Objective To compare their competence to repair peripheral nerve defect between lami-na propria glial cells and olfactory bulb glial cells. Methods Glial cells in nasal lamina propria and olfac-tory bulb had been cultured in vitro for 2 weeks, then purified and condensed for later transplantation. 60 adult wistar rats were randomized into three groups of 20 rats each: A (control), B (glial cells in olfactory bulb were transplanted into epineuria lumen) and C (glial cells in lamina propria were transplanted into epineuria lumen). Rats' left sciatic nerves were excised 25 mm long axons and retained epineuria lumen anastomosed to proximal ends. Culture mediums, glial cells in olfactory bulb and in lamina propria were transplanted into epineuria lumen of A, B, and C groups respectively. At 3 months postoperative,the regeneration of injured sciatic nerves were evaluated by methods of gross observation, microscopy, transmission electron microscopy, the retrograde fluorescence gold, the immunofluorescence assay of glial fibre acid protein and nerve growth factors, the enzyme linked immunoassay of myelin basic protein and neurofilament protein, and the extremity function scores. Results The regeneration of injured sciatic nerve and the function of injured limbs were su-perior in C group to in B group and in A group. The transportation distance of retro-marked fluorescence red and the concentrations of glial fibre acid protein, nerve growth factors, myelin basic protein and neurofila-ment protein in injured sciatic nerve were high in C group, middle in B group, and low in A group. The dif-ference between groups had statistical significance. Conclusion Olfactory ensheathing cells could repairinjured nerve defect and the competence that the olfactory cells in lamina propria were superior to in olfacto-ry bulb.

Objective To compare olfactory ensheathing cells (OECs) and Schwann cells (SCs) in respect of their abilities to repair nerve defects. Methods OECs and SCs were cultured in vitro for 2 weeks before they were purified and condensed for later transplantation. Sixty adult female Wistar rats were randomized into 3 even groups. After a 25 mm length of axons was excised but the epineurial lumen was re-tained, the left sciatic nerves were anastomosed to the proximal ends. Cell culture medium, SCs and OECs were transplanted into the epineurial lumen in A, B, C groups respectively. Three months postoperatively, regeneration of the injured sciatic nerves was evaluated by gross observation, microscopy, transmission elec-tron microscopy, transportation distance of retro-marked fluorescence red, immunofluorescence assay of the glial fiber acid protein and nerve growth factors, enzyme linked immunoassay of myelin basic protein and neurofilament protein, as well as ankle function evaluation. Results By gross observation, regeneration of the injured sciatic nerves was the best in group C and the poorest in group A. In aspects of growth of nerve fibers observed by microscopy and transmission electron microscopy, the transportation distance of retro-marked fluorescence red in the injured sciatic nerves observed by fluoroscopy, glial fiber acid protein and nerve growth factors tested by immunofluorescence assay, concentrations of myelin basic protein and neuro-filament protein in the injured nerves tested by enzyme linked immunoassay, as well as in the mean ankle function score, group C was the best, next was group B , and group A was the poorest. The differences be-tween groups had statistical significance (P < 0.05). Conclusion OECa transplantation can repair in-jured nerves and has better effects than SCs transplantation.

Objective To investigate the causes of misdiagnosis of B-ultrasonography in thyroid nodule and the function of B-ultrasonography in diagnosis in thyroid nodule.Methods 307 patients with thyroid nodule were analysis between the diagnosis of B-ultrasonography and pathology.Results The diagnosis of B-ultrasonography was different from the diagnosis of pathology in thyroid nodule. If the thyroid nodule found by B-ultrasonography was multiple, it was nodular goiter; If the thyroid nodule found by B-ultrasonography was single, the ratio of nodular goiter and thyroid adenoma was 3∶2.Conclusions Clinical physician did not grant the diagnosis of B-ultrasonagraphy as clinical diagnosis. Most of multiple thyroid nodules were nodular goiter, single mass may be nodular goiter or thyroid adenoma. When the diagnosis of B-ultrasonagraphy was thyroid occupation, it may be malignant thyroid neoplasm.

OBJECTIVE:To establish a RP-HPLC method for the determination of imperatorin in Qingwen jiedu tablets(heat-clearing and detoxifying agent).METHODS:The chromatographic separation was performed on Diamonsil-C18(250 mm?4.6 mm,5 ?m)with column temperature kept at 35 ℃.The mobile phase consisted of methanol-water(70∶30)at a flow rate of 0.8 mL?min-1 with detection wavelength set at 300 nm.RESULTS:The linear range of imperatorin was 2.7～27.0 ?g?mL-1(r=0.999 9)with its average recovery at 98.9%(RSD=1.44%,n=6).CONCLUSION:The established method was proved to be well-separated,sensitive,accurate and applicable for the quality control of Qingwen jiedu tablets.

Vascular smooth muscle cells (VSMCs) proliferation is the critical pathological process of in-stent restenosis (ISR). Recent researches indicate that the traditional Chinese medicine curcuma zedoary (E’zhu) can inhibit VSMCs proliferation, with a potential value in preventing and treating ISR. The major ac-tive components of curcuma zedoary are curcuma and β-elemene. The underlying mechanisms involve the inhibition of hemeoxygen-ase-1 expression, blockade of extracellular signal-regulated ki-nase – MAPK and Akt pathways, and subsequent cell cycle ar-rest. This article reviews the recent progress on curcuma zedoary extracts regulating VSMCs proliferation in ISR.

Objective To evaluate the effect of dexmedetomidine on the inflammatory responses in brain tissues in septic rats.Methods Seventy-two male Sprague-Dawley rats,aged 10 weeks,weighing 250-280 g,were randomly divided into 4 groups (n =18 each):control group (group C); sepsis group (group lipopolysaccharide (LPS)) ; distilled water group (group DW) and dexmedetomidine group (group D).Sepsis was induced by intraperitoneal injection of LPS 5 mg/kg (dissolved in normal saline 1 ml) in groups LPS,DW and DEX,while normal saline 1 ml was injected intraperitoneally in group C.Distilled water 20 μl was injected into the lateral cerebral ventricle in group DW,while dexmedetomidine 3 μg/kg (dissolved in distilled water 20μl) was injected into the lateral cerebral ventricle in group DEX.Six animals were sacrificed at 1,2 and 6 h after administration and hippocampi were removed for determination of TNF-α and IL-6 contents (by ELISA) and TLR4 mRNA expression in hippocampal tissues (by RT-PCR).Results Compared with group C,TNF-α and IL-6 contents in hippocampus tissues were significantly increased at each time point after administration in group LPS (P ＜ 0.05).Compared with group LPS,no significant change was found in TNF-α and IL-6 contents in hippocampal tissues (P ＞ 0.05),and TLR4 mRNA expression was significantly up-regulated at each time point after administration in group DW (P ＜ 0.05).Compared with group DW,TNF-α and IL-6 contents in hippocampal tissues were significantly decreased at each time point after administration,and TLR4 mRNA expression was significantly up-regulated at 2 and 6 h after administration in group DEX (P ＜ 0.05).Conclusion Dexmedetomidine can reduce inflammatory responses in brain tissues in septic rats via down-regulating TLR-4 mRNA expression.

Objective To identify dosimetric predictors for the development of gastrointestinal toxicity in patients with pancreatic adenocarcinoma treated with TOMO radiotherapy .Methods From January 2014 to Janu-ary 2015 ,we analysed the medical records of 68 pancreatic cancer patients who received helical tomotherapy from Air Force General Hospital .The stomach and duodenum were contoured separately to determine their dose volume histogram(DVH)parameters.Chi-square test was employed to analyze the count data .Spearman correlation anal-ysis was used to analyze the relationship between occurrence of gastrointestinal toxicity and clinical and physical factors.Logistic regression models was performed to identify risk factors associated with gastrointestinal toxicity . Results The median follow-up was 9 months(4~16 months).18 patients experienced grade II acute gastroin-testinal toxicity ,1 patient experienced grade Ⅲ acute gastrointestinal toxicity , whereas 17 patients experienced grade II late gastrointestinal toxicity ,1 patient experienced grade Ⅲlate gastrointestinal toxicity .On UVA,the vol-ume,Dmean,D1,D3,D5,D10,V5 to V40,and V5′to V45′of duodenum were significantly associated with GradeⅡor higher gastrointestinal toxicity ( P <0.05 ) .The MVA, V45′of duodenum was independent predictor for gradeⅡor higher gastrointestinal toxicity(P<0.05).The ROC analysis also showed that V45′of 0.5cm3 was the optimal threshold to predict for gastrointestinal toxicity for the entire cohort .Conclusion V45′of duodenum is of greater importance in the judgment of occurrence of hypofractioned radiation -induced gastrointestinal toxici-ty.

Objective:The feasibility of a minimally invasive cosmetic transbilical laparotomy was studied in fifty-seven pediatric patients.The advantages of this technique will be discussed.Methods:The circumferential incision was made following the ring fold of the right umblilicus,which was less than 2700.The peritoneum was opened in the midline via rectus abdominis.Intra-peritoneal lesions were detected and explored.The surgical procedures were able to be carried out either inside or outside of the peritoneum.After closure of peritoneum,the umbilical incision was closed subcutaneously with an absorbable suture.Results:From January 2001 to January 2005,58 procedures via transumbilical route have been done in 57 pediatric patients.None of those cases required conversion to a traditional abdominal incision.The duration of surgery was from 20 to 170 minutes.All patients were discharged five days postoperatively and follow-up was conducted from 3 months to 4 years.No complications were observed in all but one patient.This patient had a leaking anastamosis.No visible scars were present and the cosmetic results were excellent.All patients had shorter hospital stay and lower operating cost.Conclusion:Transumbilical approach is a feasible,safe,economical,minimally invasive procedure with excellent cosmetic results which appears to be superior to traditional procedures.It suggests that the transumbilical route should be widely used clinically and might be applicable for elder children and possibly would be of benefit in the treatment of other conditions.

Objective To observe the occurrence of the complication in patients under going interventional therapy for cardiovascular diseases and analyze its causes.Methods The data records of 1015 patients with interventional therapy for cardiovascular diseases in our hospital from October,2003 to October,2004(aged between 7 and 85 years,585 males and 430 females) were analyzed.Among them,520 patients received percutaneous transluminal coronary angioplasty and stent implantation,160 underwent radiofrequency catheter ablation,201 with permanent cardiac pacing,121 received blocking for congenital heart disease,10 received intraartery stent implantation and 29 with other interventional therapy.Results The complication occurred in 7.58% of 1015 patients.A higher incidence of hypotension and aberrant reflex has been reported(2.66% of all,mortality rate 0.39%).Conclusions Interventional therapy for cardiovascular diseases is an effective treatment with some risk.Serious pre-operational appraisal,enriching the experience of the doctors and strict post-operational monitoring may reduce the occurrence of the complication.

OBJECTIVETo investigate psychological stress and influence factors on the mine emergency rescue personnel.

METHOD564 mine emergency rescue personnel from a rescue group were select as subjects, and 60 designers from a steel design institute were as controls. Self-made questionnaire and general job stress questionnaire were used to investigate the basic information, rescue history, psychosomatic symptoms, depression symptoms, daily job stress and negative emotions of emergency rescue personnel. SPSS17.0 software was used to analysis the psychological stress on the mine rescue personnel and its influence factors.

RESULTSThe detection rate (41.94%) of depression symptoms in rescue team was higher than that of controls (24.90%). The score of daily job stress was higher than that of logistical support. The older age group with higher negative emotional and daily job stress than the younger. The highest negative emotion was in age group of more than 40 years old. The highest score of daily job stress was in ≤30 years old. The score of depression and psychosomatic symptoms were higher than those of the college and the above. The scores of depression in group of duration of rescue <10 years was higher than that of duration≥10 years. The score of daily job stress is the lowest in rescue for 1 to 2 times per year and the highest in group of simulation training once a week. The score of daily job stress and depressive symptoms were getting higher with the extension of combat duty time. Age, hours of combat duty, training times a week, education and life events were the main affecting fectors on mental health of mine rescuers.

CONCLUSIONSMine rescuers have more psychological stress than generic population. The psychological stress of the mine crew is related to age, education, life events, training and combat readiness duty time.

Adult ; Cross-Sectional Studies ; Depression ; Disasters ; Emergency Responders ; psychology ; Humans ; Mental Health ; Mining ; Rescue Work ; Stress, Psychological ; Surveys and Questionnaires