaojin wan plus nimesulide on patients with subacute granu-lomatoas thyroiditis(SAT). Methods 70 SAT cases were divided into nimesulide group(nimesulide 0.1 g,2/d) and combined group(nimesulide 0.1 g,2/d and xiaojin wan 1.2 g,2/d). When the patients' conditions were re-lieved after 3-4 weeks treatment,half doses of the medicines were given in two groups for 8 weeks. The efficacy,safe-ty and relapse rate after withdrawing medicines for 12 weeks were observed. Results The effective rates were 82.90% (29/35) in nimosulide group and 85.7% (30/35)in combined group, but both groups' effective rate was 50.0% (3/6) in patients with high temperature (higher than 39 ℃). The time for fever relieved in two groups were similar (P>0.05), but the thyroid pain smoothing time, normalized rates of blood sedimentation after 1 week therapy, enlarged thyroid or thyroid nodule after 3 week therapy, relapsed rate were significantly more efficacious in combined group than in nimesulide group (P<0.05). There was no severe side effects in the two groups. Conclu-sious There is a synergic effect of xiaojin wan plus nimesulide on patients with SAT, that is an effective and safe therapeutic regimen for SAT patients without high temperature.

Air pollution and global warming are increasingly deteriorating. Large amounts of polyamides derived from fossil fuel sources are consumed around the world. Cadaverine is an important building monomer block of bio-based polyamides, thus biotechnological processes for these polymers possess enormous ecological and economical potential. Currently, the engineered strains for biological production of cadaverine are Corynebacterium glutamicum and Escherichia coli. We review here the latest research progress of biosynthesis of cadaverine including metabolism of cadaverine in microorganisms, key enzymes and transport proteins in cadaverine synthesis pathway, optimum pathways and cadaverine yields.
Biosynthetic Pathways ; Biotechnology ; Cadaverine ; biosynthesis ; Corynebacterium glutamicum ; metabolism ; Escherichia coli ; metabolism

Objective To investigate the early diagnostic value of cornel confocal microscopy for the screening of small neuropathy in elderly patients with type 2 diabetic mellitus.Methods In the prospective study,96 elderly patients with diabetes as study group and 46 patients with non-diabetes as the control group were continuously collected from our hospital endocrinology and ophthalmology out patients during May 2014 to February 2016.The 96 cases of type 2 diabetes were subdivided into 47 patients with diabetic peripheral neuropathy (DPN)and 47 patients with nowdiabetic peripheral neuropathy(non DPN).Results The diabetes course was shorter in non-DPN group than in DPN group(P=0.000).The levels of glycosylated hemoglobin and urine albumin were lower in the nonDPN than in the DPN(P =0.072,0.007,respectively).The corneal nerve fiber density was lower in the DPN group than in NDPN group (P =0.000).Corneal nerve fiber density was higher in control group than in DPN and NDPN group.The differences in number of corneal nerve fibers showed no statistical significance between DPN and NDPN group (x2 =2.391,P =0.314).But the number of corneal nerve fibers was significant less in DPN and NDPN group than in control group(x2 =16.014,P =0.000).The negative correlation was found between the course of disease and corneal fibrous density by using single factor linear regression analysis.The number of corneal nerve fibers was lower in smoking group than in non-smoking group(P=0.003).The multiple linear regression analysis showed that duration of diabetes was a risk factor for diabetic neuropathy.Conclusions In some elderly diabetic patients with non-neuropathy,corneal nerve fiber density and number have been significantly decreased before nerve conductive velocity is reduced.Therefore,corneal confocal microscopy can be used to detect and diagnose small diabetic neuropathy in elderly patients with diabetes mellitus.

To improve 3-ketosteroid-△1-dehydrogenase (KSDH) activity and the transformation level for androst-4-ene-3,17-dione,3-ketosteroid-△1 -dehydrogenase gene (ksdD) from Arthrobacter simplex was cloned into plasmid pWB980 and expressed in B. subtilis WB600 under the control of promoter P43. The molecular weight of expressed enzyme was about 55kDa by SDS-PAGE analysis. The activitities assayed by spectrophotometrical method of intracellular and extracellular soluble enzyme were 110 ± 0.5mU and 15 ± 0.6mU per milligram of protein respectively. The transformation rate of androst-4-ene-3,17-dione by the B. subtilis recombinant cells was 45.3%. Compared with Arthrobacter simplex, the enzyme activity of KSDH expressed in B. subtilis was improved about 30 fold, and the transformation level of androst-4-ene-3,17-dione by the B.subtilis recombinant cells was improved about 10 fold. The recombinant B. subtilis cells used in biotransformation of steroids provided a new way for steroid medicines production.

OBJECTIVE:To study the feasibility of overflow dissolution method for evaluating the drug in vitro sustained re-lease performance. METHODS:Overflow dissolution method was established by simulating the drugs elimination in vivo. Using Nifedipine sustained-elease tablets(Ⅰ)from 2 different manufacturers as model drug A,B,concentration-time curve,cumulative release rate- time curve,release velocity-time curve of model drugs in release pool at 3 different overflow speed (0,1.50,3.00 mL/min)were investigated. RESULTS:When overflow speed was 0,the cumulative dissolution was consistent with that of the con-ventional dissolution method. As the overflow speed increased,cmax of drug A,B was decreased [A:(8.89±0.20),(5.21±0.04), (3.51±0.03)μg/mL;B:(7.62±0.05),(4.80±0.09),(2.89±0.04)μg/mL];cumulative release rate was increased [A:(85.47± 2.45)%,(94.29 ± 2.44)%,(96.04 ± 2.56)%;B:(73.28 ± 1.13)%,(78.46 ± 1.94)%,(82.50 ± 1.69)%] ;tmax was ahead (A:1.5,1.0,0.5 h;B:2.0,1.0,0.5 h). CONCLUSIONS:Overflow dissolution method has avoided the inhibition of too large drug concentration on drug release,making complete drug release and more accurate evaluation of in vivo sustained release performance of the preparation.