[Objective] Photographers perform a variety of tasks, including taking photographs for extended periods of time, using personal computers to organize photographic data, and preparing and transporting their equipment. Although photographers may exhibit a variety of symptoms, there have been no reports on investigations of pain or disease in photographers. In this study, we conducted a questionnaire survey on the actual status of work-related pain in photographers. We, then, performed acupuncture and evaluated the effects in subjects for pain or stiffness in the neck and shoulders, which was the most common chief complaint in the questionnaire.[Subjects and Methods] The survey was sent to 138 professional photographers. The survey included seven items, such as symptoms experienced during work activities and the content of the work. Among the respondents who reported chief complaints of pain in the neck and shoulder regions, six (one male and five females) opted to receive acupuncture treatment. We used the visual analog scale (VAS) scores to determine the amount of shoulder stiffness and tenderness in the shoulders and neck, as well as induration, before and immediately after treatment.[Results] Responses were obtained from 60.1% subjects. The most common chief complaint involved the neck and shoulder region in 86% of subjects. In the analysis of sex differences, the most common chief complaint in females was in the neck and shoulder region, whereas the most common chief complaint in males was in the lower back region. In the assessment of effects of acupuncture, the VAS scores for shoulder stiffness decreased after acupuncture (p < 0.05) and tenderness and induration scores decreased after acupuncture (p < 0.05).[Discussion and Conclusion] Low muscle strength may be a contributing factor for a higher complaint of neck and shoulder pain and stiffness in females. A higher complaint of pain and stiffness in the lower back region in males may be attributable to a higher load on the lower back region because they tend to have poor posture owing to height differences with the object being photographed. These results suggest that acupuncture is useful for work-related pain in the neck and shoulder region, which is the most common chief complaint in photographers.