The outbreak of skin lesions in workers cultivating okra (Hibiscus esculentus L.) was studied by a field survey. Questionnaire and physical examinations including patch tests with preparations of okra pods were carried out.
Out of 89 workers, 48 (53.9%) reported to have history of skin lesions. The sites of skin lesions were mainly arms (43.8%), dorsal regions of hands (35.4%), neck (33.3%), fingers and fingertips (each 29.2%). Concerning the symptoms of the skin lesions, itching (85.4%) and flare (45.8%) were dominant on picking work, and vanishing fingerprints and fissures on fingers on packing work. These clinical signs appeared shortly after the workers set to work without protective means.
Positive reactions of the patch tests with preparations of okra pods were found in 11 out of 89 okra workers (12.4%). The positive rate in non-okra workers was 3.4%. The results indicated that skin lesions due to okra were caused by primary irritation and allergic sensitization.

A simple and sensitive method for the determination of residual Nicarbazin (NCZ) by high performance liquid chromatography (HPLC) was examined to apply to livestock products. And the detection of residual NCZ in commercial chickens was also investigated.
1. The method that the acetonitrile extracts of meat homogenate were injected directly into HPLC was able to apply to determine the residual NCZ by adding process of degreasing and dehydration of the extracts. The average recovery of NCZ added to the chicken muscle (0.4 μg/g) were 89.13±6.61%(N =5) and detection limit in this analytical procedure was 0.02μg/g.
2. Using the above method for the analysis of NCZ in commercial samples, the frequency of detection was 13.0% and the range of concentration was 0.03-0.30μg/g in samples collected from Kagoshima city (N =131). The residual NCZ in commercial chickens showed a tendency to increase in summer season. The similar levels of frequency (12.5%) and concentrations (0.03-0.43μg/g) were found in samples collected from other prefectures (N=40).

In order to clarify the actual conditions and mechanism of contact dermatitis from pesticides, a field survey including patch testing with 11 pesticides and formaldehyde was carried out on 111 subjects who engaged in farming in the plastic greenhouse mainly. The relationship between skin sensitization due to pesticides and conditions of pesticide exposure was also studied. The results were as follows;
1) About half of subjects (52%) have been suffered from health hazards due to pesticides. Among the symptoms complained, dermatitis was most frequent (32%) as compared with acute poisoning such as headache (12%), general fatigue (9 %) and so on. The causative pesticides of those dermatitis were mainly Difolatan (72%), Daconil (8 %), and Gramoxone (8%).
2) Relatively higher rates of positive reaction of patch testing were observed in Difolatan (43%) in male, and Difolatan (25%), Topsin M (24%), Rovral (24%) in female. A positive reaction with more than one substance tested were seen in 31 males (55%) and 31 females (55%).
3) Among 11 kinds of pesticides tested, positive correlations between the skin reaction of patch testing and the use of corresponding pesticides were found only in Orthocide (r = 0.233) and Daconil (0.203).
4) From the Cluster Analysis, 16 kinds of using pesticides were classified into 3 clusters with similarity to patch test reactions. It was also suggested from the Dendrogram that there might be cross reactions among patch testing pesticides.
5) By Multiple Regression Analysis, no factor which contributed remarkably to skin sensitization was clarified. And, it was suggested that many kinds of factor were complicatedly related to skin sensitization.

An epidemiological survey on allergic conditions of 58 male (aged 38.6±11.8yrs) and 47 female (aged 42.2 ± 9.6yrs) farmers engaged in growing chrysanthemums.
Among the subjects, 47% of males and 62% of females had complaints of at least one of inquired allergic symptoms associated with the work. The immediate type of allergic reaction on nasal and/ or upper respiratory system raised in the process of sorting flowers in the working room and delayed ype of contact dermatitis in pinching the lateral bud of chrysanthemums or spraying agricultural chemicals in the field were the main complaints of those farmers.
Incidence of positive reaction for patch testing to six kinds of chrysanthemums was 5% for males and 17% for females and that of eight kinds at agricultural chemicals was 34% and 45% for each sex. And relatively high incidence was also observed in those subjects from other immunological tests such as determination of serum immunoglobulin, prick testing and calculation of eosinophil in blood and rhinorrhoea.
There were 39 (67%) males and 36 (77%) females who had at least one of the abnormal finding of those test items above mentioned. However, not the particular kind of Chrysanthemum cultivated in that area was found to be strong causative allergen for the observed allergic conditions.
From these results, it is indicated that relatively high incidence of allergic disorders may be found in the chrysanthemum growers due to inhalation and/or contact of some kinds of allergens introduced from the process of growing to forwarding, such allergen as Chrysanthemum itself, agricultural chemicals and other elements.

The outbreak of skin hazards from okra cultivation was studied by a field survey. About a half (46.2 %) of 186 workers investigated (male, 76 ; female, 110) reported to have experienced pastly or annually skin hazards from okra cultivation.The sites of lesion complained were mostly the arms (47.5%), neck (41.3%), fingers (32.5%) and so forth in total number. The degree of lesions were generally itching (85.0%) and flare (61.3%), but severe cases like vanishing fingerprints (16.3%) and fissures on the fingers (11.3 %) were also revealed. Portion of okra responsible for skin hazards seemed to be mainly leaves, trichomes and pods.
Positive patch test reactions with preparations of immature okra pods in 46 workers (male, 12 ; female, 34), compared to 112 control subjects (male, 84 ; female, 28), were significantly higher in okra workers than in controls, which were 25% in males and 20% in total group.
These results show that okra components cause irritant contact dermatitis and allergic contact dermatitis as well.