Alcoholic liver disease (ALD) is one of the most common chronic liver diseases in clinical practice with a complicated pathogenesis.Genetic factors play an important role in the pathogenesis of ALD and might be the potential targets for the treatment of ALD.This article reviews the susceptibility genes associated with ALD in recent years and points out that the exploration of the mechanisms of action of these genes in the development and progression of ALD can provide a theoretical basis for effective evaluation,diagnosis,and prevention and treatment of ALD in clinical practice.

Objective To observe the changes of behaviour, NOS neurons, Nissl bodies and ultrastructure after L-NNA treatment of acute spinal cord injury in rats. Methods Using movement and incline plane(IP)score to observe hindlimbs movement of rats with spinal cord injury; NADPHd histochemistry, Nissl methed and electron microscopy were used for observation of changes of neuronal NOS, Nissl bodies and ultrastructure of three groups(normal control group, saline solution(NS)control group and L-NNA group). Re- sults 1. Changes of behaviour: Hindlimbs movement and IP score in L-NNA group is more potent than that in NS group(P

ObjectiveTo explore the diagnostic value of serum YKL-40 for liver fibrosis stage, and to provide a reference for noninvasive diagnosis of liver fibrosis in patients with chronic liver disease. MethodsWe searched PubMed, EMBASE, the Cochrane Library, Web of Science, and CNKI for studies on the clinical value of YKL-40 in the diagnosis of liver fibrosis or cirrhosis. The quality of studies was evaluated using the QUADAS-2 tool to assess the risk of bias. Comprehensive quantitative evaluation of the included studies was performed using Stata 12.0. The source of heterogeneity was analyzed, and the forest plot and summary receiver operating curve (SROC) were generated. ResultsA total of nine studies involving 1592 patients were included in the meta-analysis; six studies were conducted on significant fibrosis (≥F2), and seven studies were conducted on progressive fibrosis (≥F3). In the diagnosis of significant fibrosis (≥F2), YKL-40 had pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, odds ratio, and area under the SROC (AUC) of 078 (95% confidence interval ［CI］: 0.69-0.85), 0.53 (95%CI: 0.33-0.72), 1.7 (95%CI: 1.0-2.7), 0.41 (95%CI: 0.21-0.76), 4 (95%CI: 1-13), and 0.76 (95%CI:0.72-0.80), respectively. In the diagnosis of progressive fibrosis (≥F3), YKL-40 had pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, odds ratio, and AUC of 0.83 (95%CI: 0.76-089), 0.72 (95%CI: 0.62-0.80), 3.0 (95%CI: 2.0-4.4), 0.23 (95%CI: 0.14-0.37), 13 (95%CI: 5-30), and 0.85 (95%CI: 082-0.88), respectively. ConclusionThe diagnostic value of serum YKL-40 for significant and progressive liver fibrosis is limited, so it may not be a new, effective serum marker for the staging of liver fibrosis.

OBJECTIVETo understand the distributions of DNA and neutralizing antibodies of human papillomavirus (HPV)16, 18 in 18-45 year-old women.

METHODSTotally, 1494 women were enrolled through multistage random sampling in Funing, Jiangsu province. Cervical exfoliated cells were collected from them for HPV DNA testing, and serum samples were taken from them for the detection of HPV16, 18 neutralizing antibodies by using pseudovirion-based neutralization assay(PBNA).

RESULTSAmong the 1494 women, 28(1.9%) and 188(12.6%) were positive for DNA and neutralizing antibody of HPV16 respectively, and 15(1.0%) and 60(4.0%) were positive for DNA and neutralizing antibody of HPV18, respectively. There were no significant differences in the detection rates of DNA and neutralizing antibody of HPV16, 18 among different age groups. About 16.7% of the women were infected with HPV16, 18, or both.

CONCLUSIONIn Funing county of Jiangsu province, most women aged 18-45 years has no immunity to HPV16 and 18, indicating that they are appropriate targets for HPV 16/18 vaccination.

Adolescent ; Adult ; Antibodies, Neutralizing ; isolation & purification ; Antibodies, Viral ; isolation & purification ; China ; DNA, Viral ; isolation & purification ; Female ; Human papillomavirus 16 ; immunology ; Human papillomavirus 18 ; immunology ; Humans ; Middle Aged ; Papillomavirus Infections ; prevention & control ; Papillomavirus Vaccines ; Young Adult

Objective To investigate the effect and mechanisms of pioglitazone on post operative cognitive function induced by surgery in elderly rats.Methods Elderly SD rats,male and weighing 220-250 g,were randomly divided into control group (group Con),surgery group (group S),pioglitazone group (group P)and pioglitazone/surgery group (group PS).The level of glycemia was measured by glucometer after completing the surgical model.The expression of advanced glycation endproducts (AGEs)in hippocampal tissues was detected by western blot and reactive oxy-gen species (ROS)and IL-6 by ELISA 1 2 h after surgery.Morris water maze was used for evaluation of cognitive function 4 days after surgery.Results Compared with group Con,group S showed a sig-nificant increase in the expression of AGEs and IL-6 and the level of ROS (P<0.05);Compared with group S,the expression of AGEs and IL-6 and the level of ROS decreased significantly in group PS (P<0.05).Compared to group Con,the surgery increased the average time of escape latencies on the 8th days and 10th days after surgery and reduced the platform-crossing times in the Morris water maze test (P<0.05).Compared with group S,group PS showed a significant decrease in the average time of escaping latencies on the 10th day after surgery (P<0.05),and an increased platform-cross-ing times in the Morris water maze test (P<0.05).Conclusion These results suggest that pioglita-zone attenuates postoperative cognitive function and its mechanism may be related to the decrease of the expression of AGEs and IL-6 and the level of ROS.