1.Simultaneous Determination of Imperatorin and Isoimperatorin in Yuanhu Zhitong Tablet by Capillary Electrophoresis
Hongquan GONG ; Chenghui ZENG ; Fuhai WU
China Pharmacy 2005;0(15):-
OBJECTIVE:To establish capillary electrophoresis method for the determination of imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS:The separation was performed on a uncoated fused silica capillary (55 cm?75 ?m ID,effective length of 47 cm) with 20 mmol?L-1 NaH2PO4+100 mmol?L-1 SDS+80%formamide as buffer solution (pH=7.12). The separation voltage was 15 kV and the sample was injected by gravity (5 s,15 cm). The detection wavelength was 254 nm. RESULTS:The linear range of imperatorin and isoimperatorin were 8.0~40.0 ?g?mL-1(r=0.998 7) and 4.0~20.0 ?g?mL-1(r=0.999 0). The average recovery was 101.3% for imperatorin (RSD=3.24%,n=6) and 100.2% for isoimperatorin(RSD=1.44%,n=6). CONCLUSION:The method is convenient,rapid and accurate for the quality control of Yuanhu zhitong tablet.
2.Content Determination of Shikonin in Arnebia euchroma by HPCE
Yan BAI ; Fuhai WU ; Bilian LUO ; Chunli ZENG
China Pharmacy 2005;0(19):-
OBJECTIVE:To determine the content of shikonin in Arnebia euchroma by HPCE-UV.METHODS:The separation was performed on uncoated fused silica capillary column (53 cm?50 ?m ID,46 cm effective length).2.0 mmol?L-1 H3BO3,6.0 mmol?L-1 triethylamine buffer solution and 5.0 mmol?L-1?-CD was used as background electrolyte,14 kV separation voltage and time of gravity injection 15 s (25 cm).The detection wavelength was set at 516 nm.RESULTS:The linear range of shikonin was 7.2~36.0 ?g?mL-1(r=0.999 0) with an average recovery of 100.15%(RSD=1.68%,n=6).CONCLUSION:The method is convenient,rapid,accurate and reliable for the content determination of shikonin in A.euchroma.
3.Values of CAD/CAM technology and assistant reconstruction in mandible defect with fibular musculocutaneous flap
Ming HU ; Lei ZHANG ; Hangyu ZHOU ; Lin JI ; Ziwei SONG ; Fuhai ZENG ; Yufei MA ; Delin XIA
Chinese Journal of Medical Aesthetics and Cosmetology 2018;24(6):391-395
Objective To explore the role of computer-aided design and manufacturing technology (CAD/CAM technology) in the repair of mandibular defects using the fibular osteomyocutaneous free flap.Methods Eight patients with a portion of mandible defect due to various reasons were subjected to preoperative CT scan,obtaining CT data of the mandible and the fibular bone to be transplanted.The original and the reconstructed skull model,as well as the mandible and fibula osteotomy guide plates,was printed using CAD/CAM technology.The titanium plates were preformed using the reconstructed skull model,and the operation was performed with the help of guide plates and a preformed titanium plate.The patients were followed up for 6 to 24 months to evaluate the operation effect.Results Two in 8 cases had flap necrosis caused by immune rejection of the implant,and one case had flap necrosis due to poor postoperative blood glucose control.The other 5 patients healed at the primary site and the fibular myocutaneous flap survived well.There were no serious complications in the donor and recepient site.The patients' satisfaction was high.Conclusions The reconstruction of mandible with the aid of CAD/CAM technique has the advantages of accelerating the operation process,shortening the operation time,improving the accuracy of mandibular amputation and fibular shaping,promotlng postoperative function and morphology recovery.
4.Development of a UHPLC-MS/MS method for the quantification of ilaprazole enantiomers in rat plasma and its pharmacokinetic application
Fengting OU ; Ying ZHOU ; Jinxiu LEI ; Su ZENG ; Fuhai WU ; Ning ZHANG ; Lushan YU
Journal of Pharmaceutical Analysis 2020;10(6):617-623
In Korea and China, ilaprazole is a widely used proton pump inhibitor in the treatment of gastric ulcers. In this study, a specific and sensitive LC-MS/MS method has been developed and validated for the quantification of ilaprazole enantiomers in the rat plasma, using R-lansoprazole as the internal standard. The enantioseparation was achieved on a CHIRALPAK AS-RH column (4.6 mm × 150 mm, i.d. 5μm), with a mobile phase composed of 10 mM ammonium acetate aqueous solution and acetonitrile (60:40, V/V), at a flow-rate of 0.5 mL/min. The method was validated over the concentration range of 0.5-300 ng/mL for both, R- and S -ilaprazole. The lower limit of quantification was 0.5 ng/mL for both enantiomers. The relative standard deviation (RSD) of intra- and inter-day precision of R-ilaprazole and S-ilaprazole was less than 10.9%, and the relative error accuracy (RE) ranged from -0.5%-2.0%. Finally, the method was successfully evaluated in rats in a stereoselective pharmacokinetic study of the ilaprazole racemate.
5.Effects of computer aided design and manufacture for reconstruction of mandibular defect with vascu-laried iliac crest flap graft
Fuhai ZENG ; Lei ZHANG ; Hangyu ZHOU ; Lilei REN ; Lin JI ; Yufei MA ; Delin XIA
Chinese Journal of Medical Aesthetics and Cosmetology 2019;25(3):210-213
Objective To evaluate the clinical application and effect of computerized digital sur -gical assisted design and manufacture for reconstruction of mandibular defect with vascularied iliac crest flap graft .Methods CT was used to scan the craniofacial and maxillofacial region of mandibular ameloblastoma patient ,and then the 3D model of the maxillofacial skeleton was reconstructed using the computerized digital surgical aid design to simulate the focal resection and the mandibular defect reconstruction with the iliac crest flap .Surgical guides were prepared by 3D printing to assist focal re-section and bone reshaping .Postoperatively ,clinical and CT examinations were performed to observe the restoration of the patient's facial appearance and occlusal relationship ,as well as wound healing and graft survival .Results Postoperative review results showed that the bone graft survived well ,and the anastomosed bone was formed .The wound in the surgery area healed .No recurrence of the tumor was found after clinical and CT examination .The maxillofacial appearance was well restored ,and the re-sidual occlusal relationship recovered well .Conclusions The use of computerized digital surgical aided design combined with 3D printing technology can effectively simplify the mandibular segmental defect repair and reconstruction and optimize the postoperative repair effect .
6. Feasibility and efficacy of indocyanine green used in laparoscopic gastrectomy for advanced gastric cancer patients
Shuai MA ; Yibin XIE ; Hongmei ZENG ; Quan XU ; Yuxin ZHONG ; Hao LIU ; Fuhai MA ; Fei ZHAO ; Huan LI ; Yang LI ; Yantao TIAN
Chinese Journal of Oncology 2019;41(12):904-908
Objective:
To identify the feasibility and efficacy of indocyanine green (ICG) used in laparoscopic gastrectomy for advanced gastric cancer patients.
Methods:
From December 2018 to August 2019, the clinical data of 82 patients preoperatively diagnosed as advanced gastric cancer undergoing laparoscopic radical gastrectomy were retrospectively analyzed. These patients were divided into ICG group(